Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

SeviL, a galactoside-binding lectin previously isolated from the mussel Mytilisepta virgata, was demonstrated to trigger apoptosis in HeLa ovarian cancer cells. Here, we show that this lectin can promote the polarization of macrophage cell lines toward an M1 functional phenotype at low concentrations. The administration of SeviL to monocyte and basophil cell lines reduced their growth in a dose-dependent manner. However, low lectin concentrations induced proliferation in the RAW264.7 macrophage cell line, which was supported by the significant up-regulation of TOM22, a component of the mitochondrial outer membrane. Furthermore, the morphology of lectin-treated macrophage cells markedly changed, shifting from a spherical to an elongated shape. The ability of SeviL to induce the polarization of RAW264.7 cells to M1 macrophages at low concentrations is supported by the secretion of proinflammatory cytokines and chemokines, as well as by the enhancement in the expression of IL-6- and TNF-α-encoding mRNAs, both of which encode inflammatory molecular markers. Moreover, we also observed a number of accessory molecular alterations, such as the activation of MAP kinases and the JAK/STAT pathway and the phosphorylation of platelet-derived growth factor receptor-α, which altogether support the functional reprogramming of RAW264.7 following SeviL treatment. These results indicate that this mussel β-trefoil lectin has a concentration-dependent multifunctional role in regulating cell proliferation, phenotype, and death in macrophages, suggesting its possible involvement in regulating hemocyte activity in vivo.

DOI： 10.3390/md22060269

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.52794/hujpharm.1354419

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Language：English   Publishing type：Research paper (scientific journal)  

A Cucurbita phloem exudate lectin (CPL) from summer squash (Cucurbita pepo) fruits was isolated and its sugar-binding properties and biological activities were studied. The lectin was purified by affinity chromatography and the hemagglutination assay method was used to determine its pH, heat stability, metal-dependency and sugar specificity. Antimicrobial and anticancer activities were also studied by disc diffusion assays and in vivo and in vitro methods. The molecular weight of CPL was 30 ± 1 KDa and it was stable at different pH (5.0 to 9.0) and temperatures (30 to 60 °C). CPL recovered its hemagglutination activity in the presence of Ca2+. 4-nitrophenyl-α-D-glucopyranoside, lactose, rhamnose and N-acetyl-D-glucosamine strongly inhibited the activity. With an LC50 value of 265 µg/mL, CPL was moderately toxic and exhibited bacteriostatic, bactericidal and antibiofilm activities against different pathogenic bacteria. It also exhibited marked antifungal activity against Aspergillus niger and agglutinated A. flavus spores. In vivo antiproliferative activity against Ehrlich ascites carcinoma (EAC) cells in Swiss albino mice was observed when CPL exerted 36.44% and 66.66% growth inhibition at doses of 3.0 mg/kg/day and 6.0 mg/kg/day, respectively. A 12-day treatment by CPL could reverse their RBC and WBC counts as well as restore the hemoglobin percentage to normal levels. The MTT assay of CPL performed against human breast (MCF-7) and lung (A-549) cancer cell lines showed 29.53% and 18.30% of inhibitory activity at concentrations of 128 and 256 µg/mL, respectively.

DOI： 10.3390/molecules29112531

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Language：English   Publishing type：Research paper (scientific journal)  

This research describes the synthesis by an environmentally-friendly method, microwave irradiation, development and analysis of three novel and one previously identified Schiff base derivative as a potential inhibitor of bovine xanthine oxidase (BXO), a key enzyme implicated in the progression of gout. Meticulous experimentation revealed that these compounds (10, 9, 4, and 7) have noteworthy inhibitory effects on BXO, with IC50 values ranging from 149.56 µM to 263.60 µM, indicating their good efficacy compared to that of the standard control. The validation of these results was further enhanced through comprehensive in silico studies, which revealed the pivotal interactions between the inhibitors and the catalytic sites of BXO, with a particular emphasis on the imine group (-C = N-) functionalities. Intriguingly, the compounds exhibiting the highest inhibition rates also showcase advantageous ADMET profiles, alongside encouraging initial assessments via PASS, hinting at their broad-spectrum potential. The implications of these findings are profound, suggesting that these Schiff base derivatives not only offer a new vantage point for the inhibition of BXO but also hold considerable promise as innovative therapeutic agents in the management and treatment of gout, marking a significant leap forward in the quest for more effective gout interventions.

DOI： 10.1016/j.jsps.2024.102062

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Nucleoside analogs have been widely used as antiviral, antitumor, and antiparasitic agents due to their ability to inhibit nucleic acid synthesis. Adenosine, cytidine, guanosine, thymidine and uridine analogs such as didanosine, vidarabine, remdesivir, gemcitabine, lamivudine, acyclovir, abacavir, zidovusine, stavudine, and idoxuridine showed remarkable anticancer and antiviral activities. In our previously published articles, our main intention was to develop newer generation nucleoside analogs with acylation-induced modification of the hydroxyl group and showcase their biological potencies. In the process of developing nucleoside analogs, in silico studies play an important role and provide a scientific background for biological data. Molecular interactions between drugs and receptors followed by assessment of their stability in physiological environments, help to optimize the drug development process and minimize the burden of unwanted synthesis. Computational approaches, such as DFT, FMO, MEP, ADMET prediction, PASS prediction, POM analysis, molecular docking, and molecular dynamics simulation, are the most popular tools to culminate all preclinical study data and deliver a molecule with maximum bioactivity and minimum toxicity. Although clinical drug trials are crucial for providing dosage recommendations, they can only indirectly provide mechanistic information through researchers for pathological, physiological, and pharmacological determinants. As a result, in silico approaches are increasingly used in drug discovery and development to provide mechanistic information of clinical value. This article portrays the current status of these methods and highlights some remarkable contributions to the development of nucleoside analogs with optimized bioactivity.

DOI： 10.2174/0113895575258033231024073521

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.ijbiomac.2023.127628

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.jsps.2023.101804

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Authorship：Last author   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/md21120614

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Authorship：Last author   Language：English  

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

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Other Link： https://www.trendscarbo.com/aboutjournal.php

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/molecules28010219

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/md20100613

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

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Authorship：Last author   Language：English   Publisher：Cold Spring Harbor Laboratory Press  

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Other Link： https://www.ncbi.nlm.nih.gov/books/NBK579918/toc/?report=reader

Language：English   Publishing type：Research paper (scientific journal)  

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Language：English   Publishing type：Research paper (scientific journal)  

Carbohydrate esters are significant in medicinal chemistry because of their efficacy for the synthesis of biologically active drugs. In the present study, methyl β-D-galactopyranoside (MGP) was treated with various acyl halides to produce 6-O-acyl MGP esters by direct acylation method with an excellent yield. To obtain newer products for antimicrobial assessment studies, the 6-O-MGP esters were further modified into 2,3,4-tri-O-acyl MGP esters containing a wide variety of functionalities in a single molecular framework. The chemical structures of the newly synthesized compounds were elucidated by analyzing their physicochemical, elemental, and spectroscopic data. In vitro antimicrobial testing against five bacteria and two fungi and the prediction of activity spectra for substances (PASS) revealed that these MGP estes have promising antifungal functionality compared to their antibacterial activities. The antimicrobial tests demonstrated that the compounds 3 and 10 were the most potent against Bacillus subtilis and Escherichia coli strains, with the minimum inhibitory concentration (MIC) values ranging from 0.352 ± 0.02 to 0.703 ± 0.01 mg/ml and minimum bactericidal concentration (MBC) values ranging from 0.704 ± 0.02 to 1.408 ± 0.04 mg/ml. Density functional theory (DFT) at the B3LYP/3-21G level of theory was employed to enumerate, frontier orbital energy, enthalpy, free energy, electronic energy, MEP, dipole moment which evaluated the effect of certain groups (aliphatic and aromatic) on drug properties. They discovered that all esters were more thermodynamically stable than the parent molecule. Molecular docking is performed using AutoDock Vina to determine the binding affinities and interactions between the MGP esters and the SARS-CoV-2 main protease. The modified esters strongly interact with the prime Cys145, His41, MET165, GLY143, THR26, and ASN142 residues. The MGP esters' shape and ability to form multiple electrostatic and hydrogen bonds with the active site match other minor-groove binders' binding modes. The molecular dynamics simulation validates the molecular docking results. The pharmacokinetic characterization of the optimized inhibitor demonstrates that these MGP esters appear to be safer inhibitors and a combination of in silico ADMET (absorption, distribution, metabolism, excretion, and toxicity) prediction and drug-likeness had promising results due to their improved kinetic properties. Structure activity relationships (SAR) study including in vitro and silico results revealed that the acyl chain, palmitoyl (C16) and 4-chlorobenzoyl (4.ClC₆H₄CO-) in combination with sugar were found the most potential activates against human and fungal pathogens. After all, our comprehensive computational and statistical analysis shows that these selected MGP esters can be used as potential inhibitors against the SARS-CoV-2.

