Updated on 2025/07/02

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写真a

 
Kohei Osawa
 
Organization
YCU Medical Center Oral and Maxillofacial Surgery/Orthodontics Assistant Professor
Title
Assistant Professor
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Degree

  • 博士(医学) ( 横浜市立大学 )

Research Areas

  • Life Science / Biomaterials  / 歯科インプラント

  • Life Science / Tumor biology  / 口腔がん

Education

  • Yokohama City University   Graduate   Graduate School of Medicine

    2016.4 - 2020.3

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  • Yokohama City University   Graduate   Graduate School of Medicine

    2018.4 - 2020.3

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  • Tsurumi University   School of Dental Medicine   School of Dental Medicine

    2008.4 - 2014.3

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Research History

  • 横浜市立大学附属市民総合医療センター   歯科・口腔外科・矯正歯科   助教

    2025.4

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  • 横浜市立大学大学院医学研究科   顎顔面口腔機能制御学   客員研究員

    2024.4 - 2025.3

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  • 藤沢市民病院   歯科口腔外科   専門医長

    2024.4 - 2025.3

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  • 藤沢市民病院   歯科口腔外科   主任医師

    2022.4 - 2024.3

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  • Yokohama City University   Graduate School of Medicine, Graduate

    2020.4 - 2024.3

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  • 大和市立病院   歯科口腔外科   医長

    2020.4 - 2022.3

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  • Yokohama City University   Hospital, Dentistry/Oral and Maxillofacial Surgery/Orthodontics

    2017.4 - 2018.3

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  • 藤枝市立総合病院   歯科口腔外科   医員

    2016.4 - 2017.3

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  • Yokohama City University   Hospital

    2014.4 - 2016.3

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Professional Memberships

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Papers

  • Three cases of gingival cancer associated with peri-implantitis and their management Reviewed

    Oral Science International   21 ( 3 )   519 - 527   2024.9

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    Authorship:Lead author, Corresponding author  

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  • Optimization of blood and protein flow around superhydrophilic implant surfaces by promoting contact hemodynamics Reviewed

    Kitajima H, Hirota M, Osawa K, Iwai T, Saruta J, Mitsudo K, Ogawa T

    Journal of Prosthodontic Research   67 ( 4 )   568 - 582   2023.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    PURPOSE: We examined blood and protein dynamics potentially influenced by implant threads and hydrophilic/hydrophobic states of implant surfaces. METHODS: A computational fluid dynamics model was created for a screw-shaped implant with a water contact angle of 70° (hydrophobic surface) and 0° (superhydrophilic surface). Movements and density of blood and fibrinogen as a representative wound healing protein were visualized and quantified during constant blood inflow. RESULTS: Blood plasma did not occupy 40-50% of the implant interface or the inside of threads around hydrophobic implants, whereas such blood voids were nearly completely eliminated around superhydrophilic implants. Whole blood field vectors were disorganized and random within hydrophobic threads but formed vortex nodes surrounded by stable blood streams along the superhydrophilic implant surface. The averaged vector within threads was away from the implant surface for the hydrophobic implant and towards the implant surface for the superhydrophilic implant. Rapid and massive whole blood influx into the thread zone was only seen for the superhydrophilic implant, whereas a line of conflicting vectors formed at the entrance of the thread area of the hydrophobic implant to prevent blood influx. The fibrinogen density was up to 20-times greater at the superhydrophilic implant interface than the hydrophobic one. Fibrinogen density was higher at the interface than outside the threads only for the superhydrophilic implant. CONCLUSIONS: Implant threads and surface hydrophilicity have profound effects on vector and distribution of blood and proteins. Critically, implant threads formed significant biological voids at the interface that were negated by superhydrophilicity-induced contact hemodynamics.

    DOI: 10.2186/jpr.JPR_D_22_00225

    PubMed

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  • Effect of a biomimetic hybrid meso- and nano-scale surface topography on blood and protein recruitment in a computational fluid dynamics implant model Reviewed

    Kitajima H, Hirota M, Osawa K, Iwai T, Mitsudo K, Saruta J, Ogawa T

    Biomimetics   8 ( 4 )   376   2023.8

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  • A case of Epstein-Barr virus-positive mucocutaneous ulcer in the right tongue that disappeared after treatment for oral candidiasis Reviewed

    Nozato T, Ozawa T, Ozawa M, Osawa K, Hirota M, Mitsudo K

    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology   34 ( 1 )   62 - 65   2022.1

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  • Sporadic Burkitt lymphoma initially presented as orofacial manifestations in an 8-year-old boy: a case report and mini review Reviewed

    Ohashi N, Iwai T, Nakamori Y, Iida M, Osawa K, Sugiyama S, Kitajima H, Minamiyama S, Yamanaka S, Shiiba N, Mitsudo K

