Researcher Profile - Takehiko Ogawa

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写真a

Takehiko Ogawa

Organization

President,etc. Vice President

Profile

泌尿器科医・基礎医学研究者として横浜市立大学医学研究科において、精子形成の基礎および再生法の研究をおこなっている。培養下での精子形成法開発により、再生医療学会のJohnson & Johnson Innovation Award、読売テクノフォーラムのゴールドメダル賞の受賞歴がある。

External link

Degree

(BLANK) ( Yokohama City University )

Research Interests

アンドロロジー
再生医学
regenerative medicine
男性不妊症
spermatogenesis
Male infertility
andrology

Research Areas

Life Science / Urology
Life Science / Medical biochemistry / spermatogenesis

Education

Yokohama City University Graduate Graduate School of Medicine

1985 - 1989

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Yokohama City University Faculty of Medicine

- 1985

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Yokohama City University

1979 - 1985

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Country： Japan

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Committee Memberships

日本学術振興会学術システム研究センター研究員

2020.4 - 2024.3

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文部科学省科学技術・学術審議会専門委員

2019.3 - 2025.2

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Committee type：Academic society

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総合科学技術・イノベーション会議生命倫理専門調査会生命倫理専門調査会専門委員

2017.4

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Committee type：Government

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Japan Society for Reproductive Medicine Editorial Committee

2016.6

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Committee type：Academic society

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日本生殖内分泌学会理事

2015

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Committee type：Academic society

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日本受精着床学会評議員

2012

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Committee type：Academic society

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日本アンドロロジー学会評議員

2011

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Committee type：Academic society

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日本生殖発生医学会副理事

2008.3

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Committee type：Academic society

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Papers

Development of the membrane ceiling method for in vitro spermatogenesis Reviewed

Maki Kamoshita, Hiroki Shirai, Hiroko Nakamura, Tetsuya Kishimoto, Yuki Hatanaka, Daisuke Mashiko, Katsuhiro Esashika, Jingjing Yang, Satoshi Yamasaki, Takehiko Ogawa, Hiroshi Kimura, Masahito Ikawa

Scientific Reports 15 ( 1 ) 2025.1

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Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

Abstract

Spermatogenesis is one of the most complex processes of cell differentiation and its failure is a major cause of male infertility. Therefore, a proper model that recapitulates spermatogenesis in vitro has been long sought out for basic and clinical research. Testis organ culture using the gas-liquid interphase method has been shown to support spermatogenesis in mice and rats. However, the conventional method using agarose gel has limitations including medium replacement efficiency and live imaging because agarose absorbs medium and is not transparent. To overcome this issue, we developed a new device using microporous membranes and oxygen-permeable materials. Mouse testes sandwiched between a microporous polyethylene terephthalate (PET) membrane on top and an oxygen-permeable 4-polymethyl-1-pentene polymer (PMP) membrane base maintained spermatogenesis over months. The chamber volume was minimized to 0.1% of the culture medium. Weekly time-lapse live imaging enabled us to observe transgenically fluorescent acrosome and nuclear shape formation throughout spermatogenesis. Finally, we obtained healthy fertile offspring from spermatozoa generated in our system. The device could be used not only for basic research to understand spermatogenesis but also for applied research, such as diagnosing and treating male infertility.

DOI： 10.1038/s41598-024-84965-1

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Other Link： https://www.nature.com/articles/s41598-024-84965-1
A long-term mouse testis organ culture system to identify germ cell damage induced by chemotherapy. Reviewed International journal

Satoshi Yokota, Kiyoshi Hashimoto, Takuya Sato, Koichi Uemura, Kazuhide Makiyama, Takuya Nishimura, Satoshi Kitajima, Takehiko Ogawa

Current research in toxicology 8 100228 - 100228 2025

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Language：English Publishing type：Research paper (scientific journal)

We previously developed the acrosin-green fluorescent protein (GFP) transgenic neonatal mouse organ culture system for rapid and accurate assessment of testicular toxicity. This system effectively evaluates drug-induced toxicity in male germ cells before meiotic entry but cannot assess post-meiotic germ cell toxicity. For many chemicals, the specific stage of germ cell differentiation that is susceptible to toxicity remains unclear, highlighting the need for new methods. In this study, we incubated neonatal mouse testis organ cultures for 35 days to allow post-meiotic cells to develop. The tissue was then exposed to cisplatin to determine the cells that are targeted and to assess the reversibility of the toxicity. We monitored changes in tissue volume and GFP fluorescence, which tracks the progression of spermatogenesis, and confirmed findings by histological analysis. Cisplatin inhibited tissue growth and reduced GFP fluorescence in a concentration-dependent manner. Higher concentrations targeted not only spermatogonia, but also spermatocytes and spermatids. Recovery from toxicity was observed at clinically relevant doses. This study demonstrates that long-term mouse testis organ culture can be used to assess testicular toxicity, enabling the identification of specific germ cell stages targeted by chemicals such as cisplatin.

DOI： 10.1016/j.crtox.2025.100228

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Self-organization of spermatogenic wave coordinates sustained sperm production in the mouse testis

Toshiyuki Sato, Yuting I. Li, David J. Jörg, Mitsuru Komeya, Hiroyuki Yamanaka, Hiroko Nakamura, Kodai Hirano, Yohei Kondo, Kazuhiro Aoki, Masahide Takahashi, Hiroki Nakata, Hiroshi Kimura, Takehiko Ogawa, Benjamin D. Simons, Shosei Yoshida

2024.11

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Publisher：Cold Spring Harbor Laboratory

SUMMARY

Spermatogenesis takes place in the testis, relying on the ordered turnover of differentiating cells supplied from stem cells. Classic histological analyses have revealed that this process shows hierarchical spatiotemporal patterning known as the spermatogenic cycle, wave, and descent of segmental order, indicative of currently underexplored mechanisms of tissue- and organ-scale homeostasis. Here, using mice, we conducted high-resolution, wide-field, and ‘ultra’ long-term live imaging studiesin vivoandex vivo, combined with whole-organ mapping of differentiation stages. Such trans-scale measures demonstrate how stereotypic local cell turnover is coordinated into characteristic phase waves propagating along the seminiferous tubules, further organized into organ-scale patterning over the tubule loops. Minimal mathematical modeling shows that such higher-order dynamics can emerge from the local coupling of autonomous oscillators, which are rooted in delayed feedback interplay between stem and differentiating cells via retinoic acid signaling. These findings highlight a self-organization mechanism underpinning organ-scale homeostasis and constant sperm production.

DOI： 10.1101/2024.11.03.621757

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精巣器官培養の進化で広がる精子形成研究の未来

永田紫野, 小川毅彦, 佐藤卓也

臨床婦人科産科 78 ( 11 ) 1034 - 1039 2024.11

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Publishing type：Research paper (other academic)

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A novel alternative method for long‐term evaluation of male reproductive toxicity and its recovery using a pre‐pubertal mouse testis organ culture system

Kiyoshi Hashimoto, Hiroshi Arakawa, Rikako Imamura, Takuya Nishimura, Satoshi Kitajima, Takuya Sato, Kazuhide Makiyama, Takehiko Ogawa, Satoshi Yokota

Journal of Applied Toxicology 44 ( 5 ) 784 - 793 2024.1

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Publishing type：Research paper (scientific journal) Publisher：Wiley

Abstract

Successful treatment of pediatric cancers often results in long‐term health complications, including potential effects on fertility. Therefore, assessing the male reproductive toxicity of anti‐cancer drug treatments and the potential for recovery is of paramount importance. However, in vivo evaluations are time‐intensive and require large numbers of animals. To overcome these constraints, we utilized an innovative organ culture system that supports long‐term spermatogenesis by placing the testis tissue between a base agarose gel and a polydimethylsiloxane ceiling, effectively mirroring the in vivo testicular environment. The present study aimed to determine the efficacy of this organ culture system for accurately assessing testicular toxicity induced by cisplatin, using acrosin‐green fluorescent protein (GFP) transgenic neonatal mouse testes. The testis fragments were treated with different concentrations of cisplatin‐containing medium for 24 h and incubated in fresh medium for up to 70 days. The changes in tissue volume and GFP fluorescence over time were evaluated to monitor the progression of spermatogenesis, in addition to the corresponding histopathology. Cisplatin treatment caused tissue volume shrinkage and reduced GFP fluorescence in a concentration‐dependent manner. Recovery from testicular toxicity was also dependent on the concentration of cisplatin received. The results demonstrated that this novel in vitro system can be a faithful replacement for animal experiments to assess the testicular toxicity of anti‐cancer drugs and their reversibility, providing a useful method for drug development.

DOI： 10.1002/jat.4584

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Testis organ culture system capable of evaluating testicular toxicity

Kiyoshi Hashimoto, Hiroshi Arakawa, Rikako Imamura, Takuya Nishimura, Satoshi Kitajima, Takuya Sato, Kazuhide Makiyama, Takehiko Ogawa, Satoshi Yokota

2023.12

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Publisher：Cold Spring Harbor Laboratory

ABSTRACT

Successful treatment of pediatric cancers often results in long-term health complications, including potential effects on fertility. Therefore, assessing the male reproductive toxicity of anti-cancer drug treatments and the potential for recovery is of paramount importance. However,in vivoevaluations are time-intensive and require large numbers of animals. To overcome these constraints, we utilized an innovative organ culture system that supports long-term spermatogenesis by placing the testis tissue between a base agarose gel and a polydimethylsiloxane ceiling, effectively mirroring thein vivotesticular environment. The present study aimed to determine the efficacy of this organ culture system for accurately assessing testicular toxicity induced by cisplatin, using acrosin-green fluorescent protein (GFP) transgenic neonatal mouse testes. The testis fragments were treated with different concentrations of cisplatin-containing medium for 24 hours and incubated in fresh medium for up to 70 days. The changes in tissue volume and GFP fluorescence over time were evaluated to monitor the progression of spermatogenesis, in addition to the corresponding histopathology. Cisplatin treatment caused tissue volume shrinkage and reduced GFP fluorescence in a concentration-dependent manner. Recovery from testicular toxicity was also dependent on the concentration of cisplatin received. The results demonstrated that this novelin vitrosystem can be a faithful replacement for animal experiments to assess the testicular toxicity of anti-cancer drugs and their reversibility, providing a useful method for drug development.

Short Abstract

Assessing the male reproductive toxicity of anti-cancer drugs and the potential for recovery is of paramount importance, however,in vivoevaluations are time-intensive and require large numbers of animals. We utilized an innovative organ culture system that mirrors thein vivotesticular environment to determine its efficacy in accurately assessing testicular toxicity induced by cisplatin. The results demonstrate that this system can be a faithful replacement for animal experiments to assess the testicular toxicity of anti-cancer drugs and their reversibility.

DOI： 10.1101/2023.12.01.569566

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Culture-space control is effective in promoting haploid cell formation and spermiogenesis in vitro in neonatal mice

Kiyoshi Hashimoto, Hisakazu Odaka, Yu Ishikawa-Yamauchi, Shino Nagata, Hiroko Nakamura, Hiroshi Kimura, Takuya Sato, Kazuhide Makiyama, Takehiko Ogawa

Scientific Reports 13 ( 1 ) 2023.12

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Publishing type：Research paper (scientific journal)

DOI： 10.1038/s41598-023-39323-y

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Generation of rat offspring using spermatids produced through in vitro spermatogenesis

Takafumi Matsumura, Kumiko Katagiri, Tatsuma Yao, Yu Ishikawa-Yamauchi, Shino Nagata, Kiyoshi Hashimoto, Takuya Sato, Hiroshi Kimura, Takashi Shinohara, Makoto Sanbo, Masumi Hirabayashi, Takehiko Ogawa

SCIENTIFIC REPORTS 13 ( 1 ) 2023.7

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1038/s41598-023-39304-1

Web of Science

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In vitro spermatogenesis in isolated seminiferous tubules of immature mice

Xuemin Feng, Takafumi Matsumura, Yuki Yamashita, Takuya Sato, Kiyoshi Hashimoto, Hisakazu Odaka, Yoshinori Makino, Yuki Okada, Hiroko Nakamura, Hiroshi Kimura, Teruo Fujii, Takehiko Ogawa

PLOS ONE 18 ( 4 ) e0283773 - e0283773 2023.4

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Publishing type：Research paper (scientific journal) Publisher：Public Library of Science (PLoS)

Mouse spermatogenesis, from spermatogonial stem cell proliferation to sperm formation, can be reproduced in vitro by culturing testis tissue masses of neonatal mice. However, it remains to be determined whether this method is also applicable when testis tissues are further divided into tiny fragments, such as segments of the seminiferous tubule (ST), a minimal anatomical unit for spermatogenesis. In this study, we investigated this issue using the testis of an Acrosin-GFP/Histone H3.3-mCherry (Acr/H3) double-transgenic mouse and monitored the expression of GFP and mCherry as indicators of spermatogenic progression. Initially, we noticed that the cut and isolated stretches of ST shrunk rapidly and conglomerated. We therefore maintained the isolation of STs in two ways: segmental isolation without truncation or embedding in soft agarose. In both cases, GFP expression was observed by fluorescence microscopy. By whole-mount immunochemical staining, meiotic spermatocytes and round and elongating spermatids were identified as Sycp3-, crescent-form GFP-, and mCherry-positive cells, respectively. Although the efficiency was significantly lower than that with tissue mass culture, we clearly showed that spermatogenesis can be induced up to the elongating spermatid stage even when the STs were cut into short segments and cultured in isolation. In addition, we demonstrated that lowered oxygen tension was favorable for spermatogenesis both for meiotic progression and for producing elongating spermatids in isolated STs. Culturing isolated STs rather than tissue masses is advantageous for explicitly assessing the various environmental parameters that influence the progression of spermatogenesis.

DOI： 10.1371/journal.pone.0283773

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【不妊症・不育症治療の現在と未来】男性不妊症治療の未来

小川毅彦, 古目谷暢, 佐藤卓也

Medical Science Digest 49 ( 2 ) 70 - 73 2023.2

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Language：Japanese Publisher：(株)ニュー・サイエンス社

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Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control

Takehiko OGAWA, Takafumi MATSUMURA, Tatsuma YAO, Hiroshi KIMURA, Kiyoshi HASHIMOTO, Yu ISHIKAWA-YAMAUCHI, Takuya SATO

Journal of Reproduction and Development 2023

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Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Animal Reproduction

DOI： 10.1262/jrd.2023-093

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シリコーン・チップを用いた培養法によるマウス精巣組織の成長

橋本雪司, 中村寛子, 木村啓志, 小高久和, 古目谷暢, 松村貴史, 佐藤卓也, 小川毅彦

泌尿器外科 35 ( 臨増 ) 817 - 817 2022.7

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Language：Japanese Publisher：医学図書出版(株)

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Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis

Kodai Hirano, Yuta Nonami, Yoshiaki Nakamura, Toshiyuki Sato, Takuya Sato, Kei-ichiro Ishiguro, Takehiko Ogawa, Shosei Yoshida

Communications Biology 5 ( 1 ) 2022.5

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Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

Abstract

Mammalian spermatogenesis is a heat-vulnerable process that occurs at low temperatures, and elevated testicular temperatures cause male infertility. However, the current reliance on in vivo assays limits their potential to detail temperature dependence and destructive processes. Using ex vivo cultures of mouse testis explants at different controlled temperatures, we found that spermatogenesis failed at multiple steps, showing sharp temperature dependencies. At 38 °C (body core temperature), meiotic prophase I is damaged, showing increased DNA double-strand breaks (DSBs) and compromised DSB repair. Such damaged spermatocytes cause asynapsis between homologous chromosomes and are eliminated by apoptosis at the meiotic checkpoint. At 37 °C, some spermatocytes survive to the late pachytene stage, retaining high levels of unrepaired DSBs but do not complete meiosis with compromised crossover formation. These findings provide insight into the mechanisms and significance of heat vulnerability in mammalian spermatogenesis.

DOI： 10.1038/s42003-022-03449-y

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Other Link： https://www.nature.com/articles/s42003-022-03449-y
In vitro reconstitution of the whole male germ-cell development from mouse pluripotent stem cells International journal

Yukiko Ishikura, Hiroshi Ohta, Takuya Sato, Yusuke Murase, Yukihiro Yabuta, Yoji Kojima, Chika Yamashiro, Tomonori Nakamura, Takuya Yamamoto, Takehiko Ogawa, Mitinori Saitou

Cell Stem Cell 28 ( 12 ) 2167 - 2179.e9 2021.12

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Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

Mammalian male germ-cell development consists of three distinct phases: primordial germ cell (PGC) development, male germ-cell specification for spermatogonium development, and ensuing spermatogenesis. Here, we show an in vitro reconstitution of whole male germ-cell development by pluripotent stem cells (PSCs). Mouse embryonic stem cells (mESCs) are induced into PGC-like cells (mPGCLCs), which are expanded for epigenetic reprogramming. In reconstituted testes under an optimized condition, such mPGCLCs differentiate into spermatogonium-like cells with proper developmental transitions, gene expression, and cell-cycle dynamics and are expanded robustly as germline stem cell-like cells (GSCLCs) with an appropriate androgenetic epigenome. Importantly, GSCLCs show vigorous spermatogenesis, not only upon transplantation into testes in vivo but also under an in vitro culture of testis transplants, and the resultant spermatids contribute to fertile offspring. By uniting faithful recapitulations of the three phases of male germ-cell development, our study creates a paradigm for the in vitro male gametogenesis by PSCs.

