記述言語：英語   掲載種別：研究論文（学術雑誌）  

To characterize and compare macular vessel density in central retinal artery occlusion (CRAO) eyes with retinal arterial cannulation and CRAO eyes with standard treatment. This study was Cross-sectional, observational study. Twenty-two eyes with nonarteric CRAO which underwent retinal arterial cannulation and 19 eyes with nonarteric CRAO with standard treatment were included. Optical coherent tomography angiography (OCTA)-based macular vessel density and visual acuity were examined. The dynamic ranged-based normalized rates of vessel density was compared within each group at the first visit to the clinic and 7 days after the onset. Macular vessel density in cannulation group was significantly better at 7 days after the onset than that at the first visit (3.73 ± 3.02 mm-1 vs. 7.89 ± 1.02 mm-1, P = 0.0001), while there wasn't significant improvement of macular vessel density in standard treatment group at 7 days after the onset (2.13 ± 1.62 mm-1 vs. 2.89 ± 0.22 mm-1, P = 0.067). At one month after the onset, mean LogMAR visual acuity in CRAO eyes with cannulation significantly improved compared with that at the first visit after the onset (1.678 vs. 0.979, P = 0.00012). Macular vessel density loss in CRAO eyes was improved by retinal arterial cannulation. Early intervention of retinal arterial cannulation is useful for minimizing visual impairment in CRAO eyes.

DOI： 10.1038/s41598-023-46267-w

PubMed

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: To customize a passive surgery support robot for ophthalmic surgery and preliminarily evaluate its performance. STUDY DESIGN: Prospective observational study. METHODS: The range of motion of the arm was analyzed during ophthalmic surgery and, based on this analysis, a commercially available passive robot was customized for surgical support for ophthalmic surgery; following which a prototype robot was constructed. To examine the effects on the brachial muscle during surgical operations with and without the prototype robot, surface electromyograms of the biceps and triceps were analyzed after performing continuous curvilinear capsulorrhexis (CCC) and suturing the sclerocorneal wound in a cataract surgery simulation. Six surgeons performed cataract surgery, and the degree of arm stability and muscle fatigue during surgery were evaluated using a visual analog scale. RESULTS: During surgery, the prototype robot enabled fixation of the elbow and wrist at any position within the surgeon's range of motion, expanding the range of motion of the hand and fingers and stabilizing operability. Surface electromyography showed a significant decrease in the mean amplitude value of the biceps brachii during both CCC and suturing (p < 0.0001). No significant difference was observed in the triceps brachii. The arm stability and muscle fatigue were improved by 83.3% on the visual analog scale with the prototype robot compared with that without protpotype robot. CONCLUSION: The use of a passive prototype robot may improve arm stability and reduce muscle fatigue during ophthalmic surgery.

DOI： 10.1007/s10384-023-01017-3

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: To evaluate the relationship between retinal haemorrhages detected on Ultra-widefield (UWF) red channel images and perfusion status in eyes with acute central retinal vein occlusion (CRVO). METHODS: UWF fundus images were split into green and red channels using ImageJ software. The retinal haemorrhages were calculated quantitatively with both the green and red channel images, resulting in green channel haemorrhages (GCH) and red channel haemorrhages (RCH). The nonperfusion area (NPA) was also calculated from fluorescein angiography in each eye. The relationships between both the GCH and RCH with the NPA were investigated. RESULTS: Thirty-two eyes of 32 patients with acute CRVO (18 men, 14 women) were included. The mean GCH and RCH values were 10.4% ± 8.2% and 1.7% ± 1.7%, respectively. The mean NPA was 39.2% ± 28.8%. Significant correlations were seen between the GCH and NPA (r = 0.38; P = 0.022) and RCH and NPA (r = 0.44; P = 0.010, linear regression analysis). Multivariate analysis suggested that only the RCHs were correlated significantly with the NPA. CONCLUSIONS: Retinal haemorrhages detected by UWF red channel imaging were less compared to green channel imaging and associated closely with retinal NPAs in eyes with acute CRVO. UWF red channel imaging allowed us to identify ischaemia-related haemorrhage.

