Updated on 2025/09/04

写真a

 
Robert A. Kanaly
 
Organization
Graduate School of Nanobioscience Department of Life and Environmental System Science Professor
School of Science Department of Science
Title
Professor
External link

Degree

  • Ph.D. ( Rutgers University, NJ, USA )

  • B.S. ( Rutgers University, NJ, USA )

Research Interests

  • Environmental Microbiology, Microbial Ecology

  • Mass spectrometry

  • DNA adductomics, RNA adductomics

  • Polycyclic aromatic hydrocarbons (PAHs)

  • Biodegradation, Biotransformation, Bioremediation of Hazardous Pollutants

  • Environmental Toxicology

Research Areas

  • Life Science / Ecology and environment  / Environmental Microbiology, Biotransformation, Bioremediation

Research History

  • Yokohama City University   Department of Life and Environmental Sciences   Professor

    2015

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  • Yokohama City University   Department of Life and Environmental Sciences   Associate Professor

    2006 - 2015

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  • Kyoto University   Graduate School of Global Environmental Studies   JSPS Research Fellow

    2004 - 2006

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Professional Memberships

  • The Japanese Society of Microbial Ecology (JSME)

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  • American Society for Microbiology, Division of Applied and Environmental Microbiology, Division of Microbial Ecology

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  • American Chemical Society (ACS), Division of Toxicology

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  • The Japanese Environmental Mutagen Society (JEMS)

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Papers

  • Utilization of the C9 aromatic hydrocarbon n-propylbenzene by Sphingobium barthaii KK22 and coexistence of multiple biodegradation pathways. Reviewed

    Sakai, M., Mori, J. F., Kanaly, R. A.

    Biodegradation   2025

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  • Transcriptomic profiling of Pseudomonas migulae revealed gene regulatory properties during biodegradation of aromatic hydrocarbons under cold stress Reviewed

    Yanagita, H., R. A. Kanaly, J. F. Mori

    Microbial Genomics   2025

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  • 1,6-Digalloyl glucose in Emblica officinalis increases the proliferation of human keratinocytes and fibroblasts with upregulated expression of periostin. Reviewed

    Yamakami, Y., Morino, K., Kanaly, R.A., Irie, R., Oikawa, M., Takauji, Y., Miki, K., Hossain, M., Ayusawa, D., Fujii, M.

    Bioscience, Biotechnology, and Biochemistry   2025

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  • Assessment of bacterial biotransformation of alkylnaphthalene lubricating base oil component 1-butylnaphthalene by LC/ESI-MS(/MS) Reviewed

    Sakai, M., J. F. Mori, R. A. Kanaly

    Chemosphere   364   2024

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.chemosphere.2024.143269

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  • Formation of biogenic manganese oxide nodules on hyphae of a new fungal isolate of Periconia that immobilizes aqueous copper Reviewed

    Tsushima, S., Y. Nishi, R. Suzuki, M. Tachibana, R. A. Kanaly, J. F. Mori

    Microbes and Environments   39   2024

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    Publishing type:Research paper (scientific journal)   Publisher:Japanese Society of Microbial Ecology  

    DOI: 10.1264/jsme2.me23102

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  • Identification of a putative novel polycyclic aromatic hydrocarbon-biodegrading gene cluster in a marine Roseobacteraceae bacterium Sagittula sp. MA-2 Reviewed

    Abe, M., Sakai, M., Kanaly, R. A., Mori, J. F.

    Microbiology Spectrum   13   2024

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  • Nondesulfurizing benzothiophene biotransformation to hetero and homodimeric ortho-substituted diaryl disulfides by the model PAH-degrading Sphingobium barthaii Reviewed

    Nemoto, Y, K. Ozawa, J.F. Mori, R.A. Kanaly

    Biodegradation   34   2023

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  • The GGDEF protein Dgc2 suppresses both motility and biofilm formation in the filamentous cyanobacterium <i>Leptolyngbya boryana</i> Reviewed

    Toida, K, W. Kushida, H. Yamamoto, K. Yamamoto, K. Ishii, K. Uesaka, R. A. Kanaly, S. Kutsuna, K. Ihara, Y. Fujita, H. Iwasaki

    Microbiology Spectrum   11   2023

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    Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    ABSTRACT

    Colony pattern formations of bacteria with motility manifest complicated morphological self-organization phenomena. Leptolyngbya boryana is a filamentous cyanobacterium, which has been used as a genetic model organism for studying metabolism including photosynthesis and nitrogen fixation. A widely used type strain [wild type (WT) in this article] of this species has not been reported to show any motile activity. However, we isolated a spontaneous mutant strain that shows active motility (gliding activity) to give rise to complicated colony patterns, including comet-like wandering clusters and disk-like rotating vortices on solid media. Whole-genome resequencing identified multiple mutations in the genome of the mutant strain. We confirmed that inactivation of the candidate gene dgc2 ( LBDG_02920 ) in the WT background was sufficient to give rise to motility and morphologically complex colony patterns. This gene encodes a protein containing the GGDEF motif which is conserved at the catalytic domain of diguanylate cyclase (DGC). Although DGC has been reported to be involved in biofilm formation, the dgc2 mutant significantly facilitated biofilm formation, suggesting a role for the dgc2 gene in suppressing both gliding motility and biofilm formation. Thus, Leptolyngbya is expected to be an excellent genetic model for studying dynamic colony pattern formation and to provide novel insights into the role of DGC family genes in biofilm formation.

    IMPORTANCE

    Self-propelled bacteria often exhibit complex collective behaviors, such as formation of dense-moving clusters, which are exemplified by wandering comet-like and rotating disk-like colonies; however, the molecular details of how these structures are formed are scant. We found that a strain of the filamentous cyanobacterium Leptolyngbya deficient in the GGDEF protein gene dgc2 elicits motility and complex and dynamic colony pattern formation, including comet-like and disk-like clusters. Although c-di-GMP has been reported to activate biofilm formation in some bacterial species, disruption of dgc2 unexpectedly enhanced it, suggesting a novel role for this GGDEF protein for inhibiting both colony pattern formation and biofilm formation.

    DOI: 10.1128/spectrum.04837-22

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  • Genomic analysis of a marine alphaproteobacterium Sagittula sp. strain MA-2 that carried eight plasmids Reviewed

    Abe, M, R. A. Kanaly, J. F. Mori

    Marine Genomics   72   2023

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.margen.2023.101070

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  • Whole-genome sequence of <i>Periconia</i> sp. strain TS-2, an ascomycete fungus isolated from a freshwater outflow and capable of Mn(II) oxidation Reviewed

    Tsushima, S, R. A. Kanaly, J. F. Mori

    Microbiology Resource Announcements   12   2023

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    Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    ABSTRACT

    Members of the genus Periconia are commonly found as plant-associated filamentous fungi. Here, the first draft genome sequence of a new Periconia strain, TS-2, that was isolated from freshwater outflow sediment and possesses the ability to oxidize dissolved Mn(II), was obtained and has an estimated size of 40.7 Mb.

    DOI: 10.1128/mra.00599-23

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  • Natural Chromosome-Chromid Fusion across rRNA Operons in a Burkholderiaceae Bacterium Reviewed

    Mori, J.F., R.A. Kanaly

    Microbiology Spectrum   10   2022

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    Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    A bacterial chromosome that was naturally fused with the secondary chromosome, or “chromid,” and presented as an unexpectedly large single replicon was discovered in the genome of
    <named-content content-type="genus-species">Cupriavidus necator</named-content>
    strain KK10, a biotechnologically useful member of the family
    <italic>Burkholderiaceae</italic>
    . Although
    <italic>Burkholderiaceae</italic>
    is a well-documented group that conserves chromids in their genomes, this chromosomal fusion event has not been previously reported for this family.

    DOI: 10.1128/spectrum.02225-21

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  • Growth of Sphingobium barthaii KK22 on 1-ethylnaphthalene reveals diverse oxidative transformations and a complex metabolite profile Reviewed

    Sakai, M, Y. Tomiyama, J.F. Mori, R.A. Kanaly

    International Biodeterioration & Biodegradation   175   2022

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ibiod.2022.105500

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  • Comprehensive Genomic Characterization of Marine Bacteria <i>Thalassospira</i> spp. Provides Insights into Their Ecological Roles in Aromatic Hydrocarbon-Exposed Environments Reviewed

    Kayama, G, R.A. Kanaly, J.F. Mori

    Microbiology Spectrum   10   2022

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    Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    Comprehensive investigation of multiple genomic data sets from targeted microbial taxa deposited in databases may provide substantial information to predict metabolic capabilities and ecological roles in different environments. This study is the first report that details the functional profiling of Thalassospira spp. that have repeatedly been found in polycyclic aromatic hydrocarbon (PAH)-exposed marine bacterial communities by using genomic data from a new isolate, Thalassospira strain GO-4, and other strains in databases.

