Updated on 2025/07/12

All information, except for affiliations, is reprinted from the information registered on researchmap.

写真a

 
Kohsuke Kataoka
 
Organization
Graduate School of Medical Life Science Department of Medical Life Science Associate Professor
School of Science Department of Science
Title
Associate Professor
Profile
<現在の研究内容>
・副甲状腺の機能調節と転写制御
・Maf転写因子ファミリーの機能解析
・膵β細胞におけるグルコース感知と機能維持
・表皮ケラチノサイトの分化と転写制御
External link

Degree

  • 理学博士 ( 東京大学 )

Research Areas

  • Life Science / Metabolism and endocrinology

  • Life Science / Molecular biology

  • Life Science / Cell biology

Papers

  • Glucocorticoids reduce Slc2a2 (GLUT2) gene expression through HNF1 in pancreatic β-cells. Reviewed International journal

    Yuka Ono, Kohsuke Kataoka

    Journal of molecular endocrinology   2024.12

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Glucose transporter type 2 (GLUT2), encoded by the Slc2a2 gene, is essential for glucose-stimulated insulin secretion (GSIS) in pancreatic islet β-cells, and low expression of GLUT2 is associated with β-cell dysfunction during the progression of type 2 diabetes in humans and animal models. Glucocorticoids are stress hormones that regulate inflammation and metabolism through glucocorticoid receptor (GR), a member of the nuclear receptor superfamily, and synthetic glucocorticoids are widely used for the treatment of inflammatory disorders. Prolonged exposure to glucocorticoids induces β-cell dysfunction and diabetes, but the effects of Slc2a2 gene repression in β-cells, if any, and the mechanisms involved, remains unclear. In the present study, we measured the expression of GSIS-related genes in the MIN6 β-cell line, and found that Slc2a2 mRNA expression was selectively reduced by dexamethasone (DEX), a synthetic glucocorticoid. Using bioinformatics and reporter assays, we identified two β-cell enhancers of the Slc2a2 gene, one within the first intron and another located approximately 40 kbp downstream of the transcription start site. The latter enhancer (designated as E3c) was responsible for the DEX-induced repression of the Slc2a2 gene. Of the previously identified β-cell-enriched transcription factors (NEUROD1, MAFA, HNF1α, and HNF1β) that activate the E3c enhancer, the transcriptional activity of HNF1α and HNF1β, responsible for maturity-onset diabetes of the young types 3 and 5, respectively, was repressed by DEX and GR. This functional link between HNF1α/HNF1β and GR should help elucidate the mechanism of glucocorticoid-induced β-cell dysfunction and diabetes.

    DOI: 10.1530/JME-24-0077

    PubMed

    researchmap

  • CREB activates the MafA promoter through proximal E-boxes and a CCAAT motif in pancreatic β-cells Reviewed

    Yuki Aida, Kohsuke Kataoka

    Journal of Molecular Endocrinology   2024.8

     More details

    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Bioscientifica  

    MafA is a key transcriptional regulator of pancreatic islet β-cell function. Its target genes include those encoding preproinsulin and the glucose transporter Glut2 (Slc2a2); thus MafA function is essential for glucose-stimulated insulin secretion. Expression levels of MafA are reduced in β-cells of diabetic mouse models and human subjects, suggesting that β-cell dysfunction associated with type 2 diabetes is attributable to loss of MafA. On the other hand, MafA is transcriptionally upregulated by incretin hormones through activation of CREB and its co-activator CRTC2. β-cell-specific expression of MafA relies on a distal enhancer element. However, the precise mechanism by which CREB-CRTC2 regulates the enhancer and proximal promoter regions of MafA remains unclear. In this report, we analyzed previously published ChIP-seq data and found that CREB and NeuroD1, a β-cell-enriched transactivator, bound to both the promoter and enhancer regions of human MAFA. A series of reporter assays revealed that CREB activated the enhancer through a conserved cAMP-responsive element (CRE), but stimulated MAFA promoter activity even when the putative CRE was deleted. Two E-box elements and a CCAAT motif which bind NeuroD1 and ubiquitous NF-Y transcription factors, respectively, were necessary for transcriptional activation of the MAFA promoter by CREB. Genome-wide analysis of CREB-bound loci in β-cells revealed that they were enriched with CCAAT motifs. Furthermore, promoter analysis of Isl1 gene encoding a β-cell-enriched transcription factor revealed that a CRE-like element and two CCAAT-motifs, but not E-box, were necessary for activation by CREB. There results provide clues to elucidate the detailed mechanism by which CREB regulates MafA as well as β-cell-specific genes.

    DOI: 10.1530/jme-24-0023

    researchmap

    Other Link: https://jme.bioscientifica.com/downloadpdf/journals/jme/aop/jme-24-0023/jme-24-0023.xml

  • MafA, NeuroD1, and HNF1β synergistically activate the Slc2a2 (Glut2) gene in β-cells. Reviewed International journal

    Yuka Ono, Kohsuke Kataoka

    Journal of molecular endocrinology   67 ( 3 )   71 - 82   2021.7

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Glucose transporter type 2 (GLUT2), encoded by the SLC2A2 gene, is an essential component of glucose-stimulated insulin secretion in pancreatic islet β-cells. Like that of the gene encoding insulin, expression of the SLC2A2 gene expression is closely linked to β-cell functionality in rodents, but the mechanism by which β-cell-specific expression of SLC2A2 is controlled remains unclear. In this report, to identify putative enhancer elements of the mouse Slc2a2 gene, we examined evolutional conservation of the nucleotide sequence of its genomic locus, together with ChIP-seq data of histone modifications and various transcription factors published in previous studies. Using luciferase reporter assays, we found that an evolutionarily conserved region (ECR) located approximately 40 kbp downstream of the transcription start site of Slc2a2 functions as an active enhancer in the MIN6 β-cell line. We also found that three β-cell-enriched transcription factors, MafA, NeuroD1, and HNF1β, synergistically activate transcription through this 3' downstream distal enhancer (ECR3') and the proximal promoter region of the gene. Our data also indicate that the simultaneous binding of HNF1β to its target sites within the promoter and ECR3' of Slc2a2 is indispensable for transcriptional activation, and that binding of MafA and NeuroD1 to their respective target sites within the ECR3' enhances transcription. Co-immunoprecipitation experiments suggested that MafA, NeuroD1, and HNF1β interact with each other. Overall, these results suggest that promoter-enhancer communication through MafA, NeuroD1, and HNF1β is critical for Slc2a2 gene expression. These findings provide clues to help elucidate the mechanism of regulation of Slc2a2 gene expression in β-cells.