DOI： 10.1038/s41433-022-02337-3

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: To evaluate the efficacy of anterior segment optical coherent tomography (AS OCT) in estimating the length of the pars plana and optimizing the sclerotomy entry site in vitrectomy for highly myopic eyes, facilitating membrane peeling. METHODS: Twenty-three eyes with myopic traction maculopathy were studied. The pars plana was examined using two methods: preoperative AS OCT and intraoperative measurement. The distance from the limbus to the ora serrata in two groups was measured to compare differences in length. The actual length of the entry site from the limbus and forceps used were noted in all eyes studied. RESULTS: The mean axial length was 29.2 ± 2.3 mm for all 23 eyes. The average length between the limbus and ora serrata measured with AS OCT and intraoperative examination was 6,710 µ m (SD ± 459) and 6,671 µ m (SD ± 402), respectively, in the superotemporal region ( P > 0.05), and 6,340 µ m (SD ± 321) and 6,204 µ m (SD ± 402), respectively, in the superonasal region ( P >0.05). The mean length of the entry site from the limbus was 6.2 mm, and 28-mm forceps were used in 17 of 23 eyes (77%). CONCLUSION: The length of the pars plana varies depending on the axial length of the eye. Preoperative AS OCT enables accurate measurement of the pars plana in eyes with high myopia. AS OCT examination can help determine the optimal site for sclerotomy, allowing easier access to the macular region for membrane peeling in highly myopic eyes.

DOI： 10.1097/IAE.0000000000003736

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: To investigate the time courses of morphologic changes in the transplanted grafts, including the retinal layer, the ellipsoid zone (EZ) and the visual acuity (VA) after autologous retinal transplantation (ART) in patients with primary large macular holes (MHs). DESIGN: Single-center, retrospective cohort study. SUBJECTS: The study included 17 eyes of 17 patients who had undergone ART. All patients fulfilled the following criteria: (1) MH was the only disease-causing anatomic abnormality of the macula; (2) they could be followed up for at least 12 months after ART surgery; (3) they had no other systemic disorders; and (4) the MH was > 400 μm in diameter. METHODS: Data of all patients who underwent assessment of the VA and spectral-domain (SD)-OCT at 1, 3, 6, and 12 months after surgery were analyzed. The morphologic features of the graft and the EZ were measured by SD-OCT. MAIN OUTCOME MEASURES: Changes in the morphologic features of the graft (graft thickness, overall graft area, graft length, area of outer retina, and EZ) and VA over 1 year after surgery. RESULTS: Closure of the MH was achieved in all patients. The VA was 0.59 ± 0.27 (logarithm of the minimal angle of resolution units) at 1-year postsurgery compared with 0.84 ± 0.22 before surgery (P < 0.05). The retinal layers gradually became smoothly connected. Although a decrease in graft thickness and overall graft area was observed, the length and area of the outer retina were maintained throughout the follow-up period. The presence of the EZ was observed in 9 patients (52.9%) and our analyses revealed significantly greater improvement of VA in these patients than in those without the EZ (P < 0.05). Moreover, the incidence of macular edema (ME) was higher in the group without the EZ (P = 0.04; chi-square test). CONCLUSION: Patients showed significant improvements of the VA. Morphologically, the layers were gradually connected to each other, and the outer retina was particularly maintained. Especially, patients in whom an EZ was restored in the graft showed a low prevalence of ME and greater improvement of the VA. FINANCIAL DISCLOSURE(S): The author(s) have no proprietary or commercial interest in any materials discussed in this article.

DOI： 10.1016/j.oret.2022.12.004

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Anastomosis on graft retina was studied after autologous retinal transplantation (ART) on 26 eyes with primary and refractory macular holes. Angiogenesis and anastomosis were seen in 35% of the eyes with successful ART, likely contributing to the survival of the transplanted retina.

DOI： 10.1016/j.oret.2022.05.007

PubMed

researchmap

記述言語：英語  

PURPOSE: To evaluate an outbreak of endophthalmitis caused by Fusarium oxysporum after cataract surgery. METHODS: In the present study, we conducted a retrospective review of the medical records of cases of endophthalmitis that developed after cataract surgery. All eyes underwent phacoemulsification and intraocular lens implantation (PEA + IOL) at a single eye clinic on the same date. Symptoms of endophthalmitis occurred 21.5 ± 3.4 days after the cataract surgery. RESULTS: Nine eyes of 9 patients with fungal endophthalmitis (5 males and 4 females) were enrolled in the current study. The mean age of the patients was 63.4 ± 8.5 years. Soon after the diagnosis of endophthalmitis, pars plana vitrectomy (PPV) had been performed in all the eyes. However, because there was no response to the first PPV plus antibacterial drug therapy, we performed repeat PPV for all the eyes, combined with IOL removal and antifungal therapy (natamycin eye drops plus oral voriconazole or fosfluconazole). After the antifungal drug therapy, no recurrence of endophthalmitis was observed in any of the operated eyes, and good visual outcomes were obtained. Fusarium oxysporum was identified by culture and sequencing analysis. CONCLUSION: Early diagnosis and appropriate, adequate treatment are needed for successful management of fungal endophthalmitis.