DOI： 10.1007/s10719-021-10039-3

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

A series of methyl β-D-galactopyranoside (MGP, 1) analogs were selectively acylated with cinnamoyl chloride in anhydrous N,N-dimethylformamide/triethylamine to yield 6-O-substitution products, which was subsequently converted into 2,3,4-tri-O-acyl analogs with different acyl halides. Analysis of the physicochemical, elemental, and spectroscopic data of these analogs revealed their chemical structures. In vitro antimicrobial testing against five bacteria and two fungi and the prediction of activity spectra for substances (PASS) showed promising antifungal functionality comparing to their antibacterial activities. Minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) tests were conducted for four compounds (4, 5, 6, and 9) based on their activity. MTT assay showed low antiproliferative activity of compound 9 against Ehrlich’s ascites carcinoma (EAC) cells with an IC50 value of 2961.06 µg/mL. Density functional theory (DFT) was used to calculate the thermodynamic and physicochemical properties whereas molecular docking identified potential inhibitors of the SARS-CoV-2 main protease (6Y84). A 150-ns molecular dynamics simulation study revealed the stable conformation and binding patterns in a stimulating environment. In-silico ADMET study suggested all the designed molecules to be non-carcinogenic, with low aquatic and non-aquatic toxicity. In summary, all these antimicrobial, anticancer and in silico studies revealed that newly synthesized MGP analogs possess promising antiviral activity, to serve as a therapeutic target for COVID-19.

DOI： 10.3390/molecules26227016

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI  

Lectins facilitate cell–cell contact and are critical in many cellular processes. Studying lectins may help us understand the mechanisms underlying tissue regeneration. We investigated the localization of an R-type lectin in a marine annelid (Perinereis sp.) with remarkable tissue regeneration abilities. Perinereis nuntia lectin (PnL), a galactose-binding lectin with repeating Gln-X-Trp motifs, is derived from the ricin B-chain. An antiserum was raised against PnL to specifically detect a 32-kDa lectin in the crude extracts from homogenized lugworms. The antiserum detected PnL in the epidermis, setae, oblique muscle, acicula, nerve cord, and nephridium of the annelid. Some of these tissues and organs also produced Galactose (Gal) or N-acetylgalactosamine (GalNAc), which was detected by fluorescent-labeled plant lectin. These results indicated that the PnL was produced in the tissues originating from the endoderm, mesoderm, and ectoderm. Besides, the localizing pattern of PnL partially merged with the binding pattern of a fluorescent-labeled mushroom lectin that binds to Gal and GalNAc. It suggested that PnL co-localized with galactose-containing glycans in Annelid tissue; this might be the reason PnL needed to be extracted with haptenic sugar, such as d-galactose, in the buffer. Furthermore, we found that a fluorescein isothiocyanate-labeled Gal/GalNAc-binding mushroom lectin binding pattern in the annelid tissue overlapped with the localizing pattern of PnL. These findings suggest that lectin functions by interacting with Gal-containing glycoconjugates in the tissues.

DOI： 10.3390/molecules26164799

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

In recent years, there has been considerable interest in lectins from marine invertebrates. In this study, the biological activities of a lectin protein isolated from the eggs of Sea hare (Aplysia kurodai) were evaluated. The 40 kDa Aplysia kurodai egg lectin (or AKL-40) binds to D-galacturonic acid and D-galactose sugars similar to previously purified isotypes with various molecular weights (32/30 and 16 kDa). The N-terminal sequence of AKL-40 was similar to other sea hare egg lectins. The lectin was shown to be moderately toxic to brine shrimp nauplii, with an LC50 value of 63.63 µg/mL. It agglutinated Ehrlich ascites carcinoma cells and reduced their growth, up to 58.3% in vivo when injected into Swiss albino mice at a rate of 2 mg/kg/day. The morphology of these cells apparently changed due to AKL-40, while the expression of apoptosis-related genes (p53, Bax, and Bcl-XL) suggested a possible apoptotic pathway of cell death. AKL-40 also inhibited the growth of human erythroleukemia cells, probably via activating the MAPK/ERK pathway, but did not affect human B-lymphoma cells (Raji) or rat basophilic leukemia cells (RBL-1). In vitro, lectin suppressed the growth of Ehrlich ascites carcinoma and U937 cells by 37.9% and 31.8%, respectively. Along with strong antifungal activity against Talaromyces verruculosus, AKL showed antibacterial activity against Staphylococcus aureus, Shigella sonnei, and Bacillus cereus whereas the growth of Escherichia coli was not affected by the lectin. This study explores the antiproliferative and antimicrobial potentials of AKL as well as its involvement in embryo defense of sea hare.

DOI： 10.3390/md19070394

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Language：English   Publishing type：Research paper (scientific journal)  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Sociedad Quimica de Mexico, A.C.  

Abstract. Thymidine is known as a progenitor of nucleosides that have significant biological activity. The widening importance of nucleoside derivatives as unrivaled potential antimicrobial and therapeutic agents has attracted contemplation to the synthesis of thymidine derivatives. In the present study, thymidine was treated with various acyl halides to produce 5ʹ-O-acyl thymidine derivatives by direct acylation method with an excellent yield. To obtain newer products for antimicrobial assessment studies, the 5ʹ-O-thymidine derivatives were further modified into three series of 3ʹ-O-acyl thymidine derivatives containing a wide variety of functionalities in a single molecular framework. The chemical structures of the newly synthesized compounds were elucidated by analyzing their physicochemical, elemental, and spectroscopic data. Additionally, the X-ray powder diffraction (XRD) of these acylated products was studied. For the computational investigation, we have selected eight synthesized thymidine derivatives, which have notable antibacterial activity, and performed molecular docking against bacterial lectin protein FimH of Escherichia coli (4XO8) to suggest a potent inhibitor against bacterial function. Molecular docking was performed using AutoDock Vina to calculate the binding affinities and interactions between the antibacterials and the FimH E. coli (4XO8). It was found that the selected thymidine derivatives have strongly interacted mainly with Tyr48, Tyr137, Asp140, Arg98, Gln133, Phe1, Asn23, Asn135, Lys76, Asp47, Ile13, and Ile52 residues. In silico pharmacokinetic properties were also predicted to search their absorption, metabolism, excretion, and toxicity. This computational examination showed that these thymidine derivatives might be used as potential inhibitors against the promising antibacterial activity for future studies.
 