    DOI: 10.1530/JME-20-0339

    PubMed

    researchmap

  • Nasturtium officinale Extract Suppresses Osteoclastogenesis in RAW 264 Cells by Inhibiting I kappa B-Kinase beta Reviewed

    Yukino Tsunekage, Masatoshi Takeiri, Yuri Yoshioka, Shinichi Matsumura, Yoshihide Kimura, Kohsuke Kataoka

    NATURAL PRODUCT COMMUNICATIONS   16 ( 6 )   2021.6

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/1934578X211020643

    Web of Science

    researchmap

  • PIASy is a SUMOylation-independent negative regulator of the insulin transactivator MafA. Reviewed

    Onishi S, Kataoka K

    Journal of molecular endocrinology   2019.10

     More details

    Authorship:Last author, Corresponding author  

    DOI: 10.1530/JME-19-0172

    PubMed

    researchmap

  • Ectopic expression of the transcription factor MafB in basal keratinocytes induces hyperproliferation and perturbs epidermal homeostasis Reviewed

    Masashi Miyai, Yukino Tsunekage, Michiko Saito, Kenji Kohno, Kenzo Takahashi, Kohsuke Kataoka

    EXPERIMENTAL DERMATOLOGY   26 ( 11 )   1039 - 1045   2017.11

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/exd.13364

    Web of Science

    PubMed

    researchmap

  • Transcription Factor MafB Coordinates Epidermal Keratinocyte Differentiation Reviewed

    Masashi Miyai, Michito Hamada, Takashi Moriguchi, Junichiro Hiruma, Akiyo Kamitani-Kawamoto, Hajime Watanabe, Mariko Hara-Chikuma, Kenzo Takahashi, Satoru Takahashi, Kohsuke Kataoka

    JOURNAL OF INVESTIGATIVE DERMATOLOGY   136 ( 9 )   1848 - 1857   2016.9

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jid.2016.05.088

    Web of Science

    PubMed

    researchmap

  • Phosphorylation of MafA enhances interaction with Beta2/NeuroD1 Reviewed

    Song-iee Han, Yukino Tsunekage, Kohsuke Kataoka

    ACTA DIABETOLOGICA   53 ( 4 )   651 - 660   2016.8

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00592-016-0853-1

    Web of Science

    PubMed

    researchmap

  • Gata3 cooperates with Gcm2 and MafB to activate parathyroid hormone gene expression by interacting with SP1 Reviewed

    Song-iee Han, Yukino Tsunekage, Kohsuke Kataoka

    MOLECULAR AND CELLULAR ENDOCRINOLOGY   411 ( C )   113 - 120   2015.8

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.mce.2015.04.018

    Web of Science

    PubMed

    researchmap

  • Dimeric combinations of MafB, cFos and cJun control the apoptosis-survival balance in limb morphogenesis Reviewed

    Natsuno Suda, Takehiko Itoh, Ryuichiro Nakato, Daisuke Shirakawa, Masashige Bando, Yuki Katou, Kohsuke Kataoka, Katsuhiko Shirahige, Cheryll Tickle, Mikiko Tanaka

    DEVELOPMENT   141 ( 14 )   2885 - 2894   2014.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/dev.099150

    Web of Science

    PubMed

    researchmap

  • MafB Interacts With Gcm2 and Regulates Parathyroid Hormone Expression and Parathyroid Development Reviewed

    Akiyo Kamitani-Kawamoto, Michito Hamada, Takashi Moriguchi, Masashi Miyai, Fumie Saji, Ikuji Hatamura, Keizo Nishikawa, Hiroshi Takayanagi, Seiji Hitoshi, Kazuhiro Ikenaka, Toshihiko Hosoya, Yoshiki Hotta, Satoru Takahashi, Kohsuke Kataoka

    JOURNAL OF BONE AND MINERAL RESEARCH   26 ( 10 )   2463 - 2472   2011.10

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/jbmr.458

    Web of Science

    PubMed

    researchmap

  • Proteasome activator PA28 gamma stimulates degradation of GSK3-phosphorylated insulin transcription activator MAFA Reviewed

    Kenichi Kanai, Shinsaku Aramata, Sayo Katakami, Kunio Yasuda, Kohsuke Kataoka

    JOURNAL OF MOLECULAR ENDOCRINOLOGY   47 ( 1 )   119 - 127   2011.8

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1530/JME-11-0044

    Web of Science

    PubMed

    researchmap

  • ATF2 Interacts with beta-Cell-enriched Transcription Factors, MafA, Pdx1, and Beta2, and Activates Insulin Gene Transcription Reviewed

    Song-iee Han, Kunio Yasuda, Kohsuke Kataoka

    JOURNAL OF BIOLOGICAL CHEMISTRY   286 ( 12 )   10449 - 10456   2011.3

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M110.209510

    Web of Science

    PubMed

    researchmap

  • Insulin Transactivator MafA Regulates Intrathymic Expression of Insulin and Affects Susceptibility to Type 1 Diabetes Reviewed

    Shinsuke Noso, Kohsuke Kataoka, Yumiko Kawabata, Naru Babaya, Yoshihisa Hiromine, Kaori Yamaji, Tomomi Fujisawa, Shinsaku Aramata, Takashi Kudo, Satoru Takahashi, Hiroshi Ikegami

    DIABETES   59 ( 10 )   2579 - 2587   2010.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2337/db10-0476

    Web of Science

    PubMed

    researchmap

  • SUMOylation negatively regulates transcriptional and oncogenic activities of MafA Reviewed