DOI： 10.1016/j.ajoc.2022.101397

PubMed

researchmap

記述言語：英語  

PURPOSE: To describe the clinical features of severe recurrent fibrovascular proliferation after intravitreal bevacizumab injections and laser photocoagulation for aggressive posterior retinopathy of prematurity. METHODS: This retrospective, nonrandomized case series reviewed the medical and ophthalmic records in the referral hospital and our hospital. PATIENTS: Four patients (seven eyes) with aggressive posterior retinopathy of prematurity. RESULTS: The patients were referred for vitrectomy with/without lensectomy for recurrent fibrovascular proliferation with a tractional retinal detachment after combined intravitreal bevacizumab injections and laser photocoagulation. Three patients were born at 22 weeks or 23 weeks' gestational age and one patient at 29 weeks' gestational age. Preoperatively, fluorescein angiography images showed all eyes had tractional retinal detachment from regrowth of fibrovascular proliferation 3 months to 5 months after the intravitreal bevacizumab injection and abnormal retinal vasculature; four eyes had a broad ischemic retina. Postoperatively, four eyes had retinal attachment and three eyes a total retinal detachment. Neovascular glaucoma developed in five of the seven eyes during the clinical course. CONCLUSION: Severe fibrovascular proliferation may recur due to widespread retinal ischemia with capillary dropout and abnormal vasculature after failed combined intravitreal bevacizumab and laser photocoagulation therapy as the initial treatment for aggressive posterior retinopathy of prematurity. Careful follow-up is important especially after anti-vascular endothelial growth factor treatment, with recognition that severe reactivation is possible.

DOI： 10.1097/ICB.0000000000000887

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: This study investigated the relationship between retinal artery angle and visual function in eyes with idiopathic epiretinal membrane (ERM). METHODS: Ultra-wide field fundus imaging was conducted to analyze ERM and normal contralateral eyes. In addition to the logMAR visual acuity (VA) measurement, the average of the vertical and horizontal metamorphopsia scores (Mave) was measured using m-Charts for each eye. We calculated the retinal artery angle (Yugami correlated angle [YCA]) in all the examined eyes using ImageJ software. The YCAs were then compared between the ERM and normal contralateral eyes. Additionally, the relationship between YCA and visual function was investigated in the ERM eyes. RESULTS: Data from 40 patients with ERM were analyzed. The mean age of the participants was 67.1 ± 8.1 years. The YCA was significantly smaller in the ERM eyes, compared with the contralateral eyes (P < 0.001, respectively; Wilcoxon signed rank test). Among age, axial length, YCAs, central retinal thickness (CRT), and central choroidal thickness (CCT), the optimal model for logMAR VA included age, YCA, and CRT. On the other hand, the optimal model for Mave included YCA and CCT. CONCLUSIONS: The retinal artery angle may be useful for predicting visual function in eyes with ERM. TRANSLATIONAL RELEVANCE: Our established quantitative measurements in fundus photography have potential clinical use in predicting visual function in ERM.

DOI： 10.1167/tvst.10.9.35

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: To describe the vitreoretinal structure at the margin of the choroidal coloboma in infants and older patients using swept-source (SS) OCT. DESIGN: Retrospective case series. PARTICIPANTS: Nineteen eyes of 16 patients with choroidal coloboma (7 males, 9 females; average age, 12.3 ± 7.1 years). METHODS: The patients were classified into 2 groups: infants 1 year of age or younger (3 eyes) and older patients (16 eyes). Each finding on SS OCT was documented according to previously defined histopathologic findings. MAIN OUTCOME MEASURES: Description of the SS OCT features of choroidal colobomas. RESULTS: Swept-source OCT showed that the extracolobomatous retina centrally traversed the margin to continue as the marginal intercalary membrane (MICM), whereas the outer layers of the MICM were reversed at the point (point of reversal [POR]). The expected duplication was seen in all infant eyes, but in none of the older eyes whose outer layers of the MICM were ambiguous. However, at the boundary between the layered MICM and monolayered central intercalary membrane (CICM), the POR was detectable in all patients. Further SS OCT analysis showed that the MICM schisis and CICM schisis occurred simultaneously with vitreous traction. Retinal detachments (RDs) seen in 4 eyes were connected to the only MICM schisis, and a MICM break was identified in 1 eye. Swept-source OCT showed that retinal pigment epithelial hyperplasia adhered tightly to the retina and that the glial triangle was adhered tightly to the sclera, indicating barriers to the development of RD after MICM schisis. CONCLUSIONS: Swept-source OCT first visualized the POR in infant eyes and showed that the POR was identifiable despite the atrophic changes in the outer layer of the MICM in the older eyes. Based on the POR location, we confirmed that the intercalary membranes reported in previous OCT studies were clearly differentiated between the MICM and CICM. We also showed that the presence of MICM and CICM schisis resulted from vitreous traction at the coloboma margin and that MICM breaks induced RD only if the barrier that prevented the development of RD was broken.

