担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/md22060269

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.52794/hujpharm.1354419

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

A Cucurbita phloem exudate lectin (CPL) from summer squash (Cucurbita pepo) fruits was isolated and its sugar-binding properties and biological activities were studied. The lectin was purified by affinity chromatography and the hemagglutination assay method was used to determine its pH, heat stability, metal-dependency and sugar specificity. Antimicrobial and anticancer activities were also studied by disc diffusion assays and in vivo and in vitro methods. The molecular weight of CPL was 30 ± 1 KDa and it was stable at different pH (5.0 to 9.0) and temperatures (30 to 60 °C). CPL recovered its hemagglutination activity in the presence of Ca2+. 4-nitrophenyl-α-D-glucopyranoside, lactose, rhamnose and N-acetyl-D-glucosamine strongly inhibited the activity. With an LC50 value of 265 µg/mL, CPL was moderately toxic and exhibited bacteriostatic, bactericidal and antibiofilm activities against different pathogenic bacteria. It also exhibited marked antifungal activity against Aspergillus niger and agglutinated A. flavus spores. In vivo antiproliferative activity against Ehrlich ascites carcinoma (EAC) cells in Swiss albino mice was observed when CPL exerted 36.44% and 66.66% growth inhibition at doses of 3.0 mg/kg/day and 6.0 mg/kg/day, respectively. A 12-day treatment by CPL could reverse their RBC and WBC counts as well as restore the hemoglobin percentage to normal levels. The MTT assay of CPL performed against human breast (MCF-7) and lung (A-549) cancer cell lines showed 29.53% and 18.30% of inhibitory activity at concentrations of 128 and 256 µg/mL, respectively.

DOI： 10.3390/molecules29112531

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

This research describes the synthesis by an environmentally-friendly method, microwave irradiation, development and analysis of three novel and one previously identified Schiff base derivative as a potential inhibitor of bovine xanthine oxidase (BXO), a key enzyme implicated in the progression of gout. Meticulous experimentation revealed that these compounds (10, 9, 4, and 7) have noteworthy inhibitory effects on BXO, with IC50 values ranging from 149.56 µM to 263.60 µM, indicating their good efficacy compared to that of the standard control. The validation of these results was further enhanced through comprehensive in silico studies, which revealed the pivotal interactions between the inhibitors and the catalytic sites of BXO, with a particular emphasis on the imine group (-C = N-) functionalities. Intriguingly, the compounds exhibiting the highest inhibition rates also showcase advantageous ADMET profiles, alongside encouraging initial assessments via PASS, hinting at their broad-spectrum potential. The implications of these findings are profound, suggesting that these Schiff base derivatives not only offer a new vantage point for the inhibition of BXO but also hold considerable promise as innovative therapeutic agents in the management and treatment of gout, marking a significant leap forward in the quest for more effective gout interventions.

DOI： 10.1016/j.jsps.2024.102062

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担当区分：最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Nucleoside analogs have been widely used as antiviral, antitumor, and antiparasitic agents due to their ability to inhibit nucleic acid synthesis. Adenosine, cytidine, guanosine, thymidine and uridine analogs such as didanosine, vidarabine, remdesivir, gemcitabine, lamivudine, acyclovir, abacavir, zidovusine, stavudine, and idoxuridine showed remarkable anticancer and antiviral activities. In our previously published articles, our main intention was to develop newer generation nucleoside analogs with acylation-induced modification of the hydroxyl group and showcase their biological potencies. In the process of developing nucleoside analogs, in silico studies play an important role and provide a scientific background for biological data. Molecular interactions between drugs and receptors followed by assessment of their stability in physiological environments, help to optimize the drug development process and minimize the burden of unwanted synthesis. Computational approaches, such as DFT, FMO, MEP, ADMET prediction, PASS prediction, POM analysis, molecular docking, and molecular dynamics simulation, are the most popular tools to culminate all preclinical study data and deliver a molecule with maximum bioactivity and minimum toxicity. Although clinical drug trials are crucial for providing dosage recommendations, they can only indirectly provide mechanistic information through researchers for pathological, physiological, and pharmacological determinants. As a result, in silico approaches are increasingly used in drug discovery and development to provide mechanistic information of clinical value. This article portrays the current status of these methods and highlights some remarkable contributions to the development of nucleoside analogs with optimized bioactivity.

DOI： 10.2174/0113895575258033231024073521

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

DOI： 10.1016/j.ijbiomac.2023.127628

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

DOI： 10.1016/j.jsps.2023.101804

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担当区分：最終著者   掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/md21120614

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担当区分：最終著者   記述言語：英語  

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担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Association of Carbohydrate Chemists and Technologists, India (ACCTI www.accti.in)  

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その他リンク： https://www.trendscarbo.com/aboutjournal.php

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/molecules28010219

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/md20100613

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担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

PubMed

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担当区分：最終著者   記述言語：英語   出版者・発行元：Cold Spring Harbor Laboratory Press  

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その他リンク： https://www.ncbi.nlm.nih.gov/books/NBK579918/toc/?report=reader

