Updated on 2026/03/11

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写真a

 
Kanako Kawaura
 
Organization
Graduate School of Nanobioscience Department of Life and Environmental System Science Associate Professor
School of Science Department of Science
Title
Associate Professor
External link

Degree

  • 博士（農学） ( 京都大学 )

Research Interests

  • コムギ

  • ゲノム

  • 種子貯蔵タンパク質

  • 遺伝子発現

  • アレルギー

  • 遺伝子

  • 発現遺伝子

  • グルテン

Research Areas

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

Education

  • 京都大学大学院農学研究科

    1997.4 - 1999.3

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  • Kyoto University   Faculty of Agriculture

    - 1997

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    Country： Japan

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Research History

  • Yokohama City University   Associate Professor

    2015

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  • Yokohama City University International College of Arts and Sciences Life and Environmental Science Graduate School of Nanobioscience Department of Life and Environmental System Science   Associate Professor

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Professional Memberships

Papers

  • Purifying selection on deleterious variants affected by the combination of subgenomes and gene expression in bread wheat

    Gwyneth Halstead-Nussloch, Moeko Okada, Georg Haberer, Thomas Lux, A.S.M. Faridul Islam, Masaomi Hatakeyama, Roman Briskine, Benjamen White, Anthony Hall, Curtis Pozniak, Sean Walkowiak, Valentyna Klymiuk, Brook Byrns, Kirby Nilsen, Jennifer Ens, Krystalee Wiebe, Amidou N’ Diaye, Pierre J. Hucl, Curtis Pozniak, Bin Xiao Fu, Liangliang Gao, Emily Delorean, Dal-Hoe Koo, Allen K. Fritz, Jesse A. Poland, Cecile Monat, Axel Himmelbach, Anne Fiebig, Sudharsan Padmarasu, Uwe Scholz, Martin Mascher, Nils Stein, Georg Haberer, Heidrun Gundlach, Klaus F.X. Mayer, Manuel Spannagl, Mulualem T. Kassa, Pierre Fobert, Sateesh Kagale, Jemima Brinton, Ricardo H. Ramirez-Gonzalez, Michael Bevan, Neil McKenzie, Burkhard Steuernagel, Cristobal Uauy, Markus C. Kolodziej, Simon G. Krattinger, Beat Keller, Thomas Wicker, Dinushika Thambugala, Curt A. McCartney, Venkat Bandi, Jorge Nunez Siri, Carl Gutwin, Catharine Aquino, Masaomi Hatakeyama, Dario Copetti, Gwyneth Halstead-Nussloch, Timothy Paape, Rie Shimizu-Inatsugi, Kentaro K. Shimizu, Tomohiro Ban, Kanako Kawaura, Toshiaki Tameshige, Hiroyuki Tsuji, Luca Venturini, Matthew Clark, BernardoClavijo, Christine Fosker, Gonzalo Garcia Accinelli, Darren Heavens, Ksenia Krasileva, David Swarbreck, Jonathan Wright, Anthony Hall, Keith A. Gardner, Nick Fradgley, Lawrence Percival-Alwyn, James Cockram, Juan Gutierrez-Gonzalez, Gary Muehlbauer, Chu Shin Koh, Andrew G. Sharpe, Jasline Deek, Alejandro C. Costamagna, Hiroyuki Kanamori, Fuminori Kobayashi, Tsuyoshi Tanaka, Jianzhong Wu, Hirokazu Handa, Tony Kuo, Jun Sese, Kazuki Murata, Yusuke Nabeka, Shuhei Nasuda, Philomin Juliana, Ravi Singh, Hikmet Budak, Ian Small, Joanna Melonek, Sylvie Cloutier, Gabriel Keeble-Gagnère, Josquin Tibbets, Erik Legg, Arvind Bharti, Peter Langridge, KenChalmers, Assaf Distelfeld, Manuel Spannagl, Timothy Paape, Kentaro K. Shimizu

    Cell Reports   116785 - 116785   2026.1

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.celrep.2025.116785

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  • De novo annotation reveals transcriptomic complexity across the hexaploid wheat pan-genome

    Benjamen White, Thomas Lux, Rachel Rusholme-Pilcher, Angéla Juhász, Gemy Kaithakottil, Susan Duncan, James Simmonds, Hannah Rees, Jonathan Wright, Joshua Colmer, Sabrina Ward, Ryan Joynson, Benedict Coombes, Naomi Irish, Suzanne Henderson, Tom Barker, Helen Chapman, Leah Catchpole, Karim Gharbi, Utpal Bose, Moeko Okada, Hirokazu Handa, Shuhei Nasuda, Kentaro K. Shimizu, Heidrun Gundlach, Daniel Lang, Guy Naamati, Erik J. Legg, Arvind K. Bharti, Michelle L. Colgrave, Wilfried Haerty, Cristobal Uauy, David Swarbreck, Philippa Borrill, Jesse A. Poland, Simon G. Krattinger, Nils Stein, Klaus F. X. Mayer, Curtis Pozniak, Sean Walkowiak, Valentyna Klymiuk, Brook Byrns, Kirby Nilsen, Jennifer Ens, Krystalee Wiebe, Amidou N’Diaye, Pierre J. Hucl, Curtis J. Pozniak, Bin Xiao Fu, Liangliang Gao, Emily Delorean, Dal-Hoe Koo, Allen K. Fritz, Jesse Poland, Cecile Monat, Axel Himmelbach, Anne Fiebig, Sudharsan Padmarasu, Uwe Scholz, Martin Mascher, Georg Haberer, Mulualem T. Kassa, Pierre Fobert, Sateesh Kagale, Jemima Brinton, Ricardo H. Ramirez-Gonzalez, Michael Bevan, Neil McKenzie, Burkhard Steuernagel, Markus C. Kolodziej, Simon G. Krattinger, Beat Keller, Thomas Wicker, Dinushika Thambugala, Curt A. McCartney, Venkat Bandi, Jorge Nunez Siri, Carl Gutwin, Catharine Aquino, Masaomi Hatakeyama, Dario Copetti, Gwyneth Halstead-Nussloch, Timothy Paape, Rie Shimizu-Inatsugi, Kentaro K. Shimizu, Tomohiro Ban, Kanako Kawaura, Toshiaki Tameshige, Hiroyuki Tsuji, Luca Venturini, Matthew Clark, Bernardo Clavijo, Christine Fosker, Gonzalo Garcia Accinelli, Darren Heavens, Ksenia Krasileva, Keith A. Gardner, Nick Fradgley, Lawrence Percival-Alwyn, James Cockram, Juan Gutierrez-Gonzalez, Gary Muehlbauer, Chu Shin Koh, Andrew G. Sharpe, Jasline Deek, Alejandro C. Costamagna, Hiroyuki Kanamori, Fuminori Kobayashi, Tsuyoshi Tanaka, Jianzhong Wu, Hirokazu Handa, Tony Kuo, Jun Sese, Kazuki Murata, Yusuke Nabeka, Shuhei Nasuda, Philomin Juliana, Ravi Singh, Hikmet Budak, Ian Small, Joanna Melonek, Sylvie Cloutier, Gabriel Keeble-Gagnère, Josquin Tibbets, Erik Legg, Arvind Bharti, Peter Langridge, Ken Chalmers, Assaf Distelfeld, Manuel Spannagl, Anthony Hall

    Nature Communications   16 ( 1 )   2025.10

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    Wheat is the most widely cultivated crop in the world, with over 215 million hectares grown annually. The 10+ Wheat Genomes Project recently sequenced and assembled to chromosome-level the genomes of nine wheat cultivars, uncovering genetic diversity and selection within the pan-genome of wheat. Here, we provide a wheat pan-transcriptome with de novo annotation and differential expression analysis for these wheat cultivars across multiple tissues. Using the de novo annotations we identify cultivar-specific genes and define the core and dispensable genomes. Expression analysis across cultivars and tissues reveals conservation in expression between a large core set of homeologous genes, in addition to widespread changes in subgenome homeolog expression bias between cultivars and cultivar-specific expression profiles. We utilise both the newly constructed gene-based wheat pan-genome and pan-transcriptome, demonstrating variation in the prolamin superfamily and immune-reactive proteins across cultivars.