DOI： 10.1016/j.stem.2021.08.005

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シリコーン・チップを用いた培養法によるマウス精巣組織の成長と体外精子形成

橋本雪司, 中村寛子, 木村啓志, 小高久和, 古目谷暢, 松村貴史, 佐藤卓也, 小川毅彦

日本泌尿器科学会総会 109回 PP66 - 03 2021.12

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Language：Japanese Publisher：(一社)日本泌尿器科学会総会事務局

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Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control Reviewed

Takafumi Matsumura, Takuya Sato, Takeru Abe, Hiroyuki Sanjo, Kumiko Katagiri, Hiroshi Kimura, Teruo Fujii, Hiromitsu Tanaka, Masumi Hirabayashi, Takehiko Ogawa

Scientific Reports 11 ( 1 ) 2021.12

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Authorship：Corresponding author Publishing type：Research paper (scientific journal)

DOI： 10.1038/s41598-021-82792-2

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Sertoli cell replacement in explanted mouse testis tissue supporting host spermatogenesis† Reviewed International journal

Kazusa Higuchi, Takafumi Matsumura, Haruhiko Akiyama, Yoshiakira Kanai, Takehiko Ogawa, Takuya Sato

Biology of Reproduction 105 ( 4 ) 934 - 943 2021.10

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Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

<title>Abstract</title>
Spermatogenesis takes place in the seminiferous tubules, starting from the spermatogonial stem cell and maturing into sperm through multiple stages of cell differentiation. Sertoli cells, the main somatic cell constituting the seminiferous tubule, are in close contact with every germ cell and play pivotal roles in the progression of spermatogenesis. In this study, we developed an in vitro Sertoli cell replacement method by combining an organ culture technique and a toxin receptor-mediated cell knockout system. We used Amh-diphtheria toxin receptor transgenic mice, whose Sertoli cells specifically express human diphtheria toxin receptor, which renders them sensitive to diphtheria toxin. An immature Amh-diphtheria toxin receptor testis was transplanted with the donor testis cells followed by culturing in a medium containing diphtheria toxin. This procedure successfully replaced the original Sertoli cells with the transplanted Sertoli cells, and spermatogenesis originating from resident germ cells was confirmed. In addition, Sertoli cells in the mouse testis tissues were replaced by transplanted rat Sertoli cells within culture conditions without requiring immunosuppressive treatments. This method works as a functional assay system, making it possible to evaluate any cells that might function as Sertoli cells. It would also be possible to investigate interactions between Sertoli and germ cells more closely, providing a new platform for the study of spermatogenesis and its impairments.

DOI： 10.1093/biolre/ioab104

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Other Link： http://academic.oup.com/biolreprod/article-pdf/105/4/934/40545414/ioab104.pdf
Gap between UAS and ureteroscope predicts renal stone-free rate after flexible ureteroscopy with the fragmentation technique. International journal

Mitsuru Komeya, Hisakazu Odaka, Takahiko Watanabe, Hirokazu Kiuchi, Takehiko Ogawa, Masahiro Yao, Junichi Matsuzaki

World journal of urology 39 ( 7 ) 2733 - 2739 2021.7

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Language：English Publishing type：Research paper (scientific journal)

PURPOSE: To assess the effect of our new classification on surgical outcomes after flexible ureteroscopy (fURS) for kidney stones. METHODS: We retrospectively examined 128 patients after single renal fURS procedures performed using ureteral access sheaths (UASs) with the fragmentation technique. Based on the gap (calculated by subtracting the ureteroscope diameter from the UAS diameter), enrolled patients were divided into three groups: small (< 0.6 mm), medium (0.6 to < 1.2 mm), and large space groups (≥ 1.2 mm). Stone-free (SF) status was defined as either complete absence of stones (SF) or the presence of stones < 4 mm in diameter on non-contrast computed tomography (NCCT). RESULTS: The SF rate was significantly lower in the small space group (50% in small, 97.9% in medium, 89.2% in large; p = 0.001). Perioperative complications over Clavien-Dindo Grade I were observed in 16.7%, 4.2%, and 8.1% of patients, respectively (p = 0.452). The ratio of stone volume and operative time (efficiency of stone removal) was significantly higher in the large space group compared to the small and medium space groups (0.009 ± 0.003 ml/min, 0.013 ± 0.005 ml/min, 0.027 ± 0.012 ml/min, respectively; p < 0.001). CONCLUSION: Our findings that gaps > 0.6 mm (1.8 Fr), including the combination of a 9.5-Fr UAS and a small caliber ureteroscope, improve SF rates, and larger gaps facilitate stone removal efficiency providing the basis for future development of clinical protocols aimed at improving outcomes.

DOI： 10.1007/s00345-020-03459-7

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Time-course microarray transcriptome data of in vitro cultured testes and age-matched in vivo testes Reviewed International journal

Takeru Abe, Hajime Nishimura, Takuya Sato, Harukazu Suzuki, Takehiko Ogawa, Takahiro Suzuki

Data in Brief 33 106482 - 106482 2020.12

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Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

In vitro spermatogenesis, which produces fertile spermatozoa, has been successfully performed using an organ culture method from murine tissue. Here, we provide a dataset of time-course microarray transcriptome data of in vitro cultured neonate murine testes and age-matched in vivo-derived testes. The dataset presented here is related to the article titled "Transcriptome analysis reveals inadequate spermatogenesis and immediate radical immune reactions during organ culture in vitro spermatogenesis" published in Biochemical and Biophysical Research Communications in 2020 [1]. The raw data and pre-processed data are publicly available on the GEO repository (accession number GSE147982). Furthermore, the dataset provided here includes additional metadata, detailed explanations of the experiment, results of pre-processing, analysis scripts, and lists of differentially expressed genes from in vitro culture testes and in vivo testes at each time point.

DOI： 10.1016/j.dib.2020.106482

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In vitro精子形成の研究最前線

橋本雪司, 松村貴史, 古目谷暢, 佐藤卓也, 小川毅彦

泌尿器科 12 ( 5 ) 576 - 584 2020.11

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Language：Japanese Publisher：(有)科学評論社

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Transcriptome analysis reveals inadequate spermatogenesis and immediate radical immune reactions during organ culture in vitro spermatogenesis Reviewed International journal

Takeru Abe, Hajime Nishimura, Takuya Sato, Harukazu Suzuki, Takehiko Ogawa, Takahiro Suzuki

Biochemical and Biophysical Research Communications 530 ( 4 ) 732 - 738 2020.10

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Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

Cultivation of neonatal mouse testis tissue can induce spermatogenesis and produce fertile sperms. However, in vitro spermatogenesis mediated by the current organ culture method comes short in fully mimicking the in vivo counterpart, partly due to a lack of knowledge underlying molecular phenotypes of in vitro spermatogenesis. In this study, we investigated transcriptome of cultured testis tissues using microarray method. Principle component analysis of the transcriptome data revealed delay and/or arrest of spermatogenesis and immediate radical immune reactions in the cultured testis tissues. The delay/arrest of spermatogenesis occurred before and during early meiotic phase, resulting in inefficient progression of meiosis. The immune reaction, on the other hand, was drastic and overwhelming, in which TLR4-NF-kB signaling was speculated to be involved. Notably, treatment with TAK242, an inhibitor of TLR4-NF-kB signaling pathway, ameliorated the macrophage activation which otherwise would exacerbate the inflammation. Thus, the present study revealed for the first time at molecular level that the deficiency of germ cell differentiation and the immense immune reaction are major abnormalities in the cultured testis tissues.

DOI： 10.1016/j.bbrc.2020.06.161

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Development and internal validation of a nomogram to predict perioperative complications after flexible ureteroscopy for renal stones in overnight ureteral catheterization cases. International journal

Mitsuru Komeya, Hisakazu Odaka, Jun Asano, Takuo Asai, Yusuke Saigusa, Takehiko Ogawa, Masahiro Yao, Junichi Matsuzaki

World journal of urology 38 ( 9 ) 2307 - 2312 2020.9

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Language：English Publishing type：Research paper (scientific journal)

PURPOSE: To identify risk factors by developing and internally validating a nomogram for preventing perioperative complications in overnight ureteral catheterization cases after fURS for kidney stones. METHODS: We retrospectively examined 309 patients with overnight ureteral catheterization after single fURS procedures for renal stones. fURS procedures were performed based on the fragmentation technique. The ureteral catheter was removed on postoperative day 1. Within this group, patients who experienced perioperative complications (complication group) were compared with those who did not experience complications (non-complication group). The complication group included 77 patients whose Clavien-Dindo classification score was I, II, III, or IV and/or those whose body temperature during hospitalization was over 37.5 °C. RESULTS: The overall stone volume, stone-free rate, incidence of perioperative complications, and procedure duration were 1.39 mL, 94.8%, 24.9%, and 62 min, respectively. Severe complications of a Clavien-Dindo level III or IV were observed in only four cases (1.3%). Multivariate assessment revealed five independent predictors of perioperative complications after fURS with overnight catheterization: age (p = 0.11), sex (p = 0.067), stone volume (p = 0.33), Hounsfield units (p = 0.16), and narrow ureter (p = 0.018). We developed a nomogram to predict perioperative complications after fURS using these parameters. CONCLUSIONS: We developed a predictive model for perioperative complications of patients with overnight catheterization after fURS for renal stones. This model could select patients who were at a low risk of complications.

DOI： 10.1007/s00345-019-03023-y

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Spatially Fractionated Microbeam Analysis of Tissue-sparing Effect for Spermatogenesis

Hisanori Fukunaga, Kiichi Kaminaga, Takuya Sato, Karl T. Butterworth, Ritsuko Watanabe, Noriko Usami, Takehiko Ogawa, Akinari Yokoya, Kevin M. Prise

Radiation Research 194 ( 6 ) 2020.8

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Publishing type：Research paper (scientific journal) Publisher：Radiation Research Society

DOI： 10.1667/rade-19-00018.1

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Antioxidant vitamins and lysophospholipids are critical for inducing mouse spermatogenesis under organ culture conditions Reviewed

Hiroyuki Sanjo, Tatsuma Yao, Kumiko Katagiri, Takuya Sato, Takafumi Matsumura, Mitsuru Komeya, Hiroyuki Yamanaka, Masahiro Yao, Akio Matsuhisa, Yuta Asayama, Kazutaka Ikeda, Kuniyuki Kano, Junken Aoki, Makoto Arita, Takehiko Ogawa

FASEB Journal 34 ( 7 ) 9480 - 9497 2020.7

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Publishing type：Research paper (scientific journal)

DOI： 10.1096/fj.202000245R

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Minimally invasive versus standard endoscopic combined intrarenal surgery for renal stones: a retrospective pilot study analysis. International journal

Kimitsugu Usui, Mitsuru Komeya, Masataka Taguri, Koshi Kataoka, Takuo Asai, Takehiko Ogawa, Masahiro Yao, Junichi Matsuzaki

International urology and nephrology 52 ( 7 ) 1219 - 1225 2020.7

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PURPOSE: The effect of combining miniaturization with endoscopic combined intrarenal surgery (ECIRS) is unclear. Thus, we compared the treatment outcomes between minimally invasive ECIRS (mini-ECIRS) using 16.5 Fr percutaneous access sheath and standard ECIRS using 24 Fr access sheath for renal stones MATERIALS AND METHODS: We retrospectively analyzed consecutive patients who underwent single session mini or standard-ECIRS in the modified Valdivia position for renal stones between April 2009 and May 2016. To adjust for patient characteristics, 77 pairs were matched using preoperative parameters including age, sex, history of febrile urinary tract infection (UTI), stone surface area, number of involved calyces, and staghorn calculi. RESULTS: The stone free rate (SFR) was similar between mini and standard ECIRS according to non-contrast computed tomography (61.1% vs. 52.0%, p = 0.388). The rate of perioperative complications exceeding grade 2 based on the Clavien-Dindo classification was similar in both groups (19.5% vs. 26.0%, p = 0.442). Severe complications exceeding grade 3 were also similar in both groups (2.6% vs. 3.9%, p > 0.99). Two cases of septic shock were noted in each group. Although there was no difference regarding bleeding-related complications (2.6% vs. 6.5%, p = 0.442), pseudoaneurysm or blood transfusion was not observed in the mini-ECIRS group. Pain visual analog scale values in the perioperative period were lower in the mini-ECIRS group (1.34 ± 1.08 vs. 1.69 ± 1.23, p = 0.062). CONCLUSIONS: This study demonstrated that, compared to standard ECIRS, mini-ECIRS maintained SFR without increasing perioperative complications, tended to reduce postoperative pain and had a potential to reduce bleeding-related complications. This report suggests the advantages of ECIRS miniaturization for renal stones.

DOI： 10.1007/s11255-020-02433-x

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The Tissue-Sparing Effect of Spatially Fractionated X-rays for Maintaining Spermatogenesis: A Radiobiological Approach for the Preservation of Male Fertility after Radiotherapy. Reviewed International journal

Hisanori Fukunaga, Kiichi Kaminaga, Takuya Sato, Ritsuko Watanabe, Takehiko Ogawa, Akinari Yokoya, Kevin M Prise

Journal of clinical medicine 9 ( 4 ) 2020.4

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Radiotherapy can result in temporary or permanent gonadal toxicity in male cancer patients despite the high precision and accuracy of modern radiation treatment techniques. Previous radiobiological studies have shown an effective tissue-sparing response in various tissue types and species following exposure to spatially fractionated radiation. In the present study, we used an ex vivo mouse testicular tissue culture model and a conventional X-ray irradiation device to evaluate the tissue-sparing effect (TSE) of spatially fractionated X-rays for the protection of male fertility from radiotherapy-related adverse effects. We revealed a significant TSE for maintaining spermatogenesis in the ex vivo testes model following spatially fractionated X-ray irradiation. Moreover, we experimentally propose a possible mechanism by which the migration of spermatogonial cells, from the non-irradiated areas to the irradiated ones, in irradiated testicular tissue, is essential for the TSE and maintaining spermatogenesis. Therefore, our findings demonstrate that the control of TSE following spatially fractionated X-rays in the testes has a considerable potential for clinical application. Interdisciplinary research will be essential for further expanding the applicability of this method as an approach for the preservation of male fertility during or after radiotherapy.

DOI： 10.3390/jcm9041089

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精巣組織培養法を用いたin vitroラット精子形成系の開発

松村貴史, 片桐久美子, 三條博之, 安部丈, 古目谷暢, 佐藤卓也, 田中宏光, 平林真澄, 小川毅彦

日本内分泌学会雑誌 95 ( 4 ) 1554 - 1554 2020.2

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単離精細管での培養下マウス精子形成

フン・シェミン, 松村貴史, 片桐久美子, 中村寛子, 木村啓志, 古目谷暢, 佐藤卓也, 小川毅彦

日本内分泌学会雑誌 95 ( 4 ) 1558 - 1558 2020.2

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High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation. Reviewed

Fukunaga H, Kaminaga K, Sato T, Butterworth KT, Watanabe R, Usami N, Ogawa T, Yokoya A, Prise KM

Sci Rep 9 ( 1 ) 12618 2019.10

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Live Offspring after Testis Tissue Transplantation. Invited

OGAWA Takehiko

N Engl J Med 381 ( 15 ) 1477 - 1479 2019.10

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静水圧を利用した簡易なマイクロ流体デバイスによる精巣組織培養について

山中弘行, 古目谷暢, 小川毅彦

日本生殖医学会雑誌 64 ( 4 ) 408 - 408 2019.10

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In vitro spermatogenesis in two-dimensionally-spread mouse testis tissues. Reviewed

Komeya M, Yamanaka H, Sanjo H, Yao M, Nakamura H, Kimura H, Fujii T, Sato T, Ogawa T

Reprod Med Biol. 18 ( 4 ) 362 - 369 2019.10

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泌尿器科領域における基礎研究の成果 In vitro精子形成システムの開発

古目谷暢, 小川毅彦, 矢尾正祐

泌尿器外科 32 ( 臨増 ) 592 - 594 2019.6

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静水圧を利用した簡易なマイクロ流体デバイスによる精巣組織培養について

山中弘行, 古目谷暢, 中村寛子, 木村啓志, 小川毅彦, 矢尾正祐

日本泌尿器科学会総会 107回 PP1 - 305 2019.4

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マイクロ流体器官培養法を用いた長期精巣ライブイメージング系の開発

佐藤俊之, 吉田松生, 古目谷暢, 山中弘行, 小川毅彦, 中村寛子, 木村啓志, 高橋雅英

日本病理学会会誌 108 ( 1 ) 497 - 497 2019.4

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Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method. Reviewed

OGAWA Takehiko

Sci Rep. 1874 87 - 98 2019

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Neonatal testis growth recreated in vitro by two-dimensional organ spreading Reviewed

Kazuaki Kojima, Hiroko Nakamura, Mitsuru Komeya, Hiroyuki Yamanaka, Yoshinori Makino, Yuki Okada, Haruhiko Akiyama, Nobuhito Torikai, Takuya Sato, Teruo Fujii, Hiroshi Kimura, Takehiko Ogawa

Biotechnology and Bioengineering 115 ( 12 ) 3030 - 3041 2018.12

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DOI： 10.1002/bit.26822

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器官培養法によるマウス精子完成過程の検討

鳥飼啓人, 三條博之, 山中弘行, 古目谷暢, 佐藤卓也, 小川毅彦

日本内分泌学会雑誌 94 ( 4 ) 1444 - 1444 2018.12

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器官培養法によるマウス精子完成過程の再現

鳥飼啓人, 三條博之, 山中弘行, 古目谷暢, 小川毅彦

日本生殖医学会雑誌 63 ( 3 ) 313 - 313 2018.8

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In vitro spermatogenesis: A century-long research journey, still half way around. Reviewed

Komeya M, Sato T, Ogawa T

Reprod Med Biol. 17 ( 4 ) 407 - 420 2018.8

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Effect of fluid shear stress on in vitro cultured ureteric bud cells. Reviewed

Kimura H, Nishikawa M, Yanagawa N, Nakamura H, Miyamoto S, Hamon M, Hauser P, Zhao L, Jo OD, Komeya M, Ogawa T, Yanagawa N

Biomicrofluidics 12 ( 4 ) 044107 2018.7

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A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility Reviewed

Hiroyuki Yamanaka, Mitsuru Komeya, Hiroko Nakamura, Hiroyuki Sanjo, Takuya Sato, Masahiro Yao, Hiroshi Kimura, Teruo Fujii, Takehiko Ogawa

Biochemical and Biophysical Research Communications 500 ( 4 ) 885 - 891 2018.6

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DOI： 10.1016/j.bbrc.2018.04.180

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Application of an Ex Vivo Tissue Model to Investigate Radiobiological Effects on Spermatogenesis. Reviewed

Fukunaga H, Kaminaga K, Sato T, Usami N, Watanabe R, Butterworth KT, Ogawa T, Yokoya A, Prise KM

Radiation research 189 ( 6 ) 661 - 667 2018.6

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Outcome of flexible ureteroscopy for renal stone with overnight ureteral catheterization: a propensity score-matching analysis. Reviewed

Komeya M, Usui K, Asai T, Ogawa T, Taguri M, Kataoka K, Yao M, Matsuzaki J

World journal of urology 2018.5

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マイクロ流体デバイスを用いたIn Vitro精巣組織培養法の改良