    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology   33 ( 2 )   204 - 210   2020.9

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ajoms.2020.08.008

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  • Reactive Fibrosis Precedes Doxorubicin-induced Heart Failure through Sterile Inflammation, and Pioglitazone Reduces Early Cardiac Remodelling Reviewed International journal

    Ryo Tanaka, Masanari Umemura, Masatoshi Narikawa, Mayu Hikichi, Kohei Osaw, Takayuki Fujita, Utako Yokoyama, Tomoaki Ishigami, Kouichi Tamura, Yoshihiro Ishikawa

    ESC heart failure   7 ( 2 )   588 - 603   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    AIMS: Doxorubicin (DOX)-induced heart failure has a poor prognosis, and effective treatments have not been established. Because DOX shows cumulative cardiotoxicity, we hypothesized that minimal cardiac remodelling occurred at the initial stage in activating cardiac fibroblasts. Our aim was to investigate the initial pathophysiology of DOX-exposed cardiac fibroblasts and propose prophylaxis. METHODS AND RESULTS: An animal study was performed using a lower dose of DOX (4 mg/kg/week for 3 weeks, i.p.) than a toxic cumulative dose. Histological analysis was performed with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling assay, picrosirius red staining, and immunohistochemical staining. The mechanism was analysed in vitro with a low dose of DOX, which did not induce cell apoptosis. Microarray analysis was performed. Differentially expressed genes were confirmed by enrichment analysis. Mitochondrial damage was assessed by mitochondrial membrane potential. The production of inflammatory cytokines and fibrosis markers was assessed by western blot, quantitative polymerase chain reaction, and ELISA. A phosphokinase antibody array was performed to detect related signalling pathways. Low-dose DOX did not induced cell death, and fibrosis was localized to the perivascular area in mice. Microarray analysis suggested that DOX induced genes associated with the innate immune system and inflammatory reactions, resulting in cardiac remodelling. DOX induced mitochondrial damage and increased the expression of interleukin-1. DOX also promoted the expression of fibrotic markers, such as alpha smooth muscle actin and galectin-3. These responses were induced through stress-activated protein kinase/c-Jun NH2-terminal kinase signalling. A peroxisome proliferator-activated receptor (PPARγ) agonist attenuated the expression of fibrotic markers through suppressing stress-activated protein kinase/c-Jun NH2-terminal kinase. Furthermore, this molecule also suppressed DOX-induced early fibrotic responses in vivo. CONCLUSIONS: Low-dose DOX provoked reactive fibrosis through sterile inflammation evoked by the damaged mitochondria.

    DOI: 10.1002/ehf2.12616

    PubMed

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  • Prostaglandin E2 receptor EP4 regulates cell migration through Orai1 Reviewed International journal

    Kohei Osawa, Masanari Umemura, Rina Nakakaji, Ryo Tanaka, Rafikul Md Islam, Akane Nagasako, Takayuki Fujita, Utako Yokoyama, Toshiyuki Koizumi, Kenji Mitsudo, Yoshihiro Ishikawa

    Cancer Science   111 ( 1 )   160 - 174   2020.1

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    The EP4 prostanoid receptors are one of four receptor subtypes for prostaglandin E2 (PGE2 ). Therefore, EP4 may play an important role in cancer progression. However, little information is available regarding their function per se, including migration and the cellular signaling pathway of EP4 in oral cancer. First, we found that mRNA and protein expression of EP4 was abundantly expressed in human-derived tongue squamous cell carcinoma cell lines HSC-3 and OSC-19. The EP4 agonist (ONO-AE1-437) significantly promoted cell migration in HSC-3 cells. In contrast, knockdown of EP4 reduced cell migration. Furthermore, we confirmed that knockdown of EP4 suppressed metastasis of oral cancer cells in the lungs of mice in vivo. Therefore, we focused on the mechanism of migration/metastasis in EP4 signaling. Interestingly, EP4 agonist significantly induced intracellular Ca2+ elevation not in only oral cancer cells but also in other cells, including normal cells. Furthermore, we found that EP4 activated PI3K and induced Ca2+ influx through Orai1 without activation of store depletion and stromal interaction molecule 1 (STIM1). Immunoprecipitation showed that EP4 formed complexes with Orai1 and TRPC1, but not with STIM. Moreover, the EP4 agonist ONO-AE1-437 phosphorylated ERK and activated MMP-2 and MMP-9. Knockdown of Orai1 negated EP4 agonist-induced ERK phosphorylation. Taken together, our data suggested that EP4 activated PI3K and then induced Ca2+ influx from the extracellular space through Orai1, resulting in ERK phosphorylation and promoting cell migration. Migration is regulated by EP4/PI3K/Orai1 signaling in oral cancer.