記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Carbohydrate esters are significant in medicinal chemistry because of their efficacy for the synthesis of biologically active drugs. In the present study, methyl β-D-galactopyranoside (MGP) was treated with various acyl halides to produce 6-O-acyl MGP esters by direct acylation method with an excellent yield. To obtain newer products for antimicrobial assessment studies, the 6-O-MGP esters were further modified into 2,3,4-tri-O-acyl MGP esters containing a wide variety of functionalities in a single molecular framework. The chemical structures of the newly synthesized compounds were elucidated by analyzing their physicochemical, elemental, and spectroscopic data. In vitro antimicrobial testing against five bacteria and two fungi and the prediction of activity spectra for substances (PASS) revealed that these MGP estes have promising antifungal functionality compared to their antibacterial activities. The antimicrobial tests demonstrated that the compounds 3 and 10 were the most potent against Bacillus subtilis and Escherichia coli strains, with the minimum inhibitory concentration (MIC) values ranging from 0.352 ± 0.02 to 0.703 ± 0.01 mg/ml and minimum bactericidal concentration (MBC) values ranging from 0.704 ± 0.02 to 1.408 ± 0.04 mg/ml. Density functional theory (DFT) at the B3LYP/3-21G level of theory was employed to enumerate, frontier orbital energy, enthalpy, free energy, electronic energy, MEP, dipole moment which evaluated the effect of certain groups (aliphatic and aromatic) on drug properties. They discovered that all esters were more thermodynamically stable than the parent molecule. Molecular docking is performed using AutoDock Vina to determine the binding affinities and interactions between the MGP esters and the SARS-CoV-2 main protease. The modified esters strongly interact with the prime Cys145, His41, MET165, GLY143, THR26, and ASN142 residues. The MGP esters' shape and ability to form multiple electrostatic and hydrogen bonds with the active site match other minor-groove binders' binding modes. The molecular dynamics simulation validates the molecular docking results. The pharmacokinetic characterization of the optimized inhibitor demonstrates that these MGP esters appear to be safer inhibitors and a combination of in silico ADMET (absorption, distribution, metabolism, excretion, and toxicity) prediction and drug-likeness had promising results due to their improved kinetic properties. Structure activity relationships (SAR) study including in vitro and silico results revealed that the acyl chain, palmitoyl (C16) and 4-chlorobenzoyl (4.ClC₆H₄CO-) in combination with sugar were found the most potential activates against human and fungal pathogens. After all, our comprehensive computational and statistical analysis shows that these selected MGP esters can be used as potential inhibitors against the SARS-CoV-2.

DOI： 10.1007/s10719-021-10039-3

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MDPI AG  

DOI： 10.3390/molecules26227016

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MDPI  

DOI： 10.3390/molecules26164799

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/md19070394

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.29356/jmcs.v65i2.1464

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掲載種別：研究論文（学術雑誌）   出版者・発行元：Pensoft Publishers  

Nucleoside derivatives are important therapeutic drugs and are the focal point in the ongoing search for novel, more potent drug targets. In this study, a new series of pyrimidine nucleoside i.e., uridine (<bold>1</bold>) derivatives were synthesized via direct method and evaluated for their antimicrobial potential activity. The title compound uridine (<bold>1</bold>) was treated with triphenylmethyl chloride in pyridine to give the 5´-<italic>O</italic>-(triphenylmethyl)uridine derivative (<bold>2</bold>), which was subsequently derivatized to create a series of 2´,3´-di-<italic>O</italic>-acyl analogs containing a wide variety of functionalities in a single molecular framework. <italic>In vitro</italic> antimicrobial functionality tests were determined against both human and plant pathogens by disc diffusion and food poisoned techniques. The chemical structures of the synthesized compounds were confirmed on the basis of their spectral, analytical, physicochemical data. The antimicrobial results indicated that the synthesized derivatives exhibited moderate to good antibacterial and antifungal activity; in particular, they were found to be more effective against fungal phytopathogens than against human bacterial strains. Compounds <bold>7</bold>, <bold>9</bold>, and <bold>14</bold> were of particular interest as they exhibited noteworthy antifungal and antibacterial properties. <italic>In vitro</italic> MTT assays revealed that compound <bold>9</bold> was effective against Ehrlich’s ascites carcinoma (EAC) cells, resulting in 7.12% and 1.34% cell growth inhibition at concentrations of 200 and 6.25 µg/ml, respectively. The IC₅₀ value for compound <bold>9</bold> was rather high and found to be 1956.25 µg/ml. Structure-activity relationship (SAR) studies were also conducted to predict structural and pharmacokinetic properties. The findings of this study indicate that the different uridine derivatives are potentially useful antimicrobial agents for the advancement of future pharmaceutical research.

DOI： 10.3897/pharmacia.68.e56543

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その他リンク： https://pharmacia.pensoft.net/article/56543/download/xml/

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.2478/auoc-2021-0002

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Nucleoside analogs contribute in pharmaceutical and clinical fields as medicinal agents and approved drugs. This work focused to investigate the antimicrobial, anticancer activities, and structure-activity relationship (SAR) of cytidine and its analogs with computational studies. Microdilution was used to determine the antimicrobial activity, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of the modified analogs against human and phytopathogenic strains. Compounds (7), (10), and (14) were the most potent against Escherichia coli and Salmonella abony strains with MIC and MBC values from 0.316 ± 0.02 to 2.50 ± 0.03 and 0.625 ± 0.04 to 5.01 ± 0.06 mg/ml, respectively. The highest inhibitory activity was observed against gram-positive bacteria. Numerous analogs (10), (13), (14), and (15) exhibited good activity against the tested fungi Aspergillus niger and Aspergillus flavus. Anticancer activity of the cytidine analogs was examined through MTT colorimetric assay against Ehrlich's ascites carcinoma (EAC) tumor cells whereas compound 6 showed the maximum antiproliferative activity with an IC50 value of 1168.97 µg/ml. To rationalize this observation, their quantum mechanical and molecular docking studies have been performed against urate oxidase of A. flavus 1R51 to investigate the binding mode, binding affinity, and non-bonding interactions. It was observed that most of the analogs exhibited better binding properties than the parent drug. In silico ADMET prediction was attained to evaluate the drug-likeness properties that revealed the improved pharmacokinetic profile with lower acute oral toxicity of cytidine analogs. Based on the in vitro and in silico analysis, this exploration can be useful to develop promising cytidine-based antimicrobial drug(s). Supplementary Information: The online version contains supplementary material available at 10.1007/s40203-021-00102-0.