DOI： 10.1016/j.oret.2020.10.012

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: To investigate the associations between visual function and the optical coherence tomography (OCT) parameters in eyes with idiopathic epiretinal membrane (ERM). METHODS: Thirty-nine consecutive eyes with ERM were enrolled. In addition to OCT parameters, such as central retinal thickness (CRT), the area of gap between the ERM and the retinal surface (SUKIMA) was newly defined and calculated from the vertical and horizontal OCT images (SUKIMAv and SUKIMAh). The average of SUKIMAv and SUKIMAh (SUKIMAave) was used for the statistical analysis. The vertical and horizontal metamorphopsia scores (MV, MH) and the average of MV and MH (Mave) were also used for the analysis. RESULTS: The Mave was not significantly associated with logMAR visual acuity (VA) (P = 0.57, linear regression analysis). Analysis using second-order bias-corrected Akaike information criterion model selection identified the age, CRT, and SUKIMAave as being associated with logMAR VA. On the other hand, among the OCT parameters, SUKIMAave and CRT were associated with the Mave. In addition, there was a significant relationship between SUKIMAh and MV (P = 0.011) and between SUKIMAv and MH (P = 0.0014). CONCLUSIONS: We identified SUKIMA as a novel OCT parameter that is useful to predict both VA and metamorphopsia in patients with ERM.

DOI： 10.1167/iovs.62.6.6

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: To examine the usefulness of red channel fundus imaging to detect the ischemic status in eyes with central retinal vein occlusion (CRVO). METHODS: Ultra-widefield (UWF) fundus images were obtained from 42 eyes with CRVO. Twenty-one eyes were ischemic, and 21 eyes were non-ischemic. Rubeosis was found in 11 ischemic eyes. UWF images were split into red and green channels using ImageJ software. Both the color and red channel images were used to predict the presence or absence of ischemia when examined by masked graders. The sensitivity and specificity of UWF imagings for the detection of ischemia were calculated in Group A (total 42 eyes), Group B (32 eyes excluding non-rubeotic ischemic CRVO) and Group C (31 eyes excluding rubeotic ischemic CRVO), respectively. Moreover, a linear mixed model was conducted to investigate the relationship between the type of images and the accuracy of prediction in each group. RESULTS: No significant difference in the sensitivity of color fundus imaging was seen between Group A and Group B. By contrast, a significant difference in the sensitivity of red channel imaging was seen between Group A and Group B (p = 0.031). The accuracies of the predictions were not associated with the type of image in Group A and Group B, but were significantly associated in Group C (p = 0.026). CONCLUSIONS: UWF red channel imaging enabled more accurate detection of the ischemic status, compared with color fundus images, especially in non-rubeotic CRVO eyes.

DOI： 10.1371/journal.pone.0260383

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: To describe the clinical features and treatment outcomes of severe retinopathy in eyes with incontinentia pigmenti (IP) of infants within a few months of birth. STUDY DESIGN: Retrospective clinical study. METHODS: Six eyes of three patients (6-day-old girl, 5-month-old girl, and 14-day-old boy) with IP were examined and treated under general anesthesia. Ophthalmologic examinations were performed including images from wide-angle fluorescein angiography (FA), swept-source optical coherence tomography (OCT), and OCT angiography (OCTA). RESULTS: Ophthalmoscopy showed prominent vascular tortuosity in five eyes, retinal hemorrhages in four eyes, and incomplete vascular development in two eyes. FA showed extensive avascularity including the posterior pole of the retina in all cases except one eye. Prompt and intensive laser photocoagulation stabilized the pre-proliferative severe retinopathy in five eyes; however, foveal structure and vessel anomalies were detected in three of six eyes by OCT and two of five eyes by OCTA. CONCLUSION: Severe retinopathy in the neonatal period and infancy was present not only in the periphery but also in the posterior pole including the fovea, which might be related to retinal vascular maldevelopment. It is, therefore, recommended that wide-angle fundus FA examination be performed in the early postnatal period to detect early signs of severe retinopathy in infants with IP.