    DOI： 10.1038/s41467-025-64046-1

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    Other Link： https://www.nature.com/articles/s41467-025-64046-1

  • Decrease in purifying selection pressures on wheat homoeologous genes: tetraploidization versus hexaploidization Reviewed

    Akihiro Ezoe, Daisuke Todaka, Yoshinori Utsumi, Satoshi Takahashi, Kanako Kawaura, Motoaki Seki

    The Plant Journal   120 ( 3 )   1190 - 1205   2024.10

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    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    SUMMARY

    A series of polyploidizations in higher‐order polyploids is the main event affecting gene content in a genome. Each polyploidization event can lead to massive functional divergence because of the subsequent decrease in selection pressure on duplicated genes; however, the causal relationship between multiple rounds of polyploidization and the functional divergence of duplicated genes is poorly understood. We focused on the Triticum–Aegilops complex lineage and compared selection pressure before and after tetraploidization and hexaploidization events. Although both events led to decreased selection pressure on homoeologous gene pairs (compared with diploids and tetraploids), the initial tetraploidization had a greater impact on selection pressure on homoeologous gene pairs than did subsequent hexaploidization. Consistent with this, selection pressure on expression patterns for the initial event relaxed more than those for the subsequent event. Surprisingly, the decreased selection pressure on these homoeologous genes was independent of the existence of in‐paralogs within the same subgenome. Wheat homoeologous pairs had different evolutionary consequences compared with orthologs related to other mechanisms (ancient allopolyploidization, ancient autopolyploidization, and small‐scale duplication). Furthermore, tetraploidization and hexaploidization also seemed to have different evolutionary consequences. This suggests that homoeologous genes retain unique functions, including functions that are unlikely to be preserved in genes generated by the other duplication mechanisms. We found that their unique functions differed between tetraploidization and hexaploidization (e.g., reproductive and chromosome segregation processes). These findings imply that the substantial number of gene pairs resulting from multiple allopolyploidization events, especially initial tetraploidization, may have been a unique source of functional divergence.

    DOI： 10.1111/tpj.17047

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  • Genome Editing to Produce Knockout Mutations of Seed Dormancy Genes in Wheat. Reviewed International journal

    Fumitaka Abe, Yoko Kamiya, Yuji Ishida, Hiroshi Hisano, Kanako Kawaura, Toshihiko Komari, Kazuhiro Sato

    Methods in molecular biology (Clifton, N.J.)   2830   137 - 148   2024

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    Language：English   Publishing type：Research paper (scientific journal)  

    Knockout mutants provide definitive information about the functions of genes related to agronomic traits, including seed dormancy. However, it takes many years to produce knockout mutants using conventional techniques in polyploid plants such as hexaploid wheat. Genome editing with sequence-specific nucleases is a promising approach for obtaining knockout mutations in all targeted homoeologs of wheat simultaneously. Here, we describe a procedure to produce a triple recessive mutant in wheat via genome editing. This protocol covers the evaluation of gRNA and Agrobacterium-mediated transformation to obtain edited wheat seedlings.

    DOI： 10.1007/978-1-0716-3965-8_13

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  • Characterization of alpha-gliadin alleles of Japanese wheat cultivars in relation to flour dough extensibility and celiac disease epitopes Reviewed

    Satoshi Noma, Miki Yamagishi, Yasunari Ogihara, Kanako Kawaura

    JOURNAL OF CEREAL SCIENCE   109   2023.1

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jcs.2022.103591

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  • Optimizing genome editing efficiency in wheat: Effects of heat treatments and different promoters for single guide RNA expression Reviewed

    Mitsuko Kishi-Kaboshi, Fumitaka Abe, Yoko Kamiya, Kanako Kawaura, Hiroshi Hisano, Kazuhiro Sato

    Plant Biotechnology   40 ( 3 )   237 - 245   2023

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    Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Plant Cell and Molecular Biology  

    DOI： 10.5511/plantbiotechnology.23.0717a

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  • Ethanol-Mediated Novel Survival Strategy against Drought Stress in Plants Reviewed

    Khurram Bashir, Daisuke Todaka, Sultana Rasheed, Akihiro Matsui, Zarnab Ahmad, Kaori Sako, Yoshinori Utsumi, Anh Thu Vu, Maho Tanaka, Satoshi Takahashi, Junko Ishida, Yuuri Tsuboi, Shunsuke Watanabe, Yuri Kanno, Eigo Ando, Kwang-Chul Shin, Makoto Seito, Hinata Motegi, Muneo Sato, Rui Li, Saya Kikuchi, Miki Fujita, Miyako Kusano, Makoto Kobayashi, Yoshiki Habu, Atsushi J Nagano, Kanako Kawaura, Jun Kikuchi, Kazuki Saito, Masami Yokota Hirai, Mitsunori Seo, Kazuo Shinozaki, Toshinori Kinoshita, Motoaki Seki

    Plant and Cell Physiology   63 ( 9 )   1181 - 1192   2022.8

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    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    Water scarcity is a serious agricultural problem causing significant losses to crop yield and product quality. The development of technologies to mitigate the damage caused by drought stress is essential for ensuring a sustainable food supply for the increasing global population. We herein report that the exogenous application of ethanol, an inexpensive and environmentally friendly chemical, significantly enhances drought tolerance in Arabidopsis thaliana, rice and wheat. The transcriptomic analyses of ethanol-treated plants revealed the upregulation of genes related to sucrose and starch metabolism, phenylpropanoids and glucosinolate biosynthesis, while metabolomic analysis showed an increased accumulation of sugars, glucosinolates and drought-tolerance-related amino acids. The phenotyping analysis indicated that drought-induced water loss was delayed in the ethanol-treated plants. Furthermore, ethanol treatment induced stomatal closure, resulting in decreased transpiration rate and increased leaf water contents under drought stress conditions. The ethanol treatment did not enhance drought tolerance in the mutant of ABI1, a negative regulator of abscisic acid (ABA) signaling in Arabidopsis, indicating that ABA signaling contributes to ethanol-mediated drought tolerance. The nuclear magnetic resonance analysis using 13C-labeled ethanol indicated that gluconeogenesis is involved in the accumulation of sugars. The ethanol treatment did not enhance the drought tolerance in the aldehyde dehydrogenase (aldh) triple mutant (aldh2b4/aldh2b7/aldh2c4). These results show that ABA signaling and acetic acid biosynthesis are involved in ethanol-mediated drought tolerance and that chemical priming through ethanol application regulates sugar accumulation and gluconeogenesis, leading to enhanced drought tolerance and sustained plant growth. These findings highlight a new survival strategy for increasing crop production under water-limited conditions.