山中弘行, 古目谷暢, 中村寛子, 木村啓志, 小川毅彦, 矢尾正祐

日本泌尿器科学会総会 106回 PP3 - 228 2018.4

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シリコン樹脂カバーを併用した精巣組織培養法による精子形成効率の向上

古目谷暢, 山中弘行, 三條博之, 佐藤卓也, 片桐久美子, 木村啓志, 藤井輝夫, 矢尾正祐, 小川毅彦

日本泌尿器科学会総会 106回 OP - 264 2018.4

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In vitro mouse spermatogenesis with an organ culture method in chemically defined medium Reviewed

Hiroyuki Sanjo, Mitsuru Komeya, Takuya Sato, Takeru Abe, Kumiko Katagiri, Hiroyuki Yamanaka, Yoko Ino, Noriaki Arakawa, Hisashi Hirano, Tatsuma Yao, Yuta Asayama, Akio Matsuhisa, Masahiro Yao, Takehiko Ogawa

PLoS ONE 13 ( 2 ) e0192884 2018.2

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DOI： 10.1371/journal.pone.0192884

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Low-dose radiation-induced risk in spermatogenesis. Reviewed International coauthorship International journal

Fukunaga H, Butterworth KT, Yokoya A, Ogawa T, Prise KM

International Journal of Radiation Biology 93 ( 12 ) 1291 - 1298 2017.12

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DOI： 10.1080/09553002.2017.1355579

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Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro Reviewed

Mitsuru Komeya, Kazuaki Hayashi, Hiroko Nakamura, Hiroyuki Yamanaka, Hiroyuki Sanjo, Kazuaki Kojima, Takuya Sato, Masahiro Yao, Hiroshi Kimura, Teruo Fujii, Takehiko Ogawa

SCIENTIFIC REPORTS 7 ( 1 ) 15459 2017.11

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DOI： 10.1038/s41598-017-15799-3

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シリコン樹脂カバーを用いた精巣組織培養法による精子形成効率の改善に向けた検討

古目谷暢, 山中弘行, 三條博之, 小川毅彦

日本生殖医学会雑誌 62 ( 4 ) 458 - 458 2017.10

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化学組成の明らかな培養液を用いた体外精巣器官培養法

三條博之, 山中弘行, 古目谷暢, 小川毅彦

日本生殖医学会雑誌 62 ( 4 ) 428 - 428 2017.10

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マイクロ流体デバイスを用いたIn Vitro精巣組織培養法の改良

山中弘行, 古目谷暢, 加藤佐樹子, 林功晃, 中村寛子, 木村啓志, 小川毅彦, 矢尾正祐

日本生殖医学会雑誌 62 ( 4 ) 457 - 457 2017.10

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A noncoding RNA containing a SINE-B1 motif associates with meiotic metaphase chromatin and has an indispensable function during spermatogenesis Reviewed

Ryusuke Nakajima, Takuya Sato, Takehiko Ogawa, Hideyuki Okano, Toshiaki Noce

PLOS ONE 12 ( 6 ) e0179585 2017.6

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DOI： 10.1371/journal.pone.0179585

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マイクロ流体デバイスを用いたIn Vitro精子形成について

山中弘行, 古目谷暢, 加藤佐樹子, 林功晃, 中村寛子, 木村啓志, 小川毅彦, 矢尾正祐

日本泌尿器科学会総会 105回 PP56 - 03 2017.4

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Evaluation of Culture Time and Media in an In Vitro Testis Organ Culture System Reviewed

Noriko Nakamura, Gwenn E. Merry, Amy L. Inselman, Daniel T. Sloper, Pedro L. Del Valle, Takuya Sato, Takehiko Ogawa, Deborah K. Hansen

BIRTH DEFECTS RESEARCH 109 ( 7 ) 465 - 474 2017.4

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DOI： 10.1002/bdr2.1002

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SHISA6 Confers Resistance to Differentiation-Promoting Wnt/beta-Catenin Signaling in Mouse Spermatogenic Stem Cells Reviewed

Moe Tokue, Kanako Ikami, Seiya Mizuno, Chiyo Takagi, Asuka Miyagi, Ritsuko Takada, Chiyo Noda, Yu Kitadate, Kenshiro Hara, Hiroko Mizuguchi, Takuya Sato, Makoto Mark Taketo, Fumihiro Sugiyama, Takehiko Ogawa, Satoru Kobayashi, Naoto Ueno, Satoru Takahashi, Shinji Takada, Shosei Yoshida

STEM CELL REPORTS 8 ( 3 ) 561 - 575 2017.3

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DOI： 10.1016/j.stemcr.2017.01.006

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マイクロ流体システムを用いた次世代型in vitro精子形成システムの開発とその応用

山中弘行, 三條博之, 古目谷暢, 小川毅彦

日本生殖医学会雑誌 61 ( 4 ) 448 - 448 2016.10

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マイクロ流体システムを用いて生体内での精子形成を再現した長期培養システムの開発と実用化促進に向けた検討

古目谷暢, 山中弘行, 三條博之, 小川毅彦

日本生殖医学会雑誌 61 ( 4 ) 349 - 349 2016.10

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Spermatogenesis in Explanted Fetal Mouse Testis Tissues Reviewed

Kazuaki Kojima, Takuya Sato, Yuta Naruse, Takehiko Ogawa

BIOLOGY OF REPRODUCTION 95 ( 3 ) 2016.9

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DOI： 10.1095/biolreprod.116.140277

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精子形成を長期間維持できるin vitro精巣組織培養システムの開発

古目谷暢, 林功晃, 山中弘行, 三條博之, 小島一晃, 片桐久美子, 佐藤卓也, 矢尾正祐, 木村啓志, 藤井輝夫, 小川毅彦, 小川毅彦

日本生殖内分泌学会雑誌 21 27‐31 - 31 2016.8

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透析機能を内蔵した精巣組織培養装置による新規精子形成メカニズムの解明

小笠原恵理, 古目谷暢, 古目谷暢, 林功晃, 山中弘行, 佐藤卓也, 三條博之, 矢尾正祐, 木村啓志, 藤井輝夫, 小川毅彦, 小川毅彦

日本アンドロロジー学会総会記事 35th 165‐166 2016.6

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腎泌尿器疾患と酸化ストレス酸化ストレスと精巣

古目谷暢, 小川毅彦

日本抗加齢医学会総会プログラム・抄録集 16回 86 - 86 2016.6

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精子無力症患者における精子プロテオーム解析

三條博之, 荒川憲昭, 湯村寧, 矢尾正祐, 小川毅彦

日本泌尿器科学会総会 104回 PP2 - 126 2016.4

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Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device Reviewed

Mitsuru Komeya, Hiroshi Kimura, Hiroko Nakamura, Tetsuhiro Yokonishi, Takuya Sato, Kazuaki Kojima, Kazuaki Hayashi, Kumiko Katagiri, Hiroyuki Yamanaka, Hiroyuki Sanjo, Masahiro Yao, Satoshi Kamimura, Kimiko Inoue, Narumi Ogonuki, Atsuo Ogura, Teruo Fujii, Takehiko Ogawa

SCIENTIFIC REPORTS 6 21472 2016.2

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DOI： 10.1038/srep21472

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Cryopreservation of testis tissues and in vitro spermatogenesis Reviewed

Tetsuhiro Yokonishi, Takehiko Ogawa

Reproductive Medicine and Biology 15 ( 1 ) 21 - 28 2016.1

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DOI： 10.1007/s12522-015-0218-4

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Spermatogonial stem cells: progress and prospects Reviewed

Mitsuru Komeya, Takehiko Ogawa

ASIAN JOURNAL OF ANDROLOGY 17 ( 5 ) 771 - 775 2015.9

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DOI： 10.4103/1008-682X.154995

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精子形成を長期間維持できるin vitro精巣組織培養システムの開発

古目谷暢, 林功晃, 山中弘行, 三條博之, 小島一晃, 片桐久美子, 佐藤卓也, 矢尾正祐, 木村啓志, 藤井輝夫, 小川毅彦

日本内分泌学会雑誌 91 ( 2 ) 602 - 602 2015.9

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Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9 Reviewed

Takuya Sato, Tetsushi Sakuma, Tetsuhiro Yokonishi, Kumiko Katagiri, Satoshi Kamimura, Narumi Ogonuki, Atsuo Ogura, Takashi Yamamoto, Takehiko Ogawa

STEM CELL REPORTS 5 ( 1 ) 75 - 82 2015.7

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DOI： 10.1016/j.stemcr.2015.05.011

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【腎泌尿器の抗酸化とアンチエイジング】腎泌尿器疾患と酸化ストレス酸化ストレスと精巣

古目谷暢, 小川毅彦

腎臓内科・泌尿器科 2 ( 1 ) 27 - 33 2015.7

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In Vitro Spermatogenesis in Explanted Adult Mouse Testis Tissues Reviewed

Takuya Sato, Kumiko Katagiri, Kazuaki Kojima, Mitsuru Komeya, Masahiro Yao, Takehiko Ogawa

PLOS ONE 10 ( 6 ) e0130171 2015.6

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DOI： 10.1371/journal.pone.0130171

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Andrology研究の最前線精巣組織培養法

古目谷暢, 横西哲広, 佐藤卓也, 片桐久美子, 木村啓志, 藤井輝夫, 小川毅彦, 矢尾正祐

日本泌尿器科学会総会 103回 383 - 383 2015.4

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生体内環境を疑似的に再現した次世代型in vitro精子形成システムの開発

古目谷暢, 横西哲広, 佐藤卓也, 片桐久美子, 木村啓志, 藤井輝夫, 小川毅彦, 矢尾正祐

日本泌尿器科学会総会 103回 481 - 481 2015.4

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前立腺肥大症(BPH)に伴う下部尿路症状(LUTS)に対するナフトピジル75mg/日の早期効果・安全性に関する検討

小泉充之, 野口和美, 花井孝宏, 石田寛明, 服部裕介, 寺西淳一, 湯村寧, 三好康秀, 近藤慶一, 佐野太, 林成彦, 中井川昇, 小川毅彦

泌尿器外科 28 ( 3 ) 369 - 374 2015.3

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Offspring production with sperm grown in vitro from cryopreserved testis tissues Reviewed

Tetsuhiro Yokonishi, Takuya Sato, Mitsuru Komeya, Kumiko Katagiri, Yoshinobu Kubota, Kazuhiko Nakabayashi, Kenichiro Hata, Kimiko Inoue, Narumi Ogonuki, Atsuo Ogura, Takehiko Ogawa

NATURE COMMUNICATIONS 5 4320 2014.7

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DOI： 10.1038/ncomms5320

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【生殖再生医学】精子幹細胞

古目谷暢, 小川毅彦

HORMONE FRONTIER IN GYNECOLOGY 21 ( 2 ) 131 - 136 2014.6

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新生仔マウス精巣組織の凍結保存とin vitro精子形成

横西哲広, 佐藤卓也, 古目谷暢, 片桐久美子, 越後貫成美, 井上貴美子, 小倉淳郎, 秦健一郎, 窪田吉信, 小川毅彦

日本泌尿器科学会総会 102回 472 - 472 2014.4

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Evaluation and validation of the Core Lower Urinary Tract Symptom Score as an outcome assessment tool for the treatment of benign prostatic hyperplasia: Effects of the alpha 1-adrenoreceptor antagonist silodosin Reviewed

Hiroki Ito, Futoshi Sano, Takehiko Ogawa, Masahiro Yao

INTERNATIONAL JOURNAL OF UROLOGY 21 ( 1 ) 108 - 112 2014.1

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DOI： 10.1111/iju.12167

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In vitro sperm production from mouse spermatogonial stem cell lines using an organ culture method Reviewed

Takuya Sato, Kumiko Katagiri, Yoshinobu Kubota, Takehiko Ogawa

NATURE PROTOCOLS 8 ( 11 ) 2098 - 2104 2013.11

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DOI： 10.1038/nprot.2013.138

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新生仔マウス精巣組織の凍結保存とin vitro精子形成

横西哲広, 古目谷暢, 佐藤卓也, 片桐久美子, 越後貫成美, 井上貴美子, 小倉淳郎, 窪田吉信, 小川毅彦

日本生殖医学会雑誌 58 ( 4 ) 319 - 319 2013.10

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新生仔マウス精巣組織の凍結保存とin vitro精子形成

横西哲広, 佐藤卓也, 古目谷暢, 片桐久美子, 窪田吉信, 井上貴美子, 越後貫成美, 小倉淳郎, 小川毅彦

日本癌治療学会誌 48 ( 3 ) 1480 - 1480 2013.9

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In Vitro Reconstruction of Mouse Seminiferous Tubules Supporting Germ Cell Differentiation Reviewed

Tetsuhiro Yokonishi, Takuya Sato, Kumiko Katagiri, Mitsuru Komeya, Yoshinobu Kubota, Takehiko Ogawa

BIOLOGY OF REPRODUCTION 89 ( 1 ) 2013.7

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DOI： 10.1095/biolreprod.113.108613

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新生仔マウス精巣組織の凍結保存とin vitro精子形成

横西哲広, 古目谷暢, 佐藤卓也, 片桐久美子, 越後貫成美, 井上貴美子, 小倉淳郎, 窪田吉信, 小川毅彦

日本小児泌尿器科学会雑誌 22 ( 2 ) 214 - 214 2013.6

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Evaluation of Preoperative Measurement of Stone Surface Area as a Predictor of Stone-Free Status After Combined Ureteroscopy with Holmium Laser Lithotripsy: A Single-Center Experience Reviewed

Hiroki Ito, Takashi Kawahara, Hideyuki Terao, Takehiko Ogawa, Masahiro Yao, Yoshinobu Kubota, Junichi Matsuzaki

JOURNAL OF ENDOUROLOGY 27 ( 6 ) 715 - 721 2013.6

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DOI： 10.1089/end.2012.0548

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マウス未熟精巣を用いたin vitroにおける精細管再構築と精子形成

横西哲広, 片桐久美子, 古目谷暢, 佐藤卓也, 窪田吉信, 小川毅彦

日本泌尿器科学会雑誌 104 ( 2 ) 222 - 222 2013.3

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In vitro spermatogenesis using an organ culture technique. Reviewed International journal

Tetsuhiro Yokonishi, Takuya Sato, Kumiko Katagiri, Takehiko Ogawa

Methods in molecular biology (Clifton, N.J.) 927 479 - 88 2013

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Research on in vitro spermatogenesis has a long history and remained to be an unaccomplished task until very recently. In 2010, we succeeded in producing murine sperm from primitive spermatogonia using an organ culture method. The fertility of the sperm or haploid spermatids was demonstrated by microinsemination. This organ culture technique uses the classical air-liquid interphase method and is based on conditions extensively examined by Steinbergers in 1960s. Among adaptations in the new culture system, application of serum-free media was the most important. The system is very simple and easy to follow.

DOI： 10.1007/978-1-62703-038-0_41

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Regeneration of Spermatogenesis after Testicular Cancer Chemotherapy Reviewed

Kotaro Suzuki, Yasushi Yumura, Takehiko Ogawa, Kazuo Saito, Yuzo Kinoshita, Kazunii Noguchi

UROLOGIA INTERNATIONALIS 91 ( 4 ) 445 - 450 2013

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DOI： 10.1159/000351189

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Correlation between the operation time using two different power settings of a Ho: YAG laser: Laser power doesn't influence lithotripsy time Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Yoshitake Kato, Katsuyuki Tanaka, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

BMC Research Notes 6 ( 1 ) 80 2013

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DOI： 10.1186/1756-0500-6-80

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An in vitro Method for Producing Sperm for ICSI

Ogawa Takehiko

Journal of Mammalian Ova Research 30 ( 4 ) 155 - 158 2013

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It is a challenge to induce spermatogenesis in vitro, because it involves a complicated process of sequential cell proliferation and differentiation, from spermatogonial stem cells to sperm formation. Recently, we have succeeded, using a classical organ culture method, to produce sperm from spermatogonial stem cells, using several modifications. The produced spermatids and sperm were fertile, giving rise to healthy progeny. This new in vitro system for spermatogenesis could be useful for addressing the issue of sperm quality that is becoming more important in this ICSI era. We also hope that an in vitro system of human spermatogenesis will be developed in the near future. 