Resumen. Se prepararon varios derivados 5ʹ-O-acil timidínicos por acilación directa con rendimientos excelentes que fueron transformados en tres series de derivados 3ʹ-O-acil timidínicos con una amplia variedad de funcionalidades. Estos compuestos fueron la base de un estudio de docking dirigido a la lectina bacteriana FimH de Escherichia coli (4XO8) con la finalidad de proponer un inhibidor contra esta función bacteriana.

DOI： 10.29356/jmcs.v65i2.1464

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Publishing type：Research paper (scientific journal)   Publisher：Pensoft Publishers  

Nucleoside derivatives are important therapeutic drugs and are the focal point in the ongoing search for novel, more potent drug targets. In this study, a new series of pyrimidine nucleoside i.e., uridine (<bold>1</bold>) derivatives were synthesized via direct method and evaluated for their antimicrobial potential activity. The title compound uridine (<bold>1</bold>) was treated with triphenylmethyl chloride in pyridine to give the 5´-<italic>O</italic>-(triphenylmethyl)uridine derivative (<bold>2</bold>), which was subsequently derivatized to create a series of 2´,3´-di-<italic>O</italic>-acyl analogs containing a wide variety of functionalities in a single molecular framework. <italic>In vitro</italic> antimicrobial functionality tests were determined against both human and plant pathogens by disc diffusion and food poisoned techniques. The chemical structures of the synthesized compounds were confirmed on the basis of their spectral, analytical, physicochemical data. The antimicrobial results indicated that the synthesized derivatives exhibited moderate to good antibacterial and antifungal activity; in particular, they were found to be more effective against fungal phytopathogens than against human bacterial strains. Compounds <bold>7</bold>, <bold>9</bold>, and <bold>14</bold> were of particular interest as they exhibited noteworthy antifungal and antibacterial properties. <italic>In vitro</italic> MTT assays revealed that compound <bold>9</bold> was effective against Ehrlich’s ascites carcinoma (EAC) cells, resulting in 7.12% and 1.34% cell growth inhibition at concentrations of 200 and 6.25 µg/ml, respectively. The IC₅₀ value for compound <bold>9</bold> was rather high and found to be 1956.25 µg/ml. Structure-activity relationship (SAR) studies were also conducted to predict structural and pharmacokinetic properties. The findings of this study indicate that the different uridine derivatives are potentially useful antimicrobial agents for the advancement of future pharmaceutical research.

DOI： 10.3897/pharmacia.68.e56543

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Other Link： https://pharmacia.pensoft.net/article/56543/download/xml/

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Walter de Gruyter GmbH  

<title>Abstract</title>
Nucleosides and their analogues are an important, well-established class of clinically useful medicinal agents that exhibit antiviral and anticancer activity. Thus, our research group has focused on the synthesis of new nucleoside derivatives that could be tested for their broad-spectrum biological activity. In this study, two new series of nucleoside derivatives were synthesized from uridine (<bold>1</bold>) through facile two-step reactions using the direct acylation method, affording 5’-<italic>O</italic>-acyl uridine derivatives in good yields. The isolated uridine analogs were further transformed into two series of 2’,3’-di-<italic>O</italic>-acyl derivatives bearing a wide variety of functionalities in a single molecular framework to evaluate their antimicrobial activity. The new synthesized compounds were characterized through physicochemical, elemental and spectroscopic analysis, and all were screened for their <italic>in vitro</italic> antimicrobial activity against selected human and plant pathogenic strains. The test compounds revealed moderate to good antibacterial and antifungal activities and were more effective against fungal phytopathogens than against bacterial strains, while many of them exhibited better antimicrobial activity than standard antibiotics. Minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) tests against all microorganisms were also conducted for five compounds based on their activity (<bold>6</bold>, <bold>11</bold>, <bold>13</bold>, <bold>16</bold>, and <bold>17</bold>). In addition, all the derivatives were optimized using density functional theory (DFT) B3LYP/6-31g+(d,p) calculations to elucidate their thermal and molecular orbital properties. A molecular docking study was performed using the human protein 5WS1 to predict their binding affinity and modes, and ADMET and SwissADME calculations confirmed the improved pharmacokinetic properties of the compounds. Besides, structure–activity relationship (SAR), thermogravimetric analysis (TGA), and X-ray diffraction (XRD) studies were also performed. Thus, the improvement of the bioactivity of these compounds is expected to significantly contribute to the design of more antimicrobial agents for therapeutic use in the future.

DOI： 10.2478/auoc-2021-0002

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Language：English   Publishing type：Research paper (scientific journal)  

Nucleoside analogs contribute in pharmaceutical and clinical fields as medicinal agents and approved drugs. This work focused to investigate the antimicrobial, anticancer activities, and structure-activity relationship (SAR) of cytidine and its analogs with computational studies. Microdilution was used to determine the antimicrobial activity, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of the modified analogs against human and phytopathogenic strains. Compounds (7), (10), and (14) were the most potent against Escherichia coli and Salmonella abony strains with MIC and MBC values from 0.316 ± 0.02 to 2.50 ± 0.03 and 0.625 ± 0.04 to 5.01 ± 0.06 mg/ml, respectively. The highest inhibitory activity was observed against gram-positive bacteria. Numerous analogs (10), (13), (14), and (15) exhibited good activity against the tested fungi Aspergillus niger and Aspergillus flavus. Anticancer activity of the cytidine analogs was examined through MTT colorimetric assay against Ehrlich's ascites carcinoma (EAC) tumor cells whereas compound 6 showed the maximum antiproliferative activity with an IC50 value of 1168.97 µg/ml. To rationalize this observation, their quantum mechanical and molecular docking studies have been performed against urate oxidase of A. flavus 1R51 to investigate the binding mode, binding affinity, and non-bonding interactions. It was observed that most of the analogs exhibited better binding properties than the parent drug. In silico ADMET prediction was attained to evaluate the drug-likeness properties that revealed the improved pharmacokinetic profile with lower acute oral toxicity of cytidine analogs. Based on the in vitro and in silico analysis, this exploration can be useful to develop promising cytidine-based antimicrobial drug(s). Supplementary Information: The online version contains supplementary material available at 10.1007/s40203-021-00102-0.

DOI： 10.1007/s40203-021-00102-0

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Nature Ltd  

SeviL is a recently isolated lectin found to bind to the linear saccharides of the ganglioside GM1b (Neu5Ac[Formula: see text](2-3)Gal[Formula: see text](1-3)GalNAc[Formula: see text](1-4)Gal[Formula: see text](1-4)Glc) and its precursor, asialo-GM1 (Gal[Formula: see text](1-3)GalNAc[Formula: see text](1-4)Gal[Formula: see text](1-4)Glc). The crystal structures of recombinant SeviL have been determined in the presence and absence of ligand. The protein belongs to the [Formula: see text]-trefoil family, but shows only weak sequence similarity to known structures. SeviL forms a dimer in solution, with one binding site per subunit, close to the subunit interface. Molecular details of glycan recognition by SeviL in solution were analysed by ligand- and protein-based NMR techniques as well as ligand binding assays. SeviL shows no interaction with GM1 due to steric hindrance with the sialic acid branch that is absent from GM1b. This unusual specificity makes SeviL of great interest for the detection and control of certain cancer cells, and cells of the immune system, that display asialo-GM1.