    DOI： 10.1093/pcp/pcac114

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    Other Link： https://academic.oup.com/pcp/article-pdf/63/9/1181/45852399/pcac114.pdf

  • Comparative transcriptome analysis of synthetic and common wheat in response to salt stress Reviewed

    Rio Nakayama, Mohammad Taheb Safi, Waisuddin Ahmadzai, Kazuhiro Sato, Kanako Kawaura

    Scientific Reports   12 ( 1 )   11534   2022.7

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    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    Salt stress reduces wheat yield. Therefore, improvement for enhanced salt stress tolerance is necessary for stable production. To understand the molecular mechanism of salt tolerance in common wheat and synthetic hexaploid (SH) wheat, RNA sequencing was performed on the roots of three wheat lines salt-tolerant SH wheat, salt-tolerant common wheat, and salt-sensitive common wheat. Differentially expressed genes (DEGs) in response to salt stress were characterized using gene ontology enrichment analysis. Salt tolerance in common wheat has been suggested to be mainly regulated by the activation of transporters. In contrast, salt tolerance in SH wheat is enhanced through up-regulation of the reactive oxygen species signaling pathway, other unknown pathways, and different ERF transcription factors. These results indicate that salt tolerance is differentially controlled between common wheat and SH wheat. Furthermore, QTL analysis was performed using the F<sub>2</sub> population derived from SH and salt-sensitive wheat. No statistically significant QTL was detected, suggesting that numerous QTLs with negligible contributions are involved in salt tolerance in SH wheat. We also identified DEGs specific to each line near one probable QTL. These findings show that SH wheat possesses salt tolerance mechanisms lacking in common wheat and may be potential breeding material for salt tolerance.

    DOI： 10.1038/s41598-022-15733-2

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    Other Link： https://www.nature.com/articles/s41598-022-15733-2

  • Ethanol induces heat tolerance in plants by stimulating unfolded protein response. Reviewed International journal

    Matsui A, Todaka D, Tanaka M, Mizunashi K, Takahashi S, Sunaoshi Y, Tsuboi Y, Ishida J, Bashir K, Kikuchi J, Kusano M, Kobayashi M, Kawaura K, Seki M

    Plant molecular biology   110 ( 1-2 )   131 - 145   2022.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    KEY MESSAGE: Ethanol priming induces heat stress tolerance by the stimulation of unfolded protein response. Global warming increases the risk of heat stress-related yield losses in agricultural crops. Chemical priming, using safe agents, that can flexibly activate adaptive regulatory responses to adverse conditions, is a complementary approach to genetic improvement for stress adaptation. In the present study, we demonstrated that pretreatment of Arabidopsis with a low concentration of ethanol enhances heat tolerance without suppressing plant growth. We also demonstrated that ethanol pretreatment improved leaf growth in lettuce (Lactuca sativa L.) plants grown in the field conditions under high temperatures. Transcriptome analysis revealed a set of genes that were up-regulated in ethanol-pretreated plants, relative to water-pretreated controls. Binding Protein 3 (BIP3), an endoplasmic reticulum (ER)-stress marker chaperone gene, was among the identified up-regulated genes. The expression levels of BIP3 were confirmed by RT-qPCR. Root-uptake of ethanol was metabolized to organic acids, nucleic acids, amines and other molecules, followed by an increase in putrescine content, which substantially promoted unfolded protein response (UPR) signaling and high-temperature acclimation. We also showed that inhibition of polyamine production and UPR signaling negated the heat stress tolerance induced by ethanol pretreatment. These findings collectively indicate that ethanol priming activates UPR signaling via putrescine accumulation, leading to enhanced heat stress tolerance. The information gained from this study will be useful for establishing ethanol-mediated chemical priming strategies that can be used to help maintain crop production under heat stress conditions.

    DOI： 10.1007/s11103-022-01291-8

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  • Multiple Wheat Genomes Reveal Novel Gli-2 Sublocus Location and Variation of Celiac Disease Epitopes in Duplicated α-Gliadin Genes Reviewed

    Gwyneth Halstead-Nussloch, Tsuyoshi Tanaka, Dario Copetti, Timothy Paape, Fuminori Kobayashi, Masaomi Hatakeyama, Hiroyuki Kanamori, Jianzhong Wu, Martin Mascher, Kanako Kawaura, Kentaro K. Shimizu, Hirokazu Handa

    Frontiers in Plant Science   12   2021.9

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    Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    The seed protein α-gliadin is a major component of wheat flour and causes gluten-related diseases. However, due to the complexity of this multigene family with a genome structure composed of dozens of copies derived from tandem and genome duplications, little was known about the variation between accessions, and thus little effort has been made to explicitly target α-gliadin for bread wheat breeding. Here, we analyzed genomic variation in α-gliadins across 11 recently published chromosome-scale assemblies of hexaploid wheat, with validation using long-read data. We unexpectedly found that the <italic>Gli-B2</italic> locus is not a single contiguous locus but is composed of two subloci, suggesting the possibility of recombination between the two during breeding. We confirmed that the number of immunogenic epitopes among 11 accessions varied. The D subgenome of a European spelt line also contained epitopes, in agreement with its hybridization history. Evolutionary analysis identified amino acid sites under diversifying selection, suggesting their functional importance. The analysis opens the way for improved grain quality and safety through wheat breeding.

    DOI： 10.3389/fpls.2021.715985

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  • Geographical distribution and adaptive variation of VRN-A3 alleles in worldwide polyploid wheat (Triticum spp.) species collection. Reviewed International journal

    Kazusa Nishimura, Hirokazu Handa, Naoki Mori, Kanako Kawaura, Akira Kitajima, Tetsuya Nakazaki

    Planta   253 ( 6 )   132 - 132   2021.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    MAIN CONCLUSION: The distribution of early flowering alleles of VRN-A3 was found to be biased to low latitudes, and these alleles may contribute to environmental adaptability to low latitudes in cultivated emmer wheat. In wheat (Triticum spp.), the flowering time is an important trait for successful seed production and yield by adapting to the regional environment. An early flowering allele of VRN-A3 with 7- and 25-bp insertions in the promoter region (Vrn-A3a-h1) has recently been reported from the analysis of an emmer wheat (Triticum turgidum L. ssp. dicoccum) accession, TN26. This early flowering allele of VRN-A3 might be associated with the regional adaptation of wheat. In this study, we elucidated its geographic distribution to assess the importance of the early flowering allele of VRN-A3 in worldwide wheat collection. From sequence analysis, we identified six VRN-A3 alleles with the 7- and 25-bp insertions, namely, Vrn-A3a-h2, Vrn-A3a-h3, Vrn-A3a-h4, Vrn-A3a-h5, Vrn-A3a-h6, and Vrn-A3c-h2 from wild emmer wheat, while we identified two VRN-A3 alleles with these insertions, Vrn-A3a-h2 and Vrn-A3c-h1 from cultivated tetraploid and hexaploid wheat species in addition to Vrn-A3a-h1. Among VRN-A3 alleles distributed in cultivated wheat, we found that Vrn-A3a-h2 promoted early heading, whereas Vrn-A3c-h1 did not affect heading time. Our analysis showed that the distribution of early flowering alleles of VRN-A3 dominated in cultivated emmer wheat in Ethiopia and India, which actually showed an early flowering phenotype. This implied that the early flowering alleles of VRN-A3 contribute to adaptability to a low-latitude environment in cultivated emmer wheat. We could not find durum (T. turgidum L. ssp. durum) and bread wheat (T. aestivum L. ssp. aestivum) accessions with these early flowering alleles. Our findings indicated that Vrn-A3a-h1 and Vrn-A3a-h2 were useful for breeding of early flowering cultivars in durum and bread wheat varieties.

