記述言語：英語   掲載種別：研究論文（学術雑誌）  

Gingival tissues in human cadavers were examined the blood vessel diameter in the depths of the gingival pockets such as three groups: gingiva adjacent to a sulcus of 2 mm (Group 1)
gingiva adjacent to a 2- 4-mm sulcus (Group 2)
and gingiva adjacent to a sulcus of &gt
4 mm (Group 3). A meaningful significant difference was seen observed in gingival pocket side, intermediate and outer layer side regions of the gingiva. A meaningful significant difference was seen found in intermediate part and the outer layer of the gingiva in Group 3. Other gingival biopsies were performed on a human body donation specimen to examine CD-31 positive endothelial cells of blood vessels by an immnohistochemical method. Our results suggest that the periodontal probing depth reflect the blood vessel organization of human gingival tissue.

DOI： 10.2535/ofaj.88.103

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

The maxillary sinus (MS) in the maxilla bone is located near the orbit, the nasal cavity and the oral cavity; however, the positioning of the constituent bones is complex. The posterior superior alveolar branches of the maxillary artery and nerve are distributed in the lateral wall of the MS. The courses of these blood vessels and nerves are restricted by the morphology of the craniofacial bones, and the landmarks used in dental implant treatment of these courses mainly run along the lateral wall of the MS. In this study, 19 human cadavers with 34 sides of Japanese origin (ranging in age from 59-94 years, mean 77.7 +/- 9.8 years) were prepared for measurement of the MS, the superior alveolar artery and the infraorbital artery using cone beam computed tomography (CBCT). The posterior superior alveolar artery (PSAA) of the lateral wall of the MS can be classified into one of three groups based on the supply pattern. In the greatest number of cadavers, the PSAA ran mainly to the lateral surface of the zone between the superior border of the alveolar foramen and the inferior border of the MS (53.0%, 18/34). In others, the PSAA ran to the zone between the infraorbital foramen and the superior border of the alveolar foramen (17.6%, 6/34); in a third group, the PSAA ran to the zone between the inferior border of the MS and the greater palatine foramen (23.5%, 8/34). The lest of two sides are spread out in this area (5.9%, 2/34). CBCT is the most accurate tool to evaluate important anatomical parameters, such as the distance of the blood supply, for the implant of grafts in the floor of the MS during surgical procedures.

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

The formation of the maxillary sinus (MS) is tied to the maturation of the craniofacial bones during development. The MS and surrounding bone matrices in Japanese foetal specimens were inspected using cone beam computed tomography relative to the nasal cavity (NC) and the surrounding bones, including the palatine bone, maxillary process, inferior nasal concha and lacrimal bone. The human foetuses analysed were 223.2 ± 25.9 mm in crown-rump length (CRL) and ranged in estimated age from 20 to 30 weeks of gestation. The amount of bone in the maxilla surrounding the MS increased gradually between 20 and 30 weeks of gestation. Various calcified structures that formed the bone matrix were found in the cortical bone of the maxilla, and these calcified structures specifically surrounded the deciduous tooth germs. By 30 weeks of gestation, the uncinate process of the ethmoid bone formed a border with the maxilla. The distance from the midline to the maximum lateral surface border of the MS combined with the width from the midline to the maximum lateral surface border of the inferior nasal concha showed a high positive correlation with CRL in Japanese foetuses. There appears to be a complex correlation between the MS and NC formation during development in the Japanese foetus. Examination of the surrounding bone indicated that MS formation influences maturation of the maxilla and the uncinate process of the ethmoid bone during craniofacial bone development. © 2010 The Author(s).

DOI： 10.1007/s00276-010-0678-5

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

We observed the location of the posterior superior alveolar artery (PSAA) and nerve at the macroscopic level between the maxillary sinus (MS) and surrounding bone of the anterior region of the maxilla. This study was completed using cone beam computed tomography (CBCT) imaging of 19 human cadavers with 38 sides of Japanese origin (ranging in age from 59-94 years, mean 77.7 ± 9.8 years) that were prepared for this study. The bony canal structure of the inner surface of the maxilla was clearly apparent in our results, and the bony canals were classified into three types according to the structure along the course of the PSAA: canal-like, ditch-shaped tunnel and fragmented, and the lest sides were undefined. Calcitonin gene-related peptide (CGRP)-positive fibers were identified along the PSAA in the bony canal of the maxilla by immunohistochemistry. The presence of the bony structure and CGRP-positive nerve fibers along the PSAA suggests that there is risk to the PSAA during surgery involving graft implant in the floor of the maxillary sinus.