File： Kamata et al (2020) Sci Rep 10_22102.pdf

DOI： 10.1038/s41598-020-78926-7

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Publisher：Book Publisher International (a part of {SCIENCEDOMAIN} International)  

DOI： 10.9734/bpi/cpcs/v3

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

<title>Abstract</title>New treatment protocols are aiming to reduce the dose of the multitargeted tyrosine kinase inhibitor sunitinib, as sunitinib elicits many adverse effects depending on its dosage. Silurus asotus egg lectin (SAL) has been reported to enhance the incorporation of propidium iodide as well as doxorubicin into Burkitt’s lymphoma Raji cells through binding to globotriaosylceramide (Gb3) on the cell surface. The objective of this study was to examine whether SAL enhances the cytotoxic effect of sunitinib in Gb3-expressing HeLa cells. Although the treatment with SAL delayed the cell growth and enhanced the propidium iodide uptake, cell death accompanied by membrane collapse was not observed. The viability of sunitinib-treated HeLa cells was significantly reduced when the treatment occurred in combination with SAL compared to their separate usage. Sunitinib uptake significantly increased for 30 min in SAL-treated cells, and this increment was almost completely abolished by the addition of L-rhamnose, a hapten sugar of SAL, but not by D-glucose. After removal of SU from the medium, the intracellular sunitinib level in SAL-treated cells was higher than in untreated cells for 24 h, which was not observed in Gb3-deficient HeLa cells. Furthermore, we observed that SAL promoted the formation of lysosome-like structures, which are LAMP1 positive but not acidic in HeLa cells, which can trap sunitinib. Interestingly, SAL-induced vacuolation in HeLa cells was not observed in another Gb3 positive Raji cells. Our findings suggest that SAL/Gb3 interaction promoted sunitinib uptake and suppressed sunitinib excretion and that sunitinib efficiently exerted cytotoxicity against HeLa cells.

File： Sugawara et al (2020) Glycobiology.pdf

DOI： 10.1093/glycob/cwaa029

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Other Link： http://academic.oup.com/glycob/article-pdf/30/10/802/33807038/cwaa029.pdf

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

A 15-kDa lectin, termed SeviL, was isolated from Mytilisepta virgata (purplish bifurcate mussel). SeviL forms a noncovalent dimer that binds strongly to ganglio-series GM1b oligosaccharide (Neu5Acɑ2-3Galβ1-3GalNAcβ1-4Galβ1-4Glc) and its precursor, asialo-GM1 (Galβ1-3GalNAcβ1-4Galβ1-4Glc). SeviL also interacts weakly with the glycan moiety of SSEA-4 hexaose (Neu5Acα2-3Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glc). A partial protein sequence of the lectin was determined by mass spectrometry, and the complete sequence was identified from transcriptomic analysis. SeviL, consisting of 129 amino acids, was classified as an R(icin B)-type lectin, based on the presence of the QxW motif characteristic of this fold. SeviL mRNA is highly expressed in gills and, in particular, mantle rim tissues. Orthologue sequences were identified in other species of the family Mytilidae, including Mytilus galloprovincialis, from which lectin MytiLec-1 was isolated and characterized in our previous studies. Thus, mytilid species contain lectins belonging to at least two distinct families (R-type lectins and mytilectins) that have a common β-trefoil fold structure but differing glycan-binding specificities. SeviL displayed notable cytotoxic (apoptotic) effects against various cultured cell lines (human breast, ovarian, and colonic cancer; dog kidney) that possess asialo-GM1 oligosaccharide at the cell surface. This cytotoxic effect was inhibited by the presence of anti-asialo-GM1 oligosaccharide antibodies. With HeLa ovarian cancer cells, SeviL showed dose- and time-dependent activation of kinase MKK3/6, p38 MAPK, and caspase-3/9. The transduction pathways activated by SeviL via the glycosphingolipid oligosaccharide were triggered apoptosis. DATABASE: Nucleotide sequence data have been deposited in the GenBank database under accession numbers MK434191, MK434192, MK434193, MK434194, MK434195, MK434196, MK434197, MK434198, MK434199, MK434200, and MK434201.

File： Fuii et al (2020) FEBS J 287 2612-2630.pdf

DOI： 10.1111/febs.15154

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/febs.15154

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Part of collection (book)   Publisher：Springer US  

In the 2010s, a novel lectin family with β-trefoil folding has been identified in marine mussels from the family Mytilidae (phylum Mollusca). "MytiLec-1," the lectin described in this chapter, was the first member of this family to be isolated and characterized from the Mediterranean mussel Mytilus galloprovincialis, a commercially and ecologically important species, spread in marine coastal areas worldwide. MytiLec-1 bound to the sugar moiety of globotriose (Gb3: Galα1-4Galβ1-4Glc), an α-galactoside, leading to apoptosis of Gb3-expressing Burkitt's lymphoma cells. Although the primary structure of MytiLec-1 was quite unusual, its three-dimensional structure was arranged as a β-trefoil fold, which is the typical architecture of "Ricin B chain (or R)-type" lectins, which are found in a broad range of organisms. To date, MytiLec-1-like lectins have been exclusively found in a few species of the mollusk family Mytilidae (M. galloprovincialis, M. trossulus, M. californianus, and Crenomytilus grayanus) and in the phylum Brachiopoda. Transcriptome data revealed the presence of different structural forms of mytilectin in mussels, which included prototype and chimera-type proteins. The primary sequence of these lectins did not match any previously described known protein family, leading to their assignment to the new "mytilectin family." We here report the method of purification of this lectin and describe its use in cell biology.

DOI： 10.1007/978-1-0716-0430-4_21

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Sift Desk  

DOI： 10.25177/jccmm.4.4.ra.10663

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Publisher：MDPI  

DOI： 10.3390/books978-3-03921-821-9

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

MytiLec-1, a 17 kDa lectin with β-trefoil folding that was isolated from the Mediterranean mussel (Mytilus galloprovincialis) bound to the disaccharide melibiose, Galα(1,6) Glc, and the trisaccharide globotriose, Galα(1,4) Galβ(1,4) Glc. Toxicity of the lectin was found to be low with an LC50 value of 384.53 μg/mL, determined using the Artemia nauplii lethality assay. A fluorescence assay was carried out to evaluate the glycan-dependent binding of MytiLec-1 to Artemia nauplii. The lectin strongly agglutinated Ehrlich ascites carcinoma (EAC) cells cultured in vivo in Swiss albino mice. When injected intraperitoneally to the mice at doses of 1.0 mg/kg/day and 2.0 mg/kg/day for five consecutive days, MytiLec-1 inhibited 27.62% and 48.57% of cancer cell growth, respectively. Antiproliferative activity of the lectin against U937 and HeLa cells was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in vitro in RPMI-1640 medium. MytiLec-1 internalized into U937 cells and 50 μg/mL of the lectin inhibited their growth of to 62.70% whereas 53.59% cell growth inhibition was observed against EAC cells when incubated for 24 h. Cell morphological study and expression of apoptosis-related genes (p53, Bax, Bcl-X, and NF-κB) showed that the lectin possibly triggered apoptosis in these cells.

DOI： 10.3390/md17090502

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Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier {BV}  

DOI： 10.1016/j.marpolbul.2019.02.061

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier {BV}  

DOI： 10.1016/j.ijbiomac.2018.11.222

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

We identified a lectin (carbohydrate-binding protein) belonging to the complement 1q(C1q) family in the feather star Anneissia japonica (a crinoid pertaining to the phylum Echinodermata). The combination of Edman degradation and bioinformatics sequence analysis characterized the primary structure of this novel lectin, named OXYL, as a secreted 158 amino acid-long globular head (sgh)C1q domain containing (C1qDC) protein. Comparative genomics analyses revealed that OXYL pertains to a family of intronless genes found with several paralogous copies in different crinoid species. Immunohistochemistry assays identified the tissues surrounding coelomic cavities and the arms as the main sites of production of OXYL. Glycan array confirmed that this lectin could quantitatively bind to type-2 N-acetyllactosamine (LacNAc: Galβ1-4GlcNAc), but not to type-1 LacNAc (Galβ1-3GlcNAc). Although OXYL displayed agglutinating activity towards Pseudomonas aeruginosa, it had no effect on bacterial growth. On the other hand, it showed a significant anti-biofilm activity. We provide evidence that OXYL can adhere to the surface of human cancer cell lines BT-474, MCF-7, and T47D, with no cytotoxic effect. In BT-474 cells, OXYL led to a moderate activation of the p38 kinase in the MAPK signaling pathway, without affecting the activity of caspase-3. Bacterial agglutination, anti-biofilm activity, cell adhesion, and p38 activation were all suppressed by co-presence of LacNAc. This is the first report on a type-2 LacNAc-specific lectin characterized by a C1q structural fold.