    DOI: 10.1111/cas.14247

    PubMed

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  • Intra-oral minor salivary gland tumors: A pathological study of 131 cases in the Japanese population Reviewed

    Hayashi Y, Iwai T, Sugiyama S, Osawa K, Yoshii H, Minamiyama S, Kitajima H, Hirota M, Mitsudo K

    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology   31 ( 6 )   424 - 427   2019.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ajoms.2019.06.007

    Web of Science

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  • Prognostic factors and treatment outcomes of advanced maxillary gingival squamous cell carcinoma treated by intra-arterial infusion chemotherapy concurrent with radiotherapy Reviewed

    Hayashi Y, Osawa K, Nakakaji R, Minamiyama S, Ohashi N, Ohya T, Iida M, Iwai T, Ozawa T, Oguri S, Koizumi T, Hirota M, Kioi M, Hata M, Mitsudo K

    Head & Neck   41 ( 6 )   1777 - 1784   2019.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/hed.25607

    Web of Science

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  • Retrograde superselective intra-arterial chemoradiotherapy for squamous cell carcinoma for buccal mucosa Reviewed

    Koizumi T, Minamiyama S, Hayashi Y, Osawa K, Ohashi N, Ohya T, Iida M, Iwai T, Oguri S, Hirota M, Kioi M, Mitsudo K

    International Journal of Radiation Research   17 ( 2 )   283 - 291   2019.4

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  • Peripheral osteoma arising from the lateral surface of the mandibular ramus Reviewed

    Osawa K, Iwai T, Sugiyama S, Kitajima H, Baba J, Oguri S, Hirota M, Tohnai I

    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology   30 ( 3 )   278 - 280   2018.5

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier Ltd  

    DOI: 10.1016/j.ajoms.2018.02.004

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MISC

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Presentations

  • インプラント周囲炎を契機に発症した歯肉癌の3例

    大澤昂平, 岡本喜之, 廣田誠, 光藤健司

    第26回日本顎顔面インプラント学会総会・学術大会  2022.11 

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    Event date: 2022.11

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  • プロスタンディン受容体EP4の口腔がん細胞遊走能への影響とメカニズム解明

    大澤昂平, 中鍛治里奈, 小泉敏之, 光藤健司

    第38回日本口腔腫瘍学会  2020.1 

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    Event date: 2020.1

    Presentation type:Poster presentation  

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  • Cetuximab併用超選択的動注化学放射線療法と頸部リンパ節転移(N3)への温熱療法の併用療法を行った頬粘膜癌の1例

    大澤昂平, 小泉敏之, 佐久間要, 飯田昌樹, 佐藤格, 中鍛治里奈, 野里朋代, 南山周平, 林雄一郎, 光藤健司, 藤内祝

    第22回関東・全身ハイパーサーミア研究会合同学術研究会  2018.3 

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  • プロスタグランディン受容体EP4 は Orai1 を介したCa2+ 流入によって口腔がん細胞の 遊走能を亢進させる

    大澤昂平, 中鍛治里奈, 小泉敏之, 光藤健司

    第64回日本口腔外科学会総会・学術大会  2019.10 

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  • 口腔がんにおいてプロスタグランディン受容体EP4はOrai1と複合体を形成し、細胞内Ca2+の上昇に関与する

    大澤昂平, 梅村将就, 中鍛治里奈, 吾妻由美香, 石川義弘

    第29回日本病態生理学会  2019.8 

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  • Prostaglandin E2 receptor EP4 regulates the cell migration via Orai1 in Oral cancer cells

    Kohei Osawa, Masanari Umemura, Rina Nakakaji, Kenji Mitsudo, Yoshihiro Ishikawa

    2019.9 

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  • Bonding strength of soft relining materials to thermoplastic resins

    Osawa K, Waki T, Kuroiwa A, Imaizumi N, Tanzawa A, Viswambaran, Colonel M, Tokue A, Shimpo H, Ohkubo C

    2011 IADR/AADR/CADR General Session  2011.3 

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  • 当科における口腔癌同時性重複癌の検討

    大澤昂平, 小栗千里, 大橋伸英, 高山香名子, 上田潤, 飯田昌樹, 岩井俊憲, 中島英行, 筑丸寛, 小泉敏之, 廣田誠, 來生知, 光藤健司, 藤内祝

    第34回日本口腔腫瘍学会総会・学術大会  2016.1 

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  • Bartter症候群を伴う下顎前突症患者の下顎骨形成手術における周術期管理の経験

    大澤昂平, 高須曜, 富永拓也, 岡本喜之, 光藤健司

    第35回日本顎変形症学会総会・学術大会  2025.6 

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  • 分子プレカーサー法により作製したジルコニアコーティングインプラントが骨および歯肉に及ぼす影響