DOI： 10.2535/ofaj.87.123

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SOC ITALIANA ISTOCHIMICA  

Tenascin-X (Tn-X) belongs to the tenascin family of glycoproteins and has been reported to be significantly associated with schizophrenia in a single nucleotide polymorphism analysis in humans. This finding indicates an important role of Tn-X in the central nervous system (CNS). However, details of Tn-X localization are not clear in the primate CNS. Using immunohistochemical techniques, we found novel localizations of Tn-X in the interstitial connective tissue and around blood vessels in the choroid plexus (CP) in macaque monkeys. To verify the reliability of Tn-X localization, we compared the Tn-X localization with the tenascin-C (Tn-C) localization in corresponding regions using neighbouring sections. Localization of Tn-C was not observed in CP. This result indicated consistently restricted localization of Tn-X in CP Comparative investigations using mouse tissues showed equivalent results. Our observations provide possible insight into specific roles of Tn-X in CP for mammalian CNS function.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Science and Business Media LLC  

The discomallear ligament (DML) runs through a narrow space of bony petrotympanic fissure, which joins the articular disc of the temporomandibular joint (TMJ) and the malleus in the tympanic cavity. Previous report suggest that an anatomical feature gives rise to TMJ pain and dysfunction. Recently, the movement of the malleus caused by hypertension on the discomallear ligament is important to the function of the TMJ. The purpose of this study is to define its morphological features using the cone beam CT (CBCT) and anatomical dissection of Japanese cadavers. Petrotympanic fissure and DML were observed in 14 cadavers (eight males and six females). It is revealed that a wide tunnel-like structure was found on CBCT images in the middle region of the petrotympanic fissure to the malleus in the tympanic cavity consisting of mainly three types: a wide tunnel-shaped structure (29.2%, 7/24, type 1), a tunnel-shaped structure widely open in the entrance of the petrotympanic fissure to the mandibular fossa and gradually thinning out in the tympanic cavity (20.8%, 5/24, type 2), and a tunnel-shaped structure widely open in the entrance of the mandibular fossa, middle region with flat-shaped tunnel structure and narrow exit in the tympanic cavity (41.7%, 10/24, type 3). These structures between the entrance of the petrotympanic fissure and the exit at the tympanic cavity are important to define the limited movement of the malleus. Therefore, morphological feature of the ligaments in malleus may relate to TMJ pain, dysfunction and hearing function.

DOI： 10.1007/s00276-008-0327-4

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その他リンク： http://link.springer.com/article/10.1007/s00276-008-0327-4/fulltext.html

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER GMBH, URBAN & FISCHER VERLAG  

During the development of blood vascular systems in the masseter muscle, one functional property of the blood supply via capillaries is altered by the change in feeding pattern from suckling to mastication. The lymphatic vessel hyaluronan receptor-1 (LYVE-1) is a marker of lymphatic endothelial cells. The PECAM (CD31) is also an important marker of vascular endothelial cells and lymphatic cells. The mechanisms by which circulating lymphatic endothelial. cells from blood vessels in masseter muscle form a network of lymphatic capillaries and vessels functioning in jaw muscle movement remain unknown. In our results, LYVE-1- and CD31- positive reactions were located in almost identical, regions at the stages examined using double immunofluorescence staining. However, the level of protein for LYVE-1 and CD31 differed between superficial and deep regions in postnatal rat masseter muscle using Western blotting analysis. The different distribution of LYVE-1 and CD31 antibody reactions was found in the deep region in contrast to that of the superficial area in 3-7-week-old rat masseter muscles. Concomitant with the increased level of protein for CD31 in the deep region, many small vessels branch in this region during development in rat masseter muscle. Therefore, different levels of protein and immunohistochemical reactions for CD31- and LYVE-1-positive cells may reflect alterations in the functional properties of the blood supply and collection via capillaries due to the changes in feeding pattern. (C) 2008 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.aanat.2008.03.013

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER GMBH, URBAN & FISCHER VERLAG  