DOI： 10.3390/md17020136

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Acta Pharmaceutica Sciencia  

DOI： 10.23893/1307-2080.aps.05704

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Forum: Carbohydrates Coming of Age  

DOI： 10.4052/tigg.1717.1j

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Authorship：Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

File： Fujii et al TIGG 30 177J155-188_1717.1J.pdf

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DOI： 10.1186/s12864-017-4012-z

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DOI： 10.1038/s41598-017-06332-7

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DOI： 10.1111/jth.13617

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File： Fujii et al (2017) Glycoconj J 34 85-94.pdf

DOI： 10.1007/s10719-016-9731-x

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File： Sugawara et al (2017) Glycoconj J 34 127-138.pdf

DOI： 10.1007/s10719-016-9739-2

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DOI： 10.1002/elps.201600346

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File： Ch33 Hasan et al Marine Glycobiology Principles and Applications KIM 2016 CRC.pdf

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DOI： 10.1038/srep28344

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DOI： 10.3390/md14050092

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File： Kabir et al (2016) Int J Biol Macromol 84 62-68.pdf

DOI： 10.1016/j.ijbiomac.2015.12.006

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File： Kamitori et al (2016) Regenerative Therapy 3 11-14.pdf

DOI： 10.1016/j.reth.2016.01.006

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：{SciPress} Ltd  

<jats:p>A novel series of benzenesulfonyl derivatives of methyl α-D-glucopyranoside (1) were synthesized by reacting benzenesulfonyl chloride in pyridine followed by direct acylation method to yield 6-<jats:italic>O</jats:italic>-benzenesulfonyl derivative (2). In order to obtain newer products for antibacterial evaluation studies, the 6-<jats:italic>O</jats:italic>-benzenesulfonyl derivative was further transformed to a series of 2,3,4-tri-<jats:italic>O</jats:italic>-acyl derivatives (3-11) containing a wide variety of functionalities in a single molecular framework. All the synthesized compounds have been confirmed by IR,<jats:sup>1</jats:sup>H NMR and elemental analysis. These newly synthesized compounds were screened for<jats:italic>in vitro</jats:italic>antibacterial activity against some human pathogenic bacterial strains. The study revealed that the acylated products exhibit moderate to good antibacterial activities. It was interesting to observe that the selected compounds were more sensitive against Gram-Ve bacteria than that of the Gram-+Ve bacterial strains.</jats:p>

File： Kawsar et al (2016) Int Lett Chem Phys Astro 64 95-105.pdf

DOI： 10.18052/www.scipress.com/ilcpa.64.95

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Growing Science  

File： Chowdhury et al (2016) Curr Chem Lett 5 83-92.pdf

DOI： 10.5267/j.ccl.2015.12.001

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Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

DOI： 10.3390/molecules21010129

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Scientific Research Publishing, Inc.  

File： Kawsar et al (2015) Int J Org Chem USA 5(4) 232-245.pdf

DOI： 10.4236/ijoc.2015.54023

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File： Hasan et al (2015) Mar Drugs 13(12)7377-7389.pdf

DOI： 10.3390/md13127071

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

Toward the development of ecotoxicology methods to investigate microbial markers of impacts of hydrocarbon processing activities, DNA adductomic analyses were conducted on a sphingomonad soil bacterium. From growing cells that were exposed or unexposed to acrolein, a commonly used biocide in hydraulic fracturing processes, DNA was extracted, digested to 2'-deoxynucleosides and analyzed by liquid chromatography-positive ionization electrospray-tandem mass spectrometry in selected reaction monitoring mode transmitting the [M + H](+) &gt; [M + H - 116](+) transition over 100 transitions. Overall data shown as DNA adductome maps revealed numerous putative DNA adducts under both conditions with some occurring specifically for each condition. Adductomic analyses of triplicate samples indicated that elevated levels of some targeted putative adducts occurred in exposed cells. Two exposure-specific adducts were identified in exposed cells as 3-(2'-deoxyribosyl)-5,6,7,8-tetrahydro-6-hydroxy- (and 8-hydroxy-)pyrimido[1,2-a]-purine-(3H)-one (6- and 8-hydroxy-PdG) following synthesis of authentic standards of these compounds and subsequent analyses. A time course experiment showed that 6- and 8-hydroxy-PdG were detected in bacterial DNA within 30 min of acrolein exposure but were not detected in unexposed cells. This work demonstrated the first application of DNA adductomics to examine DNA damage in a bacterium and sets a foundation for future work.

DOI： 10.1002/mbo3.283

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：{SciPress} Ltd  

File： Kawsar et al (2015) Int Lett Chem Phys Astro 58 122-136.pdf

DOI： 10.18052/www.scipress.com/ilcpa.58.122

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DOI： 10.1099/ijs.0.000356

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DOI： 10.1007/s10532-015-9739-0

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DOI： 10.1002/mbo3.283

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File： Kawsar et al (2015) Current Res Chem 7(2), 21-33.pdf

DOI： 10.3923/crc.2015.21.33

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File： Koide et al (2015) Ann. Marine Biol. Res. 2, 1005.pdf

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCI LTD  

Comprehensive analyses of the biotransformation of the N-heteroaromatic environmental pollutant, indole, by a newly isolated soil bacterium designated strain KK10, were conducted by liquid chromatography negative electrospray ionization tandem mass spectrometry (LC/ESI(-)-MS/MS). Numerous indole bioproducts were revealed and provided evidence for multiple pathways of indole biotransformation by this strain including unreported pathways. Oxidation of the N-heterocyclic ring of indole and ring cleavage through N-formylanthranilic acid and gentisic acid was characterized in addition to a carbocyclic ring-cleavage pathway which was documented for the first time. Three carbocyclic aromatic ring cleavage products of indole were proposed and the structure of 2,3-pyrrole-dicarboxylic acid was confirmed following synthesis and analyses of an authentic standard of this compound. Eight downstream ring-fission products were identified overall and these results confirmed the lower pathways of indole biotransformation. Indigo, indirubin and other indigoid compounds were produced by strain KK10 and confirmed N-heterocyclic-ring oxidations in the upper pathways. Nearly complete sequencing of the 16S rRNA gene of strain KK10 and phylogenetic analysis revealed that it was a new member of the genus Cupriavidus. Cupriavidus strains that biotransform N-heteroaromatic pollutants have not been described. These results expand both our understanding of the versatile catabolic capabilities of members of the genus Cupriavidus and provide evidence for alternative biotransformation mechanisms for indole in the environment by bacteria. (C) 2014 Elsevier Ltd. All rights reserved.