    大澤昂平, 櫻井敏継, 飯沼陽平, 廣田正嗣, 大久保力廣

    第54回日本口腔インプラント学会学術大会  2024.11 

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  • プロスタンディンE2受容体EP4はOrai1を介して細動遊走を調節する

    大澤昂平, 中鍛治里奈, 小泉敏之, 光藤健司

    第76回日本口腔科学会学術集会  2022.4 

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  • 歯科治療を契機に発症したLemierre症候群の1例

    大澤昂平, 岡本喜之, 田代茂太, 山田明佳, 森田大輝, 藤本明, 松本龍, 川田賢介, 石川好美, 光藤健司

    第67回日本口腔外科学会総会・学術大会  2022.11 

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  • インプラント治療を契機に発症した口腔扁平苔癬の1例

    大澤昂平, 榎本雅宏, 廣田誠

    第43回日本口腔インプラント学会関東・甲信越支部学術大会  2024.2 

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  • みんなに知って欲しい顎骨壊死~定期検診で早期発見~

    大澤昂平

    藤沢市民病院 市民公開講座  2024.8 

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  • 内視鏡支援下に摘出した上顎洞内迷入インプラントの1例

    大澤昂平, 榎本雅宏, 廣田誠

    第53回日本口腔インプラント学会学術大会  2023.9 

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  • 左側下顎臼歯部に生じた多房性発育性歯牙腫の1例

    大澤昂平, 富永拓也, 森田大輝, 北原慎一郎, 藤本明, 加藤礼朗, 松本龍, 川田賢介, 光藤健司, 岡本喜之

    第68回日本口腔外科学会総会・学術大会  2023.11 

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  • Elucidation of the mechanism of prostaglandin E2 receptor EP4 that regulates the migration in oral cancer cells.

    Osawa Kohei, Umemura Masanari, Nakakaji Rina, Akane Nagasako, Hiroko Nemoto, Uchino Megumi, Mitsudo Kenji, Ishikawa Yoshihiro

    第98回日本生理学会大会  2021.3 

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  • 上顎臼歯部に生じた正角化性歯原性嚢胞の1例

    大澤昂平, 小澤知倫, 野里朋代, 林雄一郎, 南山周平, 廣田誠, 光藤健司

    第66回日本口腔外科学会総会・学術大会  2021.11 

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  • Prostaglandin E2 receptor EP4 induced Ca2+ influx from the extracellular space via Orai1 and regulates the cell migration in oral cancer cells.

    Osawa Kohei, Umemura Masanari, Nakakaji Rina, Mitsudo Kenji, Ishikawa Yoshihiro

    第97回日本生理学会大会  2020.3 

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  • 舌に生じた髄膜腫の1例

    大澤昂平, 小澤知倫, 野里朋代, 南山周平, 磯野仁志, 中鍛治里奈, 石黒敬大, 林雄一郎, 廣田誠, 光藤健司

    第65回日本口腔外科学会総会・学術大会  2020.11 

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Awards

  • 優秀研究発表

    2024.11   第54回日本口腔インプラント学会学術大会  

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  • 2021年度 Rising Scientist賞

    2022.4   日本口腔科学会  

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  • 第18回 鶴見大学同窓会論文賞

    2021.2  

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  • 優秀ポスター賞

    2020.1   第38回 日本口腔腫瘍学会総会・学術大会  

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  • 優秀賞

    2019.8   第29回 日本病態生理学会大会  

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  • 第39回 長尾学術奨励賞

    2014.3  

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Research Projects

  • 異種化合物同時コーティングによる新規多機能インプラントの創成

    2025 - 2028

    日本学術振興会 科学研究費助成事業  若手研究  2025年度 若手研究

    大澤昂平

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    Authorship:Principal investigator 

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  • 口腔がんにおけるEP4シグナルをターゲットとした革新的治療法の開発

    Grant number:Aptos  2021 - 2024

    日本学術振興会 科学研究費助成事業  若手研究  2021年度 若手研究

    大澤昂平

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  • 口腔がん細胞におけるプロスタグランディンE2受容体EP4の役割解明

    2020 - 2021

    日本学術振興会 科学研究費助成事業  研究活動スタート支援  2020年度 研究活動スタート支援

    大澤昂平

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Other

  • 歯科医師臨床研修指導歯科医

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  • ICD制度協議会 インフェクションコントロールドクター

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  • 日本口腔外科学会 専門医

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  • 日本外傷歯学会 認定医・指導医

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  • 日本歯科放射線学会 認定医

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  • 日本口腔インプラント学会 専門医

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  • 日本がん治療認定医機構 がん治療認定医(歯科口腔外科)

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Teaching Experience