    DOI： 10.1007/s00425-021-03646-9

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  • In planta Genome Editing in Commercial Wheat Varieties. Reviewed International journal

    Yuelin Liu, Weifeng Luo, Qianyan Linghu, Fumitaka Abe, Hiroshi Hisano, Kazuhiro Sato, Yoko Kamiya, Kanako Kawaura, Kazumitsu Onishi, Masaki Endo, Seiichi Toki, Haruyasu Hamada, Yozo Nagira, Naoaki Taoka, Ryozo Imai

    Frontiers in plant science   12   648841 - 648841   2021

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    Language：English   Publishing type：Research paper (scientific journal)  

    Limitations for the application of genome editing technologies on elite wheat (Triticum aestivum L.) varieties are mainly due to the dependency on in vitro culture and regeneration capabilities. Recently, we developed an in planta particle bombardment (iPB) method which has increased process efficiency since no culture steps are required to create stably genome-edited wheat plants. Here, we report the application of the iPB method to commercially relevant Japanese elite wheat varieties. The biolistic delivery of gold particles coated with plasmids expressing CRISPR/Cas9 components designed to target TaQsd1 were bombarded into the embryos of imbibed seeds with their shoot apical meristem (SAM) exposed. Mutations in the target gene were subsequently analyzed within flag leaf tissue by using cleaved amplified polymorphic sequence (CAPS) analysis. A total of 9/358 (2.51%) of the bombarded plants (cv. "Haruyokoi," spring type) carried mutant alleles in the tissue. Due to the chimeric nature of the T0 plants, only six of them were inherited to the next (T1) generation. Genotypic analysis of the T2 plants revealed a single triple-recessive homozygous mutant of the TaQsd1 gene. Compared to wild type, the homozygous mutant exhibited a 7 days delay in the time required for 50% seed germination. The iPB method was also applied to two elite winter cultivars, "Yumechikara" and "Kitanokaori," which resulted in successful genome editing at slightly lower efficiencies as compared to "Haruyokoi." Taken together, this report demonstrates that the in planta genome editing method through SAM bombardment can be applicable to elite wheat varieties that are otherwise reluctant to callus culture.

    DOI： 10.3389/fpls.2021.648841

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  • Multiple wheat genomes reveal global variation in modern breeding. Reviewed International journal

    Sean Walkowiak, Liangliang Gao, Cecile Monat, Georg Haberer, Mulualem T Kassa, Jemima Brinton, Ricardo H Ramirez-Gonzalez, Markus C Kolodziej, Emily Delorean, Dinushika Thambugala, Valentyna Klymiuk, Brook Byrns, Heidrun Gundlach, Venkat Bandi, Jorge Nunez Siri, Kirby Nilsen, Catharine Aquino, Axel Himmelbach, Dario Copetti, Tomohiro Ban, Luca Venturini, Michael Bevan, Bernardo Clavijo, Dal-Hoe Koo, Jennifer Ens, Krystalee Wiebe, Amidou N'Diaye, Allen K Fritz, Carl Gutwin, Anne Fiebig, Christine Fosker, Bin Xiao Fu, Gonzalo Garcia Accinelli, Keith A Gardner, Nick Fradgley, Juan Gutierrez-Gonzalez, Gwyneth Halstead-Nussloch, Masaomi Hatakeyama, Chu Shin Koh, Jasline Deek, Alejandro C Costamagna, Pierre Fobert, Darren Heavens, Hiroyuki Kanamori, Kanako Kawaura, Fuminori Kobayashi, Ksenia Krasileva, Tony Kuo, Neil McKenzie, Kazuki Murata, Yusuke Nabeka, Timothy Paape, Sudharsan Padmarasu, Lawrence Percival-Alwyn, Sateesh Kagale, Uwe Scholz, Jun Sese, Philomin Juliana, Ravi Singh, Rie Shimizu-Inatsugi, David Swarbreck, James Cockram, Hikmet Budak, Toshiaki Tameshige, Tsuyoshi Tanaka, Hiroyuki Tsuji, Jonathan Wright, Jianzhong Wu, Burkhard Steuernagel, Ian Small, Sylvie Cloutier, Gabriel Keeble-Gagnère, Gary Muehlbauer, Josquin Tibbets, Shuhei Nasuda, Joanna Melonek, Pierre J Hucl, Andrew G Sharpe, Matthew Clark, Erik Legg, Arvind Bharti, Peter Langridge, Anthony Hall, Cristobal Uauy, Martin Mascher, Simon G Krattinger, Hirokazu Handa, Kentaro K Shimizu, Assaf Distelfeld, Ken Chalmers, Beat Keller, Klaus F X Mayer, Jesse Poland, Nils Stein, Curt A McCartney, Manuel Spannagl, Thomas Wicker, Curtis J Pozniak

    Nature   588 ( 7837 )   277 - 283   2020.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    Advances in genomics have expedited the improvement of several agriculturally important crops but similar efforts in wheat (Triticum spp.) have been more challenging. This is largely owing to the size and complexity of the wheat genome1, and the lack of genome-assembly data for multiple wheat lines2,3. Here we generated ten chromosome pseudomolecule and five scaffold assemblies of hexaploid wheat to explore the genomic diversity among wheat lines from global breeding programs. Comparative analysis revealed extensive structural rearrangements, introgressions from wild relatives and differences in gene content resulting from complex breeding histories aimed at improving adaptation to diverse environments, grain yield and quality, and resistance to stresses4,5. We provide examples outlining the utility of these genomes, including a detailed multi-genome-derived nucleotide-binding leucine-rich repeat protein repertoire involved in disease resistance and the characterization of Sm16, a gene associated with insect resistance. These genome assemblies will provide a basis for functional gene discovery and breeding to deliver the next generation of modern wheat cultivars.

    DOI： 10.1038/s41586-020-2961-x

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  • De Novo Genome Assembly of the Japanese Wheat Cultivar Norin 61 Highlights Functional Variation in Flowering Time and Fusarium Resistance Genes in East Asian Genotypes. Reviewed

    Kentaro K Shimizu, Dario Copetti, Moeko Okada, Thomas Wicker, Toshiaki Tameshige, Masaomi Hatakeyama, Rie Shimizu-Inatsugi, Catharine Aquino, Kazusa Nishimura, Fuminori Kobayashi, Kazuki Murata, Tony Kuo, Emily Delorean, Jesse Poland, Georg Haberer, Manuel Spannagl, Klaus F X Mayer, Juan Gutierrez-Gonzalez, Gary J Muehlbauer, Cecile Monat, Axel Himmelbach, Sudharsan Padmarasu, Martin Mascher, Sean Walkowiak, Tetsuya Nakazaki, Tomohiro Ban, Kanako Kawaura, Hiroyuki Tsuji, Curtis Pozniak, Nils Stein, Jun Sese, Shuhei Nasuda, Hirokazu Handa

    Plant & cell physiology   62 ( 1 )   8 - 27   2020.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    Bread wheat is a major crop that has long been the focus of basic and breeding research. Assembly of its genome has been difficult because of its large size and allohexaploid nature (AABBDD genome). Following the first reported assembly of the genome of the experimental strain Chinese Spring (CS), the 10+ Wheat Genomes Project was launched to produce multiple assemblies of worldwide modern cultivars. The only Asian cultivar in the project is Norin 61, a representative Japanese cultivar adapted to grow across a broad latitudinal range, mostly characterized by a wet climate and a short growing season. Here, we characterize key aspects of its chromosome-scale genome assembly spanning 15 Gb with a raw scaffold N50 of 23 Mb. Analysis of the repetitive elements identified chromosomal regions unique to Norin 61 that encompass a tandem array of the pathogenesis-related-13 family. We report novel copy-number variations in the B homeolog of the florigen gene FT1/VRN3, pseudogenization of its D homeolog, and the association of its A homeologous alleles with the spring/winter growth habit. Further, the Norin 61 genome carries typical East Asian functional variants from CS ranging from a single nucleotide to multi-Mb scale. Examples of such variation are the Fhb1 locus, which confers Fusarium head-blight resistance, Ppd-D1a, which confers early flowering, Glu-D1f for Asian noodle quality, and Rht-D1b, which introduced semi-dwarfism during the green revolution. The adoption of Norin 61 as a reference assembly for functional and evolutionary studies will enable comprehensive characterization of the underexploited Asian bread wheat diversity.