    DOI: 10.1128/spectrum.03149-22

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  • Quantification of cyanobacterial cyclic di-guanosine monophosphate (c-di-GMP) by liquid chromatography electrospray ionization tandem mass spectrometry Reviewed

    Kameda, M, R.A. Kanaly, M. Harada, S. Aoki, H. Tsukada, S. Kutsuna

    Journal of Microbiological Methods   196   2022

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.mimet.2022.106468

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  • Complete Genome Sequence of <i>Thalassospira</i> sp. Strain GO-4, a Marine Bacterium Isolated from a Phenanthrene-Enriched Bacterial Consortium Reviewed

    Kayama, G., R.A. Kanaly, J.F. Mori

    Microbiology Resource Announcements   10   2022

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    Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    The genus Thalassospira has often been studied as a potential major contributing member of aromatic hydrocarbon-exposed microbial communities. Here, the complete genome sequence of a new isolate of Thalassospira , strain GO-4, was obtained and was confirmed to possess functional genes that are responsible for its metabolism of phthalic acid.

    DOI: 10.1128/mra.00532-22

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  • Multispecies diesel fuel biodegradation and niche formation are ignited by pioneer hydrocarbon-utilizing proteobacteria in a soil bacterial consortium Reviewed

    Mori, J. F, R. A. Kanaly

    Applied and Environmental Microbiology   87   2021

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  • Identification of a diguanylate cyclase that facilitates biofilm formation on electrodes by Shewanella oneidensis MR-1 Reviewed

    Matsumoto, A, R. Koga, R. A. Kanaly, A. Kouzuma, K. Watanabe

    Applied and Environmental Microbiology   87   2021

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    Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    In many bacteria, cyclic diguanosine monophosphate (c-di-GMP), synthesized by diguanylate cyclase (DGC), serves as a second messenger involved in the regulation of biofilm formation. Although studies have suggested that c-di-GMP also regulates the formation of electrochemically active biofilms (EABFs) by <italic>Shewanella oneidensis</italic> MR-1, DGCs involved in this process remained to be identified. Here we report that the SO_1646 gene, hereafter named <italic>dgcS</italic>, is upregulated under medium-flow conditions in electrochemical flow cells (EFCs), and its product (DgcS) functions as a major DGC in MR-1. <italic>In vitro</italic> assays demonstrated that purified DgcS catalyzed the synthesis of c-di-GMP from GTP. Comparisons of intracellular c-di-GMP levels in the wild-type strain and a <italic>dgcS</italic>-deletion mutant (Δ<italic>dgcS</italic>) showed that production of c-di-GMP was markedly reduced in Δ<italic>dgcS</italic> when cells were grown in batch cultures and on electrodes in EFCs. Cultivation of Δ<italic>dgcS</italic> in EFCs also revealed that the loss of DgcS resulted in impaired biofilm formation and decreased current generation. These findings demonstrate that MR-1 uses DgcS to synthesize c-di-GMP under medium-flow conditions, thereby activating biofilm formation on electrodes.


    <bold>IMPORTANCE</bold>


    Bioelectrochemical systems (BESs) have attracted wide attention owing to their utility in sustainable biotechnology processes, such as microbial fuel cells and electro-fermentation systems. In BESs, electrochemically active bacteria (EAB) form biofilms on electrode surfaces, thereby serving as effective catalysts for the interconversion between chemical and electric energy. It is therefore important to understand mechanisms for the formation of biofilm by EAB grown on electrodes. Here we show that a model EAB, <italic>S. oneidensis</italic> MR-1, expresses DgcS as a major DGC, thereby activating the formation of biofilms on electrodes via c-di-GMP-dependent signal transduction cascades. The findings presented herein provide the molecular basis for improving electrochemical interactions between EAB and electrodes in BESs. The results also offer molecular insights into how <italic>Shewanella</italic> regulates biofilm formation on solid surfaces in the natural environment.

    DOI: 10.1128/aem.00201-21

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  • Complete genome sequence of Cupriavidus necator KK10, an azaarene-degrading and polyhydroxyalkanoate-producing soil bacterium Reviewed

    Mori, J.F, M. Nagai, R.A. Kanaly

    Microbiology Resource Announcements   10   2021

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  • Complete genome sequence of Sphingobium barthaii KK22, a high-molecular-weight polycyclic aromatic hydrocarbon-degrading soil bacterium Reviewed

    Mori, J. F., R. A. Kanaly

    Microbiology Resource Announcements   10   2021

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  • Functionalization of the model asphaltene 1-dodecylnaphthalene by Pseudomonas aeruginosa KK6 through subterminal metabolism Reviewed

    Tomiyama, Y, T. Takeshita, J.F. Mori, R.A. Kanaly

    Journal of Petroleum Science and Engineering   205   108870 - 108870   2021

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    DOI: 10.1016/j.petrol.2021.108870

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  • Cometabolic benzo[a]pyrene biotransformation by Sphingobium barthaii KK22 proceeds through the kata-annelated ring and 1-pyrenecarboxylic acid to downstream products Reviewed

    Izawa, M, M. Sakai, J.F. Mori, R.A. Kanaly

    Journal of Hazardous Materials Advances   100018   2021

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    DOI: 10.1016/j.hazadv.2021.100018

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  • Chemical and genomic analyses of polycyclic aromatic hydrocarbon biodegradation in Sphingobium barthaii KK22 reveals divergent pathways in soil sphingomonads Reviewed

    Maeda, A.H, S. Nishi, Y. Hatada, Y. Ohta, K. Misaka, M. Kunihiro, J.F. Mori, R.A. Kanaly

    International Biodeterioration and Biodegradation   151   104993   2020

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    DOI: 10.1016/j.ibiod.2020.104993

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  • Characterization of N-(2,6-dimethylphenyl)hydroxylamine adducts of 2′-deoxyguanosine under weakly basic conditions Reviewed

    Matsui, T., N. Yamada, H. Kuno, R. A. Kanaly

    Chemosphere   252   2020

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  • In vitro DNA/RNA adductomics to confirm DNA damage caused by benzo[a]pyrene in the Hep G2 cell line Reviewed

    Takeshita, T., R. A. Kanaly

    Frontiers in Chemistry, Analytical Chemistry   7   491   2019

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  • Formation of bulky DNA adducts by non-enzymatic production of 1,2-naphthoquinone-epoxide from 1,2-naphthoquinone under physiological conditions Reviewed

    Matsui, T., N. Yamada, H. Kuno, R. A. Kanaly

    Chemical Research in Toxicology (ACS)   32   1760 - 1771   2019

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  • Triple quadrupole mass spectrometry comparative DNA adductomics of Hep G2 cells following exposure to safrole Reviewed

    Takeshita, T., F. Tao, N. Kojima, R. A. Kanaly

    Toxicology Letters   300   92 - 104   2019

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  • ヒト肝臓細胞株におけるDNAアダクトーム法を用いた反応性代謝物の推定

    竹下 俊英, 田尾 文哉, 小島 伸彦, Kanaly Robert

    生命科学系学会合同年次大会   2017年度   [2P - 1112]   2017.12

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    Language:Japanese   Publisher:生命科学系学会合同年次大会運営事務局  

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  • SUEL/RBL and their biomedical applications

    Yasuhiro Koide, Yuki Fujii, Imtiaj Hasan, Yukiko Ogawa, Sultana Rajia, Sarkar M.A. Kawsar, Robert Kanaly, Shigeki Sugawara, Masahiro Hosono, Jiharu Hamako, Taei Matsui, Yasuhiro Ozeki

    Marine OMICS: Principles and Applications   407 - 418   2016.11

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    Language:English   Publishing type:Part of collection (book)   Publisher:CRC Press  

    DOI: 10.1201/9781315372303

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  • RAMA casein zymography: Time-saving and highly sensitive casein zymography for MMP7 and trypsin Reviewed

    Hidetaro Yasumitsu, Yasuhiro Ozeki, Robert A. Kanaly

    Electrophoresis   37 ( 22 )   2959 - 2962   2016.11

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    DOI: 10.1002/elps.201600346

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  • Structural glycobiology for lectin to promote advanced biomedical research

    Imtiaj Hasan, Yuki Fujii, Sarkar M.A. Kawsar, Sultana Rajia, Shigeki Sugawara, Masahiro Hosono, Yukiko Ogawa, Yasushi Kawakami, Yasuhiro Koide, Daiki Yamamoto, Robert A. Kanaly, Yasuhiro Ozeki

    Marine Glycobiology: Principles and Applications   445 - 458   2016.10

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  • Analysis of the Fine-Tuning of Cyanobacterial Circadian Phase by Monochromatic Light and Long-Day Conditions Reviewed

    Takayuki Kobayashi, Yuji Obana, Naoyuki Kuboi, Yohko Kitayama, Shingo Hayashi, Masataka Oka, Naomichi Wada, Kyouhei Arita, Toshiyuki Shimizu, Mamoru Sato, Robert A. Kanaly, Shinsuke Kutsuna

    PLANT AND CELL PHYSIOLOGY   57 ( 1 )   105 - 114   2016.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcv177