DOI： 10.1007/s40203-021-00102-0

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Nature Ltd  

添付ファイル： Kamata et al (2020) Sci Rep 10_22102.pdf

DOI： 10.1038/s41598-020-78926-7

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出版者・発行元：Book Publisher International (a part of {SCIENCEDOMAIN} International)  

DOI： 10.9734/bpi/cpcs/v3

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Sugawara et al (2020) Glycobiology.pdf

DOI： 10.1093/glycob/cwaa029

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その他リンク： http://academic.oup.com/glycob/article-pdf/30/10/802/33807038/cwaa029.pdf

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley  

A 15-kDa lectin, termed SeviL, was isolated from Mytilisepta virgata (purplish bifurcate mussel). SeviL forms a noncovalent dimer that binds strongly to ganglio-series GM1b oligosaccharide (Neu5Acɑ2-3Galβ1-3GalNAcβ1-4Galβ1-4Glc) and its precursor, asialo-GM1 (Galβ1-3GalNAcβ1-4Galβ1-4Glc). SeviL also interacts weakly with the glycan moiety of SSEA-4 hexaose (Neu5Acα2-3Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glc). A partial protein sequence of the lectin was determined by mass spectrometry, and the complete sequence was identified from transcriptomic analysis. SeviL, consisting of 129 amino acids, was classified as an R(icin B)-type lectin, based on the presence of the QxW motif characteristic of this fold. SeviL mRNA is highly expressed in gills and, in particular, mantle rim tissues. Orthologue sequences were identified in other species of the family Mytilidae, including Mytilus galloprovincialis, from which lectin MytiLec-1 was isolated and characterized in our previous studies. Thus, mytilid species contain lectins belonging to at least two distinct families (R-type lectins and mytilectins) that have a common β-trefoil fold structure but differing glycan-binding specificities. SeviL displayed notable cytotoxic (apoptotic) effects against various cultured cell lines (human breast, ovarian, and colonic cancer; dog kidney) that possess asialo-GM1 oligosaccharide at the cell surface. This cytotoxic effect was inhibited by the presence of anti-asialo-GM1 oligosaccharide antibodies. With HeLa ovarian cancer cells, SeviL showed dose- and time-dependent activation of kinase MKK3/6, p38 MAPK, and caspase-3/9. The transduction pathways activated by SeviL via the glycosphingolipid oligosaccharide were triggered apoptosis. DATABASE: Nucleotide sequence data have been deposited in the GenBank database under accession numbers MK434191, MK434192, MK434193, MK434194, MK434195, MK434196, MK434197, MK434198, MK434199, MK434200, and MK434201.

添付ファイル： Fuii et al (2020) FEBS J 287 2612-2630.pdf

DOI： 10.1111/febs.15154

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その他リンク： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/febs.15154

担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：論文集(書籍)内論文   出版者・発行元：Springer US  

DOI： 10.1007/978-1-0716-0430-4_21

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.25177/jccmm.4.4.ra.10663

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出版者・発行元：MDPI  

DOI： 10.3390/books978-3-03921-821-9

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MDPI AG  

MytiLec-1, a 17 kDa lectin with β-trefoil folding that was isolated from the Mediterranean mussel (Mytilus galloprovincialis) bound to the disaccharide melibiose, Galα(1,6) Glc, and the trisaccharide globotriose, Galα(1,4) Galβ(1,4) Glc. Toxicity of the lectin was found to be low with an LC50 value of 384.53 μg/mL, determined using the Artemia nauplii lethality assay. A fluorescence assay was carried out to evaluate the glycan-dependent binding of MytiLec-1 to Artemia nauplii. The lectin strongly agglutinated Ehrlich ascites carcinoma (EAC) cells cultured in vivo in Swiss albino mice. When injected intraperitoneally to the mice at doses of 1.0 mg/kg/day and 2.0 mg/kg/day for five consecutive days, MytiLec-1 inhibited 27.62% and 48.57% of cancer cell growth, respectively. Antiproliferative activity of the lectin against U937 and HeLa cells was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in vitro in RPMI-1640 medium. MytiLec-1 internalized into U937 cells and 50 μg/mL of the lectin inhibited their growth of to 62.70% whereas 53.59% cell growth inhibition was observed against EAC cells when incubated for 24 h. Cell morphological study and expression of apoptosis-related genes (p53, Bax, Bcl-X, and NF-κB) showed that the lectin possibly triggered apoptosis in these cells.

DOI： 10.3390/md17090502

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担当区分：最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.marpolbul.2019.02.061

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier {BV}  

DOI： 10.1016/j.ijbiomac.2018.11.222

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MDPI AG  

We identified a lectin (carbohydrate-binding protein) belonging to the complement 1q(C1q) family in the feather star Anneissia japonica (a crinoid pertaining to the phylum Echinodermata). The combination of Edman degradation and bioinformatics sequence analysis characterized the primary structure of this novel lectin, named OXYL, as a secreted 158 amino acid-long globular head (sgh)C1q domain containing (C1qDC) protein. Comparative genomics analyses revealed that OXYL pertains to a family of intronless genes found with several paralogous copies in different crinoid species. Immunohistochemistry assays identified the tissues surrounding coelomic cavities and the arms as the main sites of production of OXYL. Glycan array confirmed that this lectin could quantitatively bind to type-2 N-acetyllactosamine (LacNAc: Galβ1-4GlcNAc), but not to type-1 LacNAc (Galβ1-3GlcNAc). Although OXYL displayed agglutinating activity towards Pseudomonas aeruginosa, it had no effect on bacterial growth. On the other hand, it showed a significant anti-biofilm activity. We provide evidence that OXYL can adhere to the surface of human cancer cell lines BT-474, MCF-7, and T47D, with no cytotoxic effect. In BT-474 cells, OXYL led to a moderate activation of the p38 kinase in the MAPK signaling pathway, without affecting the activity of caspase-3. Bacterial agglutination, anti-biofilm activity, cell adhesion, and p38 activation were all suppressed by co-presence of LacNAc. This is the first report on a type-2 LacNAc-specific lectin characterized by a C1q structural fold.