    DOI： 10.1093/pcp/pcaa152

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  • A rapid method for detection of mutations induced by CRISPR/Cas9-based genome editing in common wheat Reviewed

    Yoko Kamiya, Fumitaka Abe, Masafumi Mikami, Masaki Endo, Kanako Kawaura

    PLANT BIOTECHNOLOGY   37 ( 2 )   247 - 251   2020.6

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.5511/plantbiotechnology.20.0404b

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  • Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat. Reviewed International journal

    Fumitaka Abe, Emdadul Haque, Hiroshi Hisano, Tsuyoshi Tanaka, Yoko Kamiya, Masafumi Mikami, Kanako Kawaura, Masaki Endo, Kazumitsu Onishi, Takeshi Hayashi, Kazuhiro Sato

    Cell reports   28 ( 5 )   1362 - 1369   2019.7

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    Common wheat has three sets of sub-genomes, making mutations difficult to observe, especially for traits controlled by recessive genes. Here, we produced hexaploid wheat lines with loss of function of homeoalleles of Qsd1, which controls seed dormancy in barley, by Agrobacterium-mediated CRISPR/Cas9. Of the eight transformed wheat events produced, three independent events carrying multiple mutations in wheat Qsd1 homeoalleles were obtained. Notably, one line had mutations in every homeoallele. We crossed this plant with wild-type cultivar Fielder to generate a transgene-free triple-recessive mutant, as revealed by Mendelian segregation. The mutant showed a significantly longer seed dormancy period than wild-type, which may result in reduced pre-harvest sprouting of grains on spikes. PCR, southern blotting, and whole-genome shotgun sequencing revealed that this segregant lacked transgenes in its genomic sequence. This technique serves as a model for trait improvement in wheat, particularly for genetically recessive traits, based on locus information from diploid barley.

    DOI： 10.1016/j.celrep.2019.06.090

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  • Contribution of α-gliadin alleles to the extensibility of flour dough in Japanese wheat cultivars Reviewed

    Noma S, Hayakawa K, Abe C, Suzuki S, Kawaura K

    Journal of Cereal Science   86   15 - 21   2019.3

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  • Molecular characterization of gliadins of Chinese Spring wheat in relation to celiac disease elicitors. Reviewed

    Kawaura K, Miura M, Kamei Y, Ikeda TM, Ogihara Y

    Genes & genetic systems   93 ( 1 )   9 - 20   2018.1

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    DOI： 10.1266/ggs.17-00034

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  • Transcriptome of Homoeologous Genes Deduced from the Full-Length cDNA Clones of Common Wheat, Triticum aestivum L Reviewed

    Mishina K, Kawaura K, Kamiya Y, Kajita Y, Mochida K, Tarui H, Tagami M, Suzuki N, Kawai J, Nakamura Y, Yano K, Ogihara Y

    Journal of Genetics & Genomic Sci   3   007   2018

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  • Acetate-mediated novel survival strategy against drought in plants Reviewed

    Jong-Myong Kim, Taiko Kim To, Akihiro Matsui, Keitaro Tanoi, Natsuko I. Kobayashi, Fumio Matsuda, Yoshiki Habu, Daisuke Ogawa, Takuya Sakamoto, Sachihiro Matsunaga, Khurram Bashir, Sultana Rasheed, Marina Ando, Hiroko Takeda, Kanako Kawaura, Miyako Kusano, Atsushi Fukushima, Takaho A. Endo, Takashi Kuromori, Junko Ishida, Taeko Morosawa, Maho Tanaka, Chieko Torii, Yumiko Takebayashi, Hitoshi Sakakibara, Yasunari Ogihara, Kazuki Saito, Kazuo Shinozaki, Alessandra Devoto, Motoaki Seki

    NATURE PLANTS   3 ( 7 )   17097   2017.7

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    DOI： 10.1038/nplants.2017.97

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  • A Causal Gene for Seed Dormancy on Wheat Chromosome 4A Encodes a MAP Kinase Kinase Reviewed

    Atsushi Torada, Michiya Koike, Taiichi Ogawa, Yu Takenouchi, Kazuki Tadamura, Jianzhong Wu, Takashi Matsumoto, Kanako Kawaura, Yasunari Ogihara

    CURRENT BIOLOGY   26 ( 6 )   782 - 787   2016.3

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    DOI： 10.1016/j.cub.2016.01.063

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  • Comprehensive molecular characterization of the alpha/beta-gliadin multigene family in hexaploid wheat Reviewed

    Satoshi Noma, Kanako Kawaura, Katsuyuki Hayakawa, Chikako Abe, Noritaka Tsuge, Yasunari Ogihara

    MOLECULAR GENETICS AND GENOMICS   291 ( 1 )   65 - 77   2016.2

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    DOI： 10.1007/s00438-015-1086-7

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  • TaqSH1-D, wheat ortholog of rice seed shattering gene qSH1, maps to the interval of a rachis fragility QTL on chromosome 3DL of common wheat (Triticum aestivum) Reviewed

    Mazen Katkout, Shun Sakuma, Kanako Kawaura, Yasunari Ogihara

    GENETIC RESOURCES AND CROP EVOLUTION   62 ( 7 )   979 - 984   2015.10

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    DOI： 10.1007/s10722-015-0301-z

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  • Comparison of genome-wide gene expression patterns in the seedlings of nascent allohexaploid wheats produced by two combinations of hybrids. Reviewed

    Jung Y, Kawaura K, Kishii M, Sakuma S, Ogihara Y

    Genes & genetic systems   90 ( 2 )   79 - 88   2015

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  • Changes in genome-wide gene expression during allopolyploidization and genome stabilization in hexaploid wheat Reviewed

    Yeonju Jung, Kanako Kawaura, Kohei Mishina, Shun Sakuma, Masahiro Kishii, Yasunari Ogihara

    GENES & GENETIC SYSTEMS   89 ( 5 )   215 - 225   2014.10

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    DOI： 10.1266/ggs.89.215

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  • QTL analysis of genetic loci affecting domestication-related spike characters in common wheat Reviewed

    Mazen Katkout, Masahiro Kishii, Kanako Kawaura, Kouhei Mishina, Shun Sakuma, Kazuko Umeda, Shigeo Takumi, Miyuki Nitta, Shuhei Nasuda, Yasunari Ogihara

    GENES & GENETIC SYSTEMS   89 ( 3 )   121 - 131   2014.6

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    DOI： 10.1266/ggs.89.121

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  • Identification of a novel homolog for a calmodulin-binding protein that is upregulated in alloplasmic wheat showing pistillody Reviewed

    Mika Yamamoto, Naoki Shitsukawa, Maki Yamada, Keisuke Kato, Shigeo Takumi, Kanako Kawaura, Yasunari Ogihara, Koji Murai

    PLANTA   237 ( 4 )   1001 - 1013   2013.4

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    DOI： 10.1007/s00425-012-1812-x

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  • Effects of barley chromosome addition to wheat on behavior and development of Locusta migratoria nymphs Reviewed