DOI： 10.1007/s10384-020-00768-7

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: To report the outcomes of autologous neurosensory retinal transplant as a primary treatment for patients with large chronic macular holes and evaluate the safety and feasibility of the procedure. DESIGN: Retrospective study, consecutive case series. METHODS: We reviewed seven patients with a primary chronic large macular hole, who underwent autologous neurosensory retinal transplant. Mean preoperative minimum and maximum hole diameters were 643 µm and 1214 µm, respectively. Changes in visual acuity were measured postsurgery, and optical coherence tomography, fluorescein angiography, and microperimetry-3 were analyzed after the procedure. RESULTS: Closure of the macular hole was achieved in all seven eyes in the study. At 1 year post-surgery, there was significant improvement in mean visual acuity (LogMAR 1.10 vs. 0.68, P = 0.001). Optical coherence tomography showed that all grafts had formed attachments to the retinal epithelial cells of the recipient retina. Mean preoperative ellipsoid zone defect was 1,089 ± 403.8 µm (range, 918-1,329 µm) which further decreased to 921 ± 129.1 µm (range, 670-1,201 µm) at final follow up (P = 0.09). Microperimetry-3 testing indicated retinal sensitivity in the graft in five eyes. CONCLUSION: Autologous retinal transplantation may help rebuild the macular structure resulting in functional improvement for eyes with primary chronic large macular hole.

DOI： 10.1097/IAE.0000000000002693

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1001/jamaophthalmol.2018.4197

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE: Sarcoidosis is a major cause of granulomatous uveitis but rarely manifests as multiple choroidal granulomas. This report describes the use of enhanced depth imaging optical coherence tomography (EDI-OCT) to visualize changes occurring in multiple choroidal granulomas during treatment. METHODS: The patient was a 36-year-old Japanese man with histopathologically confirmed sarcoidosis, who was examined using EDI-OCT and showed multiple yellowish-white subretinal lesions in the peripapillary region and the arcade of the right eye. RESULTS: EDI-OCT revealed homogeneous hyporeflective choroidal lesions with choriocapillaris thinning, consistent with a diagnosis of choroidal granulomas. Subretinal fluid adjacent to one of the peripapillary choroidal lesions was also apparent. EDI-OCT during oral prednisolone administration revealed a decrease in lesion size at as early as 3 weeks and complete resolution of the lesions after 6 months of treatment. However, 2 months after prednisolone discontinuation, EDI-OCT revealed recurrence of choroidal granulomas in the peripapillary region and the arcade of the right eye. After injection of triamcinolone acetonide into the posterior sub-Tenon's capsule (sub-Tenon's injection), EDI-OCT demonstrated a reduction in granuloma lesion size within 3 months of the injection. CONCLUSION: EDI-OCT allowed detailed morphologic visualization of the choroidal granulomas caused by sarcoidosis. This imaging technique was useful for monitoring changes in granuloma size in response to steroid administration and for early detection of recurrence. Injection of triamcinolone acetonide into the posterior sub-Tenon's capsule was as effective as oral prednisolone for the treatment of choroidal granulomas.

DOI： 10.1007/s10792-017-0720-2

PubMed

researchmap

記述言語：英語  

PURPOSE: To report the clinical and genetic findings of a male toddler who presented bilateral bullous retinoschisis with a novel RS1 mutation. OBSERVATIONS: This is an observational case report of a patient referred to our hospital with esotropia. A comprehensive ophthalmic examination was performed with the boy (age, 1 year 4 months) under general anesthesia that included fundus examinations, fluorescein angiography (FA), swept-source optical coherence tomography (SS-OCT), and full-field electroretinography (FF-ERG). Genetic analysis of the coding region in the RS1 gene was performed by Sanger sequencing for the patient and mother. There was a family history of X-linked retinoschisis (XLRS). Fundus examinations and FA showed bullous retinoschisis bilaterally in the inferior retina. The SS-OCT images showed two kinds of schisis in the inner nuclear layer (INL) and more proximally. In general, the inner plexiform layer, ganglion cell layer, and retinal nerve fiber layer are in the proximal INL; however, in this case there was hyperreflective tissue with a rough surface instead of normal retinal layers. In addition, in the schisis cavity between the hyperreflective tissue and separated retina, a number of hyperreflective fiber-like strands arose from the hyperreflective tissue and extended to the schisis cavity. During the follow-up period, the bullous retinoschisis collapsed spontaneously in the right eye. FF-ERG showed a reduced b-wave and relatively preserved a-wave in all components. Genetic analysis showed a novel RS1 mutation (c.185_186insT, p.E62DfsX24 in exon 4) in the patient and mother. CONCLUSIONS AND IMPORTANCE: We report the detailed retinal structure in a genetically identified case of bullous retinoschisis. The notable finding was that the cavity of bullous retinoschisis contained a number of fiber-like strands as observed in the cavity of typical retinoschisis.