DOI： 10.3390/md17020136

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Acta Pharmaceutica Sciencia  

DOI： 10.23893/1307-2080.aps.05704

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.4052/tigg.1717.1j

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担当区分：責任著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Fujii et al TIGG 30 177J155-188_1717.1J.pdf

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1186/s12864-017-4012-z

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/s41598-017-06332-7

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/jth.13617

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Fujii et al (2017) Glycoconj J 34 85-94.pdf

DOI： 10.1007/s10719-016-9731-x

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Sugawara et al (2017) Glycoconj J 34 127-138.pdf

DOI： 10.1007/s10719-016-9739-2

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/elps.201600346

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記述言語：英語  

添付ファイル： Ch33 Hasan et al Marine Glycobiology Principles and Applications KIM 2016 CRC.pdf

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記述言語：英語  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/srep28344

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/md14050092

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kabir et al (2016) Int J Biol Macromol 84 62-68.pdf

DOI： 10.1016/j.ijbiomac.2015.12.006

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kamitori et al (2016) Regenerative Therapy 3 11-14.pdf

DOI： 10.1016/j.reth.2016.01.006

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kawsar et al (2016) Int Lett Chem Phys Astro 64 95-105.pdf

DOI： 10.18052/www.scipress.com/ilcpa.64.95

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Growing Science  

添付ファイル： Chowdhury et al (2016) Curr Chem Lett 5 83-92.pdf

DOI： 10.5267/j.ccl.2015.12.001

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掲載種別：研究論文（学術雑誌）   出版者・発行元：MDPI AG  

DOI： 10.3390/molecules21010129

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Scientific Research Publishing, Inc.  

添付ファイル： Kawsar et al (2015) Int J Org Chem USA 5(4) 232-245.pdf

DOI： 10.4236/ijoc.2015.54023

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Hasan et al (2015) Mar Drugs 13(12)7377-7389.pdf

DOI： 10.3390/md13127071

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-BLACKWELL  

Toward the development of ecotoxicology methods to investigate microbial markers of impacts of hydrocarbon processing activities, DNA adductomic analyses were conducted on a sphingomonad soil bacterium. From growing cells that were exposed or unexposed to acrolein, a commonly used biocide in hydraulic fracturing processes, DNA was extracted, digested to 2'-deoxynucleosides and analyzed by liquid chromatography-positive ionization electrospray-tandem mass spectrometry in selected reaction monitoring mode transmitting the [M + H](+) &gt; [M + H - 116](+) transition over 100 transitions. Overall data shown as DNA adductome maps revealed numerous putative DNA adducts under both conditions with some occurring specifically for each condition. Adductomic analyses of triplicate samples indicated that elevated levels of some targeted putative adducts occurred in exposed cells. Two exposure-specific adducts were identified in exposed cells as 3-(2'-deoxyribosyl)-5,6,7,8-tetrahydro-6-hydroxy- (and 8-hydroxy-)pyrimido[1,2-a]-purine-(3H)-one (6- and 8-hydroxy-PdG) following synthesis of authentic standards of these compounds and subsequent analyses. A time course experiment showed that 6- and 8-hydroxy-PdG were detected in bacterial DNA within 30 min of acrolein exposure but were not detected in unexposed cells. This work demonstrated the first application of DNA adductomics to examine DNA damage in a bacterium and sets a foundation for future work.

DOI： 10.1002/mbo3.283

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kawsar et al (2015) Int Lett Chem Phys Astro 58 122-136.pdf

DOI： 10.18052/www.scipress.com/ilcpa.58.122

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1099/ijs.0.000356

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10532-015-9739-0

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/mbo3.283

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J-GLOBAL

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kawsar et al (2015) Current Res Chem 7(2), 21-33.pdf

DOI： 10.3923/crc.2015.21.33

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Koide et al (2015) Ann. Marine Biol. Res. 2, 1005.pdf

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCI LTD  

Comprehensive analyses of the biotransformation of the N-heteroaromatic environmental pollutant, indole, by a newly isolated soil bacterium designated strain KK10, were conducted by liquid chromatography negative electrospray ionization tandem mass spectrometry (LC/ESI(-)-MS/MS). Numerous indole bioproducts were revealed and provided evidence for multiple pathways of indole biotransformation by this strain including unreported pathways. Oxidation of the N-heterocyclic ring of indole and ring cleavage through N-formylanthranilic acid and gentisic acid was characterized in addition to a carbocyclic ring-cleavage pathway which was documented for the first time. Three carbocyclic aromatic ring cleavage products of indole were proposed and the structure of 2,3-pyrrole-dicarboxylic acid was confirmed following synthesis and analyses of an authentic standard of this compound. Eight downstream ring-fission products were identified overall and these results confirmed the lower pathways of indole biotransformation. Indigo, indirubin and other indigoid compounds were produced by strain KK10 and confirmed N-heterocyclic-ring oxidations in the upper pathways. Nearly complete sequencing of the 16S rRNA gene of strain KK10 and phylogenetic analysis revealed that it was a new member of the genus Cupriavidus. Cupriavidus strains that biotransform N-heteroaromatic pollutants have not been described. These results expand both our understanding of the versatile catabolic capabilities of members of the genus Cupriavidus and provide evidence for alternative biotransformation mechanisms for indole in the environment by bacteria. (C) 2014 Elsevier Ltd. All rights reserved.

添付ファイル： Fukuoka et al (2015) Int Biodetrio Biodegra 97 13-24.pdf

DOI： 10.1016/j.ibiod.2014.11.007

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCI LTD  

Comprehensive analyses of the biotransformation of the N-heteroaromatic environmental pollutant, indole, by a newly isolated soil bacterium designated strain KK10, were conducted by liquid chromatography negative electrospray ionization tandem mass spectrometry (LC/ESI(-)-MS/MS). Numerous indole bioproducts were revealed and provided evidence for multiple pathways of indole biotransformation by this strain including unreported pathways. Oxidation of the N-heterocyclic ring of indole and ring cleavage through N-formylanthranilic acid and gentisic acid was characterized in addition to a carbocyclic ring-cleavage pathway which was documented for the first time. Three carbocyclic aromatic ring cleavage products of indole were proposed and the structure of 2,3-pyrrole-dicarboxylic acid was confirmed following synthesis and analyses of an authentic standard of this compound. Eight downstream ring-fission products were identified overall and these results confirmed the lower pathways of indole biotransformation. Indigo, indirubin and other indigoid compounds were produced by strain KK10 and confirmed N-heterocyclic-ring oxidations in the upper pathways. Nearly complete sequencing of the 16S rRNA gene of strain KK10 and phylogenetic analysis revealed that it was a new member of the genus Cupriavidus. Cupriavidus strains that biotransform N-heteroaromatic pollutants have not been described. These results expand both our understanding of the versatile catabolic capabilities of members of the genus Cupriavidus and provide evidence for alternative biotransformation mechanisms for indole in the environment by bacteria. (C) 2014 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.ibiod.2014.11.007

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Academic Journals Inc.  