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  • Application of DNA adductomics to soil bacterium Sphingobium sp strain KK22 Reviewed

    Robert A. Kanaly, Ruggero Micheletto, Tomonari Matsuda, Youko Utsuno, Yasuhiro Ozeki, Natsuko Hamamura

    MicrobiologyOpen   4 ( 5 )   841 - 856   2015.10

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    DOI: 10.1002/mbo3.283

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  • Aerobic biotransformation of 3-methylindole to ring cleavage products by Cupriavidus sp strain KK10 Reviewed

    Kimiko Fukuoka, Yasuhiro Ozeki, Robert A. Kanaly

    BIODEGRADATION   26 ( 5 )   359 - 373   2015.9

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    DOI: 10.1007/s10532-015-9739-0

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  • Sphingobium barthaii sp nova, a high molecular weight polycyclic aromatic hydrocarbon-degrading bacterium isolated from cattle pasture soil Reviewed

    Allyn H. Maeda, Marie Kunihiro, Yasuhiro Ozeki, Yuichi Nogi, Robert A. Kanaly

    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY   65   2919 - 2924   2015.9

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    DOI: 10.1099/ijs.0.000356

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  • Biotransformation of indole by Cupriavidus sp strain KK10 proceeds through N-heterocyclic- and carbocyclic-aromatic ring cleavage and production of indigoids Reviewed

    Kimiko Fukuoka, Keita Tanaka, Yasuhiro Ozeki, Robert A. Kanaly

    INTERNATIONAL BIODETERIORATION & BIODEGRADATION   97   13 - 24   2015.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ibiod.2014.11.007

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  • Biotransformation of the high-molecular weight polycyclic aromatic hydrocarbon ( PAH)benzo[ k] fluoranthene by Sphingobium sp strain KK22 and identification of new products of non-alternant PAH biodegradation by liquid chromatography electrospray ionization tandem mass spectrometry Reviewed

    Allyn H. Maeda, Shinro Nishi, Yuji Hatada, Yasuhiro Ozeki, Robert A. Kanaly

    MICROBIAL BIOTECHNOLOGY   7 ( 2 )   114 - 129   2014.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    A pathway for the biotransformation of the environmental pollutant and high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by a soil bacterium was constructed through analyses of results from liquid chromatography negative electrospray ionization tandem mass spectrometry (LC/ESI(-)-MS/MS). Exposure of Sphingobium sp. strain KK22 to benzo[k]fluoranthene resulted in transformation to four-, three- and two-aromatic ring products. The structurally similar four- and three-ring non-alternant PAHs fluoranthene and acenaphthylene were also biotransformed by strain KK22, and LC/ESI(-)-MS/MS analyses of these products confirmed the lower biotransformation pathway proposed for benzo[k]fluoranthene. In all, seven products from benzo[k]fluoranthene and seven products from fluoranthene were revealed and included previously unreported products from both PAHs. Benzo[k]fluoranthene biotransformation proceeded through ortho-cleavage of 8,9-dihydroxy-benzo[k]fluoranthene to 8-carboxyfluoranthenyl-9-propenic acid and 9-hydroxy-fluoranthene-8-carboxylic acid, and was followed by meta-cleavage to produce 3-(2-formylacenaphthylen-1-yl)-2-hydroxy-prop-2-enoic acid. The fluoranthene pathway converged with the benzo[k]fluoranthene pathway through detection of the three-ring product, 2-formylacenaphthylene-1-carboxylic acid. Production of key downstream metabolites, 1,8-naphthalic anhydride and 1-naphthoic acid from benzo[k]fluoranthene, fluoranthene and acenaphthylene biotransformations provided evidence for a common pathway by strain KK22 for all three PAHs through acenaphthoquinone. Quantitative analysis of benzo[k]fluoranthene biotransformation by strain KK22 confirmed biodegradation. This is the first pathway proposed for the biotransformation of benzo[k]fluoranthene by a bacterium.

    DOI: 10.1111/1751-7915.12102

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  • Biotransformation of the HMW PAH benzo[k]fluoranthene by Sphingobium sp. strain KK22 and identification of new products of non-alternant PAH biodegradation by LC/ESI-MS/MS analyses Reviewed International journal

    Maeda, A.H, S. Nishi, Y. Hatada, Y. Ozeki, R.A. Kanaly

    Microbial Biotechnology   7 ( 2 )   114 - 129   2014

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    A pathway for the biotransformation of the environmental pollutant and high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by a soil bacterium was constructed through analyses of results from liquid chromatography negative electrospray ionization tandem mass spectrometry (LC/ESI(-)-MS/MS). Exposure of Sphingobium sp. strain KK22 to benzo[k]fluoranthene resulted in transformation to four-, three- and two-aromatic ring products. The structurally similar four- and three-ring non-alternant PAHs fluoranthene and acenaphthylene were also biotransformed by strain KK22, and LC/ESI(-)-MS/MS analyses of these products confirmed the lower biotransformation pathway proposed for benzo[k]fluoranthene. In all, seven products from benzo[k]fluoranthene and seven products from fluoranthene were revealed and included previously unreported products from both PAHs. Benzo[k]fluoranthene biotransformation proceeded through ortho-cleavage of 8,9-dihydroxy-benzo[k]fluoranthene to 8-carboxyfluoranthenyl-9-propenic acid and 9-hydroxy-fluoranthene-8-carboxylic acid, and was followed by meta-cleavage to produce 3-(2-formylacenaphthylen-1-yl)-2-hydroxy-prop-2-enoic acid. The fluoranthene pathway converged with the benzo[k]fluoranthene pathway through detection of the three-ring product, 2-formylacenaphthylene-1-carboxylic acid. Production of key downstream metabolites, 1,8-naphthalic anhydride and 1-naphthoic acid from benzo[k]fluoranthene, fluoranthene and acenaphthylene biotransformations provided evidence for a common pathway by strain KK22 for all three PAHs through acenaphthoquinone. Quantitative analysis of benzo[k]fluoranthene biotransformation by strain KK22 confirmed biodegradation. This is the first pathway proposed for the biotransformation of benzo[k]fluoranthene by a bacterium.

    DOI: 10.1111/1751-7915.12102

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  • 9,10-Phenanthrenedione biodegradation by a soil bacterium and identification of transformation products by LC/ESI-MS/MS Reviewed

    Robert A. Kanaly, Natsuko Hamamura

    Chemosphere   92 ( 11 )   1442 - 1449   2013.9

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    DOI: 10.1016/j.chemosphere.2013.03.054

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  • Differential Arsenic Mobilization from As-Bearing Ferrihydrite by Iron-Respiring Shewanella Strains with Different Arsenic-Reducing Activities Reviewed

    Shenghua Jiang, Ji-Hoon Lee, Donghun Kim, Robert A. Kanaly, Min-Gyu Kim, Hor-Gil Hur

    ENVIRONMENTAL SCIENCE & TECHNOLOGY   47 ( 15 )   8616 - 8623   2013.8

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    DOI: 10.1021/es400534z

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  • Benz[a]anthracene Biotransformation and Production of Ring Fission Products by Sphingobium sp Strain KK22 Reviewed

    Marie Kunihiro, Yasuhiro Ozeki, Yuichi Nogi, Natsuko Hamamura, Robert A. Kanaly

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   79 ( 14 )   4410 - 4420   2013.7

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    DOI: 10.1128/AEM.01129-13

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  • Draft genome sequence of Sphingobium sp. strain KK22, a high-molecular-weight polycyclic aromatic hydrocarbon-degrading bacterium isolated from cattle pasture soil Reviewed

    Allyn H. Maeda, Shinro Nishi, Yasuhiro Ozeki, Yukari Ohta, Yuji Hatada, Robert A. Kanaly

    Genome Announcements   1 ( 6 )   1 - 2   2013

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    DOI: 10.1128/genomeA.00911-13

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  • Production of Natural Fragrance Aromatic Acids by Coexpression of trans-Anethole Oxygenase and p-Anisaldehyde Dehydrogenase Genes of Pseudomonas putida JYR-1 in Escherichia coli Reviewed

    Dongfei Han, Somwang Kurusarttra, Ji-Young Ryu, Robert A. Kanaly, Hor-Gil Hur

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   60 ( 48 )   11972 - 11979   2012.12

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  • Biological accumulation of tellurium nanorod structures via reduction of tellurite by Shewanella oneidensis MR-1 Reviewed

    Dong-Hun Kim, Robert A. Kanaly, Hor-Gil Hur

    BIORESOURCE TECHNOLOGY   125   127 - 131   2012.12

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    DOI: 10.1016/j.biortech.2012.08.129

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  • A Lectin from the Mussel Mytilus galloprovincialis Has a Highly Novel Primary Structure and Induces Glycan-mediated Cytotoxicity of Globotriaosylceramide-expressing Lymphoma Cells Reviewed