DOI： 10.1016/j.ajoc.2016.12.009

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Islet transplantation can induce a substantial improvement in the treatment of type 1 diabetes mellitus. However, the clinical application of islet transplantation is severely limited by the shortage of donor organs. It is thus essential to improve the engraftment rate to achieve the expected outcome in the treatment of diabetes mellitus using a limited amount of donor islets. In this manuscript, we describe the generation of β-cell spheroids using mouse insulinoma cells (MIN6) as a model of β-cells. We established a 3D culture system that simulates microgravity using a 3D clinostat. Using this method, we were able to produce 100 spheroids per mL of culture media. The optimization of the culture conditions in the clinostat produced spheroids with a size of approximately 250 μm, which is a size that is known to induce good graft survival after islet transplantation. The spheroids produced in the clinostat expressed several β-cell signature genes at higher levels than the levels that were found in MIN6 cells that were cultured in a standard 2D culture dish (MIN6-2D). The transplantation of the spheroids into the portal vein of streptozotocin-induced diabetic mice ameliorates hyperglycemia, whereas the transplantation of the equivalent number of 2D-cultured cells failed to cure diabetes. These results indicate that the clinostat culture provides a new method for the reconstitution of a large number of functional β-cell spheroids for diabetes treatment.

DOI： 10.1016/j.biomaterials.2013.04.003

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND: Careful orchestration among endodermal epithelial, endothelial, and mesenchymal cells initiate liver organogenesis prior to vascular function. Nonparenchymal endothelial or mesenchymal cells not only form passive conduits, but also establish an organogenic stimulus. Herein, we have evaluated the potential roles of primitive endothelial and mesenchymal cells toward hepatic organization in vitro. METHODS: To track the cellular movements and localization, we retrovirally transduced enhanced green fluorescence protein and kusabira orange into human fetal liver cells (GFP-hFLCs) and human umbilical vein endothelial cells (KO-HUVECs), respectively. GFP-hFLCs were cocultivated with KO-HUVECs and human mesenchymal stem cells (hMSCs) under conventional two-dimensional (2D) conditions. RESULTS: Even under 2D culture, fetal liver, endothelial, and mesenchymal cells self-organized into a macroscopically visible three-dimensional (3D) organoid. Time-lapse confocal imaging showed dynamic cellular organizations of GFP-hFLCs and KO-HUVECs. Endothelial cells organized into patterned clusters wrapping fetal liver cells, forming vessel-like lumens inside. Mesenchymal cells supported the generated organoid from outside. CONCLUSION: Generation of whole organ architecture remains a great challenge so far. Our preliminary results showed that recapitulation of primitive cellular interactions during organogenesis elicit the intrinsic self-organizing capacity to form hepatic organoids. Future studies to define precise conditions mimicking organogenesis may ultimately lead to the generation of a functional liver for transplantation and for other applications such as drug development.

DOI： 10.1016/j.transproceed.2012.02.007

PubMed

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：(株)先端医学社  

近年,外科領域では手術支援ロボットの普及が進んでいる.われわれは,術者の腕の安定化を目的にパッシブ型手術支援用ロボットiArmSを,眼科手術を念頭にカスタマイズした.パッシブ型ロボットはモーター駆動でないことから誤作動の可能性がなく,術者の操作を受動的に補完することが可能となる.加速度センサを用いた計測により,パッシブ型ロボットの使用は網膜血管内注入時の手ぶれを抑制することが示唆された.(著者抄録)

researchmap

記述言語：日本語   出版者・発行元：(株)メディカ出版  

researchmap

その他リンク： https://search.jamas.or.jp/default/link?pub_year=2024&ichushi_jid=J06107&link_issn=&doc_id=20240523230006&doc_link_id=issn%3D2187-2422%26volume%3D13%26issue%3D3%26spage%3D294&url=http%3A%2F%2Fwww.pieronline.jp%2Fopenurl%3Fissn%3D2187-2422%26volume%3D13%26issue%3D3%26spage%3D294&type=PierOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00005_2.gif