    Kenichi Kanai, Hasan Mahmud Reza, Akiyo Kamitani, Yuri Hamazaki, Song-iee Han, Kunio Yasuda, Kohsuke Kataoka

    GENES TO CELLS   15 ( 9 )   971 - 982   2010.9

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1365-2443.2010.01431.x

    Web of Science

    PubMed

    researchmap

  • c-Maf and MafB transcription factors are differentially expressed in Huxley's and Henle's layers of the inner root sheath of the hair follicle and regulate cuticle formation Reviewed

    Masashi Miyai, Yoshihiro G. Tanaka, Akiyo Kamitani, Michito Hamada, Satoru Takahashi, Kohsuke Kataoka

    JOURNAL OF DERMATOLOGICAL SCIENCE   57 ( 3 )   178 - 182   2010.3

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jdermsci.2009.12.011

    Web of Science

    PubMed

    researchmap

  • Mono-(2-ethylhexyl) phthalate Targets Glycogen Debranching Enzyme and Affects Glycogen Metabolism in Rat Testis Reviewed

    Chikanori Kuramori, Yasuyoshi Hase, Koichi Hoshikawa, Keiko Watanabe, Takeyuki Nishi, Takako Hishiki, Tomoyoshi Soga, Akihiro Nashimoto, Yasuaki Kabe, Yuki Yamaguchi, Hajime Watanabe, Kohsuke Kataoka, Makoto Suematsu, Hiroshi Handa

    TOXICOLOGICAL SCIENCES   109 ( 1 )   143 - 151   2009.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/toxsci/kfp041

    Web of Science

    PubMed

    researchmap

  • Presentation of functional foreign peptides on the surface of SV40 virus-like particles Reviewed

    Ryou-u Takahashi, Shin-nosuke Kanesashi, Takamasa Inoue, Teruya Enomoto, Masa-aki Kawano, Hiroko Tsukamoto, Fumitaka Takeshita, Takeshi Imai, Takahiro Ochiya, Kohsuke Kataoka, Yuki Yamaguchi, Hiroshi Handa

    JOURNAL OF BIOTECHNOLOGY   135 ( 4 )   385 - 392   2008.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbiotec.2008.05.012

    Web of Science

    PubMed

    researchmap

  • Engineering of SV40-based nano-capsules for delivery of heterologous proteins as fusions with the minor capsid proteins VP2/3 Reviewed

    Takarnasa Inoue, Masa-Aki Kawano, Ryou-U Takahashi, Hiroko Tsukamoto, Teruya Enomoto, Takeshi Imai, Kohsuke Kataoka, Hiroshi Handa

    JOURNAL OF BIOTECHNOLOGY   134 ( 1-2 )   181 - 192   2008.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbiotec.2007.12.006

    Web of Science

    PubMed

    researchmap

  • Atirazine binds to F1F0-ATP synthase and inhibits mitochondrial function in sperm Reviewed

    Yasuyoshi Hase, Michiko Tatsuno, Takeyuki Nishi, Kosuke Kataoka, Yasuaki Kabe, Yuki Yamaguchi, Nobuaki Ozawa, Michiya Natori, Hiroshi Handa, Hajime Watanabe

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   366 ( 1 )   66 - 72   2008.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2007.11.107

    Web of Science

    PubMed

    researchmap

  • Evidence that SV40VP1-DNA interactions contribute to the assembly of 40-nm spherical viral particles Reviewed

    Hiroko Tsukamoto, Masa-aki Kawano, Takamasa Inoue, Teruya Enomoto, Ryou-u Takahashi, Naoki Yokoyama, Noriaki Yamamoto, Takeshi Imai, Kohsuke Kataoka, Yuki Yamaguchi, Hiroshi Handa

    GENES TO CELLS   12 ( 11 )   1267 - 1279   2007.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1365-2443.2007.01134.x

    Web of Science

    PubMed

    researchmap

  • MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3 Reviewed

    Song-iee Han, Shinsaku Aramata, Kunio Yasuda, Kohsuke Kataoka

    MOLECULAR AND CELLULAR BIOLOGY   27 ( 19 )   6593 - 6605   2007.10

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/MCB.01573-06

    Web of Science

    PubMed

    researchmap

  • L-Maf regulates p27kip1 expression during chick lens fiber differentiation Reviewed

    Hasan Mahmud Reza, Hirofumi Nishi, Kohsuke Kataoka, Yoshiko Takahashi, Kunio Yasuda

    DIFFERENTIATION   75 ( 8 )   737 - 744   2007.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1432-0436.2007.00171.x

    Web of Science

    PubMed

    researchmap

  • Multiple mechanisms and functions of Maf transcription factors in the regulation of tissue-specific genes Reviewed

    Kohsuke Kataoka

    JOURNAL OF BIOCHEMISTRY   141 ( 6 )   775 - 781   2007.6

     More details

    Authorship:Lead author, Corresponding author   Language:English  

    DOI: 10.1093/jb/mvm105

    Web of Science

    PubMed

    researchmap

  • MAFA controls genes implicated in insulin biosynthesis and secretion Reviewed

    H. Wang, T. Brun, K. Kataoka, A. J. Sharma, C. B. Wollheim

    DIABETOLOGIA   50 ( 2 )   348 - 358   2007.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00125-006-0490-2

    Web of Science

    PubMed

    researchmap

  • Roles and regulation of transcription factor MafA in islet β-cells Reviewed

    Shinsaku Aramata, Song-Iee Han, Kohsuke Kataoka

    Endocrine Journal   54 ( 5 )   659 - 666   2007

     More details

    Authorship:Corresponding author   Language:English   Publisher:5  

    DOI: 10.1507/endocrj.KR-101

    Scopus

    PubMed

    researchmap

  • The VP2/VP3 minor capsid protein of simian virus 40 promotes the in vitro assembly of the major capsid protein VP1 into particles Reviewed

    M Kawano, T Inoue, H Tsukamoto, T Takaya, T Enomoto, R Takahashi, N Yokoyama, N Yamamoto, A Nakanishi, T Imai, T Wada, K Kataoka, H Handa