    Yuki Fujii, Naoshi Dohmae, Koji Takio, Sarkar M. A. Kawsar, Ryo Matsumoto, Imtiaj Hasan, Yasuhiro Koide, Robert A. Kanaly, Hidetaro Yasumitsu, Yukiko Ogawa, Shigeki Sugawara, Masahiro Hosono, Kazuo Nitta, Jiharu Hamako, Taei Matsui, Yasuhiro Ozeki

    JOURNAL OF BIOLOGICAL CHEMISTRY   287 ( 53 )   44772 - 44783   2012.12

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    DOI: 10.1074/jbc.M112.418012

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  • Isolation of a Gene Responsible for the Oxidation of trans-Anethole to para-Anisaldehyde by Pseudomonas putida JYR-1 and Its Expression in Escherichia coli Reviewed

    Dongfei Han, Ji-Young Ryu, Robert A. Kanaly, Hor-Gil Hur

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   78 ( 15 )   5238 - 5246   2012.8

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    DOI: 10.1128/AEM.00781-12

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  • Cytotoxicity and Glycan-Binding Properties of an 18 kDa Lectin Isolated from the Marine Sponge Halichondria okadai Reviewed

    Ryo Matsumoto, Yuki Fujii, Sarkar M. A. Kawsar, Robert A. Kanaly, Hidetaro Yasumitsu, Yasuhiro Koide, Imtiaj Hasan, Chihiro Iwahara, Yukiko Ogawa, Chang Hun Im, Shigeki Sugawara, Masahiro Hosono, Kazuo Nitta, Jiharu Hamako, Taei Matsui, Yasuhiro Ozeki

    TOXINS   4 ( 5 )   323 - 338   2012.5

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    DOI: 10.3390/toxins4050323

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  • MRP1 Expressed on Burkitt's Lymphoma Cells was Depleted by Catfish Egg Lectin Through Gb3-Glycosphingolipid and Enhanced Cytotoxic Effect of Drugs Reviewed

    Yuki Fujii, Shigeki Sugawara, Daisuke Araki, Tasuku Kawano, Takeo Tatsuta, Kohta Takahashi, Sarkar M. A. Kawsar, Ryo Matsumoto, Robert A. Kanaly, Hidetaro Yasumitsu, Yasuhiro Ozeki, Masahiro Hosono, Taeko Miyagi, Sen-itiroh Hakomori, Motoaki Takayanagi, Kazuo Nitta

    PROTEIN JOURNAL   31 ( 1 )   15 - 26   2012.1

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    DOI: 10.1007/s10930-011-9369-2

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  • Fine bubble mixing (FBM) culture of E. coli: a highly cost-effective middle scale-size culture system Reviewed

    Yasumitsu, H, H. Tajima, M. Isobe, S. Kutsuna, S. M. Kawsar, Y. Fujii, R. A. Kanaly, Y. Ozeki, E. Yokota

    Protein & Peptide Letters   20   213 - 217   2012

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  • Application of denaturing gradient gel electrophoresis as an ecotoxicological tool to investigate the effects of aqu-fullerene on a bacterial community Reviewed

    Kanaly, R. A, A. Maeda, M. Kunihiro, N. Hamamura

    Interdisciplinary Studies on Environmental Chemistry - Environmental Pollution and Ecotoxicology   6   79 - 88   2012

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  • Cytotoxicity and Glycan-Binding Profile of a d-Galactose-Binding Lectin from the Eggs of a Japanese Sea Hare (Aplysia kurodai) Reviewed

    Sarkar M. A. Kawsar, Ryo Matsumoto, Yuki Fujii, Haruki Matsuoka, Naoko Masuda, Iwahara Chihiro, Hidetaro Yasumitsu, Robert A. Kanaly, Shigeki Sugawara, Masahiro Hosono, Kazuo Nitta, Naoto Ishizaki, Chikaku Dogasaki, Jiharu Hamako, Taei Matsui, Yasuhiro Ozeki

    PROTEIN JOURNAL   30 ( 7 )   509 - 519   2011.10

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    DOI: 10.1007/s10930-011-9356-7

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  • Absolute configuration-dependent epoxide formation from isoflavan-4-ol stereoisomers by biphenyl dioxygenase of Pseudomonas pseudoalcaligenes strain KF707 Reviewed

    Jiyoung Seo, Su-Il Kang, Dongho Won, Mihyang Kim, Ji-Young Ryu, Suk-Woo Kang, Byung-Hun Um, Cheol-Ho Pan, Joong-Hoon Ahn, Youhoon Chong, Robert A. Kanaly, Jaehong Han, Hor-Gil Hur

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   89 ( 6 )   1773 - 1782   2011.3

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    DOI: 10.1007/s00253-010-2989-1

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  • CGP stain: An inexpensive, odorless, rapid, sensitive, and in principle in vitro methylation-free Coomassie Brilliant Blue stain Reviewed

    Hidetaro Yasumitsu, Yasuhiro Ozeki, Sarkar M. A. Kawsar, Tosifusa Toda, Robert Kanaly

    ANALYTICAL BIOCHEMISTRY   406 ( 1 )   86 - 88   2010.11

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    DOI: 10.1016/j.ab.2010.06.035

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  • High diversity and abundance of antibiotic-resistant Escherichia coli isolated from humans and farm animal hosts in Jeonnam Province, South Korea Reviewed

    Tatsuya Unno, Dukki Han, Jeonghwan Jang, Sun-Nim Lee, Joon Ha Kim, GwangPyo Ko, Bong Gyu Kim, Joong-Hoon Ahn, Robert A. Kanaly, Michael J. Sadowsky, Hor-Gil Hur

    SCIENCE OF THE TOTAL ENVIRONMENT   408 ( 17 )   3499 - 3506   2010.8

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    DOI: 10.1016/j.scitotenv.2010.04.046

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  • Location of flavone B-ring controls regioselectivity and stereoselectivity of naphthalene dioxygenase from Pseudomonas sp strain NCIB 9816-4 Reviewed

    Jiyoung Seo, Su-Il Kang, Ji-Young Ryu, Young-Ju Lee, Ki Deok Park, Mihyang Kim, Dongho Won, Hye-Yeon Park, Joong-Hoon Ahn, Youhoon Chong, Robert A. Kanaly, Jaehong Han, Hor-Gil Hur

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   86 ( 5 )   1451 - 1462   2010.5

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    DOI: 10.1007/s00253-009-2389-6

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  • Advances in the field of high-molecular-weight polycyclic aromatic hydrocarbon biodegradation by bacteria

    Robert A. Kanaly, Shigeaki Harayama

    MICROBIAL BIOTECHNOLOGY   3 ( 2 )   136 - 164   2010.3

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    DOI: 10.1111/j.1751-7915.2009.00130.x

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  • Time-dependent density functional theory-assisted absolute configuration determination of cis-dihydrodiol metabolite produced from isoflavone by biphenyl dioxygenase Reviewed

    Jiyoung Seo, Su-Il Kang, Mihyang Kim, Dongho Won, Haruko Takahashi, Joong-Hoon Ahn, Youhoon Chong, Eunjung Lee, Yoongho Lim, Robert A. Kanaly, Jaehong Han, Hor-Gil Hur

    ANALYTICAL BIOCHEMISTRY   397 ( 1 )   29 - 36   2010.2

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    DOI: 10.1016/j.ab.2009.10.020

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  • Shewanella-mediated synthesis of selenium nanowires and nanoribbons Reviewed

    Cuong Tu Ho, Jeong Won Kim, Won Bae Kim, Kyung Song, Robert A. Kanaly, Michael J. Sadowsky, Hor-Gil Hur

    JOURNAL OF MATERIALS CHEMISTRY   20 ( 28 )   5899 - 5905   2010

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    DOI: 10.1039/b923252d

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  • Rapid oxidation of ring methyl groups is the primary mechanism of biotransformation of gemfibrozil by the fungus Cunninghamella elegans Reviewed

    Su-Il Kang, Seo-Young Kang, Robert A. Kanaly, Eunjung Lee, Yoongho Lim, Hor-Gil Hur

    ARCHIVES OF MICROBIOLOGY   191 ( 6 )   509 - 517   2009.6

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    DOI: 10.1007/s00203-009-0480-0

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  • Report of the UNESCO conference on ethics of energy technologies: energy flow, environment and ethical implications for meat production

    Kanaly, R. A, D. Macer

    Eubios Journal of Asian and International Bioethics   18   143 - 148   2008

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  • Application of the adductome approach to assess intertissue DNA damage variations in human lung and esophagus Reviewed

    Robert A. Kanaly, Aburo Matsui, Tomoyuki Hanaoka, Tomonari Matsuda

    MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS   625 ( 1-2 )   83 - 93   2007.12

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    DOI: 10.1016/j.mrfmmm.2007.05.006

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  • Increased formation of hepatic N-2-ethylidene-2'-deoxyguanosine DNA adducts in aldehyde dehydrogenase 2-knockout mice treated with ethanol Reviewed