DOI： 10.1274/jmor.30.155

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Utility and limitation of cumulative stone diameter in predicting urinary stone burden at flexible ureteroscopy with holmium laser lithotripsy: a single-center experience. Reviewed

Ito H, Kawahara T, Terao H, Ogawa T, Yao M, Kubota Y, Matsuzaki J

PloS one 8 ( 6 ) e65060 2013

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Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse Reviewed

Shiura H, Ikeda R, Lee J, Sato T, Ogonuki N, Hirose M, Ogura A, Ogawa T, Abe K

Genesis 51 ( 7 ) 498 - 505 2013

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DOI： 10.1002/dvg.22391

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The Arf GAP SMAP2 is necessary for organized vesicle budding from the Trans-Golgi-network and subsequent acrosome formation in spermiogenesis. Reviewed

WATANABE Toshio, Tomo Funaki, Shunsuke Kon, Kenji Tanabe, Waka Natsume, Sayaka Sato, Tadafumi Shimizu, Naomi Yoshida, Won Fen Wonga, Atsuo Ogura, Takehiko Ogawa, Kimiko Inoue, Narumi Ogonuki, Hiromi Miki, Keiji Mochida, Keisuke Endoh, Kentarou Yomogida, Manabu Fukumoto, Reiko Horai, Yoichiro Iwakura, Chizuru Ito, Kiyotaka Toshimori, Toshio Watanabe, Masanobu Satake

Molecular Biology of the Cell 123 1123 - 1137 2013

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DOI： 10.1091/mbc.E13-05-0234

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Ureteroscopy-assisted retrograde nephrostomy (UARN) for an incomplete double ureter Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Yoshitake Kato, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

UROLOGICAL RESEARCH 40 ( 6 ) 781 - 782 2012.12

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DOI： 10.1007/s00240-012-0486-y

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Changing to a loop-type ureteral stent decreases patients' stent-related symptoms Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

UROLOGICAL RESEARCH 40 ( 6 ) 763 - 767 2012.12

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DOI： 10.1007/s00240-012-0500-4

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Amplatz Sheath for Cystolithotripsy Using Ho: YAG Laser in Female Patients Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Yoshitake Kato, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

UROLOGY 80 ( 5 ) 1154 - 1155 2012.11

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DOI： 10.1016/j.urology.2012.07.046

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Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice Reviewed

Takuya Sato, Tetsuhiro Yokonishi, Mitsuru Komeya, Kumiko Katagiri, Yoshinobu Kubota, Shogo Matoba, Narumi Ogonuki, Atsuo Ogura, Shosei Yoshida, Takehiko Ogawa

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109 ( 42 ) 16934 - 16938 2012.10

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DOI： 10.1073/pnas.1211845109

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Predictive Value of Attenuation Coefficients Measured as Hounsfield Units on Noncontrast Computed Tomography During Flexible Ureteroscopy with Holmium Laser Lithotripsy: A Single-Center Experience Reviewed

Hiroki Ito, Takashi Kawahara, Hideyuki Terao, Takehiko Ogawa, Masahiro Yao, Yoshinobu Kubota, Junichi Matsuzaki

JOURNAL OF ENDOUROLOGY 26 ( 9 ) 1125 - 1130 2012.9

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DOI： 10.1089/end.2012.0154

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Ureteroscopy assisted retrograde nephrostomy for complete staghorn renal calculi Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

Current Urology 6 ( 2 ) 102 - 105 2012.9

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DOI： 10.1159/000343519

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The Most Reliable Preoperative Assessment of Renal Stone Burden as a Predictor of Stone-free Status After Flexible Ureteroscopy With Holmium Laser Lithotripsy: A Single-center Experience Reviewed

Hiroki Ito, Takashi Kawahara, Hideyuki Terao, Takehiko Ogawa, Masahiro Yao, Yoshinobu Kubota, Junichi Matsuzaki

UROLOGY 80 ( 3 ) 524 - 528 2012.9

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DOI： 10.1016/j.urology.2012.04.001

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Preoperative stenting for ureteroscopic lithotripsy for a large renal stone Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Hanako Ishigaki, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

INTERNATIONAL JOURNAL OF UROLOGY 19 ( 9 ) 881 - 885 2012.9

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DOI： 10.1111/j.1442-2042.2012.03046.x

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Ureteroscopy assisted retrograde nephrostomy: a new technique for percutaneous nephrolithotomy (PCNL) Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Minoru Yoshida, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

BJU INTERNATIONAL 110 ( 4 ) 588 - 590 2012.8

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DOI： 10.1111/j.1464-410X.2011.10795.x

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Stone area and volume are correlated with operative time for cystolithotripsy for bladder calculi using a holmium: yttrium garnet laser Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

SCANDINAVIAN JOURNAL OF UROLOGY AND NEPHROLOGY 46 ( 4 ) 298 - 303 2012.8

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DOI： 10.3109/00365599.2012.672456

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Ureteroscopy-assisted retrograde nephrostomy for percutaneous nephrolithotomy after urinary diversion. Reviewed

Kawahara T, Ito H, Terao H, Ogawa T, Uemura H, Kubota Y, Matsuzaki J

Case reports in nephrology and urology 2 ( 2 ) 113 - 117 2012.7

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DOI： 10.1159/000342338

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Which is the best method to estimate the actual ureteral length in patients undergoing ureteral stent placement? Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Minoru Yoshida, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

INTERNATIONAL JOURNAL OF UROLOGY 19 ( 7 ) 634 - 638 2012.7

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DOI： 10.1111/j.1442-2042.2012.02998.x

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The 7th American Urological Association and the Japanese Urological Association international affiliate society meeting. Reviewed International journal

Homma Y, Kakizaki H, Smith JA Jr, Namiki S, Arai Y, Tomita Y, Uzzo R, Tsuchiya N, Takahashi M, Ichikawa T, Quek ML, Uemura H, Mizokami A, Kakizaki H, Steers WD, Gotoh M, Ogawa T, Chancellor MB, Yamamoto S, Takahashi S, Ichihara K

International journal of urology : official journal of the Japanese Urological Association 19 ( 4 ) 374 - 385 2012.4

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DOI： 10.1111/j.1442-2042.2012.02984.x

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In vitro spermatogenesis: The dawn of a new era in the study of male infertility Reviewed

Takehiko Ogawa

INTERNATIONAL JOURNAL OF UROLOGY 19 ( 4 ) 282 - 283 2012.4

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DOI： 10.1111/j.1442-2042.2012.02979.x

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Ureteral Stent Retrieval Using the Crochet Hook Technique in Females Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Takuya Yamagishi, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

PLOS ONE 7 ( 1 ) e29292 2012.1

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DOI： 10.1371/journal.pone.0029292

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[Three dysuria adults without continuous medical treatment after surgery for spina bifida in infancy]. Reviewed

Tetsuhiro Yokonishi, Atsushi Fujikawa, Katsuyuki Tanaka, Hironobu Sato, Kazuhiko Tokoro, Tomokazu Takakura, Takehiko Ogawa

Hinyokika kiyo. Acta urologica Japonica 58 ( 1 ) 31 - 4 2012.1

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We encountered three patients with dysuria who had undergone spinal surgery for spina bifida during infancy. The patients presented with mental disability and dysbasia. Difficulty in urination, urinary incontinence, and a residual sensation of urine were resolved through intermittent self-catheterization in all patients. It was speculated that treatment for dysuria in these patients was delayed because they were not aware of its association with their condition during infancy, dysuria was relatively mild, and they had no history of febrile urinary tract infection. It is important for attending physicians to explain to parents of such infants the possible association of spina bifida with the future risk of dysuria, and to consider long-term follow-up to monitor their outcome.

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Choosing an Appropriate Length of Loop Type Ureteral Stent Using Direct Ureteral Length Measurement Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Minoru Yoshida, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

UROLOGIA INTERNATIONALIS 88 ( 1 ) 48 - 53 2012

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DOI： 10.1159/000332431

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Encrusted ureteral stent retrieval using flexible ureteroscopy with a Ho: YAG laser Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

Case Reports in Medicine 2012 862539 2012

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Language：English Publishing type：Research paper (scientific journal) Publisher：Hindawi Limited

DOI： 10.1155/2012/862539

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Ureteroscopy-assisted retrograde nephrostomy (UARN) after Anatrophic Nephrolithotomy Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Yoshitake Kato, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

Case Reports in Medicine 2012 164963 2012

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Language：English Publishing type：Research paper (scientific journal) Publisher：Hindawi Limited

DOI： 10.1155/2012/164963

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Ureteroscopy-assisted retrograde nephrostomy for lower calyx calculi in horseshoe kidney: Two case reports Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Katsuyuki Tanaka, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

Journal of Medical Case Reports 6 194 2012

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DOI： 10.1186/1752-1947-6-194

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Ureteral Stent Exchange under Fluoroscopic Guidance Using the Crochet Hook Technique in Women Reviewed

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Yuzo Yamashita, Katsuyuki Tanaka, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

UROLOGIA INTERNATIONALIS 88 ( 3 ) 322 - 325 2012

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DOI： 10.1159/000336870

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In vitro production of fertile sperm from murine spermatogonial stem cell lines Reviewed

Takuya Sato, Kumiko Katagiri, Tetsuhiro Yokonishi, Yoshinobu Kubota, Kimiko Inoue, Narumi Ogonuki, Shogo Matoba, Atsuo Ogura, Takehiko Ogawa

NATURE COMMUNICATIONS 2 472 2011.9

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DOI： 10.1038/ncomms1478

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In Vitro Production of Functional Sperm in Cultured Neonatal Mouse Testes Reviewed

Takuya Sato, Kumiko Katagiri, Kimiko Inoue, Narumi Ogonuki, Atsuo Ogura, Yoshinobu Kubota, Takehiko Ogawa

BIOLOGY OF REPRODUCTION 85 2011.7

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DOI： 10.1095/biolreprod.110.089342

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In vitro production of functional sperm in cultured neonatal mouse testes Reviewed

Takuya Sato, Kumiko Katagiri, Ayako Gohbara, Kimiko Inoue, Narumi Ogonuki, Atsuo Ogura, Yoshinobu Kubota, Takehiko Ogawa

NATURE 471 ( 7339 ) 504 - + 2011.3

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1038/nature09850

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APP-071 器官培養法を用いた新生仔マウス精巣でのin vitro精子産生(総会賞応募ポスター,第99回日本泌尿器科学会総会)

小川毅彦, 佐藤卓也, 横西哲弘, 郷原絢子, 河路かおる, 窪田吉信

日本泌尿器科学会雑誌 102 ( 2 ) 328 - 328 2011

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Language：Japanese Publisher：一般社団法人日本泌尿器科学会

DOI： 10.5980/jpnjurol.102.328_1

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PP-157 TFS(Tissue Fixation System)を用いた腹圧性尿失禁および骨盤臓器脱の治療についての検討(発表・討論,一般演題ポスター,第99回日本泌尿器科学会総会)

河路かおる, 関口由紀, 坂田綾子, 槙山和秀, 中井川昇, 上村博司, 小川毅彦, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 102 ( 2 ) 429 - 429 2011

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DOI： 10.5980/jpnjurol.102.429_5

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Proliferation of Mouse Spermatogonial Stem Cells in Microdrop Culture

Yasuyuki Araki, Takuya Sato, Kumiko Katagiri, Yoshinobu Kubota, Yasuhisa Araki, Takehiko Ogawa

BIOLOGY OF REPRODUCTION 83 ( 6 ) 951 - 957 2010.12

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1095/biolreprod.109.082800

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陳旧性結核腎に伴う腎細胞癌の1例

古目谷暢, 佐野太, 村上貴之, 槇山和秀, 三好康秀, 中井川昇, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

泌尿器外科 23 ( 11 ) 1656 - 1656 2010.11

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In Vitro Murine Spermatogenesis in an Organ Culture System Reviewed

Ayako Gohbara, Kumiko Katagiri, Takuya Sato, Yoshinobu Kubota, Hiroyuki Kagechika, Yasuyuki Araki, Yasuhisa Araki, Takehiko Ogawa

BIOLOGY OF REPRODUCTION 83 ( 2 ) 261 - 267 2010.8

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DOI： 10.1095/biolreprod.110.083899

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Transurethral Bladder Tumor Resection (TUR-Bt) in a Patient With Osler-Rendu-Weber Syndrome Reviewed

Takashi Kawahara, Zenkichi Sekiguchi, Kaoru Kita, Kazuhide Makiyama, Noboru Nakaigawa, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota

UROLOGY 75 ( 6 ) 1518 - 1518 2010.6

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DOI： 10.1016/j.urology.2010.02.034

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前立腺癌MAB療法中に発症した前立腺肉腫に対するIMRT治療を行った1例

河原崇司, 上村博司, 南本亮吾, 山中正二, 関口善吉, 喜多かおる, 槙山和秀, 中井川昇, 小川毅彦, 矢尾正祐, 井上登美夫, 窪田吉信

泌尿器外科 23 ( 臨増 ) 521 - 521 2010.3

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Chemotherapy with low-dose docetaxel and estramustine phosphate in patient with liver dysfunction due to liver metastases of hormone-refractory prostate cancer: A case report Reviewed

Miki Miyake, Noboru Nakaigawa, Kaoru Kita, Masahiro Yanagisawa, Hideyuki Terao, Futoshi Sano, Takayuki Murakami, Kazuhide Makiyama, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota

Acta Urologica Japonica 56 ( 1 ) 45 - 48 2010.1

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Gallbladder metastasis from renal cell carcinoma Reviewed

Takashi Kawahara, Hisashi Ohshiro, Zenkichi Sekiguchi, Mitsuko Furuya, Kazuhiro Namura, Hiroki Itoh, Futoshi Sano, Kaoru Kawaji, Narihiko Hayashi, Kazuhide Makiyama, Noboru Nakaigawa, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota

Case Reports in Oncology 3 ( 1 ) 30 - 34 2010

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DOI： 10.1159/000279308

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PP-247 根治的前立腺全摘術後尿失禁に対する干渉低周波治療についての検討(発表・討論,一般演題ポスター,第98回日本泌尿器科学会総会)

南村和宏, 上村博司, 喜多かおる, 関口善吉, 河原崇司, 伊藤悠城, 佐野太, 林成彦, 槙山和秀, 中井川昇, 小川毅彦, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 101 ( 2 ) 430 - 430 2010

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DOI： 10.5980/jpnjurol.101.430_3

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PP-602 進行性精巣腫瘍に対する導入化学療法抵抗例の検討(発表・討論,一般演題ポスター,第98回日本泌尿器科学総会)

岸田健, 滝沢明利, 太田淳一, 平井耕太郎, 杉浦晋平, 塩井康一, 石垣華子, 寺西淳一, 三好康秀, 野口和美, 小川毅彦, 窪田吉信, 三浦猛

日本泌尿器科学会雑誌 101 ( 2 ) 519 - 519 2010

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DOI： 10.5980/jpnjurol.101.519_2

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PP-463 マウス培養精原細胞のマイクロドロップ培養下での増殖動態(発表・討論,第98回日本泌尿器科学会総会)

小川毅彦, 荒木泰行, 荒木康久, 郷原絢子, 佐藤卓也, 窪田吉信

日本泌尿器科学会雑誌 101 ( 2 ) 484 - 484 2010

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DOI： 10.5980/jpnjurol.101.484_3

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Neuroendocrine carcinoma of the bladder Reviewed

Takashi Kawahara, Shoji Yamanaka, Hisashi Ohshiro, Zenkichi Sekiguchi, Kazuhiro Namura, Hiroki Itou, Futoshi Sano, Kaoru Kita, Narihiko Hayashi, Kazuhide Makiyama, Noboru Nakaigawa, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota

Case Reports in Oncology 3 ( 1 ) 54 - 58 2010

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DOI： 10.1159/000289584

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Primary Synovial Sarcoma of the Kidney. Reviewed

Kawahara T, Sekiguchi Z, Makiyama K, Nakayama T, Nagashima Y, Kita K, Namura K, Itou H, Sano F, Hayashi N, Nakaigawa N, Ogawa T, Uemura H, Yao M, Kubota Y

Case reports in oncology 2 ( 3 ) 189 - 193 2009.10

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Laparoscopic right partial nephrectomy in the presence of riedel's lobe of the liver Reviewed

Miki Miyaké, Kazuhide Makiyama, Noboru Nakaigawa, Yasuhide Miyoshi, Futoshi Sano, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota

Acta Urologica Japonica 55 ( 8 ) 509 - 511 2009.8

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Mixed epithelial and stromal tumor of kidney: A case report Reviewed

Hideyuki Terao, Kazuhide Makiyama, Masahiro Yanagisawa, Miki Miyake, Futoshi Sano, Kaoru Kita, Takayuki Murakami, Noboru Nakaigawa, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota, Yoshiaki Inayama, Yoii Nagashima

Acta Urologica Japonica 55 ( 8 ) 495 - 498 2009.8

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18F-FDG PET-CTによる前立腺がんの診断

河原崇司, 上村博司, 寺尾秀行, 佐野太, 村上貴之, 槙山和秀, 中井川昇, 小川毅彦, 矢尾正祐, 南本亮吾, 井上登美夫, 窪田吉信

泌尿器外科 22 ( 8 ) 1057 - 1059 2009.8

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A case of adult-onset idiopathic hypogonadotropic hypogonadism presenting with infertility Reviewed

Hideyuki Terao, Takehiko Ogawa, Masahiro Yanagisawa, Miki Miyake, Futoshi Sano, Kaoru Kita, Takayuki Murakami, Kazuhide Makiyama, Noboru Nakaigawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota

Acta Urologica Japonica 55 ( 7 ) 437 - 439 2009.7

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A case of upper urinary tract metastases from sigmoid colon cancer Reviewed

Mitsuru Komeya, Noboru Nakaigawa, Futoshi Sano, Masayo Kagota, Takayuki Murakami, Kazuhide Makiyama, Yasuhide Miyoshi, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoji Nagashima, Yoshinobu Kubota

Acta Urologica Japonica 55 ( 6 ) 339 - 343 2009.6

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A case of sarcomatoid renal cell carcinoma developed in the chalked kidney (Putty Kidney) Reviewed

Mitsuru Komeya, Futoshi Sano, Masayo Kagota, Takayuki Murakami, Kazuhide Makiyama, Yasuhide Miyoshi, Noboru Miyoshi, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Hisashi Ooshiro, Yoji Nagashima, Yoshinobu Kubota

Acta Urologica Japonica 55 ( 5 ) 253 - 257 2009.5

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Ectopic porcine spermatogenesis in murine subcutis: tissue grafting versus cell-injection methods Reviewed

Takeshi Watanabe, Hirofumi Hayashi, Kaoru Kita, Yoshinobu Kubota, Takehiko Ogawa

ASIAN JOURNAL OF ANDROLOGY 11 ( 3 ) 317 - 323 2009.4

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DOI： 10.1038/aja.2008.5

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Effect of reactive oxygen species in semen on the pregnancy of infertile couples Reviewed

Yasushi Yumura, Akira Iwasaki, Kazuo Saito, Takehiko Ogawa, Makoto Hirokawa

INTERNATIONAL JOURNAL OF UROLOGY 16 ( 2 ) 202 - 207 2009.2

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DOI： 10.1111/j.1442-2042.2008.02213.x

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PP-006 当院での表在性膀胱癌のTUR-Bt後再発率に関しての検討(膀胱腫瘍/手術1,一般演題ポスター,第97回日本泌尿器科学会総会)

寺尾秀行, 柳澤昌宏, 三宅見季, 佐野太, 喜多かおる, 村上貴之, 槙山和秀, 中井川昇, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 100 ( 2 ) 318 - 318 2009

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DOI： 10.5980/jpnjurol.100.318_2

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PP-455 精巣腫瘍に対する化学療法の妊孕性への影響(不妊1,一般演題ポスター,第97回日本泌尿器科学会総会)

鈴木康太郎, 斎藤和男, 服部裕介, 湯村寧, 野口和美, 岩崎晧, 小川毅彦, 池田伊知郎, 窪田吉信

日本泌尿器科学会雑誌 100 ( 2 ) 430 - 430 2009

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DOI： 10.5980/jpnjurol.100.430_3

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PP-601 前立腺生検と前立腺全摘標本における一般病理医のGleason scoreと泌尿器病理医のGleason scoreとの比較(前立腺腫瘍/診断・マーカー1,一般演題ポスター,第97回日本泌尿器科学会総会)

三好康秀, 上村博司, 村上貴之, 槙山和秀, 中井川昇, 小川毅彦, 矢尾正祐, 窪田吉信, 稲山嘉明, 佐々木毅, 野口和美

日本泌尿器科学会雑誌 100 ( 2 ) 467 - 467 2009

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DOI： 10.5980/jpnjurol.100.467_1

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OP-360 経直腸的超音波検査における第2世代超音波造影剤(ソナゾイド)の前立腺癌描出能に関する検討(第2報)(前立腺腫瘍/診断・マーカー4,一般演題口演,第97回日本泌尿器科学会総会)

佐野太, 上村博司, 寺尾秀行, 佐々木毅, 三宅見季, 柳澤昌宏, 喜多かおる, 村上貴之, 槙山和秀, 中井川昇, 小川毅彦, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 100 ( 2 ) 278 - 278 2009

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DOI： 10.5980/jpnjurol.100.278_4

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Progress of In Vitro Culture Systems of Spermatogonia

Ohbo Kazuyuki, Ogawa Takehiko

Journal of Mammalian Ova Research 26 ( 4 ) 178 - 182 2009

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Mammalian spermatogenesis is a classic adult stem cell system. Identification of a crucial self renewal factor, glial cell line derived neurotrophic factor (GDNF), has provided new opportunities in cell culture systems of testicular cells. Spermatogonia can be maintained for years in culture with GDNF. Transplantation experiments of cultured spermatogonia have shown that they are able to reconstitute all the germ cells with proper programming in the testes, suggesting that the stem cell population of spermatogonia is maintained in vitro for at least, a couple years. Recently, it has also been reported that cultured spermatogonia have the potency to convert to multi-potent stem cells. The rapid progress of in vitro culture systems of spermatogonia makes it possible to apply the culture systems not only to the study of male infertility but also to regenerative medicine. 