記述言語：日本語   出版者・発行元：(公社)日本眼科手術学会  

小児の黄斑上膜(ERM)はまれな疾患で,外傷やぶどう膜炎やその他の網膜疾患に続発して起こることが多い.成人のERMと比較して網膜や血管への付着が強く,症状の進行が急速に起こることもある.一方,自然剥離することも多く,比較的長期の経過観察が重要である.視力低下や歪視の進行がみられる場合には硝子体手術が検討される.硝子体手術では後部硝子体剥離が起きていない点や,ERMが網膜に強く接着していることに注意する.また,内境界膜(ILM)も除去することで再発の防止になる.多くの症例では術後に視力の改善が得られる.(著者抄録)

researchmap

記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

researchmap

記述言語：日本語   出版者・発行元：金原出版(株)  

＜文献概要＞巨大な黄斑円孔もしくは難治性黄斑円孔により,網膜自家移植術を受け5年以上の経過を観察することができた3例3眼を報告する。移植術を受けた際の平均年齢,平均術後観察期間,平均最小黄斑円孔径,平均視力はそれぞれ,62.3歳(男性3),67±6ヵ月,902±176μm,0.12±0.09であった。一方,最終受診時の移植網膜厚,移植網膜内のEZ長,術後視力は,それぞれ209±51μm,112±8μm,0.40±0.4であった。経過観察中に,移植片の離脱,強度の術後炎症をきたした症例はみられなかった。網膜自家移植術の術後経過は長期に安定しており,難治性の黄斑円孔に対して有効な手術治療と考えられる。

researchmap

記述言語：日本語   出版者・発行元：金原出版(株)  

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：日本糖尿病眼学会  

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：(株)メディカ出版  

researchmap

その他リンク： https://search.jamas.or.jp/default/link?pub_year=2021&ichushi_jid=J06107&link_issn=&doc_id=20211119170003&doc_link_id=issn%3D2187-2422%26volume%3D10%26issue%3D6%26spage%3D637&url=http%3A%2F%2Fwww.pieronline.jp%2Fopenurl%3Fissn%3D2187-2422%26volume%3D10%26issue%3D6%26spage%3D637&type=PierOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00005_2.gif

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：金原出版(株)  

researchmap

記述言語：日本語   出版者・発行元：(一社)日本糖尿病合併症学会  

researchmap

記述言語：日本語   出版者・発行元：金原出版(株)  

＜文献概要＞網膜中心動脈閉塞症(CRAO)は血栓や塞栓により網膜中心動脈が閉塞する疾患である。高齢者に多く,無痛性かつ急激に単眼性に発症する。その危険因子は脳梗塞や心筋梗塞といった他の虚血性疾患と同様である。CRAOは4つに分類され,特に動脈炎性CRAOでは治療方針が異なるので注意が必要である。非動脈炎性CRAOの急性期治療に関して有効性の確立されたものはないが,発症から1～2日では再灌流を目指し施設によってできうる限りの治療を行う。慢性期には血管新生緑内障や他眼・他臓器の虚血性疾患を予防するための診察や検査を行う。

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：(株)医学書院  

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：金原出版(株)  

＜文献概要＞目的 網膜中心動脈閉塞症(CRAO)の網膜内循環時間の測定におけるレーザースペックルフローグラフィ(LSFG)の有用性を検討する。対象および方法 横浜市立大学附属市民総合医療センター眼科において2015年5月から2019年2月までにCRAOと診断された患者28例28眼(男性22例22眼,女性6例6眼,平均年齢74.0±10.6歳)を対象とした。フルオレセイン蛍光眼底造影検査(FA)を用いて網膜内循環時間(RC time)を測定した。また,LSFGを用いてCRAOと診断された患者の視神経乳頭部の血流値(MBR)を乳頭部血管成分(MV),乳頭部組織成分(MT)の各血流値に分類し,MV-MTを測定した。また,MV-MT,RC time,最高矯正視力(logMAR)との関連性も検討した。統計にはSpearmanの順位相関行列を用い,P<0.05を統計学的有意とした。結果 MV-MT,RC timeおよびlogMARの平均値は,それぞれ7.7±5.9,194.8±231.7秒,2.0±0.8であった。MV-MTとRC timeとの間に有意な相関があった(r=-0.65,P<0.001)。また,LogMARとMV-MT(r=-0.49,P=0.03),LogMARとRC time(r=0.43,P=0.02)の間にも有意な相関がみられた。結論 CRAO眼のLSFGの測定は,安全かつ簡便に行うことができる。LSFGはCRAO眼の循環時間の評価の有用な手段となり得る。

researchmap

その他リンク： https://search.jamas.or.jp/default/link?pub_year=2020&ichushi_jid=J00293&link_issn=&doc_id=20200609270014&doc_link_id=10.18888%2Fga.0000001662&url=https%3A%2F%2Fdoi.org%2F10.18888%2Fga.0000001662&type=%E5%8C%BB%E6%9B%B8.jp_%E3%82%AA%E3%83%BC%E3%83%AB%E3%82%A2%E3%82%AF%E3%82%BB%E3%82%B9&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00024_2.gif