    Tomonari Matsuda, Akiko Matsumoto, Mitsuhiro Uchida, Robert A. Kanaly, Kentaro Misaki, Shinya Shibutani, Toshihiro Kawamoto, Kyoko Kitagawa, Keiichi I. Nakayama, Katsumaro Tomokuni, Masayoshi Ichiba

    CARCINOGENESIS   28 ( 11 )   2363 - 2366   2007.11

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    DOI: 10.1093/carcin/bgm057

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  • Metabolic characterization of newly isolated Pseudomonas nitroreducens Jin1 growing on eugenol and isoeugenol Reviewed

    Tatsuya Unno, Soo-Jin Kim, Robert A. Kanaly, Joong-Hoon Ahn, Su-Il Kang, Hor-Gil Hur

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   55 ( 21 )   8556 - 8561   2007.10

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    DOI: 10.1021/jf0715929

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  • Bioremediation technologies for treatment of PAH-contaminated soil and strategies to enhance process efficiency Reviewed

    S. Venkata Mohan, Takuro Kisa, Takeru Ohkuma, Robert A. Kanaly, Yoshihisa Shimizu

    Reviews in Environmental Science and Biotechnology   5 ( 4 )   347 - 374   2006.11

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    DOI: 10.1007/s11157-006-0004-1

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  • Increased DNA damage in ALDH2-deficient alcoholics Reviewed

    Tomonari Matsuda, Hisatoshi Yabushita, Robert A. Kanaly, Shinya Shibutani, Akira Yokoyama

    CHEMICAL RESEARCH IN TOXICOLOGY   19 ( 10 )   1374 - 1378   2006.10

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    DOI: 10.1021/tx060113h

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  • Development of the adductome approach to detect DNA damage in humans Reviewed

    Robert A. Kanaly, Tomoyuki Hanaoka, Haruhiko Sugimura, Hirokazu Toda, Saburo Matsui, Tomonari Matsuda

    ANTIOXIDANTS & REDOX SIGNALING   8 ( 5-6 )   993 - 1001   2006.5

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    DOI: 10.1089/ars.2006.8.993

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  • Growth of Phanerochaete chrysosporium on diesel fuel hydrocarbons at neutral pH Reviewed

    RA Kanaly, HG Hur

    CHEMOSPHERE   63 ( 2 )   202 - 211   2006.4

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    DOI: 10.1016/j.chemosphere.2005.08.022

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  • Bioavailability of benzo[a]pyrene during NAPL-CD enhanced biodegradation in soil and in liquid culture. Reviewed

    R. A. Kanaly, K. Watanabe, S. Matsui

    WATER SCIENCE AND TECHNOLOGY   53 ( 11 )   17 - 25   2006

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    DOI: 10.2166/wst.2006.333

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  • Biotransformation of 3-methyl-4-nitrophenol, a main product of the insecticide fenitrothion, by Aspergillus niger Reviewed

    RA Kanaly, IS Kim, HG Hur

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   53 ( 16 )   6426 - 6431   2005.8

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    DOI: 10.1021/jf050679w

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  • Multiple mechanisms contribute to the biodegradation of benzo[a]pyrene by petroleum-derived multicomponent nonaqueous-phase liquids Reviewed

    RA Kanaly, K Watanabe

    ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY   23 ( 4 )   850 - 856   2004.4

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    DOI: 10.1897/03-191

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  • Rhodanobacter sp strain BPC1 in a benzo[a]pyrene-mineralizing bacterial consortium Reviewed

    RA Kanaly, S Harayama, K Watanabe

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   68 ( 12 )   5826 - 5833   2002.12

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    DOI: 10.1128/AEM.68.12.5826-5833.2002

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  • Enhanced mineralization of benzo[a]pyrene in the presence of nonaqueous phase liquids Reviewed

    RA Kanaly, R Bartha, K Watanabe, S Harayama

    ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY   20 ( 3 )   498 - 501   2001.3

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    DOI: 10.1897/1551-5028(2001)020<0498:EMOBAP>2.0.CO;2

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  • Rapid mineralization of benzo[a]pyrene by a microbial consortium growing on diesel fuel Reviewed

    RA Kanaly, R Bartha, K Watanabe, S Harayama

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   66 ( 10 )   4205 - 4211   2000.10

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    DOI: 10.1128/AEM.66.10.4205-4211.2000

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  • Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by bacteria Reviewed

    RA Kanaly, S Harayama

    JOURNAL OF BACTERIOLOGY   182 ( 8 )   2059 - 2067   2000.4

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    DOI: 10.1128/JB.182.8.2059-2067.2000

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  • Cometabolic mineralization of benzo[a]pyrene caused by hydrocarbon additions to soil Reviewed

    RA Kanaly, R Bartha

    ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY   18 ( 10 )   2186 - 2190   1999.10

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    DOI: 10.1897/1551-5028(1999)018<2186:CMOBAP>2.3.CO;2

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  • Biodegradation of [C-14]benzo[a]pyrene added in crude oil to uncontaminated soil Reviewed

    R Kanaly, R Bartha, S Fogel, M Findlay

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   63 ( 11 )   4511 - 4515   1997.11

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Books

  • Ability of Diverse Marine Invertebrate Lectins to Regulate Cell Function

    Ozeki, Y, S.M. Kawsar, Y. Fujii, Y. Ogawa, S. Sugawara, I. Hasan, Y. Koide, H. Yasumitsu, R.A. Kanaly( Role: Joint authorIn S.-K. Kwon (Ed.), Marine Proteins and Peptides: Biological Activities and Applications)

    Wiley-Blackwell, England  2013 

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  • Negative Externalities of Intensive Meat Production

    Kanaly Robert A( Role: Joint authorIn R. Moorthy and D. Macer (Eds.), Ethics and Politics of Resources for Human Security and Well-being)

    UNESCO, Paris  2013 

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  • Energy Flow, Environment and Ethical Implications for Meat Production

    Kanaly, R. A, L. I. O. Manzanero, G. Foley, S. Panneerselvam, D. Macer( Role: Sole authorvi + 55 pp.)

    UNESCO Bangkok.  2010  ( ISBN:9789292233471, 9789292233488

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  • DNA Adductomics: Global Assessment of DNA Damage

    Kanaly R. A, T. Matsuda( Role: Joint authorIn H. Kimura and A. Suzuki (Eds.), New Research on DNA Damage)

    Nova Science Publishers  2009  ( ISBN:9781604565812

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Presentations

  • Biodegradation of aliphatic hydrocarbons and heavy fuel oil by Sphingobium barthaii KK22

    Sakai, M., Mori, J. F., Kanaly, R.A.

    The 2025 Annual Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry (JSBBA 2025), Mar. 3-8, 2025, Sapporo, Hokkaido, Japan. 

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  • Insights into the biodegradation mechanism of environmental pollutants in Pseudomonas migulae under cold stress

    Yanagita, H., R. A. Kanaly, J. F. Mori

    The 37th Japanese Symposium on Microbial Ecology (JSME 2024), Oct. 28-31, Hiroshima, Japan 

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  • Biodegradation of the C9 aromatic pollutant n-propylbenzene by Sphingobium barthaii KK22

    Sakai, M., J. F. Mori, R. A. Kanaly

    The 37th Japanese Symposium on Microbial Ecology (JSME 2024), Oct. 28-31, Hiroshima, Japan 

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  • Identification of a putative novel polycyclic aromatic hydrocarbon-biodegrading gene cluster shared among marine Roseobacteraceae

    Mori, J. F., M. Abe, R. A. Kanaly

    The 37th Japanese Symposium on Microbial Ecology (JSME 2024), Oct. 28-31, Hiroshima, Japan 

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    Event date: 2024.10

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  • Biodegradation of hydrocarbons in atmospheric distillation column residue oil by single strains of bacteria.

    Sakai, M, Mori, J. F, Kanaly, R.A

    The 2024 Annual Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry (JSBBA 2024), Mar. 24-27, 2024, Tokyo, Japan. 

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  • Function of the second messenger cyclic di-GMP in Synechococcus elongatus PCC 7942

    Yamaguchi, C, R. A. Kanaly, E. Nishizaki, K. Yamashita, K. Satsuki, M. Harada, M. Hirai, M. Tsukamoto, S. Aoki, Y. Nakahira, Y. Furuike, S. Akiyama, M. Fang, S. Golden, S. Kutsuna

    The 65th Meeting of the Japanese Society of Plant Physiologists, Mar. 17-19, 2024, Kobe, Japan. 

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  • Application of LC-ESI-HRMS metabolomics to study the cooperative bacterial biotransformation of a model asphaltene

    Sakai, M, Mori, J. F, Kanaly, R.A

    The 36th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2023) and the 13th Annual Asian Symposium on Microbial Ecology (ASME), Nov. 27-30, 2023, Hamamatsu, Shizuoka, Japan 

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  • Inconspicuous but indispensable: Phenanthrene biodegradation by Sagittula that represented a minor population in a phenanthrene enriched marine bacterial consortium

    Mori, J. F, Abe, M, Kanaly, R.A, Mori, J. F

    The 36th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2023) and the 13th Annual Asian Symposium on Microbial Ecology (ASME), Nov. 27-30, 2023, Hamamatsu, Shizuoka, Japan 

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  • Discovery of a novel PAH biodegradation gene cluster on a plasmid in the marine bacterium Sagittula sp.