File： Fukuoka et al (2015) Int Biodetrio Biodegra 97 13-24.pdf

DOI： 10.1016/j.ibiod.2014.11.007

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCI LTD  

Comprehensive analyses of the biotransformation of the N-heteroaromatic environmental pollutant, indole, by a newly isolated soil bacterium designated strain KK10, were conducted by liquid chromatography negative electrospray ionization tandem mass spectrometry (LC/ESI(-)-MS/MS). Numerous indole bioproducts were revealed and provided evidence for multiple pathways of indole biotransformation by this strain including unreported pathways. Oxidation of the N-heterocyclic ring of indole and ring cleavage through N-formylanthranilic acid and gentisic acid was characterized in addition to a carbocyclic ring-cleavage pathway which was documented for the first time. Three carbocyclic aromatic ring cleavage products of indole were proposed and the structure of 2,3-pyrrole-dicarboxylic acid was confirmed following synthesis and analyses of an authentic standard of this compound. Eight downstream ring-fission products were identified overall and these results confirmed the lower pathways of indole biotransformation. Indigo, indirubin and other indigoid compounds were produced by strain KK10 and confirmed N-heterocyclic-ring oxidations in the upper pathways. Nearly complete sequencing of the 16S rRNA gene of strain KK10 and phylogenetic analysis revealed that it was a new member of the genus Cupriavidus. Cupriavidus strains that biotransform N-heteroaromatic pollutants have not been described. These results expand both our understanding of the versatile catabolic capabilities of members of the genus Cupriavidus and provide evidence for alternative biotransformation mechanisms for indole in the environment by bacteria. (C) 2014 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.ibiod.2014.11.007

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Academic Journals Inc.  

File： Kawsar 2015 Int J Biol Chem 9(6) 269-282.pdf

DOI： 10.3923/ijbc.2015.269.282

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Publishing type：Research paper (scientific journal)   Publisher：The Society of Japanese Women Scientists  

DOI： 10.5939/sjws.15006

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DOI： 10.1007/s10695-014-9957-0

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER  

Rhamnose-binding lectin (RBL) is one of the animal lectin categories which take part in the innate immune responses of fish. Osmerus lanceolatus lectin (OLL) from shishamo smelt eggs is an RBL composed of two tandem-repeated domains, both of which are considered to be a carbohydrate-recognition domain. SAL, catfish (Silurus asotus) egg RBL composed of three domains, binds to Burkitt's lymphoma Raji cells through globotriaosylceramide (Gb3) carbohydrate chain and to reduce cell size and growth by altering membrane composition without causing cell death. In this experiment, we tried to compare the binding effects of these two RBLs on Raji cells. Flow cytometric and fluorescence microscopic analyses revealed that OLL also directly bound to and shrunk Raji cells with ten times less reactivity than SAL but reduced cell growth with decreasing cell viability. Anti-Gb3 antibody completely blocked the binding of SAL to Raji cells but not that of OLL. In addition, the direct bindings of OLL and SAL to Raji cells were comparably inhibited by melibiose, but lactose was more effective inhibitor for the binding of OLL than that of SAL. These results suggest that OLL has slightly different cell-binding property compared with SAL and binds not only to Gb3 but also to the other carbohydrate receptor-bearing beta-galactoside chains. The quantitative RT-PCR analysis revealed that SAL induced the expression of TNF-alpha but not of IFN-gamma, IL-1 beta, and IL-10. Thus, SAL-induced cytostatic effect on Raji cells might be partially caused by TNF-alpha-mediated signaling pathway.

DOI： 10.1007/s10695-014-9948-1

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Language：English   Publishing type：Research paper (scientific journal)  

Rhamnose-binding lectin (RBL) is one of the animal lectin categories which take part in the innate immune responses of fish. Osmerus lanceolatus lectin (OLL) from shishamo smelt eggs is an RBL composed of two tandem-repeated domains, both of which are considered to be a carbohydrate-recognition domain. SAL, catfish (Silurus asotus) egg RBL composed of three domains, binds to Burkitt's lymphoma Raji cells through globotriaosylceramide (Gb3) carbohydrate chain and to reduce cell size and growth by altering membrane composition without causing cell death. In this experiment, we tried to compare the binding effects of these two RBLs on Raji cells. Flow cytometric and fluorescence microscopic analyses revealed that OLL also directly bound to and shrunk Raji cells with ten times less reactivity than SAL but reduced cell growth with decreasing cell viability. Anti-Gb3 antibody completely blocked the binding of SAL to Raji cells but not that of OLL. In addition, the direct bindings of OLL and SAL to Raji cells were comparably inhibited by melibiose, but lactose was more effective inhibitor for the binding of OLL than that of SAL. These results suggest that OLL has slightly different cell-binding property compared with SAL and binds not only to Gb3 but also to the other carbohydrate receptor-bearing β-galactoside chains. The quantitative RT-PCR analysis revealed that SAL induced the expression of TNF-α but not of IFN-γ, IL-1β, and IL-10. Thus, SAL-induced cytostatic effect on Raji cells might be partially caused by TNF-α-mediated signaling pathway.

File： Hosono et al (2014) Fish Physiol Biochem 40 1559-1572.pdf

DOI： 10.1007/s10695-014-9948-1

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File： Hasan et al (2014) Molecules 19_13990-14003.pdf

DOI： 10.3390/molecules190913990

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File： Hasan et al (2014) Indian J Biochem Biophys 51(2) 142-148.pdf

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File： Hasan et al (2014) African J Biotechnol 13(15) 1679-1685.pdf

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY  

A pathway for the biotransformation of the environmental pollutant and high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by a soil bacterium was constructed through analyses of results from liquid chromatography negative electrospray ionization tandem mass spectrometry (LC/ESI(-)-MS/MS). Exposure of Sphingobium sp. strain KK22 to benzo[k]fluoranthene resulted in transformation to four-, three- and two-aromatic ring products. The structurally similar four- and three-ring non-alternant PAHs fluoranthene and acenaphthylene were also biotransformed by strain KK22, and LC/ESI(-)-MS/MS analyses of these products confirmed the lower biotransformation pathway proposed for benzo[k]fluoranthene. In all, seven products from benzo[k]fluoranthene and seven products from fluoranthene were revealed and included previously unreported products from both PAHs. Benzo[k]fluoranthene biotransformation proceeded through ortho-cleavage of 8,9-dihydroxy-benzo[k]fluoranthene to 8-carboxyfluoranthenyl-9-propenic acid and 9-hydroxy-fluoranthene-8-carboxylic acid, and was followed by meta-cleavage to produce 3-(2-formylacenaphthylen-1-yl)-2-hydroxy-prop-2-enoic acid. The fluoranthene pathway converged with the benzo[k]fluoranthene pathway through detection of the three-ring product, 2-formylacenaphthylene-1-carboxylic acid. Production of key downstream metabolites, 1,8-naphthalic anhydride and 1-naphthoic acid from benzo[k]fluoranthene, fluoranthene and acenaphthylene biotransformations provided evidence for a common pathway by strain KK22 for all three PAHs through acenaphthoquinone. Quantitative analysis of benzo[k]fluoranthene biotransformation by strain KK22 confirmed biodegradation. This is the first pathway proposed for the biotransformation of benzo[k]fluoranthene by a bacterium.

DOI： 10.1111/1751-7915.12102

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File： 2013 Maeda Kanaly et al Microbial Biotechology.pdf

DOI： 10.1111/1751-7915.12102

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File： Ogawa et al (2014) Glycoconj J 31(2) 171-184.pdf

DOI： 10.1007/s10719-013-9513-7

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Osterreichische Apotheker-Verlagsgesellschaft m.b.H.  

File： Kawsar et al (2014) Sci Pharm 82 1-20.pdf

DOI： 10.3797/scipharm.1308-03

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Osmerus (Spirinchus) lanceolatus egg lectin (OLL) is a member of the rhamnose-binding lectin (RBL) family which is mainly found in aqueous beings. cDNA of OLL was cloned, and its genomic architecture was revealed. The deduced amino acid (aa) sequence indicated that OLL was composed of 213 aa including 95 aa of domain N and 97 aa of domain C. N and C showed 73 % sequence identity and contained both -ANYGR- and -DPC-KYL-peptide motifs which are conserved in most of the RBL carbohydrate recognition domains. The calculated molecular mass of mature OLL was 20,852, consistent with the result, and 20,677.716, from mass spectrometry. OLL was encoded by eight exons: exons 1 and 2 for a signal peptide; exons 3-5 and 6-8 for N- and C-domains, respectively. Surface plasmon resonance spectrometric analyses revealed that OLL showed comparable affinity for Galα- and β-linkages, whereas Silurus asotus lectin (SAL), a catfish RBL, bound preferentially to α-linkages of neoglycoproteins. The Kd values of OLL and SAL against globotriaosylceramide (Gb3) were 1.69 × 10⁻⁵ M for and 2.81 × 10⁻⁶ M, respectively. Thus, the carbohydrate recognition property of OLL is slightly different from that of SAL. On the other hand, frontal affinity chromatography revealed that both OLL and SAL interacted with only glycolipid-type oligosaccharides such as Gb3 trisaccharides, not with N-linked oligosaccharides. The domain composition of these RBLs and an analytical environment such as the "cluster effect" of a ligand might influence the binding between RBL and sugar chains.