    JOURNAL OF BIOLOGICAL CHEMISTRY   281 ( 15 )   10164 - 10173   2006.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M511261200

    Web of Science

    PubMed

    researchmap

  • Synergistic activation of the insulin gene promoter by the beta-cell enriched transcription factors MafA, Beta2, and Pdx1 Reviewed

    S Aramata, S Han, K Yasuda, K Kataoka

    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION   1730 ( 1 )   41 - 46   2005.7

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbaexp.2005.05.009

    Web of Science

    researchmap

  • Selective ligand purification using high-performance affinity beads Reviewed

    Y Ohtsu, R Ohba, Y Imamura, M Kobayashi, H Hatori, T Zenkoh, M Hatakeyama, T Manabe, M Hino, Y Yamaguchi, K Kataoka, H Kawaguchi, H Watanabe, H Handa

    ANALYTICAL BIOCHEMISTRY   338 ( 2 )   245 - 252   2005.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ab.2004.10.006

    Web of Science

    PubMed

    researchmap

  • Rep68 protein of adeno-associated virus type 2 interacts with 14-3-3 proteins depending on phosphorylation at serine 535 Reviewed

    SI Han, MA Kawano, KI Ishizu, H Watanabe, M Hasegawa, SN Kanesashi, YS Kim, A Nakanishi, K Kataoka, H Handa

    VIROLOGY   320 ( 1 )   144 - 155   2004.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.virol.2003.11.024

    Web of Science

    PubMed

    researchmap

  • Differentially expressed Maf family transcription factors, c-Maf and MafA, activate glucagon and insulin gene expression in pancreatic islet alpha- and beta-cells Reviewed

    K Kataoka, S Shioda, K Ando, K Sakagami, H Handa, K Yasuda

    JOURNAL OF MOLECULAR ENDOCRINOLOGY   32 ( 1 )   9 - 20   2004.2

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1677/jme.0.0320009

    Web of Science

    PubMed

    researchmap

  • Isolation and characterization of an alternatively spliced variant of transcription factor Islet-1 Reviewed

    K Ando, S Shioda, H Handa, K Kataoka

    JOURNAL OF MOLECULAR ENDOCRINOLOGY   31 ( 3 )   419 - 425   2003.12

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1677/jme.0.0310419

    Web of Science

    PubMed

    researchmap

  • Artificial oncoproteins: modified versions of the yeast bZip protein GCN4 induce cellular transformation Reviewed

    M Nishizawa, SL Fu, K Kataoka, PK Vogt

    ONCOGENE   22 ( 39 )   7931 - 7941   2003.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/sj.onc.1206527

    Web of Science

    PubMed

    researchmap

  • MafA has strong cell transforming ability but is a weak transactivator Reviewed

    M Nishizawa, K Kataoka, PK Vogt

    ONCOGENE   22 ( 39 )   7882 - 7890   2003.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/sj.onc.1206526

    Web of Science

    PubMed

    researchmap

  • Simian virus 40 VP1 capsid protein forms polymorphic assemblies in vitro Reviewed

    SN Kanesashi, K Ishizu, M Kawano, S Han, S Tomita, H Watanabe, K Kataoka, K Handa

    JOURNAL OF GENERAL VIROLOGY   84   1899 - 1905   2003.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1099/vir.0.19067-0

    Web of Science

    PubMed

    researchmap

  • MafA is a glucose-regulated and pancreatic beta-cell-specific transcriptional activator for the insulin gene Reviewed

    K Kataoka, SI Han, S Shioda, M Hirai, M Nishizawa, H Handa

    JOURNAL OF BIOLOGICAL CHEMISTRY   277 ( 51 )   49903 - 49910   2002.12

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M206796200

    Web of Science

    PubMed

    researchmap

  • Spatial redox regulation of a critical cysteine residue of NF-kappa B in vivo Reviewed

    T Nishi, N Shimizu, M Hiramoto, L Sato, Y Yamaguchi, M Hasegawa, S Aizawa, H Tanaka, K Kataoka, H Watanabe, H Handa

    JOURNAL OF BIOLOGICAL CHEMISTRY   277 ( 46 )   44548 - 44556   2002.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M202970200

    Web of Science

    PubMed

    researchmap

  • YEAF1/RYBP and YAF-2 are functionally distinct members of a cofactor family for the YY1 and E4TF1/hGABP transcription factors Reviewed

    C Sawa, T Yoshikawa, F Matsuda-Suzuki, S Delehouzee, M Goto, H Watanabe, J Sawada, K Kataoka, H Handa

    JOURNAL OF BIOLOGICAL CHEMISTRY   277 ( 25 )   22484 - 22490   2002.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M203060200

    Web of Science

    PubMed

    researchmap

  • Maf and Jun nuclear oncoproteins share downstream target genes for inducing cell transformation Reviewed

    K Kataoka, S Shioda, K Yoshitomo-Nakagawa, H Handa, M Nishizawa

    JOURNAL OF BIOLOGICAL CHEMISTRY   276 ( 39 )   36849 - 36856   2001.9

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M102234200

    Web of Science

    PubMed

    researchmap

  • Induction of cellular antioxidative stress genes through heterodimeric transcription factor Nrf2/small Maf by antirheumatic gold(I) compounds Reviewed

    K Kataoka, H Handa, M Nishizawa

    JOURNAL OF BIOLOGICAL CHEMISTRY   276 ( 36 )   34074 - 34081   2001.9

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M105383200

    Web of Science

    PubMed

    researchmap

  • Cloning of the hamster androgen receptor gene Reviewed

    K Shiba, T Hamaguchi, K Kataoka, Y Yamaguchi, H Handa, K Adachi

    JOURNAL OF DERMATOLOGICAL SCIENCE   26 ( 3 )   163 - 168   2001.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0923-1811(00)00172-9

    Web of Science

    PubMed

    researchmap

  • Roles of disulfide linkage and calcium ion-mediated interactions in assembly and disassembly of virus-like particles composed of simian virus 40 VP1 capsid protein Reviewed

    KI Ishizu, H Watanabe, SI Han, SN Kanesashi, M Hoque, H Yajima, K Kataoka, H Handa