    Abe, M, Kayama, G, Kanaly, R.A, Mori, J. F

    The 36th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2023) and the 13th Annual Asian Symposium on Microbial Ecology (ASME), Nov. 27-30, 2023, Hamamatsu, Shizuoka, Japan 

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  • PHA production from waste biodiesel glycerol and levulinic acid by a novel methanol-tolerant bacterial strain Cupriavidus necator KK10

    Nagai, M, Mori, J. F, Kanaly, R.A

    The 36th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2023) and the 13th Annual Asian Symposium on Microbial Ecology (ASME), Nov. 27-30, 2023, Hamamatsu, Shizuoka, Japan 

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  • Biodegradation of the polyalkyl chain-alkylated polycyclic aromatic hydrocarbon (CA-PAH), 1-butylnaphthalene by a soil bacterium

    Sakai, M, Mori, J. F, Kanaly, R.A

    The Annual Meeting of the Japan Society for Biotechnology (JSEB 2023), June 8 - 9, 2023, Okayama, Japan 

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  • Pioneer and follower bacteria in a petroleum biodegradation microcosm

    Mori, J. F, Sakai, M, Kanaly, R. A

    The 35th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2022), Oct. 31 – Nov. 3, 2022, Sapporo, Hokkaido, Japan 

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  • Biotransformation of short and long polyalkyl chain-alkylated polycyclic aromatic hydrocarbons by soil bacteria

    Sakai, M, Mori, J. F, Kanaly, R. A

    The 35th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2022), Oct. 31 – Nov. 3, 2022, Sapporo, Hokkaido, Japan 

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  • Production of polyhydroxyalkanoates (PHAs) from biodiesel waste glycerol and levulinic acid by soil bacterium Cupriavidus necator KK10

    Nagai, M, J.F. Mori, R.A. Kanaly

    The 35th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2022), Oct. 31 – Nov. 3, 2022, Sapporo, Hokkaido, Japan 

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  • Isolation of a novel freshwater manganese-oxidizing fungus Periconia and evaluation of its water purification potential

    Tsushima, S, Nishi, Y, Suzuki, R, Tachibana, M, Kanaly, R. A, Mori, J. F

    The 35th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2022), Oct. 31 – Nov. 3, 2022, Sapporo, Hokkaido, Japan 

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  • Search for oil-degrading pioneer bacteria modeled on a coastal seawater microbial community

    Kayama, G, R.A. Kanaly, J.F. Mori

    The 34th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2021), Oct. 30 – Nov. 2, 2021, online. 

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  • Oxidative transformation of alkylated polycyclic aromatic hydrocarbons by a soil bacterium

    Sakai, M, Y. Tomiyama, J. F. Mori, R. A. Kanaly

    The 34th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2021), Oct. 30 – Nov. 2, 2021, online. 

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  • Investigation of polyhydroxyalkanoate (PHA) production by Cupriavidus necator strain KK10 when grown on glycerol and waste glycerol from biodiesel production

    Nagai, M, J. F. Mori, R. A. Kanaly

    The 34th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2021), Oct. 30 – Nov. 2, 2021, online. 

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  • Investigation of an adduct formation mechanism for N-oxidized aniline derivatives with 2’-deoxyguanosine

    Matsui, T, N. Yamada, H. Kuno, R.A. Kanaly

    The 37th Medicinal Chemistry Symposium (MCS2019), Nov. 27 - 29, 2019, Hachioji, Tokyo, Japan 

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  • Examination of the micronucleus test in T24 human bladder carcinoma cells

    Yamazeki, Y, R. Kanauchi, A. Nagano, R.A. Kanaly, T. Takeshita

    Joint Meeting of the 6th Asian Congress on Environmental Mutagens (ACEM) and the 48th Annual Meeting of the Japanese Environmental Mutagen Society (JEMS 2022), Nov. 18 - 20, 2019, Chiyoda, Tokyo, Japan 

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  • Verification of DNA adduct formation derived from chemical compounds by DNA/RNA adductome methods and in silico: ab initio calculation analyses

    Takeshita, T, H. Sakagami, M. Tachikawa, R.A. Kanaly

    Joint Meeting of the 6th Asian Congress on Environmental Mutagens (ACEM) and the 48th Annual Meeting of the Japanese Environmental Mutagen Society (JEMS 2022), Nov. 18 - 20, 2019, Chiyoda, Tokyo, Japan 

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  • SO_1646 encodes a diguanylate cyclase that determines intracellular cyclic di-GMP levels and regulates biofilm formation and current generation by Shewenella oneidensis MR-1

    Matsumoto, A, R. Koga, R.A. Kanaly, A. Kouzuma, K. Watanabe

    International Society for Microbial Electrochemistry and Technology 7 (ISMET), Oct. 7 - 14, 2019, Okinawa, Japan 

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    Event date: 2019.10

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  • The oil-eating gangs: metagenomics revealed the multi-species oil hydrocarbon-degrading soil bacterial consortium evolved in the lab-scale enrichment.

    Mori, J.F, Y. Tomiyama, R.A. Kanaly

    The 33rd Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2019), Sept. 10 - 13, 2019, Yamanashi, Japan 

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    Event date: 2019.9

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  • Investigation of a hydrocarbon pollutant-degrading bacterial consortium and Bradyrhizobium sp. strain KK5

    Tomiyama, Y, J.F. Mori, R.A. Kanaly

    The 33rd Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2019), Sept. 10 - 13, 2019, Yamanashi, Japan 

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    Event date: 2019.9

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  • RNA transcriptomics and DNA adduct analyses to investigate the effects of 1,2-naphthoquinone on bacterial cells

    Takeshita, T, Takaki, Y, Murayama, K, R.A. Kanaly

    47th Annual Meeting of the Japanese Environmental Mutagen Society (JEMS 2018), Nov. 1 -2, 2018, Kyoto, Japan 

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    Event date: 2018.11

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  • Application of the DNA/RNA adductome method for nucleic acid injury investigation of thiazolidinedione compounds

    Takeshita, T, R.A. Kanaly

    47th Annual Meeting of the Japanese Environmental Mutagen Society (JEMS 2018), Nov. 1 -2, 2018, Kyoto, Japan 

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    Event date: 2018.11

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  • Comparative non-targeted DNA adductomics of Hep G2 cells exposed to substituted monaromatic compounds

    Takeshita, T, R.A. Kanaly

    Society of Environmental Toxicology and Chemistry (SETAC) Asia-Pacific 2018, Sept. 16 - 19, 2018, Daegu, South Korea 

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    Event date: 2018.9

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  • Comparative investigation of DNA and RNA adductome methods to detect nucleic acid-reactive metabolites

    Takeshita, T, R.A. Kanaly

    The 45th Annual Meeting of the Japanese Society of Toxicology (JSOT), July 18 - 20, 2018, Osaka, Japan 

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    Event date: 2018.7

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  • Investigation of reactive metabolites in human liver cell line by use of the DNA adductome method International conference

    Takeshita, T, F. Tao, N. Kojima, R.A. Kanaly

    Consortium of Biological Sciences 2017 (ConBio2017), Dec. 6 - 9, 2017, Kobe, Japan 

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    Event date: 2017.12

    Language:Japanese  

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  • Comprehensive analysis of DNA adducts in human liver cells after exposure to safrole

    Takeshita, T, F. Tao, N. Kojima, R.A. Kanaly

    The 23st General Meeting of the Japanese Society of Food Chemistry, June 1 - 2, 2017, Ise City, Mie, Japan 

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    Event date: 2017.6

    Venue:Mie, Japan  

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  • Biodegradation of high concentrations of model fuel hydrocarbon NAPLs by a bacterium isolated from soil

    Takeshita, T, R. A. Kanaly

    The 31st Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2016), Oct. 22 - 25, 2016, Yokosuka, Japan 

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    Event date: 2016.10

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  • Chemical and genetic analyses of polycyclic aromatic hydrocarbon biodegradation by Sphingobium barthaii KK22 International conference

    Maeda, A.H, S. Nishi, Y. Hatada, Y. Ohta, Y. Ozeki, M. Kunihiro, R.A. Kanaly

    The 31st Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2016), Oct. 22 - 25, 2016, Yokosuka, Japan 

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    Event date: 2016.10

    Language:Japanese  

    Venue:Yokosuka, Japan  

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  • Biodegradation of benzo[a]pyrene by Sphingobium barthaii KK22 and effects on co-occurring PAH biodegradation

    Izawa, M, R.A. Kanaly

    The 31st Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2016), Oct. 22 - 25, 2016, Yokosuka, Japan 

