DOI： 10.1007/s10532-023-10014-9

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担当区分：最終著者,　責任著者   掲載種別：研究論文（学術雑誌）  

添付ファイル： spectrum.03149-22-1.pdf

DOI： 10.1128/spectrum.03149-22

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掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ibiod.2022.105500

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担当区分：最終著者,　責任著者   掲載種別：研究論文（学術雑誌）  

添付ファイル： Kayama MRA 2022.pdf

DOI： 10.1128/mra.00532-22

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添付ファイル： microorganisms-10-00510.pdf

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担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）  

添付ファイル： Mori & Kanaly Microbiol Spectr 2022.pdf

DOI： 10.1128/spectrum.02225-21

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掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

添付ファイル： 1-s2.0-S2772416621000188-main.pdf

DOI： 10.1016/j.hazadv.2021.100018

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担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）  

添付ファイル： Mori MRA KK10.pdf

DOI： 10.1128/mra.00423-21

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掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.petrol.2021.108870

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担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）  

添付ファイル： Mori & Kanaly 2021 MRA.pdf

DOI： 10.1128/mra.01250-20

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担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）  

DOI： 10.1128/aem.02268-20

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掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10230-020-00711-9

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その他リンク： http://link.springer.com/article/10.1007/s10230-020-00711-9/fulltext.html

掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ibiod.2020.104993

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添付ファイル： fmicb-10-02435(2).pdf

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添付ファイル： mSystems-2019-Chen-e00410-19.full(1).pdf

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添付ファイル： fmicb-10-00297(1).pdf

DOI： 10.3389/fmicb.2019.00297

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：NATURE PUBLISHING GROUP  

Chemosynthetic Fe-oxidizing communities are common at diffuse-flow hydrothermal vents throughout the world's oceans. The foundational members of these communities are the Zetaproteobacteria, a class of Proteobacteria that is primarily associated with ecosystems fueled by ferrous iron, Fe(II). We report here the discovery of two new isolates of Zetaproteobacteria isolated from the Mid-Atlantic Ridge (TAG-1), and the Mariana back-arc (SV-108), that are unique in that they can utilize either Fe(II) or molecular hydrogen (H-2) as sole electron donor and oxygen as terminal electron acceptor for growth. Both strains precipitated Fe-oxyhydroxides as amorphous particulates. The cell doubling time on H-2 vs Fe(II) for TAG-1 was 14.1 vs 21.8 h, and for SV-108 it was 16.3 vs 20 h, and it appeared both strains could use either H-2 or Fe(II) simultaneously. The strains were close relatives, based on genomic analysis, and both possessed genes for the uptake NiFe-hydrogenase required for growth on H-2. These two strains belong to Zetaproteobacteria operational taxonomic unit 9 (ZetaOTU9). A meta-analysis of public databases found ZetaOTU9 was only associated with Fe(II)-rich habitats, and not in other environments where known H-2-oxidizers exist. These results expand the metabolic repertoire of the Zetaproteobacteria, yet confirm that Fe(II) metabolism is the primary driver of their physiology and ecology.

添付ファイル： ismej2017132a(1).pdf

DOI： 10.1038/ismej.2017.132

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

添付ファイル： ismej2016186a.pdf

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MICROBIOLOGY SOC  

A new acidophilic iron-oxidizing strain (C25) belonging to the novel genus Acidithrix was isolated from pelagic iron-rich aggregates ('iron snow') collected below the redoxcline of an acidic lignite mine lake. Strain C25 catalysed the oxidation of ferrous iron [Fe(II)] under oxic conditions at 25 degrees C at a rate of 3.8 mM Fe(II) day(-1) in synthetic medium and 3.0 mM Fe(II) day(-1) in sterilized lake water in the presence of yeast extract, producing the rust-coloured, poorly crystalline mineral schwertmannite [Fe(III) oxyhydroxylsulfate]. During growth, rod-shaped cells of strain C25 formed long filaments, and then aggregated and degraded into shorter fragments, building large cell mineral aggregates in the late stationary phase. Scanning electron microscopy analysis of cells during the early growth phase revealed that Fe(III)-minerals were formed as single needles on the cell surface, whereas the typical pincushion-like schwertmannite was observed during later growth phases at junctions between the cells, leaving major parts of the cell not encrusted. This directed mechanism of biomineralization at specific locations on the cell surface has not been reported from other acidophilic iron-oxidizing bacteria. Strain C25 was also capable of reducing Fe(III) under micro-oxic conditions which led to a dissolution of the Fe(III)-minerals. Thus, strain C25 appeared to have ecological relevance for both the formation and transformation of the pelagic iron-rich aggregates at oxic/anoxic transition zones in the acidic lignite mine lake.

DOI： 10.1099/mic.0.000205

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：COPERNICUS GESELLSCHAFT MBH  

Filamentous macroscopic algae were observed in slightly acidic to circumneutral (pH 5.9-6.5), metal-rich stream water that leaked out from a former uranium mining district (Ronneburg, Germany). These algae differed in color and morphology and were encrusted with Fedeposits. To elucidate their potential interaction with Fe(II)-oxidizing bacteria (FeOB), we collected algal samples at three time points during summer 2013 and studied the algae-bacteria-mineral compositions via confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectra, and a 16S and 18S rRNA gene-based bacterial and algae community analysis. Surprisingly, sequencing analysis of 18S rRNA gene regions of green and brown algae revealed high homologies with the freshwater algae Tribonema (99.9-100 %). CLSM imaging indicated a loss of active chloroplasts in the algae cells, which may be responsible for the change in color in Tribonema. Fe(III)-precipitates on algal cells identified as ferrihydrite and schwertmannite by FTIR were associated with microbes and extracellular polymeric substances (EPS)-like glycoconjugates. SEM imaging revealed that while the green algae were fully encrusted with Fe-precipitates, the brown algae often exhibited discontinuous series of precipitates. This pattern was likely due to the intercalary growth of algal filaments which allowed them to avoid detrimental encrustation. 16S rRNA gene-targeted studies revealed that Gallionella-related FeOB dominated the bacterial RNA and DNA communities (70-97 and 63-96 %, respectively), suggesting their capacity to compete with the abiotic Feoxidation under the putative oxygen-saturated conditions that occur in association with photosynthetic algae. Quantitative PCR (polymerase chain reaction) revealed even higher Gallionella-related 16S rRNA gene copy numbers on the surface of green algae compared to the brown algae. The latter harbored a higher microbial diversity, including some putative predators of algae. A loss of chloroplasts in the brown algae could have led to lower photosynthetic activities and reduced EPS production, which is known to affect predator colonization. Collectively, our results suggest the coexistence of oxygen-generating algae Tribonema sp. and strictly microaerophilic neutrophilic FeOB in a heavy metal-rich environment.

添付ファイル： bg-12-5277-2015.pdf

DOI： 10.5194/bg-12-5277-2015

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会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：ポスター発表  

添付ファイル： ISME 2018 poster JM_final.pdf

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会議種別：口頭発表（一般）  

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会議種別：ポスター発表  

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会議種別：ポスター発表  

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会議種別：ポスター発表  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：ポスター発表  

添付ファイル： Mori ISME16 Final.pdf

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記述言語：英語   会議種別：ポスター発表  

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会議種別：ポスター発表  

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会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：ポスター発表  

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会議種別：口頭発表（一般）  

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会議種別：ポスター発表  

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