添付ファイル： Kawsar 2015 Int J Biol Chem 9(6) 269-282.pdf

DOI： 10.3923/ijbc.2015.269.282

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掲載種別：研究論文（学術雑誌）   出版者・発行元：The Society of Japanese Women Scientists  

DOI： 10.5939/sjws.15006

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記述言語：英語  

DOI： 10.1007/s10695-014-9957-0

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記述言語：英語  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER  

Rhamnose-binding lectin (RBL) is one of the animal lectin categories which take part in the innate immune responses of fish. Osmerus lanceolatus lectin (OLL) from shishamo smelt eggs is an RBL composed of two tandem-repeated domains, both of which are considered to be a carbohydrate-recognition domain. SAL, catfish (Silurus asotus) egg RBL composed of three domains, binds to Burkitt's lymphoma Raji cells through globotriaosylceramide (Gb3) carbohydrate chain and to reduce cell size and growth by altering membrane composition without causing cell death. In this experiment, we tried to compare the binding effects of these two RBLs on Raji cells. Flow cytometric and fluorescence microscopic analyses revealed that OLL also directly bound to and shrunk Raji cells with ten times less reactivity than SAL but reduced cell growth with decreasing cell viability. Anti-Gb3 antibody completely blocked the binding of SAL to Raji cells but not that of OLL. In addition, the direct bindings of OLL and SAL to Raji cells were comparably inhibited by melibiose, but lactose was more effective inhibitor for the binding of OLL than that of SAL. These results suggest that OLL has slightly different cell-binding property compared with SAL and binds not only to Gb3 but also to the other carbohydrate receptor-bearing beta-galactoside chains. The quantitative RT-PCR analysis revealed that SAL induced the expression of TNF-alpha but not of IFN-gamma, IL-1 beta, and IL-10. Thus, SAL-induced cytostatic effect on Raji cells might be partially caused by TNF-alpha-mediated signaling pathway.

DOI： 10.1007/s10695-014-9948-1

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Rhamnose-binding lectin (RBL) is one of the animal lectin categories which take part in the innate immune responses of fish. Osmerus lanceolatus lectin (OLL) from shishamo smelt eggs is an RBL composed of two tandem-repeated domains, both of which are considered to be a carbohydrate-recognition domain. SAL, catfish (Silurus asotus) egg RBL composed of three domains, binds to Burkitt's lymphoma Raji cells through globotriaosylceramide (Gb3) carbohydrate chain and to reduce cell size and growth by altering membrane composition without causing cell death. In this experiment, we tried to compare the binding effects of these two RBLs on Raji cells. Flow cytometric and fluorescence microscopic analyses revealed that OLL also directly bound to and shrunk Raji cells with ten times less reactivity than SAL but reduced cell growth with decreasing cell viability. Anti-Gb3 antibody completely blocked the binding of SAL to Raji cells but not that of OLL. In addition, the direct bindings of OLL and SAL to Raji cells were comparably inhibited by melibiose, but lactose was more effective inhibitor for the binding of OLL than that of SAL. These results suggest that OLL has slightly different cell-binding property compared with SAL and binds not only to Gb3 but also to the other carbohydrate receptor-bearing β-galactoside chains. The quantitative RT-PCR analysis revealed that SAL induced the expression of TNF-α but not of IFN-γ, IL-1β, and IL-10. Thus, SAL-induced cytostatic effect on Raji cells might be partially caused by TNF-α-mediated signaling pathway.

添付ファイル： Hosono et al (2014) Fish Physiol Biochem 40 1559-1572.pdf

DOI： 10.1007/s10695-014-9948-1

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Hasan et al (2014) Molecules 19_13990-14003.pdf

DOI： 10.3390/molecules190913990

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Hasan et al (2014) Indian J Biochem Biophys 51(2) 142-148.pdf

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

文科省国費留学生Imtiaj Hasan氏(2013年3月YCU博士課程進学)の本邦初第一著者論文を出版できました。ポテトレクチンによるエールリッヒがん細胞死活性を報告

添付ファイル： Hasan et al (2014) African J Biotechnol 13(15) 1679-1685.pdf

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY  

A pathway for the biotransformation of the environmental pollutant and high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by a soil bacterium was constructed through analyses of results from liquid chromatography negative electrospray ionization tandem mass spectrometry (LC/ESI(-)-MS/MS). Exposure of Sphingobium sp. strain KK22 to benzo[k]fluoranthene resulted in transformation to four-, three- and two-aromatic ring products. The structurally similar four- and three-ring non-alternant PAHs fluoranthene and acenaphthylene were also biotransformed by strain KK22, and LC/ESI(-)-MS/MS analyses of these products confirmed the lower biotransformation pathway proposed for benzo[k]fluoranthene. In all, seven products from benzo[k]fluoranthene and seven products from fluoranthene were revealed and included previously unreported products from both PAHs. Benzo[k]fluoranthene biotransformation proceeded through ortho-cleavage of 8,9-dihydroxy-benzo[k]fluoranthene to 8-carboxyfluoranthenyl-9-propenic acid and 9-hydroxy-fluoranthene-8-carboxylic acid, and was followed by meta-cleavage to produce 3-(2-formylacenaphthylen-1-yl)-2-hydroxy-prop-2-enoic acid. The fluoranthene pathway converged with the benzo[k]fluoranthene pathway through detection of the three-ring product, 2-formylacenaphthylene-1-carboxylic acid. Production of key downstream metabolites, 1,8-naphthalic anhydride and 1-naphthoic acid from benzo[k]fluoranthene, fluoranthene and acenaphthylene biotransformations provided evidence for a common pathway by strain KK22 for all three PAHs through acenaphthoquinone. Quantitative analysis of benzo[k]fluoranthene biotransformation by strain KK22 confirmed biodegradation. This is the first pathway proposed for the biotransformation of benzo[k]fluoranthene by a bacterium.