DOI： 10.1274/jmor.26.178

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PP-150 進行癌・再発癌における経皮的腎ろう造設術についての検討(上部尿路/機能,一般演題ポスター,第97回日本泌尿器科学会総会)

喜多かおる, 柳澤昌宏, 三宅見季, 寺尾秀行, 佐野太, 村上貴之, 槙山和秀, 中井川昇, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 100 ( 2 ) 354 - 354 2009

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DOI： 10.5980/jpnjurol.100.354_2

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Free PSA/Total PSA Ratio Increases the Detection Rate of Prostate Cancer in Twelve-Core Biopsy Reviewed

Yumiko Yokomizo, Yasuhide Miyoshi, Noboru Nakaigawa, Kazuhide Makiyama, Takehiko Ogawa, Masahiro Yao, Yoshinobu Kubota, Hiroji Uemura

UROLOGIA INTERNATIONALIS 82 ( 3 ) 280 - 285 2009

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DOI： 10.1159/000209358

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PP-636 当院における内視鏡補助下小切開前立腺全摘術の手術成績に影響をおよぼす因子についての検討(前立腺腫瘍/ミニマム創手術・HIFU,一般演題ポスター,第97回日本泌尿器科学会総会)

柳澤昌宏, 三宅見季, 寺尾秀行, 佐野太, 喜多かおる, 村上貴之, 槙山和秀, 三好康秀, 中井川昇, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 100 ( 2 ) 475 - 475 2009

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DOI： 10.5980/jpnjurol.100.475_4

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PP-460 マウス精巣組織器官培養を用いたin vitro精子形成に影響する諸因子の検討(不妊2,一般演題ポスター,第97回日本泌尿器科学会総会)

小川毅彦, 渡辺岳志, 喜多かおる, 服部裕介, 湯村寧, 窪田吉信

日本泌尿器科学会雑誌 100 ( 2 ) 431 - 431 2009

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DOI： 10.5980/jpnjurol.100.431_4

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OP-214 Stage D2前立腺癌症例に対するホルモン療法とゾレドロン酸併用療法に関する前向き多施設共同臨床試験の中間報告(前立腺腫瘍/薬物療法4,一般演題口演,第97回日本泌尿器科学会総会)

上村博司, 佐野太, 小川毅彦, 矢尾正祐, 寺西淳一, 三好康秀, 梅本晋, 古目屋暢, 岩崎晧, 野口和美, 窪田吉信

日本泌尿器科学会雑誌 100 ( 2 ) 242 - 242 2009

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DOI： 10.5980/jpnjurol.100.242_1

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PP-429 再燃前立腺癌に対するドセタキセル療法の長期投与についての検討(前立腺腫瘍/薬物療法1,一般演題ポスター,第97回日本泌尿器科学会総会)

河原崇司, 上村博司, 寺西淳一, 佐野太, 服部裕介, 村上貴之, 槙山和秀, 三好康秀, 中井川昇, 小川毅彦, 矢尾正祐, 野口和美, 窪田吉信

日本泌尿器科学会雑誌 100 ( 2 ) 424 - 424 2009

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DOI： 10.5980/jpnjurol.100.424_1

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(Selectivity): Silodosin early effectiveness and safety multi center trial in Yokohama multi-center trial on the early effects of silodosin on lower urinary tract symptoms associated with benign prostatic hyperplasia Reviewed

Takehiko Ogawa, Hirqji Uemura, Futoshi Sano, Kouji Hoshino, Kaoru Kita, Narihiko Hayashi, Masayo Kagota, Takayuki Murakami, Kazuhide Makiyama, Yasuhide Miyoshi, Noboru Nakaigawa, Masahiro Yao, Hidetoshi Shimura, Akihiko Furuhata, Naoya Fujikawa, Koichi Shioi, Teiichiro Ueki, Yoshinori Hara, Kiyoshi Saitou, Satoshi Kawakami, Yoshiaki Satomi, Yusuke Hattori, Junichi Teranishi, Keiichi Kondo, Takeshi Kishida, Kazuo Saito, Kazumi Noguchi, Hiroshi Fujii, Toyoaki Yamaguchi, Yuzo Kinoshita, Sakae Nomura, Minoru Yoshida, Naoki Sakai, Hideyuki Terao, Tatsuya Matsumoto, Susumu Umemoto, Yoshiharu Ogo, Kazuki Kobayashi, Sumio Noguchi, Tomoyuki Asakura, Teruo Kohdaira, Kozue Iguchi, Koji Izumi, Masami Hirano, Hitomi Kanno, Toshihiro Takahashi, Fumihiko Nukui, Yasuhiro Mokuo, Kentaro Muraoka, Yutaka Osada, Makoto Funahashi, Kazuo Kitami, Shimpei Sugiura, Junichi Ohta, Takeshi Miura, Yoshio Ishibashi, Mitsunobu Masuda, Atsushi Komiya, Yutaka Suwa, Takafumi Hashiba, Kunihisa Mitaka, Masataka Kobayashi, Kimito Ohsaka, Tetsuzo Takano, Futoshi Tsuchiya, Akira Iwasaki, Yuzo Yamashita, Junichi Matsuzaki, Yoshinobu Kubota

Acta Urologica Japonica 54 ( 12 ) 757 - 764 2008.12

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Differences and similarities in the developmental status of embryo-derived stem cells and by global expression profiling Reviewed

Nathan Mise, Takuya Fuchikami, Michihiko Sugimoto, Satoru Kobayakawa, Fumio Ike, Takehiko Ogawa, Takashi Tada, Shigehiko Kanaya, Toshiaki Noce, Kuniya Abe

GENES TO CELLS 13 ( 8 ) 863 - 877 2008.8

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DOI： 10.1111/j.1365-2443.2008.01211.x

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Identification of transcripts commonly expressed in both hematopoietic and germ-line stem cells Reviewed

Takuo Mizukami, Madoka Kuramitsu, Kazuya Takizawa, Haruka Momose, Atsuko Masumi, Seishiro Naito, Atsushi Iwama, Takehiko Ogawa, Toshiaki Noce, Isao Hamaguchi, Kazunari Yamaguchi

STEM CELLS AND DEVELOPMENT 17 ( 1 ) 67 - 80 2008.2

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DOI： 10.1089/scd.2007.0077

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Reproductive stem cell research and its application to urology

Takehiko Ogawa

INTERNATIONAL JOURNAL OF UROLOGY 15 ( 2 ) 121 - 127 2008.2

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DOI： 10.1111/j.1442-2042.2007.01963.x

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S状結腸癌の腎盂尿管転移の一例

古目谷暢, 増田貴文, 伊藤悠介, 佐野太, 村上貴之, 槙山和秀, 三好康秀, 中井川昇, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

神奈川医学会雑誌 35 ( 1 ) 93 - 94 2008.1

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OP-285 前立腺肥大症に伴う排尿障害に対するシロドシンの服用後早期効果に関する多施設共同臨床研究(SELECTIVITY)(前立腺肥大症/診断・薬物療法2,一般演題口演,第96回日本泌尿器科学会総会)

小川毅彦, 上村博司, 乙矢尾正祐, 古畑哲彦, 酒井直樹, 里見佳昭, 木下裕三, 藤井浩, 志村英俊, 山口豊明, 河上哲, 朝倉智行, 窪田吉信

日本泌尿器科学会雑誌 99 ( 2 ) 343 - 343 2008

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DOI： 10.5980/jpnjurol.99.343_1

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PP-091 アンジオテンシンIIレセプターブロッカーによる前立腺全摘後PSA再発の延長効果(前立腺腫瘍/薬物療法1,一般演題ポスター,第96回日本泌尿器科学会総会)

上村博司, 寺西淳一, 三好康秀, 佐野太, 星野耕二, 村上貴之, 槙山和秀, 中井川昇, 小川毅彦, 矢尾正祐, 野口和美, 窪田吉信

日本泌尿器科学会雑誌 99 ( 2 ) 400 - 400 2008

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DOI： 10.5980/jpnjurol.99.400_3

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精子系細胞の体外分化と成熟

小川毅彦

産婦人科治療 90 ( 4 ) 2008

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[Spermatogenic stem cells and pluripoent stem cells]. Reviewed

Ogawa T

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 52 ( 4 ) 356 - 363 2007.4

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Other Link： http://search.jamas.or.jp/link/ui/2007180306
Clinical investigation of infertile males with chromosomal anomalies Reviewed

Yasushi Yumura, Kazuo Saito, Takehiko Ogawa, Kotaro Suzuki, Kazuhiko Sato, Yoshinobu Kubota, Akira Iwasaki

Acta Urologica Japonica 53 ( 2 ) 87 - 91 2007.2

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Production of functional spermatids from mouse germline stem cells in ectopically reconstituted seminiferous tubules

Kaoru Kita, Takeshi Watanabe, Kimito Ohsaka, Hirofumi Hayashi, Yoshinobu Kubota, Yoji Nagashima, Ichiro Aoki, Hideki Taniguchi, Toshiaki Noce, Kimiko Inoue, Hiromi Miki, Narumi Ogonuki, Hiromitsu Tanaka, Atsuo Ogura, Takehiko Ogawa

BIOLOGY OF REPRODUCTION 76 ( 2 ) 211 - 217 2007.2

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DOI： 10.1095/biolreprod.106.056895

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[Only metastasis to uterine corpus from superficial bladder cancer that of no original recurrence]. Reviewed

Takayuki Murakami, Koji Hoshino, Hisashi Hasumi, Kazuhide Makiyama, Yasuhide Miyoshi, Minoru Yoshida, Noboru Nakaigawa, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota

Hinyokika kiyo. Acta urologica Japonica 53 ( 1 ) 75 - 7 2007.1

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We report a rare case of uterine corpus metastasis from superficial bladder cancer. A 78-year-old female presented with abnormal vaginal bleeding. She received transurethral resection of bladder tumor (TUR-Bt) two years previously, and the pathological findings were transitional cell carcinoma (TCC) grade 3 pT1. Eight courses of BCG instillation were performed postoperatively. There was no recurrence of bladder cancer when vaginal bleeding occurred. Cytology of vaginal discharge was class V, and transitional cell carcinoma suspected. Pathological finding of transvaginal uterine corpus biopsy was TCC. We diagnosed metastases to uterine corpus from bladder cancer.

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PP-093 腎癌における血清Caと腎癌組織中PTHrp発現に関する検討(第95回日本泌尿器科学会総会)

村上貴之, 伊藤悠亮, 井口梢, 佐野太, 槙山和秀, 三好康秀, 中井川昇, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 98 ( 2 ) 420 - 420 2007

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DOI： 10.5980/jpnjurol.98.420_1

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OP-037 後腹膜鏡下根治的腎摘除術 : 小切開を先行させる術式(第95回日本泌尿器科学会総会)

槙山和秀, 中井川昇, 三好康秀, 村上貴之, 林成彦, 佐野太, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 98 ( 2 ) 300 - 300 2007

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DOI： 10.5980/jpnjurol.98.300_1

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PP-060 腎細胞癌に対する既存抗癌剤の抗腫瘍効果に関する検討(第95回日本泌尿器科学会総会)

佐野太, 中井川昇, 村上貴之, 槙山和秀, 三好康秀, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 98 ( 2 ) 411 - 411 2007

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DOI： 10.5980/jpnjurol.98.411_4

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生殖幹細胞（精子幹細胞と多能性幹細胞）

小川毅彦

蛋白質核酸酵素 52 ( 4 ) 2007

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A case of laparoscopic heminephroureterectomy for ureter cancer in a horseshoe kidney Reviewed

Takayuki Murakami, Kazuhide Makiyama, Yasuhide Miyoshi, Yusuke Ito, Kozue Iguchi, Futoshi Sano, Noboru Nakaigawa, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota

Japanese Journal of Urology 98 ( 6 ) 786 - 789 2007

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DOI： 10.5980/jpnjurol1989.98.786

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A pilot study of combination chemotherapy of gemcitabine and nedaplatin for urological cancer

Susumu Umemoto, Yasuhide Miyoshi, Noboru Nakaigawa, Kazuhide Makiyama, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota

Japanese Journal of Clinical Urology 61 ( 11 ) 903 - 908 2007

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Testosterone administration promotes regeneration of chemically impaired spermatogenesis in rats Reviewed

Koichi Udagawa, Takehiko Ogawa, Takeshi Watanabe, Yoichi Tamura, Kaoru Kita, Yoshinobu Kubota

INTERNATIONAL JOURNAL OF UROLOGY 13 ( 8 ) 1103 - 1108 2006.8

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DOI： 10.1111/j.1442-2042.2006.01484.x

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A case of transitional cell carcinoma with squamous differentiation which developed squamous cell carcinoma in situ in the clinical course Reviewed

Shimpei Sugiura, Kazuhide Makiyama, Yumiko Yokomizo, Susumu Umemoto, Yasuhide Miyoshi, Noboru Nakaigawa, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota, Shoji Yamanaka

Acta Urologica Japonica 52 ( 9 ) 715 - 718 2006.8

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尿管瘤嵌頓の1例 Reviewed

星野耕二, 蓮見壽史, 村上貴之, 槙山和秀, 吉田実, 三好康秀, 中井川昇, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

神奈川医学会雑誌 33 ( 2 ) 231 - 231 2006.7

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[Micropenis]. Reviewed

Ogawa T

Nihon rinsho. Japanese journal of clinical medicine Suppl 2 622 - 627 2006.6

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Propiverine hydrochloride improved correlatively subjective QOL and objective findings in Japanese patients with urinary frequency and/or incontinence Reviewed

Kazumi Noguchi, Takuya Yamagishi, Kotaro Suzuki, Keiichi Kondo, Takeshi Kishida, Kazuo Saito, Yuki Sekiguchi, Noboru Nakaigawa, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota, Masaki Kawai, Kazuto Okajima, Kazuki Kobayashi, Masatoshi Moriyama, Yuzo Kinoshita, Kunihisa Mikata, Kazuo Kitami, Mitsunobu Masuda, Ryuichi Saito, Tetsuo Murai, Takeshi Tomoda, Sumio Noguchi, Naoki Sakai, Atsushi Hamano, Sakae Nomura, Kazuhiko Sato

Acta Urologica Japonica 52 ( 5 ) 343 - 348 2006.5

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A case of salvage combination chemotherapy of gemcitabine plus nedaplatin for squamous cell carcinoma of the ureter Reviewed

Susumu Umemoto, Yasuhide Miyoshi, Yumiko Yokomizo, Shinpei Sugiura, Kazuhide Makiyama, Noboru Nakaigawa, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota

Acta Urologica Japonica 52 ( 1 ) 35 - 39 2006.1

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MP-705 泌尿器癌に対するgemcitabine,nedaplatin併用化学療法の検討(一般演題ポスター,第94回日本泌尿器科学会総会)

梅本晋, 三好康秀, 横溝由美子, 杉浦晋平, 槙山和秀, 中井川昇, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 97 ( 2 ) 543 - 543 2006

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DOI： 10.5980/jpnjurol.97.543_1

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The niche for spermatogonial stem cells in the mammalian testis Reviewed

T Ogawa, M Ohmura, K Ohbo

INTERNATIONAL JOURNAL OF HEMATOLOGY 82 ( 5 ) 381 - 388 2005.12

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DOI： 10.1532/IJH97.05088

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Treatment of androgen-independent, hormone-refractory prostate cancer with docetaxel in Japanese patients. Reviewed

Yasuhide Miyoshi, Hiroji Uemura, Masafumi Nakamura, Hisashi Hasumi, Shinpei Sugiura, Kazuhide Makiyama, Noboru Nakaigawa, Takeshi Kishida, Takehiko Ogawa, Masahiro Yao, Yoshinobu Kubota

International journal of clinical oncology 10 ( 3 ) 182 - 6 2005.6

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BACKGROUND: Although patients with prostate cancer with metastatic lesions initially respond to androgen ablation therapy, most patients ultimately develop a hormone-refractory state. Effective treatment for men with hormone-refractory prostate cancer (HRPC) has not been established. We performed a clinical study of docetaxel in HRPC patients, and evaluated its efficacy. METHODS: Nine patients with HRPC were administered 55 mg/m2 docetaxel, every 3 weeks, simultaneously with hormonal therapy, with a luteinizing hormone-releasing hormone analog, and daily oral dexamethasone. Change in serum prostate-specific antigen (PSA) was determined as the primary endpoint. RESULTS: The mean age of the patients was 64 years (range, 49 to 76 years). Median follow-up time was 8.5 months (range, 5.3 to 16.7 months). In eight patients whose pretreatment serum PSA was elevated, six patients (75.0%) had a PSA decline of more than 50%, and four (50.0%) had a PSA decline of more than 75%. Median time to progression for all patients was 7.9 months (range, 0.0 to 11.6 months; 95% confidence interval [CI], 0.0 to 26.3). The median overall survival was 8.5 months (range, 5.3 to 16.7 months; 95% CI, 8.1 to 13.8). Four of six patients (66.7%) with pain before treatment obtained pain relief and were able to discontinue analgesic agents. This regimen was well tolerated. Grade 3 or 4 neutropenia or leukocytopenia without fever was seen in three patients (33.3%). Only one patient required administration of granulocyte-colony stimulating factor because of neutropenia. No other grade 3 or 4 toxicity was observed. CONCLUSION: Docetaxel was an active agent in Japanese HRPC patients, and was well tolerated in this population. To establish its efficacy and safety in Japanese HRPC patients, a large-scale study in Japan is warranted.