Tenascin-X (Tn-X) belongs to the tenascin family of glycoproteins and is clearly associated with the human connective tissue disorder Ehlers-Danlos syndrome. Recently, human single nucleotide polymorphism analyses showed that Tn-X is associated with schizophrenia. Tn-X-related central nervous system (CNS) disorder has been reported in recent years. However, details of Tn-X localization are not clear in the adult cerebral cortex and its meninges. Using immunohistochemical techniques, we found novel localizations of Tn-X in the leptomeningeal trabecula (TB) of adult mice and in the connective tissue of the choroid plexus (CP) in the brains of mice. Subsequent immunohistochemical studies showed complementary localization of Tn-X in the leptomeninges and CP. Localization of tenascin-C was not detected in the leptomeningeal TB or in the connective tissue of the CR These results might provide insight into the role of Tn-X in the pathogenesis of disorders in the CNS. (c) 2008 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.aanat.2008.04.003

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：FRONTIERS RES FOUND  

Calbindin positive (CB+) giant neurons are known to occur within the pulvinar nucleus in subhuman primates. Here, we demonstrate by combined retrograde tracing and immunocytochemistry that at least some of these are pulvinocortical relay neurons, and further report several distinctive features. First, in contrast with non-giant relay neurons, the giant neurons are often solitary and isolated from a main projection focus. The question thus arises of whether their cortical projections may be non-reciprocal or otherwise distinctive. Second, these neurons are positive for GluR4; but third, they are otherwise neurochemically heterogeneous, in that about one-third are positive for both parvalbumin (PV) and CB. Presumably, these subpopulations are also functionally heterogeneous. These results provide further evidence for the idea of multiple, interleaved organizations within the pulvinar; and they imply that thalamocortical projections are more disparate than has yet been appreciated. Finally, we found that giant CB+ neurons have a distinctive meshwork of large, PV+ terminations, prominent at the first dendritic branch point. In size and location, these resemble inhibitory terminations from the zona incerta or anterior pretectal nucleus (APT), as recently described in higher order thalamic nuclei in rats. One can speculate that giant neurons in the macaque pulvinar participate in a layer 5-APT-thalamus (giant neuron) extrareticular pathway, functionally distinct from the layer 6-reticular nucleus-thalamus network.

DOI： 10.3389/neuro.05/002.2007

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

We investigated the properties of the muscle spindle in the masseter muscle at an immunohistochemical level in rats fed for 6 weeks. Slow myosin heavy chain (MyHC) isoforms were measured and intrafusal fibers in the muscle spindle were studied to determine the relationship between the superficial and deep regions of rat masseter muscle after alternated feeding pattern. However, muscle spindles were found in both regions, mainly in the deep region of the posterior superficial region of masseter muscle. The total number of the slow fiber in the intrafusal fiber and number of muscle spindle in the deep region were high from 5 to 8 weeks old in spite of various dimensions of data such as di-ameter and the compositions of the intrafusal fiber. The relationship of the protein expression of slow MyHC in the two regions at 5 weeks old reversed five weeks later (10 weeks old). This period is an important stage because the mastication system in masseter muscle with muscle spindle may be changed during the alternated feeding pattern of suckling to mastication. The changes may be a marker of the feeding system and of the control by the tension receptor of muscle spindle in this stage of masseter muscle after postnatal development.

DOI： 10.2535/ofaj.84.99

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-LISS  

Like other thalamic nuclei, the primate pulvinar is considered not to have long-range intrinsic connections, either excitatory or inhibitory. Injections of biotinylated dextran amine (BDA) in the medial pulvinar, however, reveal retrogradely filled neurons up to 2.0 mm from the injection edge. Serial section reconstruction (n = 18) confirmed that retrogradely filled neurons projected to the injection site and showed that they had additional long-range collaterals within the posterior pulvinar. Arrays of small, beaded terminations occurred in multiple foci along the collaterals. Terminal arrays were up to 1.0 mm in length; foci were separated by about 0.7 mm. Somata were large (average area = 220 mu m(2)), and dendritic arbors were radiate and also large (about 1.0 mm in diameter), but without either the appendages of classical interneurons or the hairlike spines characteristic of radiate pulvinocortical projection neurons. Double labeling for BDA and parvalbumin (PV) or BDA and gamma-aminobutyric acid (GABA) indicated that these large neurons were positive for both PV and GABA. Double labeling for PV and GABA, or PV and glutamic acid decarboxylase 67 (GAD67) revealed a small number of similarly large neurons in the posterior pulvinar that were positive for both substances. Thus, we propose that these neurons are a novel class of inhibitory interneuron, longer range than the classic thalamic local circuit interneurons. Future questions include how these neurons relate to other inhibitory systems and specific postsynaptic populations and whether they are located preferentially within the posterior pulvinar, possibly related to the multimodal character of this thalamic region.