    Shunji Suematsu, Ken-Ichi Harano, Seiji Tanaka, Kanako Kawaura, Yasunari Ogihara, Yasuhiko Watari, Osamu Saito, Makoto Tokuda

    Scientific Reports   3   2577   2013

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    DOI： 10.1038/srep02577

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  • Genome change in wheat observed through the structure and expression of alpha/beta-gliadin genes Reviewed

    K. Kawaura, J. Wu, T. Matsumoto, H. Kanamori, S. Katagiri, Y. Ogihara

    FUNCTIONAL & INTEGRATIVE GENOMICS   12 ( 2 )   341 - 355   2012.6

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    DOI： 10.1007/s10142-012-0269-0

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  • Comprehensive Functional Analyses of Expressed Sequence Tags in Common Wheat (Triticum aestivum) Reviewed

    Alagu Manickavelu, Kanako Kawaura, Kazuko Oishi, Tadasu Shin-I, Yuji Kohara, Nabila Yahiaoui, Beat Keller, Reina Abe, Ayako Suzuki, Taishi Nagayama, Kentaro Yano, Yasunari Ogihara

    DNA RESEARCH   19 ( 2 )   165 - 177   2012.4

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    DOI： 10.1093/dnares/dss001

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  • Overexpression of Q/q-related homoeoalleles of hexaploid wheat reveals distinct recovery of flower transformation in the apetala2 mutant of Arabidopsis Reviewed

    Parisa Abdollahi, Yoko Kamiya, Kanako Kawaura, Yasunari Ogihara

    PLANT BIOTECHNOLOGY   29 ( 3 )   245 - 252   2012

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    DOI： 10.5511/plantbiotechnology.12.0224a

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  • Effect of barley chromosome addition on the susceptibility of wheat to feeding by a gall-inducing leafhopper Reviewed

    Shun Kumashiro, Keiichiro Matsukura, Kanako Kawaura, Masaya Matsumura, Yasunari Ogihara, Makoto Tokuda

    NATURWISSENSCHAFTEN   98 ( 11 )   983 - 987   2011.11

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    DOI： 10.1007/s00114-011-0846-4

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  • A New Insight into Application for Barley Chromosome Addition Lines of Common Wheat: Achievement of Stigmasterol Accumulation Reviewed

    Jianwei Tang, Kiyoshi Ohyama, Kanako Kawaura, Hiromi Hashinokuchi, Yoko Kamiya, Masashi Suzuki, Toshiya Muranaka, Yasunari Ogihara

    PLANT PHYSIOLOGY   157 ( 3 )   1555 - 1567   2011.11

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    DOI： 10.1104/pp.111.183533

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  • A Wheat Homolog of MOTHER OF FT AND TFL1 Acts in the Regulation of Germination Reviewed

    Shingo Nakamura, Fumitaka Abe, Hiroyuki Kawahigashi, Kou Nakazono, Akemi Tagiri, Takashi Matsumoto, Shigeko Utsugi, Taiichi Ogawa, Hirokazu Handa, Hiroki Ishida, Masahiko Mori, Kanako Kawaura, Yasunari Ogihara, Hideho Miura

    PLANT CELL   23 ( 9 )   3215 - 3229   2011.9

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    DOI： 10.1105/tpc.111.088492

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  • Heteroplasmy and expression of mitochondrial genes in alloplasmic and euplasmic wheat Reviewed

    Kanako Kawaura, Asuka Saeki, Takehiro Masumura, Shigeto Morita, Yasunari Ogihara

    GENES & GENETIC SYSTEMS   86 ( 4 )   249 - 255   2011.8

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    DOI： 10.1266/ggs.86.249

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  • Molecular mapping of quantitative trait loci for domestication traits and beta-glucan content in a wheat recombinant inbred line population Reviewed

    Alagu Manickavelu, Kanako Kawaura, Hisako Imamura, Michiko Mori, Yasunari Ogihara

    EUPHYTICA   177 ( 2 )   179 - 190   2011.1

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    DOI： 10.1007/s10681-010-0217-9

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  • Comparative Gene Expression Analysis of Susceptible and Resistant Near-Isogenic Lines in Common Wheat Infected by Puccinia triticina Reviewed

    Alagu Manickavelu, Kanako Kawaura, Kazuko Oishi, Tadasu Shin-I, Yuji Kohara, Nabila Yahiaoui, Beat Keller, Ayako Suzuki, Kentaro Yano, Yasunari Ogihara

    DNA RESEARCH   17 ( 4 )   211 - 222   2010.8

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    DOI： 10.1093/dnares/dsq009

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  • Assessment of adaptive evolution between wheat and rice as deduced from full-length common wheat cDNA sequence data and expression patterns Reviewed

    Kanako Kawaura, Keiichi Mochida, Akiko Enju, Yasushi Totoki, Atsushi Toyoda, Yoshiyuki Sakaki, Chikatoshi Kai, Jun Kawai, Yoshihide Hayashizaki, Motoaki Seki, Kazuo Shinozaki, Yasunari Ogihara

    BMC GENOMICS   10   271   2009.6

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    DOI： 10.1186/1471-2164-10-271

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  • Genome-wide analysis for identification of salt-responsive genes in common wheat Reviewed

    Kanako Kawaura, Keiichi Mochida, Yasunari Ogihara

    FUNCTIONAL & INTEGRATIVE GENOMICS   8 ( 3 )   277 - 286   2008.8

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    DOI： 10.1007/s10142-008-0076-9

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  • Development of abiotic stress tolerance via bZIP-type transcription factor LIP19 in common wheat Reviewed

    Fuminori Kobayashi, Eri Maeta, Akihiro Terashima, Kanako Kawaura, Yasunari Ogihara, Shigeo Takumi

    JOURNAL OF EXPERIMENTAL BOTANY   59 ( 4 )   891 - 905   2008.3

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    DOI： 10.1093/jxb/ern014

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  • Structures of the three homoeologous loci of wheat benzoxazinone biosynthetic genes TaBx3 and TaBx4 and characterization of their promoter sequences Reviewed

    Taiji Nomura, Shuhei Nasuda, Kanako Kawaura, Yasunari Ogihara, Nobuhiko Kato, Fumihiko Sato, Toshio Kojima, Atsushi Toyoda, Hajime Iwamura, Takashi R. Endo

    THEORETICAL AND APPLIED GENETICS   116 ( 3 )   373 - 381   2008.2

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    DOI： 10.1007/s00122-007-0675-1

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  • Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat Reviewed

    Naoki Shitsukawa, Chikako Tahira, Ken-ichiro Kassai, Chizuru Hirabayashi, Tomoaki Shimizu, Shigeo Takumi, Keiichi Mochida, Kanako Kawaura, Yasunari Ogihara, Koji Murai

    PLANT CELL   19 ( 6 )   1723 - 1737   2007.6

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    DOI： 10.1105/tpc.107.051813

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  • Sequence upstream of the wheat (Triticum aestivum L.) ALMT1 gene and its relationship to aluminum resistance Reviewed

    Takayuki Sasaki, Peter R. Ryan, Emmanuel Delhaize, Diane M. Hebb, Yasunari Ogihara, Kanako Kawaura, Kazuhiro Noda, Toshio Kojima, Atsushi Toyoda, Hideaki Matsumoto, Yoko Yamamoto

    PLANT AND CELL PHYSIOLOGY   47 ( 10 )   1343 - 1354   2006.10

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    DOI： 10.1093/pcp/pcl002

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  • Tissue expression map of a large number of expressed sequence tags and its application to in silico screening of stress response genes in common wheat Reviewed