DOI： 10.1007/s10147-005-0490-0

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Composite distal nephron-derived renal cell carcinoma with chromophobe and collecting duct carcinomatous elements. Reviewed

Kawano N, Inayama Y, Nakaigawa N, Yao M, Ogawa T, Aoki I, Kitamura H, Nakatani Y, Nagashima Y

Pathology international 55 ( 6 ) 360 - 365 2005.6

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Renal oncocytosis. Reviewed

Nagashima Y, Mitsuya T, Shioi KI, Noguchi S, Kishida T, Hamano A, Ohgo Y, Tsuura Y, Ogawa T, Aoki I, Yao M

Pathology international 55 ( 4 ) 210 - 215 2005.4

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Two cases of testicular tumors with high α-fetoprotein levels: A case report Reviewed

Makoto Funahashi, Futoshi Tuchiya, Kazuhide Makiyama, Shinpei Sugiura, Yasuhide Miyoshi, Takeshi Kishida, Takehiko Ogawa, Hiroji Uemura, Masahiro Yao, Yoshinobu Kubota

Acta Urologica Japonica 51 ( 2 ) 133 - 137 2005.2

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新しい尿失禁手術TOT-MONARC(第93回日本泌尿器科学会総会)

関口由紀, 井上裕美, 斉藤和男, 小川毅彦, 上村博司, 矢尾正裕, 窪田吉信

日本泌尿器科学会雑誌 96 ( 2 ) 133 - 133 2005

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DOI： 10.5980/jpnjurol.96.133_3

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前立腺癌に対するヨード125シード線源を用いた密封小線源治療 (ブラキセラピー) の経験(第93回日本泌尿器科学会総会)

三好康秀, 上村博司, 杉浦晋平, 槙山和秀, 中井川昇, 岸田健, 小川毅彦, 矢尾正祐, 板澤朋子, 荻野伊知郎, 窪田吉信

日本泌尿器科学会雑誌 96 ( 2 ) 242 - 242 2005

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DOI： 10.5980/jpnjurol.96.242_1

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精巣腫瘍に対する抗がん剤投与後の妊孕性について(第93回日本泌尿器科学会総会)

鈴木康太郎, 齋藤和男, 岸田健, 野口和美, 小川毅彦, 湯村寧, 岩崎晧, 佐藤和彦, 菅野ひとみ, 木下裕三, 窪田吉信

日本泌尿器科学会雑誌 96 ( 2 ) 184 - 184 2005

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DOI： 10.5980/jpnjurol.96.184_2

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転移・再発をきたした腫瘍径4cm以下の腎細胞癌についての検討(第93回日本泌尿器科学会総会)

梅本晋, 横溝由美子, 杉浦晋平, 槙山和秀, 三好康秀, 中井川昇, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 96 ( 2 ) 142 - 142 2005

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DOI： 10.5980/jpnjurol.96.142_4

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GS細胞における多分化能の検討(第93回日本泌尿器科学会総会)

喜多かおる, 小川毅彦, 窪田吉信, 千葉哲博, 谷口英樹

日本泌尿器科学会雑誌 96 ( 2 ) 285 - 285 2005

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DOI： 10.5980/jpnjurol.96.285_3

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Abnormal sperm morphology caused by defects in sertoli cells of Cnot7 knockout mice Reviewed

T Ogawa, C Ito, T Nakamura, Y Tamura, T Yamamoto, T Noda, Y Kubota, K Toshimori

ARCHIVES OF HISTOLOGY AND CYTOLOGY 67 ( 4 ) 307 - 314 2004.11

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DOI： 10.1679/aohc.67.307

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Derivation and morphological characterization of mouse spermatogonial stem cell lines Reviewed

T Ogawa, M Ohmura, Y Tamura, K Kita, K Ohbo, T Suda, Y Kubota

ARCHIVES OF HISTOLOGY AND CYTOLOGY 67 ( 4 ) 297 - 306 2004.11

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DOI： 10.1679/aohc.67.297

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Oligo-astheno-teratozoospermia in mice lacking Cnot7, a regulator of retinoid X receptor beta

T Nakamura, R Yao, T Ogawa, T Suzuki, C Ito, N Tsunekawa, K Inoue, R Ajima, T Miyasaka, Y Yoshida, A Ogura, K Toshimori, T Noce, T Yamamoto, T Noda

NATURE GENETICS 36 ( 5 ) 528 - 533 2004.5

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DOI： 10.1038/ng1344

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AVP-012 新しいPosterior IVS (infracoccygeal sacropexy)による仙骨子宮靭帯補強と排尿状態の改善について(総会賞応募(ビデオ))

関口由紀, 井上裕美, 岸田健, 小川毅彦, 上村博司, 矢尾正裕, 窪田吉信

日本泌尿器科学会雑誌 95 ( 2 ) 309 - 309 2004

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DOI： 10.5980/jpnjurol.95.309_4

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Two cases of 46,XX male with chief complaint of infertility Reviewed

Yasushi Yumura, Hitomi Kanno, Takehiko Ogawa, Kazuo Saito, Kauhiko Sato, Yoshinobu Kubota, Akira Iwasaki, Takuto Sawada

Acta Urologica Japonica 49 ( 12 ) 727 - 734 2003.12

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A case of Müllerian duct cyst with improvement of semen findings after bilateral epididymitis following transurethral resection of hemi-verumontanum

Takuto Sawada, Akira Iwasaki, Eiichi Ishizuka, Yasushi Yumura, Hitomi Kanno, Takehiko Ogawa, Kazuo Saito, Kazuhiko Satoh, Yoshinobu Kubota

Japanese Journal of Fertility and Sterility 48 ( 3-4 ) 45 - 49 2003.10

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Increment of murine spermatogonial cell number by gonadotropin-releasing hormone analogue is independent of stem cell factor c-kit signal

M Ohmura, T Ogawa, M Ono, M Dezawa, M Hosaka, Y Kubota, H Sawada

BIOLOGY OF REPRODUCTION 68 ( 6 ) 2304 - 2313 2003.6

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DOI： 10.1095/biolreprod.102.013276

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Identification and characterization of stem cells in prepubertal spermatogenesis in mice Reviewed

K Ohbo, S Yoshida, M Ohmura, O Ohneda, T Ogawa, H Tsuchiya, T Kuwana, J Kehler, K Abe, HR Scholer, T Suda

DEVELOPMENTAL BIOLOGY 258 ( 1 ) 209 - 225 2003.6

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DOI： 10.1016/S0012-1606(03)00111-8

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Sjogren's syndrome with bilateral hydronephrosis caused by pseudolymphoma of bilateral renal pelves: A case report Reviewed

Junichi Teranishi, Takehiko Ogawa, Kazuo Saito, Kazumi Noguchi, Yoshinobu Kubota

Acta Urologica Japonica 49 ( 2 ) 91 - 93 2003.2

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Expansion of murine spermatogonial stem cells through serial transplantation

T Ogawa, M Ohmura, Y Yumura, H Sawada, Y Kubota

BIOLOGY OF REPRODUCTION 68 ( 1 ) 316 - 322 2003.1

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DOI： 10.1095/biolreprod.102.004549

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癌患者に対する精子凍結保存の意義 : アンケート調査の結果より

齋藤和男, 鈴木康太郎, 野口和美, 小川毅彦, 武田光正, 岩崎晧, 佐藤和彦, 湯村寧, 木下裕三, 窪田吉信

日本泌尿器科学会雑誌 94 ( 2 ) 175 - 175 2003

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DOI： 10.5980/jpnjurol.94.175_1

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精原細胞移植による宿主マウスの妊孕化

小川毅彦, 田村陽一, 喜多かおる, 湯村寧, 平井耕太郎, 武田光正, 窪田吉信

日本泌尿器科学会雑誌 94 ( 2 ) 363 - 363 2003

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DOI： 10.5980/jpnjurol.94.363_4

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Semen cryopreservation for patients with malignant or non-malignant disease: Our experience for 10 years Reviewed

Kazuo Saito, Kotaro Suzuki, Kazumi Noguchi, Takehiko Ogawa, Mitsumasa Takeda, Masahiko Hosaka, Yasushi Yumura, Akira Iwasaki, Kazuhiko Sato, Hitomi Kanno, Yuzo Kinoshita, Yoshinobu Kubota

Japanese Journal of Urology 94 ( 4 ) 513 - 520 2003

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DOI： 10.5980/jpnjurol1989.94.513

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アクティブバースは、尿失禁を増加させるか? : ICIQ-SF(International Consultation on Incontinence Questionnaire-Short Form)による評価

関口由紀, 井上裕美, 後藤百万, 小川毅彦, 平井耕太郎, 喜多かおる, 中井川昇, 岸田健, 上村博司, 武田光正, 矢尾正祐, 馬場理也, 窪田吉信

日本泌尿器科学会雑誌 94 ( 2 ) 173 - 173 2003

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DOI： 10.5980/jpnjurol.94.173_1

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TUR-P術後排尿機能予測における排尿時膀胱尿道造影の可能性

平井耕太郎, 中村昌史, 河合正紀, 馬場理也, 三好康秀, 中井川昇, 岸田健, 上村博司, 関口由紀, 小川毅彦, 矢尾正祐, 武田光正, 窪田吉信

日本泌尿器科学会雑誌 94 ( 2 ) 269 - 269 2003

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DOI： 10.5980/jpnjurol.94.269_2

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Recovery of spermatogenesis by high dose gonadotropinreleasing hormone analogue treatment in rat cryptorchid testis after orchiopexy Reviewed

K Udagawa, M Takeda, M Hosaka, Y Kubota, T Ogawa

JOURNAL OF UROLOGY 168 ( 3 ) 1279 - 1283 2002.9

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DOI： 10.1097/01.ju.0000024626.25898.0f

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The effect of high concentration of sodium and potassium ion on motility of human sperm preserved in electrolyte-free solution Reviewed

Yasushi Yumura, Kazuo Saito, Kotaro Suzuki, Takehiko Ogawa, Hitomi Kanno, Kazuhiko Sato, Akira Iwasaki, Masahiko Hosaka

Japanese Journal of Urology 93 ( 3 ) 440 - 443 2002

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DOI： 10.5980/jpnjurol1989.93.440

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GnRH analogueの精原細胞増殖に対する影響

小川毅彦, 大村昌子, 澤田元, 宇田川幸一, 湯村寧, 窪田吉信

日本泌尿器科学会雑誌 93 ( 2 ) 204 - 204 2002

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DOI： 10.5980/jpnjurol.93.204_1

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Recovery of Spermatogenesis by High Dase Gonadotropin-releasing Hormone Analogue Treatment in Rat Cryptorchid Testis After Orchiopexy.

小川毅彦

J Urol 168/3, 1279-1283 2002

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抗癌剤による精巣傷害後のテストステロン大量投与による精子形成再生の効果

宇田川幸一, 小川毅彦, 渡辺岳志, 湯村寧, 武田光正, 窪田吉信

日本泌尿器科学会雑誌 93 ( 2 ) 206 - 206 2002

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DOI： 10.5980/jpnjurol.93.206_2

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GnRH analog, leuprorelin acetate, promotes regeneration of rat spermatogenesis after severe chemical damage

K Udagawa, T Ogawa, T Watanabe, Y Yumura, M Takeda, M Hosaka

INTERNATIONAL JOURNAL OF UROLOGY 8 ( 11 ) 615 - 622 2001.11

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Spermatogonial transplantation: the principle and possible applications

T Ogawa

JOURNAL OF MOLECULAR MEDICINE-JMM 79 ( 7 ) 368 - 374 2001.7

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DOI： 10.1007/s001090100228

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Effect of the GnRH-agonist leuprolide on colonization of recipient testes by donor spermatogonial stem cells after transplantation in mice

Dobrinski, I, T Ogawa, MR Avarbock, RL Brinster

TISSUE & CELL 33 ( 2 ) 200 - 207 2001.4

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DOI： 10.1054/tice.2001.0177

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精原幹細胞の継代移植

小川毅彦, 湯村寧, 宇田川幸一, 武田光正, 穂坂正彦

日本泌尿器科学会雑誌 92 ( 2 ) 292 - 292 2001

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DOI： 10.5980/jpnjurol.92.292_2

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抗癌剤による精巣傷害後の精子形成再生に対するGnRH analogueの効果

宇田川幸一, 小川毅彦, 渡辺岳志, 湯村寧, 田中克幸, 武田光正, 穂坂正彦

日本泌尿器科学会雑誌 92 ( 2 ) 410 - 410 2001

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DOI： 10.5980/jpnjurol.92.410_1

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若年癌患者に対する精子凍結保存の経験

齋藤和男, 湯村寧, 小川毅彦, 野口和美, 穂坂正彦, 木下裕三, 岩崎皓, 佐藤和彦, 菅野ひとみ

日本泌尿器科学会雑誌 92 ( 2 ) 216 - 216 2001

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DOI： 10.5980/jpnjurol.92.216_2

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生体腎移植提供者に対するMR-Angiographyの検討

寺西淳一, 槙山和秀, 榛葉隆文, 小川毅彦, 斎藤和男, 野口和美, 増田光伸

移植 35 ( 総会臨時 ) 295 - 295 2000.9

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Transplantation of male germ line stem cells restores fertility in infertile mice

T Ogawa, Dobrinski, I, MR Avarbock, RL Brinster

NATURE MEDICINE 6 ( 1 ) 29 - 34 2000.1

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DOI： 10.1038/71496

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Green fluorescent protein(GFP)マウスを用いた精細管へのGerm Cell Transplantation

湯村寧, 小川毅彦, 武田光正, 田中克幸, 渡辺岳志, 友田岳志, 宇田川幸一, 穂坂正彦

日本泌尿器科学会雑誌 91 ( 3 ) 366 - 366 2000

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DOI： 10.5980/jpnjurol.91.366_3

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ラット実験的腹腔内精巣後の精子形成障害に対するLHRH analogueの効果

宇田川幸一, 小川毅彦, 渡辺岳志, 湯村寧, 田中克幸, 武田光正, 穂坂正彦

日本泌尿器科学会雑誌 91 ( 3 ) 364 - 364 2000

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DOI： 10.5980/jpnjurol.91.364_4

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Computer assisted image analysis to assess colonization of recipient seminiferous tubules by spermatogonial stem cells from transgenic donor mice

Dobrinski, I, T Ogawa, MR Avarbock, RL Brinster

MOLECULAR REPRODUCTION AND DEVELOPMENT 53 ( 2 ) 142 - 148 1999.6

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Xenogeneic spermatogenesis following transplantation of hamster germ cells to mouse testes

T Ogawa, Dobrinski, I, MR Avarbock, RL Brinster

BIOLOGY OF REPRODUCTION 60 ( 2 ) 515 - 521 1999.2

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DOI： 10.1095/biolreprod60.2.515

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Viability and function of human sperm in electrolyte-free cold preservation Reviewed

Hitomi Kanno, Kazuo Saito, Takehiko Ogawa, Mitsumasa Takeda, Akira Iwasaki, Yuzo Kinoshita

Fertility and Sterility 69 ( 1 ) 127 - 131 1998.1

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DOI： 10.1016/S0015-0282(97)00439-1

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器官培養したマウス精巣に対するすだれ状X線マイクロビーム照射と精子形成に与える影響 International coauthorship

福永久典, 神長輝一, 佐藤卓也, 渡辺立子, 宇佐美徳子, 小川毅彦, 横谷明徳, Kevin M. Prise

Photon Factory Activity Report 2018 36 2017G565 2019.7

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泌尿器科領域における基礎研究の成果 In vitro精子形成システムの開発

古目谷暢, 小川毅彦, 矢尾正祐

泌尿器外科 32 ( 臨増 ) 592 - 594 2019.6

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Application of an ex vivo organ culture method to investigate impacts on spermatogenesis following exposure to intensity- and distribution-modulated radiation fields

福永久典, 神長輝一, 宇佐美徳子, 小川毅彦, PRISE Kevin, 横谷明徳

量子ビームサイエンスフェスタ(Web) 2017 2018

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精巣組織器官培養法を用いた放射線照射影響の検討と治療への応用

福永久典, 神長輝一, 佐藤卓也, Butterworth KT, 横谷明徳, 小川毅彦, Prise KM

生命科学系学会合同年次大会 2017年度 4AT180500 2017.12

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精子形成を長期間維持できるin vitro精巣組織培養システムの開発

古目谷暢, 林功晃, 山中弘行, 三條博之, 小島一晃, 片桐久美子, 佐藤卓也, 矢尾正祐, 木村啓志, 藤井輝夫, 小川毅彦

日本生殖内分泌学会雑誌 21 27 - 31 2016.8

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精子無力症患者における精子プロテオーム解析

三條博之, 荒川憲昭, 湯村寧, 矢尾正祐, 小川毅彦

日本泌尿器科学会総会(Web) 104th 2016

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精子幹細胞研究の展望 Invited

佐藤卓也, 小川毅彦

臨床婦人科産科 69 ( 8 ) 786 - 790 2015

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DOI： 10.11477/mf.1409208497

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精子幹細胞と精子形成：ex vivo cultureの可能性 Invited

佐藤卓也, 横西哲広, 小川毅彦

32 859 - 864 2014

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Reply by the authors

Hiroki Ito, Takashi Kawahara, Hideyuki Terao, Junichi Matsuzaki, Takehiko Ogawa, Masahiro Yao, Yoshinobu Kubota

Urology 81 ( 1 ) 217 2013.1

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DOI： 10.1016/j.urology.2012.08.017