File： Hosono et al (2013) Fish Physiol Biochem 39 1619-1630.pdf

DOI： 10.1007/s10695-013-9814-6

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File： Kawsar et al (2013) Hacettepe J Biol Chem 41(3) 195-206.pdf

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File： Kunihiro et al (2013) Appl Environ Microbiol 79 4410.pdf

DOI： 10.1128/AEM.01129-13

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Language：English   Publishing type：Part of collection (book)   Publisher：John Wiley and Sons  

File： Ozeki et al (2013) Chapt 8 Marine Proteins & Peptides Wiley & Blackwell.pdf

DOI： 10.1002/9781118375082.ch8

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File： Yasumitsu et al (2013) Protein Pep Lett 20 213-217.pdf

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File： Maeda et al Kanaly e00911-13.full.pdf

DOI： 10.1128/genomeA.00911-13

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File： Fujii et al (2012) J Biol Chem 287 44772-44783.pdf

DOI： 10.1074/jbc.M112.418012

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File： Yamamoto et al Biochemistry(2012) 51 5329-5338.pdf

DOI： 10.1021/bi300442c

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File： Matsumoto et al (2012) Toxins 4 323-338.pdf

DOI： 10.3390/toxins4050323

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File： Fujii et al (2012) Protein J 31,(1) 15-26.pdf

DOI： 10.1007/s10930-011-9369-2

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File： Yasumitsu et al (2011) In Vitro Cell Dev Biol 47, 728-734.pdf

DOI： 10.1007/s11626-011-9462-z

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File： Kawsar et al (2011) Protein Journal 30 509-519.pdf

DOI： 10.1007/s10930-011-9356-7

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File： Matsumoto et al (2011) CBP 158 266-273.pdf

DOI： 10.1016/j.cbpb.2010.12.004

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File： Fujii et al CBP 158 30-37 (2011).pdf

DOI： 10.1016/j.cbpb.2010.09.002

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File： Kawsar et al (2011) Phytologia Balcanica 17, 347-354.pdf

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File： Yasumitsu et al (2010) Anal Biochem 406 86-88.pdf

DOI： 10.1016/j.ab.2010.06.035

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File： Kawsar et al (2010)Int J Biol Life Sci 6 31-37.pdf

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File： Yasumitsu et al (2010) Electrophoresis 31 1913-1917.pdf

DOI： 10.1002/elps.200900524

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DOI： 10.2174/092986610791112648

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File： Kawsar et al (2010)Int J Biol Life Sci 6 44-50.pdf

DOI： 10.5281/ZENODO.1057039

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File： Kawsar et al (2010) Arch Biol Sci Belgrade 62 1927-1038.pdf

DOI： 10.2298/ABS1004027K

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File： Kawsar et al (2009) Biochemistry(Moscow)74, 709-716.pdf

DOI： 10.1134/S0006297909070025

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産業総合研究所糖鎖医工学研究センターのレクチンデータベース構築のために提供したウシガエル卵ガレクチンの糖鎖結合プロファイルは、他のβガラクトシド結合性ガレクチンと異なり、キノコやカイメン由来のガレクチン同様に血液型A型やフォルスマン抗原などN-アセチルガラクトサミンンを特異的に認識し、細胞接着も行うことが見いだせられました

File： Kawsar et al (2009) PPL 16,677-684.pdf

DOI： 10.2174/092986609788490104

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File： Kawsar et al (2009) CBP 152, 382-389.pdf

DOI： 10.1016/j.cbpb.2009.01.009

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DOI： 10.2174/092986609788490104

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DOI： 10.3923/ijbc.2009.42.48

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File： Fujii et al (2009) J Biol Sci 9 (4) 319-325.pdf

DOI： 10.3923/jbs.2009.319.325

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE INC  

File： Kawsar et al (2008) CBP 150 pp349-357.pdf

DOI： 10.1016/j.cbpb.2008.04.004

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE INC  

DOI： 10.1016/j.cbpb.2008.04.004

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3923/jbs.2008.1051.1056

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DOI： 10.3923/ajpp.2008.165.172

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE INC  

Galactoside-binding lectin was purified from the snake venom of Crotalus ruber by affinity chromatography on a lactose-agarose column, and the complete amino acid sequence was determined. The C. ruber venom lectin (CRL) showed a single band of 28 kDa by SDS-polyacrylamide electrophoresis under non-reducing conditions, but it showed a single band of 15 kDa under reducing conditions, indicating that CRL is a disulfide-linked homodimer of 15 kDa subunit. CRL specifically recognized beta-galactosides such as thiodigalactoside followed by Nacetylgalactosamine when examined with their inhibitory effects on CPL-induced hemagglutination. A CRL subunit was composed of 135 residues containing nine Cys residues and showed a high similarity to other C-type galactoside-binding lectins from snake venoms. C. atrox lectin (CAL) showed almost the same sequence except for eight amino acid residues. Neither CRL nor CAL induced platelet aggregation by itself or inhibited platelet aggregation mediated by von Willebrand factor or fibrinogen with agonists. CRL showed a similar oligomeric form and the sugar specificity as CAL, but it showed different divalent cation sensitivity such as Mn2+ and Ni2+. Homology modeling suggested that the amino acid substitution found in CRL does not affect sugar recognition of the lectin but might alter the conformation and influence the sugar binding pocket induced by the metal-ion binding. (c) 2006 Elsevier Inc. All rights reserved.

File： Hamako et al (2007) Comp Biochem Physiol partB146 299-306.pdf

DOI： 10.1016/j.cbpb.2006.11.022

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE INC  

Galactoside-binding lectin was purified from the snake venom of Crotalus ruber by affinity chromatography on a lactose-agarose column, and the complete amino acid sequence was determined. The C. ruber venom lectin (CRL) showed a single band of 28 kDa by SDS-polyacrylamide electrophoresis under non-reducing conditions, but it showed a single band of 15 kDa under reducing conditions, indicating that CRL is a disulfide-linked homodimer of 15 kDa subunit. CRL specifically recognized beta-galactosides such as thiodigalactoside followed by Nacetylgalactosamine when examined with their inhibitory effects on CPL-induced hemagglutination. A CRL subunit was composed of 135 residues containing nine Cys residues and showed a high similarity to other C-type galactoside-binding lectins from snake venoms. C. atrox lectin (CAL) showed almost the same sequence except for eight amino acid residues. Neither CRL nor CAL induced platelet aggregation by itself or inhibited platelet aggregation mediated by von Willebrand factor or fibrinogen with agonists. CRL showed a similar oligomeric form and the sugar specificity as CAL, but it showed different divalent cation sensitivity such as Mn2+ and Ni2+. Homology modeling suggested that the amino acid substitution found in CRL does not affect sugar recognition of the lectin but might alter the conformation and influence the sugar binding pocket induced by the metal-ion binding. (c) 2006 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.cbpb.2006.11.022