DOI： 10.1002/cne.21085

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-LISS  

Fiber connections of the lateral valvular nucleus were investigated in a percomorph teleost, the tilapia (Oreochromis niloticus), by tract-tracing methods. Following tracer injections into the lateral valvular nucleus, neurons were labeled in the ipsilateral dorsal part of dorsal telencephalic area, corpus glomerulosum pars anterior, dorsomedial thalamic nucleus, central nucleus of the inferior lobe, mammillary body, semicircular torus, valvular and cerebellar corpus, in the bilateral rostral regions of the central part of dorsal telencephalic area, dorsal region of the medial part of dorsal telencephalic area, habenula, anterior tuberal nucleus, posterior tuberal nucleus, and spinal cord, and in the contralateral lateral funicular nucleus. Labeled fibers and terminals were found in the ipsilateral cerebellar corpus and bilateral valvula of the cerebellum. Tracers were injected into portions of the telencephalon, pretectum, inferior lobe, and cerebellum to confirm reciprocally connections with the lateral valvular nucleus and to determine afferent terminal morphology in the lateral valvular nucleus. Telencephalic fibers terminated mainly in a dorsolateral portion of the lateral valvular nucleus. Terminals from the corpus glomerulosum pars anterior, central nucleus of the inferior lobe, and mammillary body showed more diffuse distributions and were not confined to particular portions of the lateral valvular nucleus. Labeled terminals in the lateral valvular nucleus were cup-shaped or of beaded morphology. These results indicate that the lateral valvular nucleus receives projections from various sources including the telencephalon, pretectum, and inferior lobe to relay information to the valvular and cerebellar corpus. In addition, the corpus glomerulosum pars anterior in tilapia is considered to be homologous to the magnocellular part of superficial pretectal nucleus in (C) 2004 Wiley-Liss, Inc.

DOI： 10.1002/cne.20150

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：KARGER  

The holocentrid corpus cerebelli ( CC) is composed of the dorsal (CCd) and ventral (CCv) lobes. In the present study, afferent connections of the CCd and CCv in holocentrid teleosts (Sargocentron rubrum and S. diadema) were examined by means of tract-tracing methods. Tracer injections into either lobe of the CC labeled neurons in the ipsilateral area pretectalis pars anterior et posterior, nucleus paracommissuralis (NPC), nucleus accessorius opticus and nucleus tegmentocerebellaris. Labeled neurons were also present in the bilateral nucleus lateralis valvulae (NLV), nucleus raphes, nucleus reticularis lateralis and inferior reticular formation, and in the contralateral inferior olive. Injections into the CCd labeled only a few neurons in the area pretectalis pars anterior et posterior, nucleus accessorius opticus and nucleus tegmentocerebellaris, whereas many labeled cells were seen in these nuclei after CCv injections. Injections into the CCv also revealed afferent connections that were not observed after CCd injections. The CCv injections labeled additional neurons in the ipsilateral torus longitudinalis and nucleus subeminentialis and in the bilateral nucleus subvalvularis and nucleus of the commissure of Wallenberg. These differences in afferent connections suggest functional differences between the CCd and CCv. After injections into the CCd, labeled neurons in the NPC were restricted to a medial portion of the nucleus. On the other hand, after injections into the CCv, labeled neurons were found throughout the NPC. Labeled neurons in the NLV were mainly located in its rostral portion following CCd injections, whereas labeled neurons were mainly distributed in the medial portion following CCv injections. These observations suggest topographical organizations of the NPC-CC and NLV-CC projections. Copyright (C) 2004 S. Karger AG, Basel.