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Re: Ito et al.: The Most Reliable Preoperative Assessment of Renal Stone Burden as a Predictor of Stone-free Status After Flexible Ureteroscopy With Holmium Laser Lithotripsy: A Single-center Experience (Urology 2012; 80:524-528) Reply

Hiroki Ito, Takashi Kawahara, Hideyuki Terao, Junichi Matsuzaki, Takehiko Ogawa, Masahiro Yao, Yoshinobu Kubota

UROLOGY 81 ( 1 ) 217 - 217 2013.1

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精子形成不全マウスの精巣組織から培養下での精子産生 Invited

佐藤卓也, 小川毅彦

細胞工学 32 ( 2 ) 212 - 213 2013

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新生仔マウス精巣からの試験管内精子産生 Invited

佐藤卓也, 小川毅彦

BIO Clinica 28 80 - 84 2013

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目で見る生殖幹細胞－精子幹細胞 Invited

佐藤卓也, 小川毅彦

20 ( 3 ) 4 - 7 2013

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精巣組織の凍結 Invited

横西哲広, 古目谷暢, 佐藤卓也, 小川毅彦

HORMONE FRONTIER IN GYNECOLOGY 20 ( 2 ) 169 - 175 2013

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Authors' response to letter to the Editor

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Yoshitake Kato, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

Journal of Endourology 26 ( 7 ) 926 2012.7

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DOI： 10.1089/end.2012.1528

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URETEROSCOPY ASSISTED RETROGRADE NEPHROSTOMY: A NEW TECHNIQUE FOR PERCUTANEOUS NEPHROLITHOTOMY (PCNL) REPLY

Takashi Kawahara, Hiroki Ito, Hideyuki Terao, Takehiko Ogawa, Hiroji Uemura, Yoshinobu Kubota, Junichi Matsuzaki

BJU INTERNATIONAL 109 ( 10 ) E34 - E35 2012.5

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DOI： 10.1111/j.1464-410X.2012.11000_2.x

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極少数(1〜40個)のマウス培養精原幹細胞のマイクロドロップ内での増殖

荒木泰行, 佐藤卓也, 片桐久美子, 窪田吉信, 荒木康久, 小川毅彦

日本生殖医学会雑誌 56 ( 4 ) 326 - 326 2011.10

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in vitroでマウスの精子幹細胞から妊孕能のある精子を産生することに成功

佐藤卓也, 小川毅彦

細胞工学 30 ( 9 ) 980 - 983 2011

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Spermatogonial stem cells and in vitro spermatogenesis

Takehiko Ogawa

Reproductive Medicine and Biology 10 ( 3 ) 175 - 178 2011

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DOI： 10.1007/s12522-011-0084-7

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in vitroでマウスの精子幹細胞から妊孕能のある精子を産生することに成功 Invited

佐藤卓也, 小川毅彦

細胞工学 30 ( 9 ) 212 - 213 2011

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横浜市立みなと赤十字病院開院5年における男性不妊外来患者の統計的観察

竹島徹平, 小林将貴, 逢坂公人, 横溝由美子, 岩崎晧, 湯村寧, 小川毅彦, 齋藤和男, 佐藤和彦

日本生殖医学会雑誌 55 ( 4 ) 401 - 401 2010.10

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当院における前立腺癌に対するIMRTの治療経過

河原崇司, 上村博司, 幡多政治, 関口善吉, 南村和宏, 伊藤悠城, 佐野太, 河路かおる, 村上貴之, 槙山和秀, 中井川昇, 小川毅彦, 矢尾正祐, 寺西淳一, 皆川由美子, 小田切一将, 南澤素子, 野口和美, 井上登美夫, 窪田吉信

泌尿器外科 23 ( 8 ) 1165 - 1166 2010.8

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精子幹細胞の培養と精子形成

小川毅彦

日本生殖内分泌学会雑誌 15 13 - 18 2010

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Mumpus orchitis後の男性不妊症における精液中活性酸素(Reactive oxygen species:ROS)値について

竹島徹平, 湯村寧, 服部裕介, 小林将貴, 小川毅彦, 齋藤和男, 佐藤和彦, 野口和美, 岩崎晧

日本生殖医学会雑誌 54 ( 4 ) 330 - 330 2009.10

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脱血あるいは Winter 法施行後の持続陰茎勃起症3例の治癒過程からの虚血性持続勃起症成立機序への考察

岩崎晧, 小林将貴, 澤田卓人, 湯村寧, 小川毅彦, 斉藤和男, 佐藤和彦

日本性機能学会雑誌 = The japanese journal of Impotence Research 24 ( 2 ) 218 - 219 2009.8

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In vitro murine spermatogenesis from spermatogonia in organ culture system

Takehiko Ogawa, Kumiko Katagiri, Kaoru Kita, Yoshinobu Kubota

BIOLOGY OF REPRODUCTION 230 - 230 2008

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若年男性に発症したrenal cell carcinoma,unclassified(WHO classification)

星野耕二, 蓮見壽史, 村上貴之, 槇山和秀, 吉田実, 三好康秀, 中井川昇, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信, 大城久, 長嶋洋治

泌尿器外科 20 ( 11 ) 1482 - 1482 2007.11

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横浜赤十字病院性機能外来の臨床統計およびクエン酸シルデナフィル治療効果の検討

岩崎晧, 澤田卓人, 林成彦, 湯村寧, 小川毅彦, 斎藤和男, 佐藤和彦

日本性機能学会雑誌 = The japanese journal of Impotence Research 22 ( 2 ) 268 - 268 2007.8

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系統的前立腺12ヶ所針生検におけるPSADの有用性の検討

横溝由美子, 上村博司, 三好康秀, 蓮見壽史, 梅本晋, 杉浦晋平, 槙山和秀, 中井川昇, 小川毅彦, 矢尾正祐, 窪田吉信

泌尿器外科 20 ( 臨増 ) 613 - 613 2007.5

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根治的前立腺全摘除術後の予後とadjuvant療法、PSA再発後のPSA doubling timeとsalvage治療についての検討

三好康秀, 上村博司, 梅本晋, 中村昌史, 杉浦晋平, 蓮見壽史, 村上貴之, 槙山和秀, 藤浪潔, 中井川昇, 小川毅彦, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 98 ( 2 ) 499 - 499 2007.2

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原発巣再発は認めず子宮転移のみを来たした表在性膀胱癌の1例

村上貴之, 星野耕二, 蓮見壽史, 槙山和秀, 三好康秀, 吉田実, 中井川昇, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

泌尿器科紀要 53 ( 1 ) 75 - 77 2007.1

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早期前立腺癌に対するヨード125シード線源を用いた密封小線源治療(ブラキセラピー)の術後経過についての検討

三好康秀, 上村博司, 杉浦晋平, 蓮見壽史, 林成彦, 村上貴之, 槙山和秀, 中井川昇, 小川毅彦, 矢尾正祐, 板澤朋子, 荻野伊知郎, 窪田吉信

日本癌治療学会誌 41 ( 2 ) 722 - 722 2006.9

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Laparoscopic adrenalectomy; Comparison of transperitoneal vs retroperitoneal approach.

Yasuhide Miyoshi, Noboru Nakaigawa, Takeshi Kishida, Kotaro Suzuki, Takayuki Murakami, Masahiro Yao, Hiroji Uemura, Takehiko Ogawa, Yoshinobu Kubota, Kazuhide Makiyama

JOURNAL OF ENDOUROLOGY 20 A101 - A101 2006.8

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BCG膀注後に子宮転移を来たした膀胱癌の1例

村上貴之, 蓮見壽史, 槙山和秀, 三好康秀, 吉田実, 中井川昇, 小川毅彦, 上村博司, 矢尾正佑, 窪田吉信

神奈川医学会雑誌 33 ( 2 ) 227 - 227 2006.7

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後腹膜鏡下手術における腹膜損傷の検討

槙山和秀, 中井川昇, 三好康秀, 吉田実, 村上貴之, 蓮見壽史, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

泌尿器外科 19 ( 臨増 ) 431 - 431 2006.4

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前立腺癌における血清抗p53抗体の予後予測因子としての有用性

三好康秀, 上村博司, 高瀬和紀, 杉浦晋平, 蓮見壽史, 中村昌史, 藤浪潔, 小川毅彦, 矢尾正祐, 窪田吉信

泌尿器外科 19 ( 臨増 ) 452 - 452 2006.4

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腹腔鏡下副腎摘除術経腹膜と後腹膜アプローチ,左右の比較検討

槙山和秀, 中井川昇, 三好康秀, 吉田実, 蓮見壽史, 村上貴之, 星野耕二, 小川毅彦, 上村博司, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 97 ( 2 ) 317 - 317 2006.3

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根治的前立腺全摘術後尿失禁に対する干渉低周波治療についての検討

喜多かおる, 上村博司, 蓮見壽史, 林茂彦, 三好康秀, 小川毅彦, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 97 ( 2 ) 454 - 454 2006.3

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早期前立腺癌に対するヨード125シード線源を用いた密封小線源治療(ブラキセラピー)の術後経過についての検討

三好康秀, 上村博司, 杉浦晋平, 蓮見壽史, 梅本晋, 中村昌史, 板澤朋子, 中井川昇, 小川毅彦, 矢尾正祐, 荻野伊知朗, 窪田吉信

日本泌尿器科学会雑誌 97 ( 2 ) 481 - 481 2006.3

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Proliferation of spermatogonial stem cells and spermatogenesis in vitro

Takehiko Ogawa, Kaoru Kita, Yoshinobu Kubota

Reproductive Medicine and Biology 5 ( 3 ) 169 - 174 2006

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DOI： 10.1111/j.1447-0578.2006.00138.x

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マウス胚性幹細胞（ES）・胚性生殖（EG）細胞の遺伝子発現様式と性染色体構成の関連に関する解析 Reviewed

三瀬名丹, 近藤昌代, 杠美佐子, 目加田和之, 池郁生, 多田高, 小川毅彦, 金谷重彦, 野瀬俊明, 阿部訓也

日本発生生物学会第39 回大会（2006.6.1－3，広島） 2006

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前立腺癌における血清抗p53抗体の予後予測因子としての有用性

三好康秀, 上村博司, 高瀬和紀, 杉浦晋平, 蓮見壽史, 中村昌史, 藤浪潔, 小川毅彦, 矢尾正祐, 窪田吉信

日本癌治療学会誌 40 ( 2 ) 727 - 727 2005.9

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系統的前立腺12ヶ所生検の有用性の検討

杉浦晋平, 上村博司, 三好康秀, 蓮見壽史, 横溝由美子, 梅本晋, 槙山和秀, 岸田健, 小川毅彦, 矢尾正祐, 窪田吉信

泌尿器外科 18 ( 臨増 ) 560 - 560 2005.5

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系統的前立腺12ヶ所針生検の有用性の検討

横溝由美子, 上村博司, 三好康秀, 蓮見壽史, 梅本晋, 杉浦晋平, 槙山和秀, 中井川昇, 小川毅彦, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 96 ( 2 ) 161 - 161 2005.3

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前立腺癌に対するアンドロゲン除去療法症例における血清p53抗体の予後的意義(Prognostic significance of serum p53 antibodies in patients with androgen ablation therapy for prostate cancer)

相浦晋平, 上村博司, 三好康秀, 蓮見壽史, 小川毅彦, 矢尾正祐, 窪田吉信

日本泌尿器科学会雑誌 96 ( 2 ) 419 - 419 2005.3

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Improvement in recipient mouse fertility of spermatogonial transplantation.

T Ogawa, Y Tamura, K Kita, Y Kubota

BIOLOGY OF REPRODUCTION 234 - 235 2005

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自己組織化マッピング法による胚性幹細胞・生殖細胞の発現相互解析 Reviewed

三瀬名丹, 渕上拓也, 杉本道彦, 小早川智, 杠美佐子, 池郁生, 多田高, 小川毅彦, 金谷重彦, 野瀬俊明, 阿部訓也

第28回日本分子生物学会年会（2005.12.7－10. 福岡） 2005

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前立腺癌におけるNAG-1の発現の検討

河原崇司, 上村博司, 石黒斉, 三好康秀, 岸田健, 小川毅彦, 矢尾正祐, 窪田吉信

日本癌学会総会記事 63回 365 - 365 2004.9

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Result of Long-term Methylcobalamin Treatment for Male Infertility

IWASAKI Akira, HOSAKA Masahiko, KINOSHITA Yuzo, SAITO Kazuo, YUMURA Yasushi, OGAWA Takehiko, KANNO Hitomi, SATO Kazuhiko

48 ( 3 ) 119 - 124 2003.10

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46, XYq-with a Chief Complaint of Infertility : A Case Report

YUMURA Yasushi, KANNO Hitomi, OGAWA Takehiko, SAITO Kazuo, SAWADA Takuto, SATO Kazuhiko, HOSAKA Masahiko, IWASAKI Akira

48 ( 3 ) 81 - 86 2003.10

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A Case of Mullerian Duct Cyst with Improvement of Semen Findings after Bilateral Epididymitis Following Transurethral Resection of Hemi-Verumontanum

SAWADA Takuto, IWASAKI Akira, ISHIZUKA Eiichi, YUMURA Yasushi, KANNO Hitomi, OGAWA Takehiko, SAITO Kazuo, SATO Kazuhiko, KUBOTA Yoshinobu

48 ( 3 ) 125 - 129 2003.10

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心因性勃起障害に対するクエン酸シルデナフィル治療の有用性と問題点

岩崎晧, 澤田卓人, 湯村寧, 管野ひとみ, 小川毅彦, 斎藤和男, 佐藤和彦, 窪田吉信, 石塚榮一

日本性機能学会雑誌 = The japanese journal of Impotence Research 17 ( 2 ) 189 - 190 2002.8

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サイトメガロウイルス抗体陽性ドナーから陰性レシピエントへの生体腎移植の検討

寺西淳一, 黒川陽子, 鈴木康太郎, 三好秀康, 小川毅彦, 野口和美, 齋藤和男, 増田光伸

神奈川医学会雑誌 29 ( 1 ) 105 - 106 2002.1

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クエン酸シルデナフィルの治療効果よりみた加齢性勃起障害の臨床像

岩崎晧, 澤田卓人, 石塚榮一, 湯村寧, 菅野ひとみ, 小川毅彦, 斎藤和男, 佐藤和彦, 穂坂正彦

日本性機能学会雑誌 = The japanese journal of Impotence Research 15 ( 2 ) 272 - 273 2000.9

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Language：Japanese

CiNii Books

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Rebound of prostate specific antigen after discontinuation of antiandrogen therapy for benign prostatic hyperplasia

46 ( 9 ) 605 - 607 2000.9

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Language：Japanese

CiNii Books

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Other Link： http://search.jamas.or.jp/link/ui/2001061907
Recipient preparation is critical for spermatogonial transplantation in the rat

T Ogawa, Dobrinski, I, RL Brinster

TISSUE & CELL 31 ( 5 ) 461 - 472 1999.10

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DOI： 10.1054/tice.1999.0060

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外傷・手術を契機とした特異的な射精障害の2例

岩崎晧, 澤田卓人, 湯村寧, 菅野ひとみ, 小川毅彦, 斎藤和男, 佐藤和彦, 穂坂正彦

Impotence : journal of the Society for Impotence Research 14 ( 2 ) 241 - 242 1999.9

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CiNii Books

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Computer assisted image analysis to assess colonization of recipient seminiferous tubules by spermatogonial stem cells from transgenic donor mice

Dobrinski, I, T Ogawa, MR Avarbock, RL Brinster

MOLECULAR REPRODUCTION AND DEVELOPMENT 53 ( 2 ) 142 - 148 1999.6

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DOI： 10.1002/(SICI)1098-2795(199906)53:2<142::AID-MRD3>3.0.CO;2-O

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Development of germ cell transplants: morphometric and ultrastructural studies

GG Parreira, T Ogawa, MR Avarbock, LR Franca, CL Hausler, RL Brinster, LD Russell

TISSUE & CELL 31 ( 3 ) 242 - 254 1999.6

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DOI： 10.1054/tice.1999.0006

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Germ cell genotype controls cell cycle during spermatogenesis in the rat

LR Franca, T Ogawa, MR Avarbock, RL Brinster, LD Russell

BIOLOGY OF REPRODUCTION 59 ( 6 ) 1371 - 1377 1998.12

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Development of germ cell transplants in mice

GG Parreira, T Ogawa, MR Avarbock, LR Franca, RL Brinster, LD Russell

BIOLOGY OF REPRODUCTION 59 ( 6 ) 1360 - 1370 1998.12

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Functional analysis of ZNF85 KRAB zinc finger protein, a member of the highly homologous ZNF91 family

DA Poncelet, EJ Bellefroid, PV Bastiaens, MA Demoitie, JC Marine, H Pendeville, Y Alami, N Devos, P Lecocq, T Ogawa, M Muller, JA Martial

DNA AND CELL BIOLOGY 17 ( 11 ) 931 - 943 1998.11

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Leuprolide, a gonadotropin-releasing hormone agonist, enhances colonization after spermatogonial transplantation into mouse testes

T Ogawa, Dobrinski, I, MR Avarbock, RL Brinster

TISSUE & CELL 30 ( 5 ) 583 - 588 1998.10

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DOI： 10.1016/S0040-8166(98)80039-6

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Enhanced expression in seminoma of human zinc finger genes located on chromosome 19

T Ogawa, DA Poncelet, Y Kinoshita, T Noce, M Takeda, K Kawamoto, K Udagawa, PJ Lecocq, JC Marine, JA Martial, M Hosaka

CANCER GENETICS AND CYTOGENETICS 100 ( 1 ) 36 - 42 1998.1

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DOI： 10.1016/S0165-4608(97)00004-6

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Development of spermatogonial transplants in the mouse testis.