    Keiichi Mochida, Kanako Kawaura, Etsuo Shimosaka, Naoto Kawakami, Tadasu Shin-, Yuji Kohara, Yukiko Yamazaki, Yasunari Ogihara

    MOLECULAR GENETICS AND GENOMICS   276 ( 3 )   304 - 312   2006.9

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    DOI： 10.1007/s00438-006-0120-1

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  • Isolation and characterization of SSR sequences from the genome and TAC clones of common wheat using the PCR technique Reviewed

    M Koike, K Kawaura, Y Ogihara, A Torada

    GENOME   49 ( 5 )   432 - 444   2006.5

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    DOI： 10.1139/G06-001

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  • Transcriptome analysis of salinity stress responses in common wheat using a 22k oligo-DNA microarray Reviewed

    Kanako Kawaura, Keiichi Mochida, Yukiko Yamazaki, Yasunari Ogihara

    Functional and Integrative Genomics   6 ( 2 )   132 - 142   2006.3

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    DOI： 10.1007/s10142-005-0010-3

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  • Expression profile of two storage-protein gene families in hexaploid wheat revealed by large-scale analysis of expressed sequence tags Reviewed

    K Kawaura, K Mochida, Y Ogihara

    PLANT PHYSIOLOGY   139 ( 4 )   1870 - 1880   2005.12

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    DOI： 10.1104/pp.105.070722

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  • Construction of a full-length cDNA library from young spikelets of hexaploid wheat and its characterization by large-scale sequencing of expressed sequence tags Reviewed

    Y Ogihara, K Mochida, K Kawaura, K Murai, M Seki, A Kamiya, K Shinozaki, P Carninci, Y Hayashizaki, T Shin-I, Y Kohara, Y Yamazaki

    GENES & GENETIC SYSTEMS   79 ( 4 )   227 - 232   2004.8

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    DOI： 10.1266/ggs.79.227

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  • Development of an efficient maintenance and screening system for large-insert genomic DNA libraries of hexaploid wheat in a transformation-competent artificial chromosome (TAC) vector Reviewed

    YG Liu, K Nagaki, M Fujita, K Kawaura, M Uozumi, Y Ogihara

    PLANT JOURNAL   23 ( 5 )   687 - 695   2000.9

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  • Identification of Marker Loci for Seed Dormancy in Rice (Oryza sativa L.) Reviewed

    J. Wan, T. Nakazaki, K. Kawaura, H. Ikehashi

    Crop Science   37 ( 6 )   1759 - 1763   1997.11

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    DOI： 10.2135/cropsci1997.0011183x003700060015x

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MISC

  • Effects of promoters for gRNA expression and cultivation temperatures on genome editing efficiency in wheat

    加星光子, 安倍史高, 神谷容子, 川浦香奈子, 久野裕, 佐藤和広

    育種学研究   24   2022

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  • Structural analysis of the Gli-2 loci for alpha-gliadin in cultivars of the international wheat 10+ genomes project

    半田裕一, 半田裕一, 清水健太郎, 清水健太郎, GWYNETH Halstead-Nussloch, 田中剛, TIMOTHY Paape, DARIO Copetti, 小林史典, 畠山剛臣, 畠山剛臣, 金森裕之, WU J., 川浦香奈子, POZNIAK Curtis J.

    育種学研究   22   2020

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  • de novo genome assembly of a Japanese wheat cultivar Norin 61 in the international wheat 10+ genomes project

    清水健太郎, 清水健太郎, 半田裕一, 半田裕一, 那須田周平, 瀬々潤, 瀬々潤, 川浦香奈子, 辻寛之, 爲重才覚, 坂智広, DARIO Copetti, 畠山剛臣, 畠山剛臣, 清水(稲継)理恵, CATHARINE Aquino, 小林史典, GUTIERREZ-GONZALEZ Juan, STEIN Nils, DELOREAN Emily, PAAPE Tim, HALSTEAD-NUSSLOCH Gwyneth, HABERER Georg, SPANNAGL Manuel, MAYER Klaus, MASCHER Martin, HIMMELBACH Axel, PADMARASU Sudharsan, WICKER Thomas, POZNIAK Curtis J.

    育種学研究   22   2020

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  • ゲノム編集技術による穂発芽耐性の改良されたコムギ育種素材

    安倍史高, ハク エムダドウル, 久野裕, 田中剛, 神谷容子, 三上雅史, 川浦香奈子, 遠藤真咲, 大西一光, 林武司, 佐藤和広

    農研機構次世代作物開発研究センター成果情報(Web)   2019   2019

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  • Molecular characterization of alpha/beta-gliadin multigene loci in hexaploid wheat as revealed by their organizations, expressions and evolutions

    Kanako Kawaura, Wu Jianzhong, Takashi Matsumoto, Hiroyuki Kanamori, Satoshi Katagiri, Yasunari Ogihara

    GENES & GENETIC SYSTEMS   86 ( 6 )   411 - 411   2011.12

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  • Effect of barley chromosome addition on the susceptibility of wheat to feeding by gall-inducing leafhopper, Cicadulina bipunctata (Hemiptera: Cicadellidae)

    S. Kumashiro, K. Matsukura, K. Kawaura, M. Matsumura, Y. Ogihara, M. Tokuda

    PHYTOPATHOLOGY   101 ( 6 )   S94 - S94   2011.6

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  • Genome organization of alpha/beta-gliadin multigenes in common wheat

    Kanako Kawaura, Wu Jianzhong, Takashi Matsumoto, Hiroyuki Kanamori, Satoshi Katagiri, Yasunari Ogihara

    GENES & GENETIC SYSTEMS   85 ( 6 )   428 - 428   2010.12

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  • Genetic and epigenetic alteration among three homoeologous genes of a MADS-box gene in hexaploid wheat

    Koji Murai, Naoki Shitsukawa, Shigeo Takumi, Keiichi Mochida, Kanako Kawaura, Yasunari Ogihara

    GENES & GENETIC SYSTEMS   82 ( 6 )   534 - 534   2007.12

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  • Silencing of paternally transmitted mitochondrial genes in alloplasmic wheat

    Yasunari Ogihara, Asuka Saeki, Shigeto Morita, Takehiro Masumura, Shigeru Satoh, Kanako Kawaura

    GENES & GENETIC SYSTEMS   82 ( 6 )   521 - 521   2007.12

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  • Microarray analysis of salt-responsive genes in common wheat

    Kanako Kawaura, Keiichi Mochida, Yasunari Ogihara

    GENES & GENETIC SYSTEMS   81 ( 6 )   437 - 437   2006.12

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  • Transcriptome analysis of wheat leaves developed at low temperature during long-terrn cold acclimation

    E Shimosaka, K Kawaura, Y Sato, Y Ogihara

    PLANT AND CELL PHYSIOLOGY   46   S118 - S118   2005

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  • Functional Genomics of Wheat. X. : Profiling of gene expression patterns as monitored by the full length cDNA microarray

    OGIHARA Y., MOCHIDA K., KAWAURA K., HIRANO R., TSUJIMOTO A.

    4   113 - 113   2002.8

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  • Systematic analysis of wheat gene expression during flowering stages as monitored by cDNA microarray system

    IIDA R., KAWAURA K., MURAI K., TSUJIMOTO A., OGIHARA Y.