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/S0197-0186(02)00092-X

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File： Matsui et al (2001) Biochem Biophys Acta 1525 50-57.pdf

DOI： 10.1016/S0304-4165(00)00170-7

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Language：English   Publishing type：Research paper (scientific journal)  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley-Blackwell  

File： Ozeki (1998) IUBMB Life 45 989-995.pdf

DOI： 10.1002/iub.7510450516

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Language：English   Publishing type：Research paper (scientific journal)  

File： Uchiyama et al (1997) Glycobiology 7 1159-1165.pdf

DOI： 10.1093/glycob/7.8.1159

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Language：English  

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File： Ozeki et al (1997) CBP 118B 1-6.pdf

DOI： 10.1016/S0305-0491(97)00014-X

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Language：English   Publishing type：Research paper (scientific journal)  

File： Ozeki Y (1997) IUBMB Life 41(3) 633-640.pdf

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File： Matsui et al (1997) J Biochem121 376-381.pdf

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPANESE BIOCHEMICAL SOC  

The binding of human von Willebrand factor (vWF) to a variety of extracellular matrix components immobilized on plates and the binding of vWF to platelet glycoprotein It, (GPIb) after interacting with these matrix components mere examined by means of an enzyme-linked immunosorbent assay. vWF preferably bound to type III collagen, whereas it did not significantly bind to type I, IV, V, or VI collagen, fibronectin, laminin, elastin, or proteoglycans. Soluble type III collagen did not bind to vWF coated on plates and showed a little effect on the vWF binding to the immobilized collagen, suggesting that solid-phase collagen is important for the interaction with vWF. When glycocalicin, the N-terminal carbohydrate-rich extracellular domain of GPIb alpha exhibiting the vWF-binding activity, was added to vWF bound to collagen type III, no significant binding of glycocalicin was observed, but it bound to vWF in the presence of botrocetin, a vWF modulator protein isolated from Bothrops jararaca snake venom. These results indicate that vWP immobilized on collagen can interact with GPIb but that the binding of vWF to the collagen matrix alone is insufficient for modulating vWF so that it interacts with GPIb under static conditions. Another unknown physiological modulator functionally mimicking botrocetin or high-shear stress may be involved in the platelet adhesion to extracellular mat-fix in the early stage of hemostasis.

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Language：English   Publishing type：Research paper (scientific journal)  

File： Kou et al J Clin Invest 98(12)2813-2813.pdf

DOI： 10.1172/JCI119109

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1006/bbrc.1996.1345

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Publishing type：Research paper (scientific journal)  

DOI： 10.1016/0305-0491(95)00121-2

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File： Hamako et al (1993) Comp Biochem Physiol 106B 949-954.pdf

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Language：English   Publishing type：Research paper (scientific journal)  

File： Ozeki et al (1995) Glycobiology 5, 255-261.pdf

DOI： 10.1093/glycob/5.2.255

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Language：English   Publishing type：Research paper (scientific journal)  

File： Ozeki et al (1995) Exp Cell Res 216, 318-324.pdf

DOI： 10.1006/excr.1995.1040

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Language：English   Publishing type：Research paper (scientific journal)  

File： Ozeki et al (1994) Arch Biochem Biophys 308(1) 306-310.pdf

DOI： 10.1006/abbi.1994.1043

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press  

File： Matsui et al (1994) J Biochem116 1127-1133.pdf

DOI： 10.1093/oxfordjournals.jbchem.a124638

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

1. Asparagine-linked sugar chains released by hydrazinolysis from IgGs of porcine, equine, bovine, goat, ovine, canine, rabbit, guinea-pig and rat were comparatively analyzed by microsequencing and lectin affinity chromatography.
2. Sugar chains of all IgGs basically consisted of biantennary complex-type oligosaccharides containing 0-2 sialic acid residue(s). More than 70% of the oligosaccharides were neutral, except for guinea-pig IgG, and fucosylated trimannosyl core structures were dominant except for rabbit IgG. Bisecting N-acetylglucosamine residue was absent in porcine and equine IgGs.
3. A large quantity of galactose-less oligosaccharides were present in IgGs of porcine, equine, canine and rat.

DOI： 10.1016/0305-0491(93)90056-B

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier {BV}  

File： Hamako et al (1993) Comp Biochem Physiol 106B 949-954.pdf

DOI： 10.1016/0305-0491(93)90056-b

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Language：English   Publishing type：Research paper (scientific journal)  

File： Usami et al (1993) PNAS 90 928-932.pdf

DOI： 10.1073/pnas.90.3.928

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/0014-5793(91)81055-D

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File： Ozeki et al (1991) BBRC 178 404-413.pdf

DOI： 10.1016/0006-291X(91)91828-Z

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Language：English   Publishing type：Research paper (scientific journal)  

File： Ozeki et al (1991) Biochemistry 30 2391-2394.pdf

DOI： 10.1021/bi00223a014

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Language：English   Publishing type：Research paper (scientific journal)  

File： Hattori et al (1990) J. J. Breed 40, 295-301.pdf

DOI： 10.1270/jsbbs1951.40.295

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Total pages：540   Responsible for pages：407-418   Language：English   Book type：Scholarly book

ASIN

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Total pages：744   Responsible for pages：407-418   Language：English   Book type：Scholarly book

ASIN

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Total pages：816   Responsible for pages：167-184   Language：English   Book type：Scholarly book

ASIN

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Language：English   Presentation type：Oral presentation (general)  

Venue：Qingdao, China  

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File： 2018_June_09 CGM.jpg

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Language：English   Presentation type：Oral presentation (keynote)  

Venue：Mohali, Punjab, India  

File： Final Program of CARBO-XXIX.pdf

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Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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Language：English   Presentation type：Oral presentation (invited, special)  

Venue：インド共和国 マハーラーシュトラ州 ムンバイ大学  

File： Final Program 102nd ISC.pdf

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Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

File： 2012年3月6日ライフイノベーションのための糖鎖関連シーズ神奈川県受託事業講演.pdf

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Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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Language：English   Presentation type：Oral presentation (general)  

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Language：English   Presentation type：Poster presentation  

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Language：English   Presentation type：Poster presentation  

Venue：Honolulu, Hawaii  

File： Hasan et al (2014) Glycobiology 24 1053-1231 Conference abstract.pdf

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

Venue：長崎県佐世保市  

File： 2014年9月11日長崎国際大学要旨大関泰裕.pdf

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Language：English   Presentation type：Oral presentation (invited, special)  

Venue：Bengaluru, The Republic of India  

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File： 2019-06-08_.jpg

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Language：English   Presentation type：Oral presentation (general)  

Venue：Baltimore, Maryland, USA  

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Presentation type：Oral presentation (invited, special)  

Venue：秋葉原コンベンションホール５階カンファレンスフロア/５B会議室  

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Presentation type：Symposium, workshop panel (nominated)  

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Other Link： https://www.ncbi.nlm.nih.gov/books/NBK579951/

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DOI： 10.1101/9781621824213

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File： 第23回比較グライコーム研究会2021ポスターfinal.pdf

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Other Link： https://www.yokohama-cu.ac.jp/english/news/2022/20220323_23rd-glycomeh.html

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Collaboration of lectin researches with crystallographers

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Other Link： https://www2.chubu.ac.jp/digibook/wpwt/216/html5.html#page=15

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File： onlineシンポ2020年8月21日比較グライコーム研究会(6_05版).pdf

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Other Link： http://www-user.yokohama-cu.ac.jp/~english/index.php/2020/06/11/a-ycu-alumnus-prof-dr-s-m-abe-kawsar-university-of-chittagong-bangladesh-published-two-academic-books-from-a-german-publisher/

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File： 2014 - Ozeki - YCU, San Diego retreat program - SA_rev2.pdf

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Work type：Educational material   Location：Yokohama City University  

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