DOI： 10.1159/000080244

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-LISS  

Fiber connections of the torus longitudinalis (TL) and target(s) of toral recipient tectal neurons (pyramidal cells) in the optic tectum were examined by tract-tracing methods in holocentrids. Injections into the stratum marginale (SM) labeled neurons in the stratum opticum and stratum fibrosum et griseum. superficiale (SFGS). They had superficial spiny dendrites, with a fan-shaped branching pattern in SM and a thick basal dendrite that gave rise to bushy horizontal branches at the boundary between the SFGS and the stratum griseum. centrale (SGC), where an axon and a thin dendrite arose. The axon terminated in a middle cellular layer of the SGC, and the thin dendrite ramified slightly deeper to this cellular layer. The SM injections also labeled cells in the ipsilateral TL. Injections into either the lateral or the medial part of TL labeled terminals in the ipsilateral SM and neurons in the bilateral nucleus paracommissuralis (NPC) and nucleus subvalvularis and ipsilateral nucleus subeminentialis. Only medial TL injections labeled cells in the ipsilateral SGC. These neurons had a basal dendrite that branched in the middle cellular layer of SGC, suggesting that they receive inputs from the pyramidal cells and project back to the TL to form a closed circuit. Only lateral TL injections labeled terminals in the corpus cerebelli. A visual telencephalic portion projects to the NPC and sublayers of SGC, where dendrites of the pyramidal cells and SGC neurons ramify. The present results therefore suggest that the TL and SM are components of an intricate circuitry that exerts telencephalic descending visual influence on the optic tectum and corpus cerebelli.

DOI： 10.1002/cne.10730

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-LISS  

Fiber connections of the carp torus longitudinalis were re-examined by means of tract-tracing methods. The torus longitudinalis projected mainly to the stratum marginale of the optic tectum, area pretectalis, and corpus cerebelli. The stratum marginale was anterogradely labeled only by biocytin, but not by horseradish peroxidase. Because the stratum is composed of extremely fine axons of the small toral neurons, this may be ascribed to different molecular weights of the tracers. The main afferent sources to the torus longitudinalis were the nucleus subvalvularis, which was located beneath the nucleus lateralis valvulae, the nucleus subeminentialis pars magnocellularis, and neurons along the posterior mesencephalo-cerebellar tract. Some labeled cells also appeared in the area pretectalis, nucleus paracommissuralis, optic tectum, and torus semicircularis. In a previous paper, it was incorrectly reported that the valvula cerebelli was the main source of afferents to the torus longitudinalis. Here we report the reason for the previous mistake in relation to the techniques employed. (C) 2003 Wiley-Liss, Inc.

DOI： 10.1002/cne.10560

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：KARGER  

The nucleus paracommissuralis (NPC) of teleosts is a relay nucleus of an indirect telencephalo-cerebellar pathway. However, cells of origin in telencephalic subdivisions and terminal patterns of the NPC fibers in the cerebellum remain unclear. We studied these issues by means of tract-tracing methods in a cichlid, tilapia (Oreochromis niloticus). After tracer injections into the NPC, retrogradely labeled cells were found bilaterally in dorsal and ventral regions of the area dorsalis telencephali pars centralis (dDc and vDc) and area dorsalis telencephali pars dorsalis (Dd). Anterogradely labeled terminals were found in a caudal part of the bilateral corpus cerebelli (CC). The labeled terminals were restricted in the granular layer, which can be divided into dorsal and ventral regions based on cytoarchitecture. We injected tracers separately into the three telencephalic portions (dDc, vDc, and Dd) and into the dorsal or ventral regions of granular layer in the caudal CC. The results revealed a topographical organization of the indirect telencephalo-cerebellar pathway. A medial portion of the NPC received fibers from the vDc and projected to the ventral region of the caudal CC. An intermediate portion of the NPC received fibers from the dDc and Dd, and in turn projected to the dorsal region of the caudal CC. A lateral portion of the NPC received fibers from the Dd and in turn projected to the dorsal region of the caudal CC. The Dc is known to receive visual input via the area dorsalis telencephali pars lateralis, and the Dd is presumably a multimodal telencephalic portion. The present study suggests that the indirect telencephalo-cerebellar pathway through the NPC might convey descending visual and multimodal information to the CC in a topographical manner. We also demonstrated other indirect telencephalo-cerebellar pathways through the nucleus lateralis valvulae and the area pretectalis. Copyright (C) 2003 S. Karger AG, Basel.

DOI： 10.1159/000069353

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