J-GLOBAL

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

背景:斜位近視は小児では稀な病態である。斜位近視を契機に小脳腫瘍が発見された小児例を経験したため報告する。症例:11歳女児。5歳時には外斜位8Δ、立体視50秒であった。1年前より外斜視が悪化し、両目で見るとぼやけると訴え来院した。視力は右眼1.2、左眼0.2(1.2)、眼位は正位から外斜視50Δへ変動、眼球運動制限はないが輻湊は不良で、両眼視機能は低下していた。非調節麻痺下屈折検査にて著明な近視の変動があり、両眼開放下屈折検査では外斜視時に比べ正位努力時に強い近視化と縮瞳を認め、斜位近視と診断した。前眼部および眼底に異常はなかった。頭部MRIを施行したところ右小脳半球内側から第四脳室に突出する小脳腫瘍を認めた。腫瘍は摘出され、毛様細胞性星細胞腫と病理診断された。結論:本症例は小脳腫瘍によって輻湊力が低下し、斜位近視を生じたと考えられる。小児における斜位近視は頭蓋内病変を考慮する必要がある。(著者抄録)

researchmap

記述言語：日本語   出版者・発行元：(株)メディカ出版  

researchmap

その他リンク： https://search.jamas.or.jp/default/link?pub_year=2019&ichushi_jid=J06107&link_issn=&doc_id=20190314160009&doc_link_id=%2Fao7gagre%2F2019%2F000802%2F012%2F0195-0201%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fao7gagre%2F2019%2F000802%2F012%2F0195-0201%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

目的:光干渉断層計(OCT)を用いて網膜静脈分枝閉塞症(BRVO)の動静脈交叉部を観察し、交叉部形態の特徴を調べる。対象と方法:動静脈交叉部を同定できたBRVOの連続症例111例111眼を対象とし、OCTを用いて動静脈交叉部を中心に2方向撮影を行い、交叉部の動静脈交叉パターン、交叉部静脈の狭窄の有無、交叉部静脈内血栓の有無と存在部位を観察した。結果:閉塞部の動静脈交叉パターンは動脈が静脈の上を走行するAVパターンが55眼(49.5%)、静脈が動脈の上を走行するVAパターンが56眼(50.5%)であった。交叉部静脈の狭窄は9眼(8.1%)でみられ、すべてVAパターンで、内境界膜と交叉部動脈に圧迫されていた。102眼(91.9%)では交叉部静脈の彎曲はみられるものの内腔は保たれていた。交叉部静脈内血栓は15眼(13.5%)でみられ、内訳はAVパターン55眼中2眼(3.6%)とVAパターン56眼中13眼(23.2%)であった。血栓の部位は、交叉部の上流が3眼、交叉部が4眼、下流が5眼、交叉部から下流にまたがるものが1眼、交叉部の上流から下流にまたがるものが2眼であった。結論:従来AVパターンが多いといわれていたが、約半数の動静脈交叉部はVAパターンであった。OCTはBRVOにおける動静脈交叉部形態の観察に有用であった。(著者抄録)

researchmap

その他リンク： https://search.jamas.or.jp/default/link?pub_year=2018&ichushi_jid=J01049&link_issn=&doc_id=20181213310003&doc_link_id=%2Fdz1nigan%2F2018%2F012212%2F003%2F0920-0927%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdz1nigan%2F2018%2F012212%2F003%2F0920-0927%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

記述言語：日本語   出版者・発行元：眼科臨床紀要会  

researchmap

記述言語：日本語   出版者・発行元：(株)全日本病院出版会  

網膜動脈閉塞症(RAO)は血栓や塞栓により網膜動脈が閉塞する疾患である。高齢者に多く、無痛性かつ急激に単眼性に発症する。RAOのリスクファクターは脳梗塞や心筋梗塞といった他の虚血性疾患と同様である。したがって、再灌流を試みる急性期治療を行いながら血管閉塞の原因を発見し、慢性期には新生血管緑内障や他臓器の虚血イベントを予防することが重要である。急性期の治療に関しては有効性の確立されたものはないが、発症から24時間以内の患者には施設によってできうる限りの治療を行う。近年注目されている血栓溶解療法に対しては2つの無作為化臨床試験が行われたが、有効性は証明されていない。しかし、発症早期であれば有効である可能性も示唆されており、今後の治療の発展に注目する必要がある。(著者抄録)

researchmap