GG Parreira, T Ogawa, MR Avarbock, LR Franca, RL Brinster, LD Russell

BIOLOGY OF REPRODUCTION 58 171 - 171 1998

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Language：English Publishing type：Research paper, summary (international conference)

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Transplantation of testis germinal cells into mouse seminiferous tubules

T Ogawa, JM Arechaga, MR Avarbock, RL Brinster

INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 41 ( 1 ) 111 - 122 1997.2

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AN AUTORADIOGRAPHIC STUDY OF THE RENEWAL OF MOUSE BRONCHIOLAR EPITHELIUM FOLLOWING BROMOBENZENE EXPOSURE

T OGAWA, M TSUBAKIHARA, M ICHIKAWA, M KANISAWA

TOXICOLOGIC PATHOLOGY 21 ( 6 ) 547 - 553 1993.11

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Extracorporeal shock wave lithotripsy for urinary tract stones using piezoelectric lithotripter(piezolith)

OGAWA Takehiko

Acta Urol. Jpn. 38/,1-4 1 - 4 1992

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Awards

Johnson & Johnson Innovation Award

2013.3

OGAWA Takehiko

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ゴールドメダル賞

2012.4 読売テクノフォーラム「培養下での精子形成法の開発」

小川毅彦

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Research Projects

Developing an in vitro system for human spermatogenesis

Grant number：22H00485 2022.4 - 2025.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

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Grant amount：\41860000 （ Direct Cost: \32200000 、 Indirect Cost：\9660000 ）

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Establishment of in vitro spermatogenesis with high integrity

2018.7 - 2022.3

OGAWA Takehiko

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Authorship：Principal investigator Grant type：Competitive

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動物における配偶子産生システムの制御

Grant number：18H05187 2018.4 - 2019.3

日本学術振興会科学研究費助成事業新学術領域研究(研究領域提案型)

小林悟, 小川毅彦

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Grant amount：\3900000 （ Direct Cost: \3000000 、 Indirect Cost：\900000 ）

本研究領域では、始原生殖細胞を作り出すメカニズム、および配偶子幹細胞(GSC)の働きにより配偶子を継続して産生するメカニズムの解明を目指し研究を遂行し、多くの研究成果が蓄積されてきた。得られた成果が、当該研究分野や関連分野において周知され、新たな研究領域を形成する核となるべく、研究成果を取りまとめた。具体的には、５年間にわたる計画研究および２年間の公募研究で得られた研究成果をとりまとめた。得られた研究成果を学術雑誌や学会などで発表するとともに、研究成果を取りまとめた冊子を編集した。この冊子を、関連分野の研究者に配布し、本領域の成果について広く発信する。

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Investigation of bone microenvironment using 3D-organoid model.

Grant number：17H04330 2017.4 - 2020.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

MATSUYAMA Hideyasu

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Grant amount：\17030000 （ Direct Cost: \13100000 、 Indirect Cost：\3930000 ）

We established a novel 3D in vitro culture, a mimicry of bone microenvironment (GFP-transferred C4-2 [CRPC cell line] onto RFP-transferred human osteoblast differentiated from human-mesenchymal stem cell in chitosan nanofiber-coated culture plate). After 15 days incubation, drug susceptibilities of enzalutamide, apalutamide, darolutamide, and abiraterone (Abi) with/without dutasteride (Duta) were evaluated under androgen deprivation condition. As for IC 50 of each drug, Abi+Duta combination was the lowest, and darolutamide, Abi, enzalutamide, apalutamide was lower in order. IC50 of Abi+Duta combination was identical to that of delta-4 abiraterone, a metabolite of Abi. These data suggest colony inhibition effect of combination was attributable to delta-4 abiraterone.

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International joint research on gamete formation through the cutting-edge analysis system

Grant number：15K21736 2015.11 - 2018.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Kobayashi Satoru, MATSUI YASUHISA, YOSHIZAKI GORO, OBATA YAYOI

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Grant amount：\35100000 （ Direct Cost: \27000000 、 Indirect Cost：\8100000 ）

International joint research was performed, based on the research supported by Grant-in-Aid for Scientific Research on Innovation Area "Mechanisms regulating gamete formation in animals", which aimed to find the regulatory mechanisms of primordial germ cell (PGC) formation and gamete stem cell (GSC) function in animals. This international joint research consisted of constructing an in vitro live-imaging system for spermatogenesis, developing the method to analyze epigenetic regulation of spermatogenesis, and encouraging and supporting tight interaction with oversea researchers through international meetings. This research grant enabled us to establish a novel world-leading research community.

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Development of in vitro embryo culture system based-on microfluidics for artificial reproductive technology

Grant number：26390039 2014.4 - 2017.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

Kimura Hiroshi, FUJII TERUO, OGAWA TAKEHIKO, NAKAMURA HIROKO, TAKAHASHI TSUBASA

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Grant amount：\5070000 （ Direct Cost: \3900000 、 Indirect Cost：\1170000 ）

We aimed to develop an in vitro embryo production device which allows three steps of sperm selection, fertilization and culture on a microdevice. We have integrated the sperm sorting function combining with swim-up and swim-down methods onto the device. To evaluate the device functions, sperm sorting experiment, in vitro fertilization experiment, and embryo culture experiment have been carried out. As results, we concluded that the device has ability to produce high quality embryos by integrating the sperm sorting function. This concept will open and enhance the management of an individual embryo for assisted reproductive technology, livestock breeding, and fundamental stage research by further development.

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in vitroにおいて継続的に精子を産生する新規培養系の開発

2013.7 - 2018.3

文部科学省新学術領域提案型科学研究費補助金

小川毅彦

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Authorship：Principal investigator Grant type：Competitive

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Mechanisms regulating gamete formation in animals

Grant number：25114001 2013.6 - 2018.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Kobayashi Satoru, OHBO KAZUYUKI, OBATA YAYOI

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Grant amount：\71370000 （ Direct Cost: \54900000 、 Indirect Cost：\16470000 ）

The Grant-in-Aid for Scientific Research on Innovation Area "Mechanisms regulating gamete formation in animals" aimed to find the regulatory mechanisms of primordial germ cell (PGC) formation and gamete stem cell (GSC) function in diverse animal species. To accomplish this purpose, the researchers using unique organisms, and with different backgrounds, such as in vivo and in vitro analysis, cooperated with each other. This committee encouraged and supported tight interactions within the researchers to establish a novel world-leading research community.

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器官培養法を用いたヒトin vitro精子形成法の開発

Grant number：24930032 2012.4 - 2015.3

文部科学省科学研究費補助金基盤(Ｂ) 奨励研究

小川毅彦

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一昨年、私たちの研究チームは、マウス精巣の親織片をアガロースゲル上で培養することにより、精子形成を誘導し、精子幹細胞からの精子産生に成功した。これは器官培養法の基本である気層と液層の境界部に組織蹄を置く培養法を場いたものであるが、幾つかの工夫を加えて簡便な実験系を作った二とは始まり、最終的には、血清に代わる添加物を加えるたことが成功に結び付いた。この方法がヒト精子形成にも応用できるか否かが、今回の研究の要点である。
ヒト精巣組織検体は、精巣腫瘍患者の手術時に摘出される精巣に部分的に残存する正常組織を用いた。不妊症患者の精巣生検における採取は、今回は適切な症例がなかったために行わなかった。ヒト精巣組織(成人)を1.5%アガロースゲル上に乗せ、培養液には、αMEM/10%KSR、あるいはαMEM/AlbuMAX(40mg/ml)を用いた。培養1～2か月後に、組織片を回収し、ブアン液で固定し、HE染色で観察した。ほとんどの精巣組織は、強い線誰化を生じており、中心部は壊死様になっていた。精細管構造も線維化により不明瞭になっている部分がほとんどであった。精子形成の進行は望めない状況と思われた。培養液に、テストステロン、LH、FSH、などのホルモン、レチノイン酸その他のビタミンを添加したが現在豪でのところ精子形成を促進する効果は確認できていない。BrdUを添加し、減数分裂前の精原細胞が減数分裂に入るかを確認する実験でも残念ながら減数分裂像は認められなかった。培養液のさらなる工夫が必要であると考えられた。

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In vitro system for spermatogenesis from spermatogonial stem cells

Grant number：21592080 2009.4 - 2012.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

OGAWA Takehiko, YUMURA Yasushi

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Authorship：Principal investigator Grant type：Competitive

In vitro reconstitution of spermatogenesis is a very difficult task and remained unsuccessful for a long time. We challenged that task using classical organ culture method, called air-liquid interphase. Through testing variety of culture conditions, we found that a serum replacement, KSR, is effective and induced complete spermatogenesis of mouse. Offspring were obtained with the sperm produced in vitro. We also showed that cryopreservation of testis tissue fragment is possible to preserve spermatogenic ability of the tissues.

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Regulatory Mechanism of Gamete Stem Cells

Grant number：20116001 2008.11 - 2014.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

YOSHIDA Shosei, KOBAYASHI Satoru, YOSHIZAKI Goro, KOBAYASHI Kazuya, NIKI Yuzo, OGAWA Takehiko, OHBO Kazuyuki, ASAOKA Miho

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Grant amount：\53300000 （ Direct Cost: \41000000 、 Indirect Cost：\12300000 ）

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Reconstruction of Drosophila GSC/niche system in vitro and analysis of GSC differentiation

Grant number：20116003 2008.10 - 2013.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

NIKI YUZO

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Authorship：Principal investigator Grant type：Competitive

We succeeded in the reconstitution of GSC/niche system in Drosophila. We established stable cell lines originated from female and male niche cells (cap cells and escort cells in female, and hub and cyst cells in male). Using these cell lines, we identified highly expressed genes in each cell line and found that there are many unique genes specifically expressed in each niche cell line. Furthermore, we found that Drosophila insulin-like genes are also expressed in the niche cells. We constructed co-culture system of GSCs and niche cells and analyzed roles of growth factors. Furthermore, we succeeded in artificial induction of the differentiation and dedifferentiation of GSCs by coculturing with niche cells.

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霊長類の生殖幹細胞株(GS細胞)樹立にむけた幹細胞純化と異種移植系の開発

Grant number：19659045 2007 - 2008

日本学術振興会科学研究費助成事業萌芽研究

大保和之, 小川毅彦

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Grant amount：\3300000 （ Direct Cost: \3300000 ）

(1)異種移植系の確立
昨年度、全身にGFPを発現するウサギ由来精巣を、NOGマウスをrecipientとして精細管移植したところ、GFP陽性のウサギ由来雄生殖細胞が、nudeマウスに比較して高率に精細管に認められたが、NOGマウスは精巣に浮腫を起こしやすいという問題があることがわかった。そこで移植拒絶の原因であるNK細胞を欠損、リンパ球が著減しているIL-2Rγ/RAG2欠損マウスを導入し、同様に精細管移植を行なったが、期待されるような高い移植成績は得られなかった。そこで、近年NOGマウスに匹敵するヒト組織が効率よく生着する新たな遺伝子改変マウスが樹立されており,今後異種精細管移植に使用する予定である。
(2)精巣幹細胞株樹立に向けた、大型動物由来精原細胞の特性の解析
マウス以外の動物よりCell Sorterを用いて精原細胞を粗精製し、マウスに準じた培養を行なったが、精原細胞は増殖しなかった。この原因としてサイトカインの種特異性や、雄生殖細胞分化プロセスの共通性の欠如など様々な原因が考えられた。そこで、近年ES細胞、ips細胞研究でゲノム修飾酵素を制御することにより樹立効率を上げることが行なわれていることから、精巣幹細胞株樹立に応用可能か精巣幹細胞のゲノム修飾状態を解析した。その結果、精原細胞はゲノム低メチル化とH3Me2K9修飾の欠損を特徴とした。また組織学的に各動物の精巣組織像を比較検討したところ、マーモセット、ウサギはマウスに類似し、ブタは極度に発達したライディッヒ細胞と多層の精細管群を特徴としマウスと大きく異なっていた。今後これらの結果を応用し、マウス類似の組織構造を持つ動物に焦点を絞り、ゲノム修飾酵素の制御を視野に、種を超えた精巣幹細胞培養法を検討する。

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ex vivo spermatogenesis from cultured spermatogonial stem cells

Grant number：18591783 2006.4 - 2008.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

OGAWA Takehiko

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1) Establishement and maintenance of mouse spermatogonial stem cell lines:
Three transgenic mice (Tg) lines were used; ubiquitous GFP expressing Tg, Acrosin-GFP Tg expressing GFP at mid-meiosis onward, and Haspin-GFP Tg expressing GFP at haploid stage. Spermatogonial stem cell lines, also called germline stem cells (GS cells) were established from these three Tg mice.
2) Ex vivo spermatogenesis from GS cells:
In order to induce spermatogenesis form GS cells in vitro, GS cells of Acrosin-GFP Tg and Haspin-GFP Tg were cultured in various conditions such as different feeder cells. So far, however, GFP expression, marker of meiosis, was not detected in our experience.
In our trial for ex vivo spermatogenesis, we have found that seminiferous tubules can be reconstituted de novo from dissociated fetal or neonatal testis cells in the subcutis of nude mice. We took advantage of the reconstitution ability of immature testis cells to get GS cells integrated in them. Those GS cells underwent differentiation up to spermatid (haploid) stage which was used for insemination to end up produce healthy pups.
3) Organ culture of testis tissues:
Our experience of cell culture for spermatogenesis was rather discouraging and it becomes evident for us to convert to another strategy. Using acrosin- and haspin-GFP Tg mice testis tissue, 3-14 days old, we evaluated organ culture method. When 7 or more days old pups were used, haspin-GFP become positive in a culture condition. Basic favorable culture condition includes 32℃, 10% fetal bovine serum, vitamins, and so on. Further improvement could be possible in near future.

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Searching for culture condition optimal for proliferation of spermatogonial stem cells

Grant number：15591702 2003 - 2004

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

OGAWA Takehiko

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Grant amount：\2100000 （ Direct Cost: \2100000 ）

Culturing of Spermatogonial Stem Cells
In April, 2003, it was reported that male germline stem cells, collected from neonatal mouse testes (strains of DBA/2 and ICR), were cultured for long period on feeder layer of mouse embryonic fibroblasts with 4 growth factors (EGF, bFGF, LIF, GDNF). We immediately repeated and confirmed results of the report and progressed it one step beyond by establishing germline stem cells (GS cells) from not only neonates but also from adult mice. The GS cells expanded exponentially in vitro. They produce sperm when transplanted recipient mouse testes. They did not produce tumor nor showed abnormal proliferation in the body of recipient mice. The GS cells was re-derived from recipient mouse testes, which showed that GS cells in vitro and spermatogonial stem cells in the testis were identical or mutually convertible.
Fertilization of recipient mice with spermatogonial transplantation
In order to make recipient mice fertile after spermatogonial transplantation, immature mice (10-15 days old) were treated with irradiation before transplantation. The aim of irradiation was to make empty niche in the testis by removing endogenous germ cells for efficient colonization of donor stem cells. The dose of irradiation turned out to be optimal at around 12Gy for that purpose. One to Three days after the irradiation, the recipient mice received spermatogonial transplantation. Two months after the transplantation onward, the recipient mice were mated with females to test their fertility. It was confirmed that mice became fertile fathered pups of donor germ cell origin at high rate (about 70%). When we used GS cells, the fertility rate of recipient mice became higher, around 80%. The important factors for making recipient fertile with donor germ cells therefore include age of recipient, stem cell concentration of donor cell suspension.

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再生医学（腎再生、膀胱再生）

2002

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Grant type：Competitive

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Searching of factors facilitate spermatogonial stem cell proliferation (Investigation with transplantation and in vitro analysis)

Grant number：13671663 2001 - 2002

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

OGAWA Takehiko

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Grant amount：\2400000 （ Direct Cost: \2400000 ）

1) Fertilization of recipient mice with donor germ cells
Pup mice, aged 8 〜 20 days old, were treated with regional radiation to eliminate innate testis germ cells. Spermatogonial transplantation was performed on those mice. Donor spermatogenesis took place in the host testes pervasively at 1 〜 2 months later. Mating with regular females produced donor germ cell derived progeny. This result indicates that spermatogonial stem cells in the pup testis expanded more rapidly than when they were transplanted in the about recipients.
2) GnRH analogue effects on spermatogonial cell proliferation
We analyzed proliferation of spermatogonia using transplantation and confocal laser microscopic analysis. In GnRH analogue treated mice, the number of spermatogonia significantly increased compared to those in control mice, indicating that GnRH analogue enhanced proliferation or suppressed apoptosis of the spermatogonia. Another experiment using S1/S1^d mice as recipients showed that the effect of GnRH analogue is independent from SCF signal.
3) Culture of spermatogonial stem cells
Testis cells of GFT mice were cultured for 2 to 3 weeks. To confirm the presence of spermatogonial stem cells, they were transplanted to the testes of pup mouse. Several different culture conditions were tested, including feeder cells (STO, 15P-1), coated dishes with lamina or collagen, madrigal matrix, and so on. Among them cultured cells on the madrigal matrix showed they contained spermatogonial stem cells for such periods.

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Analysis of spermatogonial proliferation with technique of spermatogonial transplantation-A trial for improving spermatogenic activity

Grant number：11671569 1999 - 2000

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

OGAWA Takehiko, HOSAKA Masahiko, TAKEDA Mitsumasa

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Grant amount：\2200000 （ Direct Cost: \2200000 ）

Spermatogonial transplantation has several unique advantages when applied to the study of spermatogenesis. For instance, germ cells and testis environment composed of many somatic cells can be differently treated to see effects of the treatments. The proliferation status of spermatogonia can be also clearly observed in the early stage of colony development after transplantation. We have first introduced GFP mice in this system as donor mice by which the system became more quantitative. With GFP mice as donor, sequential transplantation of spermatogonial stem cell became possible. We have so far succeeded to passage stem cells for 4 times successively. Accumulated data indicated that spermatogonial stem cells expand as colony size increases, and as time passes by. The expansion rate was estimated to be about 30-fold during 100 days following transplantation. This expansion rate appeared to be constant for more than a year showing no sign of exhaustion. Meanwhile, we also tried to find the effect of intra-testicular microenvironment on spermatogonial proliferation. The LH-RH analogue (leuprorelin) was used to modify hormonal environment of the testis. In the leuprorelin-treated mouse testes, transplanted speramtogonia presented more densely on the basement membrane of the seminiferous tubules as early as 4 weeks after the transplantation. This finding suggests that spermatogonia are stimulated to proliferate or their apoptosis are reduced in the leuprorelin-treated mouse testes. Our present research established the spermatogonial transplantation technique as a quantitative method for evaluating various treatments on spermatogonial proliferation, differentiation, and death.

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精原幹細胞の培養とin vitro 精子形成

1995

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Grant type：Competitive

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男子不妊症

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Grant type：Competitive

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精巣腫瘍の細胞学的研究

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Grant type：Competitive

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