    JOURNAL OF VIROLOGY   75 ( 1 )   61 - 72   2001.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/JVI.75.1.61-72.2001

    Web of Science

    PubMed

    researchmap

  • A set of Hox proteins interact with the Maf oncoprotein to inhibit its DNA binding, transactivation, and transforming activities Reviewed

    K Kataoka, K Yoshitomo-Nakagawa, S Shioda, M Nishizawa

    JOURNAL OF BIOLOGICAL CHEMISTRY   276 ( 1 )   819 - 826   2001.1

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M007643200

    Web of Science

    PubMed

    researchmap

  • Transactivation activity of Maf nuclear oncoprotein is modulated by Jun, Fos and small Maf proteins Reviewed

    K Kataoka, M Noda, M Nishizawa

    ONCOGENE   12 ( 1 )   53 - 62   1996.1

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    PubMed

    researchmap

  • SMALL MAF PROTEINS HETERODIMERIZE WITH FOS AND MAY ACT AS COMPETITIVE REPRESSORS OF THE NF-E2 TRANSCRIPTION FACTOR Reviewed

    K KATAOKA, K IGARASHI, K ITOH, KT FUJIWARA, M NODA, M YAMAMOTO, M NISHIZAWA

    MOLECULAR AND CELLULAR BIOLOGY   15 ( 4 )   2180 - 2190   1995.4

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    PubMed

    researchmap

  • MAFB, A NEW MAF FAMILY TRANSCRIPTION ACTIVATOR THAT CAN ASSOCIATE WITH MAF AND FOS BUT NOT WITH JUN Reviewed

    K KATAOKA, KT FUJIWARA, M NODA, M NISHIZAWA

    MOLECULAR AND CELLULAR BIOLOGY   14 ( 11 )   7581 - 7591   1994.11

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    PubMed

    researchmap

  • [Maf nuclear oncoprotein family members as transcriptional regulators]. Reviewed

    Nishizawa M, Kataoka K

    Seikagaku. The Journal of Japanese Biochemical Society   66 ( 10 )   1327 - 1331   1994.10

     More details

  • REGULATION OF TRANSCRIPTION BY DIMERIZATION OF ERYTHROID FACTOR NF-E2 P45 WITH SMALL MAF PROTEINS Reviewed

    K IGARASHI, K KATAOKA, K ITOH, N HAYASHI, M NISHIZAWA, M YAMAMOTO

    NATURE   367 ( 6463 )   568 - 572   1994.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/367568a0

    Web of Science

    PubMed

    researchmap

  • MAF NUCLEAR ONCOPROTEIN RECOGNIZES SEQUENCES RELATED TO AN AP-1 SITE AND FORMS HETERODIMERS WITH BOTH FOS AND JUN Reviewed

    K KATAOKA, M NODA, M NISHIZAWA

    MOLECULAR AND CELLULAR BIOLOGY   14 ( 1 )   700 - 712   1994.1

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    PubMed

    researchmap

  • 2 NEW MEMBERS OF THE MAF ONCOGENE FAMILY, MAFK AND MAFF, ENCODE NUCLEAR B-ZIP PROTEINS LACKING PUTATIVE TRANSACTIVATOR DOMAIN Reviewed

    KT FUJIWARA, K KATAOKA, M NISHIZAWA

    ONCOGENE   8 ( 9 )   2371 - 2380   1993.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    researchmap

  • STRUCTURE-FUNCTION ANALYSIS OF THE MAF ONCOGENE PRODUCT, A MEMBER OF THE B-ZIP PROTEIN FAMILY Reviewed

    K KATAOKA, M NISHIZAWA, S KAWAI

    JOURNAL OF VIROLOGY   67 ( 4 )   2133 - 2141   1993.4

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    PubMed

    researchmap

  • ISOLATION OF THE AVIAN TRANSFORMING RETROVIRUS, AS42, CARRYING THE V-MAF ONCOGENE AND INITIAL CHARACTERIZATION OF ITS GENE-PRODUCT Reviewed

    S KAWAI, N GOTO, K KATAOKA, T SAEGUSA, H SHINNOKOHNO, M NISHIZAWA

    VIROLOGY   188 ( 2 )   778 - 784   1992.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/0042-6822(92)90532-T

    Web of Science

    PubMed

    researchmap

  • LOCALIZATION OF THE HUMAN MAF PROTOONCOGENE ON CHROMOSOME-16 TO BANDS Q22-Q23 Reviewed

    MC YOSHIDA, M NISHIZAWA, K KATAOKA, N GOTO, KT FUJIWARA, S KAWAI

    CYTOGENETICS AND CELL GENETICS   58 ( 3-4 )   2003 - 2003   1991

     More details

    Language:English  

    Web of Science

    researchmap

  • V-MAF, A VIRAL ONCOGENE THAT ENCODES A LEUCINE ZIPPER MOTIF - (AVIAN RETROVIRUS TRANSFORMATION DNA-BINDING PROTEIN) Reviewed

    M NISHIZAWA, K KATAOKA, N GOTO, KT FUJIWARA, S KAWAI

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   86 ( 20 )   7711 - 7715   1989.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.86.20.7711

    Web of Science

    PubMed

    researchmap

▼display all

MISC

  • 膵島Β細胞特異的転写因子MafAの多重リン酸化による細胞増殖制御機構

    金井賢一, 片岡浩介

    日本生化学会大会(Web)   88th   2P0760 (WEB ONLY)   2015

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 皮膚の天然保湿因子(NMF)の形成における転写因子MafBの役割

    片岡浩介

    コスメトロジー研究報告   22   90 - 94   2014.9

     More details

  • 発生過程の四肢においてAP‐1転写因子のコンビネーションが細胞死を制御する機構

    須田夏野, 白川大介, 伊藤武彦, 中戸隆一郎, 坂東優篤, 白髭克彦, 片岡浩介, TICKLE Cheryll, 田中幹子

    日本動物学会大会予稿集   84th   178   2013.8

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • インスリン転写調節因子MafAは胸腺におけるインスリン発現と1型糖尿病疾患感受性に関与する