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    Event date: 2016.10

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  • Bacterial biodegradation of sulfur-containing aromatic pollutants and the production of disulfides measured by LC-ESI-MS2

    Ozawa, K, Nemoto, Y, Kikuchi, M, R. A. Kanaly

    The 31st Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2016), Oct. 22 - 25, 2016, Yokosuka, Japan 

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    Event date: 2016.10

    Language:Japanese  

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  • Biotransformation of PAHs by Sphingobium barthaii and investigation of DNA damage by LC/ESI-MS/MS

    Fukuoka, K, R.A. Kanaly

    ASM Microbe 2016, June 16 - 20, 2016, Boston, MA, USA 

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    Event date: 2016.6

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  • Investigation of DNA adducts in PAH-degrading bacterial cells after exposure to hydrogen peroxide by DNA adductomics

    Fukuoka, K, R.A. Kanaly

    The 44th Annual Meeting of the Japanese Environmental Mutagen Society (JEMS 2015), Nov. 27 - 28, 2015, Fukuoka, Japan 

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    Event date: 2015.11

    Language:Japanese  

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  • Analysis of DNA damage in bacterial cells by DNA adductomics International conference

    Kanaly, R A, K. Fukuoka, R. Micheletto

    7th International Congress of the Asian Society of Toxicology (ASIATOX 2015), June 23 - 26, 2015, Jeju, South Korea 

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    Event date: 2015.6

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  • LC/ESI-MS/MS determination of products from the biotransformation of benzo[k]fluoranthene and non-alternant PAHs by a soil bacterium International conference

    Maeda, A.H, S. Nishi, Y. Ozeki, Y. Hatada, R.A. Kanaly

    The Twelfth Asian Conference on Analytical Sciences (ASIANALYSIS XII), Aug. 22 - 24, 2013, Fukuoka, Kyushu, Japan 

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    Event date: 2013.8

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  • Software-aided processing of DNA adductomics data obtained by liquid chromatography electrospray ionization tandem mass spectrometry International conference

    Kanaly, R A, A. H. Maeda, N. Hamamura, R. Micheletto

    The 13th International Congress of Toxicology (ICT XIII); The 2nd International Conference on Environmental Omics, June 30 - July 4, 2013, COEX Seoul, South Korea 

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    Event date: 2013.6 - 2013.7

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  • Investigation of DNA modifications in a soil bacterium after exposure to organic and inorganic pollutants by LC-tandem mass spectrometry International conference

    Kanaly, R A, R. Micheletto, A. H. Maeda, N. Hamamura

    113th General Meeting, American Society for Microbiology (ASM 2013), May 18 - 20, 2013, Denver, CO, USA 

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    Event date: 2013.5

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  • LC/ESI(-)-MS/MS analyses of the biotransformation products of dibenzo-p-dioxin by Sphingobium sp. strain KK22 International conference

    Maeda, A. H, E. Aoki, R. A. Kanaly

    19th International Mass Spectrometry Conference (IMSC2012), Sept. 15-21, 2012, Kyoto, Japan 

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    Event date: 2012.9

    Language:English  

    Venue:Kyoto, Japan  

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  • Investigation of a microbial community exposed to potentially genotoxic pollutants during growth on a defined hydrocarbon NAPL International conference

    Kanaly, R A, N. Hamamura

    112th General Meeting, American Society for Microbiology (ASM 2012), June 16 - 19, 2012, San Francisco, CA, USA 

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    Event date: 2012.6

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  • LC/ESI-MS/MS analyses of the biotransformation products of the sulfur heterocycle benzothiophene shows initiation on both the 5- and 6-member rings and disulfide production by Sphingobium sp. strain KK22 International conference

    Nemoto, Y, R. A. Kanaly

    International Union of Microbiological Societies 2011 Congress (IUMS 2011), Sept. 6 - 10, 2011, Sapporo, Hokkaido, Japan 

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    Event date: 2011.9

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  • Biotransformation of the PAHs fluorene and anthracene by Sphingobium sp. strain KK22 proceeds via meta-cleavage pathways as determined by LC/ESI-MS/MS International conference

    Suehiro, N, M. Kunihiro, R. A. Kanaly

    International Union of Microbiological Societies 2011 Congress (IUMS 2011), Sept. 6 - 10, 2011, Sapporo, Hokkaido, Japan 

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    Event date: 2011.9

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  • Effects of potential oxidative damage on bacterial populations caused by fullerene exposure International conference

    Kanaly, R A, M. Kunihiro, N. Hamamura

    International Union of Microbiological Societies 2011 Congress (IUMS 2011), Sept. 6 - 10, 2011, Sapporo, Hokkaido, Japan 

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    Event date: 2011.9

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  • Salicylic acid stimulates the cometabolic biotransformation of the 3-ring PAH fluorene by a phenanthrene-degrading bacterium International conference

    Suehiro, N, A. Katori, R. A. Kanaly

    The 26th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2010), Nov. 24 - 26, 2010, Tsukuba University, Tsukuba, Japan 

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    Event date: 2010.11

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  • Liquid chromatography tandem mass spectrometry analyses of the bacterial biotransformation products of the PAH sulfur heterocycle benzothiophene International conference

    Nemoto, Y, R. A. Kanaly

    The 26th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2010), Nov. 24 - 26, 2010, Tsukuba University, Tsukuba, Japan 

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    Event date: 2010.11

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  • Biodegradation of kata-annelated PAHs by Sphingobium species strain KK22 International conference

    Kunihiro, M, N. Suehiro, R. A. Kanaly

    The 26th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2010), Nov. 24 - 26, 2010, Tsukuba University, Tsukuba, Japan 

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    Event date: 2010.11

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  • Bacterial biotransformation of the PAH nitrogen heterocycle, 9N-carbazole and metabolite analyses by liquid chromatography tandem mass spectrometry International conference

    Mizumura, S, S. Uno, R. A. Kanaly

    The 26th Annual Meeting of the Japanese Society of Microbial Ecology (JSME 2010), Nov. 24 - 26, 2010, Tsukuba University, Tsukuba, Japan 

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    Event date: 2010.11

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  • LC/ESI-MS/MS real-time monitoring of the biodegradation of the environmental pollutant dibenzofuran by a newly-isolated bacterium International conference

    Arai, T, R. A. Kanaly

    The 58th Annual Conference on Mass Spectrometry and The 1st Asian and Oceanic Mass Spectrometry Conference, June 15 - 18, 2010, Tsukuba International Congress Center, Tsukuba, Japan 

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    Event date: 2010.6

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  • Biodegradation of benz[a]anthracene by Sphingobium species strain KK22 International conference

    Kunihiro, M, R. A. Kanaly

    110th General Meeting, American Society for Microbiology (ASM 2010), May 23 - 27, 2010, San Diego, CA, USA 

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    Event date: 2010.5

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  • Biodegradation and growth of a new Sphingobium sp. isolate on the PAH phenanthrene International conference

    Kunihiro, M, R. A. Kanaly

    Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA 2010), March 27 - 30, 2010, Tokyo University, Tokyo, Japan 

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    Event date: 2010.3

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  • The negative externalities of meat production Invited International conference

    Kanaly, R. A, D. Macer, L. I. O. Manzanero, S. Panneerselvam

    Joint UNESCO-UKM (Universiti Kebangsaan Malaysia) Conference on Politics of Resources, Human Security and Wellbeing, June, 2009, Kuala Lumpur, Malaysia 

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    Event date: 2009.6

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  • Energy flow, environment and ethical implications for meat production Invited International conference

    Kanaly, R. A, D. Macer, L. I. O. Manzanero, S. Panneerselvam

    The 6th Ordinary Session of the World Commission on the Ethics of Scientific Knowledge and Technology (COMEST UNESCO), June, 2009, Kuala Lumpur, Malaysia 

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    Event date: 2009.6

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  • Microbial consortium BPC enhances polycyclic aromatic hydrocarbon biodegradation in a coal and oil waste-site soil International conference

    Kanaly, R. A, K. Watanabe

    109th General Meeting, American Society for Microbiology (ASM 2009), May 17 - 21, Philadelphia, PA, USA 

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    Event date: 2009.5

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  • Detection and evaluation of DNA adducts in human pancreas and spleen by the DNA adductomics methodology International conference

    Kanaly, R. A, P. Chou, H. Nagayoshi, H. Sugimura, T. Matsuda

    The 37th Annual Meeting of the Japanese Environmental Mutagen Society (JEMS 2008), Dec. 4 - 6, 2008, Ginowan, Okinawa, Japan 

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    Event date: 2008.12

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  • DNA adductomics: global survey of DNA damage in human tissues Invited International conference

    Matsuda, T, R. A. Kanaly, P. Chou

    The 38th Annual Meeting of the European Environmental Mutagen Society (EEMS 2008), Sept. 21 - 25, 2008, Cavtat, Croatia 

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    Event date: 2008.9

    Language:English  

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  • Utilization of DNA adductomics to assess DNA damage in human lung, liver, kidney and large intestine International conference