DOI： 10.1111/1751-7915.12102

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley  

ロバート・カナリー研究指導博士院生、前田亜鈴悠筆頭論文。環境保全微生物スフィンゴビウム属KK22株の多環芳香族毒物の分解代謝過程をLC/MSMSで同定。横浜市大連携大学院・海洋研究開発機構との共同研究

添付ファイル： 2013 Maeda Kanaly et al Microbial Biotechology.pdf

DOI： 10.1111/1751-7915.12102

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CiNii Research

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Ogawa et al (2014) Glycoconj J 31(2) 171-184.pdf

DOI： 10.1007/s10719-013-9513-7

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Osterreichische Apotheker-Verlagsgesellschaft m.b.H.  

添付ファイル： Kawsar et al (2014) Sci Pharm 82 1-20.pdf

DOI： 10.3797/scipharm.1308-03

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Osmerus (Spirinchus) lanceolatus egg lectin (OLL) is a member of the rhamnose-binding lectin (RBL) family which is mainly found in aqueous beings. cDNA of OLL was cloned, and its genomic architecture was revealed. The deduced amino acid (aa) sequence indicated that OLL was composed of 213 aa including 95 aa of domain N and 97 aa of domain C. N and C showed 73 % sequence identity and contained both -ANYGR- and -DPC-KYL-peptide motifs which are conserved in most of the RBL carbohydrate recognition domains. The calculated molecular mass of mature OLL was 20,852, consistent with the result, and 20,677.716, from mass spectrometry. OLL was encoded by eight exons: exons 1 and 2 for a signal peptide; exons 3-5 and 6-8 for N- and C-domains, respectively. Surface plasmon resonance spectrometric analyses revealed that OLL showed comparable affinity for Galα- and β-linkages, whereas Silurus asotus lectin (SAL), a catfish RBL, bound preferentially to α-linkages of neoglycoproteins. The Kd values of OLL and SAL against globotriaosylceramide (Gb3) were 1.69 × 10⁻⁵ M for and 2.81 × 10⁻⁶ M, respectively. Thus, the carbohydrate recognition property of OLL is slightly different from that of SAL. On the other hand, frontal affinity chromatography revealed that both OLL and SAL interacted with only glycolipid-type oligosaccharides such as Gb3 trisaccharides, not with N-linked oligosaccharides. The domain composition of these RBLs and an analytical environment such as the "cluster effect" of a ligand might influence the binding between RBL and sugar chains.

添付ファイル： Hosono et al (2013) Fish Physiol Biochem 39 1619-1630.pdf

DOI： 10.1007/s10695-013-9814-6

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

JSPS海外特別研究員SMアベ・カウサル博士(現チッタゴン大学理学部化学科教授、受け入れ期間2009-11年)の帰国後初論文。有機合成した人工糖による顕著な抗菌効果を報告

添付ファイル： Kawsar et al (2013) Hacettepe J Biol Chem 41(3) 195-206.pdf

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kunihiro et al (2013) Appl Environ Microbiol 79 4410.pdf

DOI： 10.1128/AEM.01129-13

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記述言語：英語   掲載種別：論文集(書籍)内論文   出版者・発行元：John Wiley and Sons  

添付ファイル： Ozeki et al (2013) Chapt 8 Marine Proteins & Peptides Wiley & Blackwell.pdf

DOI： 10.1002/9781118375082.ch8

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Yasumitsu et al (2013) Protein Pep Lett 20 213-217.pdf

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：American Society for Microbiology  

添付ファイル： Maeda et al Kanaly e00911-13.full.pdf

DOI： 10.1128/genomeA.00911-13

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Fujii et al (2012) J Biol Chem 287 44772-44783.pdf

DOI： 10.1074/jbc.M112.418012

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Yamamoto et al Biochemistry(2012) 51 5329-5338.pdf

DOI： 10.1021/bi300442c

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Matsumoto et al (2012) Toxins 4 323-338.pdf

DOI： 10.3390/toxins4050323

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Fujii et al (2012) Protein J 31,(1) 15-26.pdf

DOI： 10.1007/s10930-011-9369-2

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Yasumitsu et al (2011) In Vitro Cell Dev Biol 47, 728-734.pdf

DOI： 10.1007/s11626-011-9462-z

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kawsar et al (2011) Protein Journal 30 509-519.pdf

DOI： 10.1007/s10930-011-9356-7

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Matsumoto et al (2011) CBP 158 266-273.pdf

DOI： 10.1016/j.cbpb.2010.12.004

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Fujii et al CBP 158 30-37 (2011).pdf

DOI： 10.1016/j.cbpb.2010.09.002

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kawsar et al (2011) Phytologia Balcanica 17, 347-354.pdf

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掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Yasumitsu et al (2010) Anal Biochem 406 86-88.pdf

DOI： 10.1016/j.ab.2010.06.035

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kawsar et al (2010)Int J Biol Life Sci 6 31-37.pdf

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Yasumitsu et al (2010) Electrophoresis 31 1913-1917.pdf

DOI： 10.1002/elps.200900524

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.2174/092986610791112648

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Zenodo  

添付ファイル： Kawsar et al (2010)Int J Biol Life Sci 6 44-50.pdf

DOI： 10.5281/ZENODO.1057039

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掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kawsar et al (2010) Arch Biol Sci Belgrade 62 1927-1038.pdf

DOI： 10.2298/ABS1004027K

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kawsar et al (2009) Biochemistry(Moscow)74, 709-716.pdf

DOI： 10.1134/S0006297909070025

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：6  

産業総合研究所糖鎖医工学研究センターのレクチンデータベース構築のために提供したウシガエル卵ガレクチンの糖鎖結合プロファイルは、他のβガラクトシド結合性ガレクチンと異なり、キノコやカイメン由来のガレクチン同様に血液型A型やフォルスマン抗原などN-アセチルガラクトサミンンを特異的に認識し、細胞接着も行うことが見いだせられました

添付ファイル： Kawsar et al (2009) PPL 16,677-684.pdf

DOI： 10.2174/092986609788490104

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kawsar et al (2009) CBP 152, 382-389.pdf