    能宗伸輔, 片岡浩介, 川畑由美子, 馬場谷成, 廣峰義久, 藤澤智巳, 池上博司

    日本体質医学会雑誌   73 ( 1 )   18 - 23   2011.2

     More details

  • インスリン転写調節因子MafAは胸腺におけるインスリン発現と1型糖尿病疾患感受性に関与する

    能宗伸輔, 片岡浩介, 川畑由美子, 馬場谷成, 廣峰義久, 藤澤智巳, 池上博司

    日本体質医学会雑誌   72 ( 3 )   157   2010.9

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 肥満基礎研究の進歩 エネルギー代謝とその調節機序 エネルギー代謝にかかわる因子 転写因子 Maf

    片岡浩介

    日本臨床   68   195 - 199   2010.2

     More details

  • 転写因子MafAのトランスフォーメーション活性におけるリン酸化とSUMO化の役割

    金井賢一, 片岡浩介

    生化学   ROMBUNNO.3T20P-10   2009.9

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 除草剤アトラジンの精子運動低下作用解析

    梨本明宏, 羽瀬康嘉, 片岡浩介, 加部泰明, 小澤伸晃, 名取道也, 渡辺肇, 半田宏

    生化学   ROMBUNNO.4P-697   2009.9

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 膵β細胞の分化と機能維持を司る転写因子MafA

    片岡浩介

    分子糖尿病学の進歩   2009   2 - 7   2009.4

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • フタル酸エステルの生体内標的分子の同定

    渡邉肇, 倉森見典, 片岡浩介, 半田宏

    Endocrine Disrupter News Letter   11 ( 4 )   2   2009.3

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 表皮ケラチノサイト分化における転写ネットワークの解明

    宮井雅史, 神谷明代, 濱田理人, 森口尚, 高橋智, 片岡浩介

    生化学   3P-0674   2008

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Maf転写因子群の多重なリン酸化を介した複数の分解経路

    金井賢一, 片岡浩介

    生化学   4T10-1   2008

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 転写因子MafBによる副甲状腺の分化と移動の制御

    神谷明代, 濱田理人, 宮井雅史, 森口尚, 高橋智, 片岡浩介

    生化学   81回・31回   2T3-7 - 7   2008

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 転写因子SnailのPA28γによるタンパク質安定性制御

    片上紗世, 金井賢一, 片岡浩介

    生化学   3P-0660   2008

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 転写因子MafAの翻訳後修飾による活性の制御

    金井賢一, HASAN‐MAHMUD Reza, 神谷明代, 荒俣晋作, SONG‐IEE Han, 安田國雄, 片岡浩介

    生化学   1T3-11   2008

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 遺伝情報発現と疾患 2.Maf転写因子の生理機能と疾患・発癌

    片岡浩介

    実験医学   25 ( 10 )   1605 - 1611   2007.6

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 膵島β細胞に特異的な転写因子群の生化学―膵島細胞の再生医療に不可欠なステップ―

    片岡浩介

    医科学応用研究財団研究報告   24   183 - 185   2007.2

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 副甲状腺における転写因子MafBの役割

    神谷明代, 濱田理人, 森口尚, 高橋智, 片岡浩介

    生化学   2P-1045   2007

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 皮膚及び毛包におけるMaf転写因子の発現と機能.

    宮井 雅史, 田中 義啓, 濱田 理人, 森口 尚, 高橋 智, 片岡 浩介

    日本発生生物学会・日本細胞生物学会合同大会要旨集   40回・59回   96 - 96   2007

     More details

  • 膵島β細胞におけるグルコースに応答した転写因子MafA安定性制御機構の解析

    荒俣晋作, HAN Song‐Iee, 安田國雄, 片岡浩介

    生化学   2P-0950   2007

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 皮膚及び毛包における転写因子Mafの発現と機能

    宮井雅史, 田中義啓, 濱田理人, 森口尚, 高橋智, 片岡浩介

    生化学   2P-0956   2007

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 転写因子MafファミリーのSUMO化による機能修飾

    金井賢一, 荒俣晋作, 神谷明代, 片岡浩介

    生化学   2P-0954   2007

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Genetic information decoding system which controls cell differentiation: Transcription factor, chromatin control, non-coding RNA -- Insulin gene expression control and Langerhans’ islet beta cell differentiation program.

    片岡浩介

    細胞工学   25 ( 1 )   28 - 32   2005.12

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • すい島β細胞特異的なインスリン転写因子MafAのグルコース応答性の分子基盤

    HAN Song‐Iee, 荒俣晋作, 片岡浩介

    日本分子生物学会年会講演要旨集   28th   418   2005.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 転写因子MafAのリン酸化によるインスリン遺伝子発現調節機構の解析

    HAN Song‐Iee, 荒俣晋作, 神谷明代, 安田国雄, 片岡浩介

    日本分子生物学会年会講演要旨集   28th   441   2005.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • β細胞特異的な転写因子MafA,Beta2,Pdx1によるインスリン遺伝子の協調的な転写活性化

    荒俣晋作, HAN Song‐Iee, 安田国雄, 片岡浩介

    日本分子生物学会年会講演要旨集   28th   424   2005.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Regeneration and reparation of ocular tissue using stem cell and morphogenetic gene. Function of lens-specific transcription factor MafA/L-Maf.

    片岡浩介

    幹細胞と形態形成遺伝子を用いた眼組織の再生と修復に関する研究 平成15年度 総括・分担研究報告書   19   2004

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Development of chromosomal translocation specifically observed in multiple myeloma from molecular biology to biochemistry.