    Kanaly, R. A, P. Chou, H. Nagayoshi, H. Sugimura, T. Matsuda

    The 38th Annual Meeting of the European Environmental Mutagen Society (EEMS 2008), Sept. 21 - 25, 2008, Cavtat, Croatia 

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    Event date: 2008.9

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  • Comparison of cumulative DNA damage in human organs by the newly developing adductome approach International conference

    Nagayoshi, H, R. A. Kanaly, T. Matsuda

    The 4th Joint Meeting of the Society For Free Radical Research Australasia and Japan, Dec. 1 - 5, 2007, Kyoto, Japan 

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    Event date: 2007.12

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  • Utilization of the adductome approach to assess DNA damage in human liver tissue International conference

    Kanaly, R. A, H. Nagayoshi, T. Hanaoka, T. Matsuda

    36th Annual Meeting of the Japanese Mutagen Society (JEMS 2007), Nov. 29 - 30, 2007, Kitakyushu, Kyushu, Japan 

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    Event date: 2007.11

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  • Extensive mineralization of benzo[a]pyrene in soil, supported by crude oil components

    Kanaly, R. A, S. Fogel, M. Findlay, R. Bartha

    97th General Meeting, American Society for Microbiology  1997.5 

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  • Biodegradation of diesel fuel hydrocarbons by Phanerochaete chrysosporium International conference

    Kanaly R. A, H.-G. Hur

    Proceedings of The Tenth International Symposium on Microbial Ecology (ISME 10)  2004.8 

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  • Analysis of a benzo[a]pyrene-mineralizing consortium by radiorespirometry and molecular methods International conference

    Kanaly R. A, R. Bartha, K. Watanabe, S. Harayama

    Annual Meeting of The Japan Society for Bioscience and Bioengineering  1999.9 

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  • Characterization of a rapid benzo[a]pyrene-mineralizing consortium enriched from soil International conference

    Kanaly, R. A, R. F. Sullivan, R. Bartha

    Proceedings of The Eighth International Symposium on Microbial Ecology (ISME 8)  1998.8 

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  • Benzo[a]pyrene biodegradation by a mixed bacterial community International conference

    Kanaly, R. A, K. Watanabe, S. Harayama

    Proceedings of The Fifth International Symposium on Environmental Biotechnology (ISEB 2000)  2000.7 

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  • Investigation of the community structure and cometabolic nature of a bacterial consortium which rapidly mineralizes benzo[a]pyrene International conference

    Kanaly, R. A, K. Watanabe, S. Harayama

    100th General Meeting, American Society for Microbiology  2000.5 

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  • Factors stimulating biodegradation of benzo[a]pyrene by a bacterial consortium International conference

    Kanaly R. A, K. Watanabe, S. Harayama

    First European Bioremediation Conference  2001.7 

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  • Benzo[a]pyrene mineralization in nonaqueous phase liquids International conference

    Kanaly R. A, K. Watanabe, S. Harayama

    Annual Meeting of The Japan Society for Bioscience and Bioengineering  2000.8 

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  • Fungal transformation of 3-methyl-4-nitrophenol, a product of Fenitrothion pesticide degradation in the environment International conference

    Kanaly R. A, I. S. Kim, H. G. Hur

    104th General Meeting, American Society for Microbiology,  2004.5 

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  • Physical and biological effects on NAPL-mediated polycyclic aromatic hydrocarbon biodegradation International conference

    Kanaly R. A, S. Harayama, K. Watanabe

    103rd General Meeting, American Society for Microbiology  2003.5 

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  • Rapid benzo[a]pyrene mineralization induced by cosubstrates in soil and in liquid culture International conference

    Kanaly, R. A, R. Bartha

    98th General Meeting, American Society for Microbiology  1998.5 

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  • Removal of the organochlorine insecticide Methoxychlor from solution by Trichoderma viride

    Kanaly R. A, H.-G. Hur

    International Water Association (IWA) Specialty Conference on Wastewater Reclamation and Reuse for Sustainability (WRRS 2005)  2005.11 

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  • Development of the adductome approach to detect endogenous DNA damage International conference

    Kanaly, R. A, Matsuda, T

    The 35th Annual Meeting of the Japanese Environmental Mutagen Society  2006.11 

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  • Development of Methods for the Detection of Multiple Forms of DNA Damage in Human Tissue Invited International conference

    Kanaly, R.A, Matsuda, T

    Fourth IERC Workshop on Environment and Sustainable Development, United Nations University-GIST  2006.10 

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  • Energy production and meat production: Ethical considerations Invited International conference

    Kanaly, R. A

    UNESCO/Thai Ministry of Science and Technology, Ethics of Energy Technologies in Asia and the Pacific  2007.9 

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  • Radiorespirometry as a tool for elucidating benzo[a]pyrene biodegradation patterns by a bacterial consortium. International conference

    Kanaly, R. A, K. Watanabe

    107th General Meeting, American Society for Microbiology  2007.5 

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  • Global Environmental Health Impacts of Industrialized Meat Production Invited International conference

    Kanaly, R.A

    2007 Korean Society for Economics and Environmental Geology International Symposium on Applied Geochemistry  2007.11 

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  • Mapping and identification of DNA damage in human organs by the adductome approach International conference

    Kanaly, R. A, T. Hanaoka, T. Matsuda

    Genomes, Medicine and the Environment 2007 (GME2007)  2007.10 

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  • DNA adductome strategy for the detection of multiple DNA adducts

    Matsuda, T, R. A. Kanaly, T. Hanaoka, H. Sugimura, H. Toda, S. Matsui

    The 34th Annual Meeting of the Japanese Environmental Mutagen Society (J-EMS)  2005.11 

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  • NAPL effects on benzo[a]pyrene biodegradation in soil by the indigenous microbiota and through bioaugmentation International conference

    Kanaly R. A, H.-G. Hur, K. Watanabe

    International Water Association (IWA) Conference on the Sustainable Development of Chemical Industries (Chemotox 2005)  2005.7 

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  • Absolute configuration-dependent epoxide formation from cis- and trans-isoflavan-4-ol steroisomers by biphenyl dioxygenase of Pseudomonas pseudoalcaligenes strain KF707 International conference

    Seo, J, S.-I. Kang, D. Won, M. Kim, J.-Y. Ryu, S.-W. Kang, B.-H. Um, C.-H. Pan, J.-H. Ahn, Y. Chong, R. A. Kanaly, J. Han, H.-G. Hur

    The Commemorative International Symposium for the 50th Anniversary of the Korean Society for Applied Biological Chemistry (KSABC)  2010.8 

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  • Biotransformation of the environmental pollutant, benzo[a]pyrene by a bacterium isolated from soil

    Izawa, M, R.A. Kanaly

    The Fifth Intercollegiate Conference on Science in Japan, Japan Ministry of Education, Culture, Sports, Science and Technology  2016.3 

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  • Investigation of benzo[a]pyrene biodegradation by a bacterium using gas chromatography and liquid chromatography electrospray ionization tandem mass spectrometry

    Izawa, M, R.A. Kanaly

    The Fourth Intercollegiate Conference on Science in Japan, Japan Ministry of Education, Culture, Sports, Science and Technology  2015.2 

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  • Investigation of intracellular RNA damage to improve chemical safety assessment methods

    Takeshita, T, R.A. Kanaly

    The Seventh Intercollegiate Conference on Science in Japan, Japan Ministry of Education, Culture, Sports, Science and Technology  2018.3 

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  • Detection of multiple DNA adducts in human lung and esophagus tissue by liquid chromatography electrospray ionization tandem mass spectrometry International conference

    Kanaly, R. A, S. Matsui, T. Hanaoka, T. Matsuda

    232nd American Chemical Society National Meeting  2006.9 

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  • Formation of sand star biofilm flocs by a member of the genus Sphingobium

    Tomiyama, Y, T. Takeshita, R.A. Kanaly

    The Seventh Intercollegiate Conference on Science in Japan, Japan Ministry of Education, Culture, Sports, Science and Technology  2018.3 

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Research Projects

  • 生体触媒による重質油改質に向けた異種細菌の協調的なアスファルテン生分解機構の理解

    Grant number:23K05013  2023.4 - 2026.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    Robert Kanaly, 守 次朗

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    Determined the biotransformation capabilities of Sphingobium bacterium when it was exposed to different polyalkyl chain-alkylated monoaromatic and polycyclic aromatic hydrocarbons (PAHs) and constructed biotransformation pathways for their biodegradation by comprehensive liquid chromatography electrospray ionization tandem mass spectrometry.
    <BR>
    Cooperative biodegradation of a model asphaltene long-chain PAH was investigated by a compartmentalized approach using bacteria belonging to the genera Pseudomonas and Sphingobium. Pseudomonas reduced the size (and molar mass) of 1-dodecylnaphthalene through subterminal alkyl chain cutting resulting in shorter alkyl chain polyaromatics that were further biotransformed by Sphingobium to ultimately produce aromatic ring-opened chain-shortened compounds.

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