DOI： 10.1016/j.cbpb.2009.01.009

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DOI： 10.2174/092986609788490104

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3923/ijbc.2009.42.48

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Fujii et al (2009) J Biol Sci 9 (4) 319-325.pdf

DOI： 10.3923/jbs.2009.319.325

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE INC  

添付ファイル： Kawsar et al (2008) CBP 150 pp349-357.pdf

DOI： 10.1016/j.cbpb.2008.04.004

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE INC  

DOI： 10.1016/j.cbpb.2008.04.004

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3923/jbs.2008.1051.1056

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3923/ajpp.2008.165.172

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE INC  

Galactoside-binding lectin was purified from the snake venom of Crotalus ruber by affinity chromatography on a lactose-agarose column, and the complete amino acid sequence was determined. The C. ruber venom lectin (CRL) showed a single band of 28 kDa by SDS-polyacrylamide electrophoresis under non-reducing conditions, but it showed a single band of 15 kDa under reducing conditions, indicating that CRL is a disulfide-linked homodimer of 15 kDa subunit. CRL specifically recognized beta-galactosides such as thiodigalactoside followed by Nacetylgalactosamine when examined with their inhibitory effects on CPL-induced hemagglutination. A CRL subunit was composed of 135 residues containing nine Cys residues and showed a high similarity to other C-type galactoside-binding lectins from snake venoms. C. atrox lectin (CAL) showed almost the same sequence except for eight amino acid residues. Neither CRL nor CAL induced platelet aggregation by itself or inhibited platelet aggregation mediated by von Willebrand factor or fibrinogen with agonists. CRL showed a similar oligomeric form and the sugar specificity as CAL, but it showed different divalent cation sensitivity such as Mn2+ and Ni2+. Homology modeling suggested that the amino acid substitution found in CRL does not affect sugar recognition of the lectin but might alter the conformation and influence the sugar binding pocket induced by the metal-ion binding. (c) 2006 Elsevier Inc. All rights reserved.

添付ファイル： Hamako et al (2007) Comp Biochem Physiol partB146 299-306.pdf

DOI： 10.1016/j.cbpb.2006.11.022

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE INC  

Galactoside-binding lectin was purified from the snake venom of Crotalus ruber by affinity chromatography on a lactose-agarose column, and the complete amino acid sequence was determined. The C. ruber venom lectin (CRL) showed a single band of 28 kDa by SDS-polyacrylamide electrophoresis under non-reducing conditions, but it showed a single band of 15 kDa under reducing conditions, indicating that CRL is a disulfide-linked homodimer of 15 kDa subunit. CRL specifically recognized beta-galactosides such as thiodigalactoside followed by Nacetylgalactosamine when examined with their inhibitory effects on CPL-induced hemagglutination. A CRL subunit was composed of 135 residues containing nine Cys residues and showed a high similarity to other C-type galactoside-binding lectins from snake venoms. C. atrox lectin (CAL) showed almost the same sequence except for eight amino acid residues. Neither CRL nor CAL induced platelet aggregation by itself or inhibited platelet aggregation mediated by von Willebrand factor or fibrinogen with agonists. CRL showed a similar oligomeric form and the sugar specificity as CAL, but it showed different divalent cation sensitivity such as Mn2+ and Ni2+. Homology modeling suggested that the amino acid substitution found in CRL does not affect sugar recognition of the lectin but might alter the conformation and influence the sugar binding pocket induced by the metal-ion binding. (c) 2006 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.cbpb.2006.11.022

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/S0197-0186(02)00092-X

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Matsui et al (2001) Biochem Biophys Acta 1525 50-57.pdf

DOI： 10.1016/S0304-4165(00)00170-7

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley-Blackwell  

添付ファイル： Ozeki (1998) IUBMB Life 45 989-995.pdf

DOI： 10.1002/iub.7510450516

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Uchiyama et al (1997) Glycobiology 7 1159-1165.pdf

DOI： 10.1093/glycob/7.8.1159

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記述言語：英語  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Ozeki et al (1997) CBP 118B 1-6.pdf

DOI： 10.1016/S0305-0491(97)00014-X

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Ozeki Y (1997) IUBMB Life 41(3) 633-640.pdf

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Matsui et al (1997) J Biochem121 376-381.pdf

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：JAPANESE BIOCHEMICAL SOC  

The binding of human von Willebrand factor (vWF) to a variety of extracellular matrix components immobilized on plates and the binding of vWF to platelet glycoprotein It, (GPIb) after interacting with these matrix components mere examined by means of an enzyme-linked immunosorbent assay. vWF preferably bound to type III collagen, whereas it did not significantly bind to type I, IV, V, or VI collagen, fibronectin, laminin, elastin, or proteoglycans. Soluble type III collagen did not bind to vWF coated on plates and showed a little effect on the vWF binding to the immobilized collagen, suggesting that solid-phase collagen is important for the interaction with vWF. When glycocalicin, the N-terminal carbohydrate-rich extracellular domain of GPIb alpha exhibiting the vWF-binding activity, was added to vWF bound to collagen type III, no significant binding of glycocalicin was observed, but it bound to vWF in the presence of botrocetin, a vWF modulator protein isolated from Bothrops jararaca snake venom. These results indicate that vWP immobilized on collagen can interact with GPIb but that the binding of vWF to the collagen matrix alone is insufficient for modulating vWF so that it interacts with GPIb under static conditions. Another unknown physiological modulator functionally mimicking botrocetin or high-shear stress may be involved in the platelet adhesion to extracellular mat-fix in the early stage of hemostasis.