    片岡浩介

    がん研究に係わる特定領域研究研究報告集録 平成15年度(CD-ROM)   HATSUGANTOBOUGYO.125-HATSUGANTOBOUGYO.126   2004

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • bZip転写因子MafAはグルコース濃度依存的/すいbeta細胞特異的なインスリン発現制御因子である

    片岡浩介, 韓松伊, 西沢誠, 半田宏

    生化学   74 ( 8 )   914   2002.8

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 4 degrees C preparation of ferrite nanoparticles having protein molecules immobilized on their surfaces

    K Nishimura, M Hasegawa, Y Ogura, T Nishi, K Kataoka, H Handa, M Abe

    JOURNAL OF APPLIED PHYSICS   91 ( 10 )   8555 - 8556   2002.5

     More details

  • レドックス制御による転写因子NF‐κBの活性化機構

    西剛志, 清水宣明, TANG J, 佐藤巌, 片岡浩介, 渡辺肇, 平本正樹, 半田宏

    日本分子生物学会年会プログラム・講演要旨集   23rd   528   2000.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 抗リウマチ金製剤の作用機序 bZip型転写因子Nrf2/Small Mafの活性化

    片岡浩介, 中川(吉友)清美, 塩田節子, 半田宏, 西沢誠

    日本分子生物学会年会プログラム・講演要旨集   22nd   692   1999.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • MafおよびJun癌遺伝子産物による転写活性化と細胞のトランスフォーメーション

    中川(吉友)清美, 塩田節子, 西沢誠, 片岡浩介

    日本癌学会総会記事   58th   219   1999.8

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • 重金属イオンによるMARE関連cis‐DNA配列の転写活性化

    片岡浩介, 塩田節子, 中川(吉友)清美, 西沢誠

    日本癌学会総会記事   58th   483   1999.8

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Ma転写因子ファミリーによる転写調節と細胞のトランスフォーメーション

    片岡浩介, 中川(吉友)清美, 塩田節子, 西沢誠

    日本分子生物学会年会プログラム・講演要旨集   21st   563   1998.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Maf転写因子ファミリーと相互作用する蛋白質の検索

    中川(吉友)清美, 塩田節子, 西沢誠, 片岡浩介

    日本分子生物学会年会プログラム・講演要旨集   21st   516   1998.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Isolation of New Maf Oncogene Product‐binding Proteins.

    片岡浩介, 中川(吉友)清美, 塩田節子, 西沢誠

    日本分子生物学会年会プログラム・講演要旨集   20th   399   1997.12

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Oncogenes in Development and Cell Differentiation. Regulation of Cell Growth and Differentiation by maf Oncogene Family.

    片岡浩介, 西沢誠

    最新医学   51 ( 11 )   2163 - 2168   1996.11

     More details

  • Family and super family of genes and proteins. Expression/transfer of gene. Maf family.

    片岡浩介, 西沢誠

    生体の科学   46 ( 5 )   409 - 410   1995.9

     More details

  • Transcription of a cell-surface antigen gene expressed on SV40-transformed cells.

    DUCKHEE H, 栗原利和, 塩田節子, 丸山和夫, 片岡浩介, 山口宣生

    日本分子生物学会年会プログラム・講演要旨集   17th   370   1994.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Transcriptional regulatory function of Maf family proteins.

    西沢誠, 片岡浩介

    日本分子生物学会年会プログラム・講演要旨集   17th   333   1994.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Maf nuclear oncoprotein family members as transcriptional regulators.

    西沢誠, 片岡浩介

    生化学   66 ( 10 )   1327 - 1331   1994.10

     More details

  • maf関連遺伝子群による赤血球特異的転写因子NF‐E2の機能変換

    五十嵐和彦, 伊東健, 山本雅之, 片岡浩介, 西沢誠

    日本分子生物学会年会プログラム・講演要旨集   16th   270   1993.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • トリ赤血球特異的転写因子NF‐E2の構造解析

    伊東健, 五十嵐和彦, 山本雅之, 片岡浩介, 西沢誠

    日本分子生物学会年会プログラム・講演要旨集   16th   269   1993.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • GATAおよびNF‐E2転写因子と赤血球分化

    山本雅之, 五十嵐和彦, 石原元, 伊藤健, 蓬田健太郎, 片岡浩介, 西沢誠

    日本分子生物学会年会プログラム・講演要旨集   16th   154   1993.11

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • maf oncogene and its related genes, which encode leucine zipper motif.

    西沢誠, 片岡浩介

    実験医学   11 ( 8 )   1053 - 1058   1993.5

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

▼display all

Research Projects

  • Molecular mechanism underlying secondary hyperparathyroidism in chronic kidney disease

    Grant number:21K08561  2021.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

      More details

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    researchmap

  • Breakdown of MafA regulatory system leading to b-cell failure during type 2 diabetes progression

    Grant number:16K09763  2016.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Kataoka Kohsuke

      More details

    Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

    During progression of type 2 diabetes, pancreatic islet b-cells gradually lose their ability to secrete insulin in response to glucose. Loss or decrease of MafA transcription factor has been thought be responsible for the b-cell dysfunction. Here, we identified a intracellular system regulating MafA protein function and stability.
    We found that an amino acid-related compound Taurine activates WNK kinase in b-cells, thereby maintains MafA protein levels. We also found that basal level autophagy is required for maintaining mafA mRNA transcription. Furthermore, glucocorticoid and related compounds inhibit ability of MafA-Beta2-HNF1b transcription factors to synergistically activate glut2 gene transcription. As Glut2 (glucose transporter 2) is an essential component of glucose-stimulated insulin secretion in b-cells, this mechanism may explain how steroids suppress b-cell function.

    researchmap

  • 血糖値に応答してインスリン遺伝子の転写を行う膵島β細胞特異的転写因子MafAの生化学的機構の解明

    Grant number:04F04189  2004 - 2005

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    片岡 浩介, HAN Song-iee

      More details

    Grant amount:\2400000 ( Direct Cost: \2400000 )