    4 ( 1 )   47 - 47   2002.3

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Research Projects

  • パンコムギのアレルゲンとなるグルテンタンパク質を包括的に制御する転写因子の同定

    Grant number：22K05574  2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    川浦 香奈子

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    Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

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  • ゲノム編集によりグルテンを改変した低アレルゲン小麦の作出

    Grant number：19K05977  2019.4 - 2023.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    川浦 香奈子

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    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

    パンコムギにおける種子貯蔵タンパク質の多重遺伝子それぞれの発現を制御する転写因子を特定し、多重遺伝子の発現制御機構を明らかにすること、ゲノム編集技術を用いて転写因子の機能欠損体を作出し、低アレルゲン化したコムギの作出を目的とした。
    ゲノム編集を行うコムギ品種Fielderにおいて、種子貯蔵タンパク質遺伝子の発現を調節する転写因子として報告のあるSPA、SHP（O2-like）、およびWPBFの遺伝子発現パターンを調べた。また、種子貯蔵タンパク質の中で、ゲノム中にコピー数が比較的少ない高分子グルテニンサブユニット（HMW-GS）をコードする遺伝子について、Fielderにおけるコピー数と発現調節領域のシス配列をゲノムデータから調べた。転写因子とHMW-GSの遺伝子発現の発現パターンをqRT-PCRにより定量した結果、それぞれ種子登熟期の胚乳で発現しており、HMW-GSの発現とSPAとWPBFの発現に正の相関が見られたが、SHPは逆のパターンが見られた。また、転写因子の同祖遺伝子間で発現パターンが異なることが示された。
    パンコムギのCRISPR/Cas9システムによるゲノム編集の変異導入を行うため、前年度はRNPによる変異導入を目指したが、変異導入効率を上げることができなかったため、今年度はCas9とgRNAを発現するプラスミドベクターをパーティクルデリバリーシステムでコムギ未熟胚に導入した。標的配列の特異性による変異導入効率向上を目的とし、SPA、SHPについて標的配列の再検討を行った。プラスミドベクターを導入した未熟胚からDNAを抽出し、ゲノム編集効率を調べたところ、数％の変異導入が認められたため、今後は再生させ、ゲノム編集個体の評価を行っていく予定である。

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  • Molecular characterization of gliadins in aneuploid lines of Chinese Spring wheat

    Grant number：15K07261  2015.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Kawaura Kanako

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    Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）

    Seed storage proteins are encoded by multigenes in bread wheat. Gliadins are encoded by extremely large multigene family and some kinds of them cause allergy symptoms or celiac disease (CD) in human. To characterize distinct gliadins, two-dimensional electrophoresis was conducted using gliadins extracted from aneuploid lines of Chinese Spring wheat. Gliadins were separated into 90 spots and these spots were assigned to the chromosomal loci. Among lines, we found lines in which specific gliadins harboring CD epitopes were absent. Genomic PCR showed that these lines carry deletion of the chromosome segment containing the gliadin locus on the 6D chromosome. In addition, an antibody, which reacts the specific gliadins harboring CD epitopes, were designed and constructed. These liens and the antibody could be useful resource in breeding programs for reduction of CD immunotoxicity.

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  • Comprehensive analysis of wheat seed storage proteins associated with wheat allergy

    Grant number：24580011  2012.4 - 2015.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    KAWAURA Kanako

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    Grant amount：\5460000 （ Direct Cost: \4200000 、 Indirect Cost：\1260000 ）

    In order to screen for hypoallergenic wheat, seed storage proteins extracted from 265 lines of various hexaploid wheat were evaluated by Western blot using three kinds of anti-peptide antibodies for epitope of wheat allergy.
    Gliadins were selectively extracted from Chinese Spring wheat and its aneuploid lines. Two-dimensional electrophoresis profiles were compared among lines. Out of 70 gliadin spots, 52 spots could be determined their chromosome loci. Furthermore, gliadins which have the epitope of wheat allergy and are transcribed from chromosome 6D were specifically suppressed in the tetrasomic 2A lines, suggesting Chinese Spring wheat has a specific gliadin suppressor on chromosome 2A.

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  • Mechanism of feeding deterrence of barley seedlings against the migratory locust

    Grant number：24658054  2012.4 - 2014.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    TOKUDA Makoto, TANAKA Seiji, KAWAURA Kanako, JIKUMARU Yusuke

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    Grant amount：\4030000 （ Direct Cost: \3100000 、 Indirect Cost：\930000 ）

    Locusta migratoria feeds on various Poaceae but barley. Barley genes related to feeding deterrence are useful for developing novel resistant crops. We investigated the effects of barley cultivar Betzes, wheat cultivar Chinese Spring (CS), and six barley chromosome disomic addition lines of wheat (2H-7H) on locomotor activity, feeding behavior, survival and development of L. migratoria. Locomotor activity was similar in nymphs kept with wheat and 2H-7H in an actograph, whereas it was generally high in those kept with barely. Feeding tests suggested that barley genes related to inhibition of feeding by L. migratoria are located on barley chromosomes 5H and 6H and those related to the palatability of plants on chromosomes 2H, 5H and 6H. Rearing experiments suggested the presence of barley genes negatively affecting the survival and growth of locust nymphs on chromosomes 5H and 2H, respectively, and the effects are phase-dependent. The results were published in Suematsu et al. (2013).

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  • Identification of wheat gliadin genes related to allergy

    Grant number：22780006  2010 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    KAWAURA Kanako

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    Grant amount：\3640000 （ Direct Cost: \2800000 、 Indirect Cost：\840000 ）

    In order to measure the level of antigen-antibody reaction against wheat storage protein related to wheat allergy, three kinds of peptide polyclonal antibody were produced. Using the antibody, about 170 lines of wheat were screened by the western-blot analysis. Furthermore, to understand genome structure and the expression of allergen α/β-gliadin multigenes, genome analysis was conducted. Duplication of genome segments containing of α/β-gliadin genes brought about through unequal crossing-over or saltatory replication, and α/β-gliadin genes for their own were duplicated without any recombination events. They have similar cis-elements and promoter structures, the mechanisms underlying their distinct gene expression and possible applications were suggested.

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  • Clarification of the mechanism determining host and non-host plants in insects using wheat-barley chromosome addition lines

    Grant number：21200066  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

    TOKUDA Makoto, JIKUMARU Yusuke, KAWAURA Kanako, MATSUKURA Keiichirou, KAMIYA Yuji, OGIHARA Yasunari, MATSUMURA Masaya, TANAKA Seiji

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    Grant amount：\27430000 （ Direct Cost: \21100000 、 Indirect Cost：\6330000 ）

    We studied the feeding preference and developmental performance of Locusta migratoria and Cicadullina bipunctata on wheat-barley chromosome addition lines. We also searched for barley genes related to the inhibition of feeding by L. migratoria and to gall induction by C. bipunctata by ecological and molecular biological experiments.

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  • Analysis of sterol metabolic pathway in hexaploid wheat

    Grant number：21580080  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    MURANAKA Toshiya, NAGATA Noriko, ISSHIKI Masayuki, KAWAMURA Kanako, OYAMA Kiyoshi

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    Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）

    Wheat(Triticum aestivum) is one of the three major crops. It is expected to add value to the crop by the modification of sterol profiles. However, because of the complicated genome(hexaploid) and difficulty of transgenic work, there have been no information of sterol pathway in the crop. In this study, 13 genes for putative sterol metabolic pathway was identified. Among these genes, we elucidated the CYP710A8 encoding sterol C-22 desaturase as a key characteristic for the higher level of stigmasterol. The gene was mapped on chromosome 3(3A, 3B, and 3D) and difference of the function of the genes was confirmed by overexpression of each gene in Arabidopsis.

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  • Analysis of seed storage protein genes for improvement of gluten quality in bread wheat

    Grant number：19780005  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    KAWAURA Kanako

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    Grant amount：\3450000 （ Direct Cost: \3000000 、 Indirect Cost：\450000 ）

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