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kou et al J Clin Invest 98(12)2813-2813.pdf

DOI： 10.1172/JCI119109

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1006/bbrc.1996.1345

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掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/0305-0491(95)00121-2

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Hamako et al (1993) Comp Biochem Physiol 106B 949-954.pdf

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Ozeki et al (1995) Glycobiology 5, 255-261.pdf

DOI： 10.1093/glycob/5.2.255

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Ozeki et al (1995) Exp Cell Res 216, 318-324.pdf

DOI： 10.1006/excr.1995.1040

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Ozeki et al (1994) Arch Biochem Biophys 308(1) 306-310.pdf

DOI： 10.1006/abbi.1994.1043

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Oxford University Press  

添付ファイル： Matsui et al (1994) J Biochem116 1127-1133.pdf

DOI： 10.1093/oxfordjournals.jbchem.a124638

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

1. Asparagine-linked sugar chains released by hydrazinolysis from IgGs of porcine, equine, bovine, goat, ovine, canine, rabbit, guinea-pig and rat were comparatively analyzed by microsequencing and lectin affinity chromatography.
2. Sugar chains of all IgGs basically consisted of biantennary complex-type oligosaccharides containing 0-2 sialic acid residue(s). More than 70% of the oligosaccharides were neutral, except for guinea-pig IgG, and fucosylated trimannosyl core structures were dominant except for rabbit IgG. Bisecting N-acetylglucosamine residue was absent in porcine and equine IgGs.
3. A large quantity of galactose-less oligosaccharides were present in IgGs of porcine, equine, canine and rat.

DOI： 10.1016/0305-0491(93)90056-B

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier {BV}  

添付ファイル： Hamako et al (1993) Comp Biochem Physiol 106B 949-954.pdf

DOI： 10.1016/0305-0491(93)90056-b

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Usami et al (1993) PNAS 90 928-932.pdf

DOI： 10.1073/pnas.90.3.928

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/0014-5793(91)81055-D

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Ozeki et al (1991) BBRC 178 404-413.pdf

DOI： 10.1016/0006-291X(91)91828-Z

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Ozeki et al (1991) Biochemistry 30 2391-2394.pdf

DOI： 10.1021/bi00223a014

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： Hattori et al (1990) J. J. Breed 40, 295-301.pdf

DOI： 10.1270/jsbbs1951.40.295

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総ページ数：540   担当ページ：407-418   記述言語：英語   著書種別：学術書

ASIN

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総ページ数：744   担当ページ：407-418   記述言語：英語   著書種別：学術書

ASIN

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総ページ数：816   担当ページ：167-184   記述言語：英語   著書種別：学術書

ASIN

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記述言語：英語   会議種別：口頭発表（一般）  

開催地：中国、青島市  

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添付ファイル： 2018_June_09 CGM.jpg

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記述言語：英語   会議種別：口頭発表（基調）  

開催地：Mohali, Punjab, India  

添付ファイル： Final Program of CARBO-XXIX.pdf

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：インド共和国 マハーラーシュトラ州 ムンバイ大学  

添付ファイル： Final Program 102nd ISC.pdf

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記述言語：日本語   会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

添付ファイル： 2012年3月6日ライフイノベーションのための糖鎖関連シーズ神奈川県受託事業講演.pdf

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記述言語：日本語   会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

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記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

開催地：Honolulu, Hawaii  

添付ファイル： Hasan et al (2014) Glycobiology 24 1053-1231 Conference abstract.pdf

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記述言語：日本語   会議種別：口頭発表（招待・特別）  

開催地：長崎県佐世保市  

添付ファイル： 2014年9月11日長崎国際大学要旨大関泰裕.pdf

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記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：インド共和国、ベンガルール  

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添付ファイル： 2019-06-08_.jpg

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記述言語：英語   会議種別：口頭発表（一般）  

開催地：Baltimore, Maryland, USA  

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会議種別：口頭発表（招待・特別）  

開催地：秋葉原コンベンションホール５階カンファレンスフロア/５B会議室  

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会議種別：シンポジウム・ワークショップ パネル（指名）  

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発表場所：Essentials of Glycobiology  

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その他リンク： https://www.ncbi.nlm.nih.gov/books/NBK579951/

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発表場所：横浜市庁舎アトリウム  

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Ch 31 R-type Lectins
Richard D Cummings, Ronald L Schnaar, & Yasuhiro Ozeki

DOI： 10.1101/9781621824213

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発表場所：横浜市立大学  

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添付ファイル： 第23回比較グライコーム研究会2021ポスターfinal.pdf

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その他リンク： https://www.yokohama-cu.ac.jp/english/news/2022/20220323_23rd-glycomeh.html

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Collaboration of lectin researches with crystallographers

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その他リンク： https://www2.chubu.ac.jp/digibook/wpwt/216/html5.html#page=15

発表場所：横浜市立大学  

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添付ファイル： onlineシンポ2020年8月21日比較グライコーム研究会(6_05版).pdf

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発表場所：北海道 札幌市 札幌コンベンションセンター  

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発表場所：札幌コンベンションセンター、札幌市  

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その他リンク： http://www-user.yokohama-cu.ac.jp/~english/index.php/2020/06/11/a-ycu-alumnus-prof-dr-s-m-abe-kawsar-university-of-chittagong-bangladesh-published-two-academic-books-from-a-german-publisher/

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発表場所：インド農業科学大学 インド共和国、ベンガルール  

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発表場所：ベンガル―ル、インド共和国  

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発表場所：MDPI 出版 (スイス・バーゼル)  

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発表場所：横浜市立大 木原生物学研究所  

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発表場所：大さん橋ホール 横浜  

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発表場所：7日(木)、8日(金) 於お茶の水女子大  

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発表場所：秋葉原コンベンションホール５階カンファレンスフロア/５B会議室  

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発表場所：さくらWORKS〈関内〉イベントスペース  

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作品分類：教材  

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発表場所：横浜市立大学  

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作品分類：Web Service  

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発表場所：文部科学記者会・横浜市政記者クラブ  

2016年6月16日 日刊みなと新聞 掲載

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発表場所：横浜市立大学ウェッブサイト  

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発表場所：Croatia, Sprit  

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添付ファイル： 2014 - Ozeki - YCU, San Diego retreat program - SA_rev2.pdf

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発表場所：サンフォード・バーナム医学研究所、UCSD、スクリップス海洋研究所 他  

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発表場所：Orland, FL, USA  

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発表場所：横浜市立大学  

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発表場所：第22回国際複合糖質学会(Glyco22) 中国、大連  

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発表場所：海洋研究開発機構  

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発表場所：Protein Data Bank  

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作品分類：教材   発表場所：Yokohama City University  

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作品分類：教材