    インスリンは血糖値を保つために必要不可欠なペプチドホルモンであり、その制御システムの破綻は糖尿病を引き起こす。インスリン遺伝子の発現は膵臓ランゲルハンス島のβ細胞に特異的で、かつグルコース濃度依存的であり、bZip型転写因子MafAがこれを担う重要な因子である。本研究は、MafAの活性の制御機構を分子生物学的・生化学的手法を用いて解析することにより、これまで未解決であったインスリン遺伝子のグルコースによる発現調節を解明することを目的とする。
    前年度までに、膵島β細胞由来細胞株におけるMafAタンパク質の翻訳後修飾とその意義を解析した。本年度はさらに詳細な解析を継続して行い、膵島β細胞でのインスリン遺伝子発現のグルコース応答におけるMafAの役割を分子レベルであきらかにすることができた(投稿準備中)。
    また、その研究の途上で、MafAタンパク質が機能を発揮するために必要なコファクターの存在を発見し、同定することに成功した。すなわち、MafAタンパク質はホモ2量体としてDNAに結合して遺伝子発現の制御を行っていると考えられてきたが、β細胞内では別の転写制御因子とヘテロ2量体を形成していることを発見した。さらにこの因子の実体を、候補と考えられる転写因子に対する特異抗体を各種用いて検討することにより同定した。この因子のcDNAを入手し、そのDominant Negative型変異体を作製して膵島β細胞株に導入したり、その発現をsiRNA法により抑制したりすることによる機能阻害実験を行ったところ、この因子がインスリン遺伝子プロモーターの活性制御において重要な役割を担っていることをあきらかにすることができた(投稿準備中)。以上の結果は、インスリン遺伝子発現制御や膵島β細胞の発生・分化における未知の制御機構が存在することを明確に示すものであり、今後、MafAおよびこの因子が、血糖値の維持や糖尿病の発症にどのように関わっているのかをより詳細に追究する必要がある。

    researchmap

  • 合成DNAマイクロアレイによる病態関連遺伝子発現解析法の開発

    Grant number:12204044  2000

    日本学術振興会  科学研究費助成事業 特定領域研究(C)  特定領域研究(C)

    渡辺 慎哉, 片岡 浩介

      More details

    平成12年度は、まずオリゴDNAを市販のスライドガラス上に効率良く固相化する方法を開発した。次に、均質なマイクロアレイを一度に84枚作製できるマイクロアレイ作製装置およびプログラムソフトウエアを開発した。さらに、2708ヒト遺伝子について80merのヌクレオチドを設計・合成・アレイ化し、ヒトサイトメガロウイルスを対象としてウイルス感染に伴って変化する宿主遺伝子発現変化を解析し、今年度の目標である系の確立を確認した。
    以上の結果をもととして、合成DNAマイクロアレイ技術に関する以下の特許申請を行った。
    A:発明の名称:試料チップ作製用分注針の洗浄方法
    願書番号:特願2000-53109号
    B:発明の名称:ポリヌタレオチドマイクロアレイの作製方法、作製装置ならびにポリヌクレオチドマイクロアレイ
    願書番号:特願2000-139926号
    C:発明の名称:試料チップ作製方法
    願書番号:特願2000-147606号
    D:発明の名称:核酸標識方法および核酸標識用キット
    願書番号:特願2000-254172号
    ウイルス感染に伴う細胞応答のトランスクリプトーム解析に関しては、スタンフォード大学などの創始グループが最大約8,000遺伝子について報告しており、今後、さらに対象遺伝子の数を大きくし、大量の情報を得るべく国内外を含めて凌ぎを削っている状況にある。マイクロアレイの技術そのものがいまだに発展途上にあるため、技術的な問題をいかに早く克服して質・量ともに優れたデータを集積できるかどうかに成功の鍵があるといえる。本研究は独自のマイクロアレイ技術に基盤を置くもので、量産へむけての障害をほぼ克服済みであり、今後、先行するパイオニア等に十分伍していけるものと考える。

    researchmap

  • DNA腫瘍ウイルス蛋白による転写の変換

    Grant number:08264105  1996 - 1998

    日本学術振興会  科学研究費助成事業 特定領域研究(A)  特定領域研究(A)

    半田 宏, 池田 正明, 片岡 浩介, 横山 一成, 澤田 幸治, 石井 俊輔

      More details

    Grant amount:\110000000 ( Direct Cost: \110000000 )

    DNA腫瘍ウイルスのがんタンパク質による転写脱制御による細胞がん化機構を解明することを目的として、がんウイルスタンパク質と相互作用する細胞性転写因子やコアクチベータ-などの機能を分子・細胞・個体レベルで解析し、がんウイルスタンパク質に関連する新規転写因子やコファクターの構造・機能解析を行った。研究成果を以下に挙げる。(1)転写伸長反応を制御する新規転写因子DSFを同定・精製し、そのcDNAクローン化した。転写因子ATFとTBPとの間のコアクチベータ-hMBFlのcDNAクローンを単離した(半田)。(2)コアクチベータCBPの機能をハエやマウスの個体レベルで解析した。ハエホモローグdCBPは、転写因子DorsalやCiのコアクチベータ-として働き、形態形成に重要な役割を果たすことを見つけた。また、CBP欠損ホモマウスは胎生致死で種々の骨形成異常を来すことを発見した(石井)。(3)強発癌性と弱発癌性ElAタンパク質の間では、リン酸化による構造・機能変化およおびp300との結合に関わる主要部位が異なることを見つけた(澤田)。(4)レチノイン酸によるP9細胞の分化に関与するCBPとp300は、機能分担すていることを明らかにした(横田)。(5)転写因子Mafによる転写活性化はトランスフォーメーションに必須で、それはElAにより抑制されることを見つけた。Maf-のbZip領域に結合するホメオボックスを持つ二種のコファクターcDNAを単離した(片岡)。(6)最終分化した心筋細胞では、成長因子などの刺激により心肥大を引き起こす。この過程で、Glサイクリンcdkは活性化されるが、その標的がRb・E2F以外であることを見いだした(池田)。(7)グルココルチコイド受容体(GR)とAP-lの両者はCBPを奪い合うことにより互いの転写活性を阻害すると従来考えられていた。しかし、CBPとの結合能を欠損したGR変異体でもAP-lによるトランスフォーメーションを阻害し、一方、DNA結合能を欠いた変異体は阻害しないことから、従来の説とは異なりbZp構造がこの阻害反応に関与することを見つけた(伊庭)。(8)ElAと相同性のあるHSlの構造・機能解析を行い、HSlが抗原刺激核により核内に移行することを見いだした。核内に移行する機序を現在解析している解析(北村)。

    researchmap